• Journal of Microbiology and Biotechnology
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• v.25 no.3
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• pp.358-365
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• 2015

ε-Poly-L-lysine (ε-PL) is a homopolymer of L-lysine molecules connected between the epsilon amino and alpha carboxyl groups. This polymer is currently used as a natural preservative in food. Insufficient biomass is a major problem in ε-PL fermentation. Here, to improve cell growth and ε-PL productivity, various nitrogen-rich nutrients were supplemented into flask cultures after 16 h cultivation, marking the onset of ε-PL biosynthesis. Yeast extract, soybean powder, corn powder, and beef extract significantly improved cell growth. In terms of ε-PL productivity, yeast extract at 0.5% (w/v) gave the maximum yield (2.24 g/l), 115.4% higher than the control (1.04 g/l), followed by soybean powder (1.86 g/l) at 1% (w/v) and corn powder (1.72 g/l) at 1% (w/v). However, supplementation with beef extract inhibited ε-PL production. The optimal time for supplementation for all nutrients examined was at 16 h cultivation. The kinetics of yeast-extract-supplemented cultures showed enhanced cell growth and production duration. Thus, the most commonly used two-stage pH control fed-batch fermentation method was modified by omitting the pH 5.0-controlled period, and coupling the procedure with nutrient feeding in the pH 3.9-controlled phase. Using this process, by continuously feeding 0.5 g/h of yeast extract, soybean powder, or corn powder into cultures in a 30 L fermenter, the final ε-PL titer reached 28.2 g/l, 23.7 g/l, and 21.4 g/l, respectively, 91.8%, 61.2%, and 45.6% higher than that of the control (14.7 g/l). This describes a promising option for the mass production of ε-PL.

• The Research Journal of the Costume Culture
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• v.9 no.3
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• pp.458-484
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• 2001

The costume originated from the idea that it could protect human beings from ferocious beasts. The military costume was from protection against an enemy equipped with weapons. Furthermore, the makers of the costume were afraid of supernatural powers and it made them devise an armour and decorate it by the concept of superstition for the purpose of overcoming it. The function of military uniform was important for increasing the protection against any enemies while they join in battle. However in harmonious time, it represented artwork of peace, dignity, and order. Since the uniform was a king of symbol to show their position, and their role. They could feel proud as a member of a military group they belong to and they gain honor, courage, patriotic sentiment through their military costume. The purpose of this study was to investigate the originality the military costume as it changed and developed continuously from ancient Egypt, Greece and Rome to modern times. This study was performed to show the comparison and characteristic marks of the past, and the influence of them on the modern military uniform. The method used in this study was using past manuscripts of the early times, information attained from domestic and foreign military documents and previously written reports. As a result, the military costume was one of many means of expression of their natural and social environment. It can be said that the most epoche-marking and powerful changes to the social environment are revolution or war. War absolutely has influenced on the changes of the changes of costume and the military armour according to the type of war and the development of weapons. Especially using the amour, the main purpose that of protecting the body has changed into distinguishing our forces from the enemy and instilling fear into enemy, Furthermore, the class who has the power has used it in order to express their power and dignity. Therefore, armour had required two rules to not only protect the body but also discriminating between classes.

• Journal of the Korea Fashion and Costume Design Association
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• v.10 no.1
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• pp.69-78
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• 2008

A new coinage which calls a modern was appeared variously by values, taste, feature of lifestyle and the meaning of a new coinage has influence in the change of lifestyle. Particularly a popular star is a person who situates in the center of a new coinage, his role, behavior, and appearance have great influence in the birth of a new coinage. He is a fashion leader as well as the object of fashion imitation to the new generation being sensitive for fashion. As his fashion is appeared with important fashion icon in fashion market, fashion companies have developed actively star marketing marking him as an advertising model. Therefore, because the fashion trends of a popular star introduce season's fashion trends and can be important factor forecasting fashion behavior of consumer, this study aims at analysis for fashion trends of a popular star including a public entertainer. The dates of this study were collected by TV drama, internet, and magazines, etc. The fashion trends were divided into feminine fashion trend, sexy fashion trend, tomboy fashion trend, and retro fashion trend. Feminine fashion trend was based on feminity but it was classified in masculine look, simple look, chic look, and natural & romantic look according to the standard of a transformation of feminine image. Sexy fashion trend was divided into two sexy images according to the method wrapping oneself in a dress and the method exposing a specific body part. Tomboy fashion trend could be the immature boyish style which concealed bust or hip or pelvis symbolizing feminity. Retro fashion trend could be style being based on 1980's style and Y line. Fashion items as leggings, baby doll dress, tunic style, dot pattern, garsonne look were come into fashion.

• The Plant Pathology Journal
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• v.25 no.2
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• pp.136-141
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• 2009

Marking biocontrol bacteria is an essential step to monitor bacterial behavior in natural environments before application in agricultural ecosystem. In this study, we presented the simple green fluorescent protein (GFP) reporter system driven by the promoter active in Bacillus species for tagging of the biocontrol bacteria. A constitutive promoter P43 from Bacillus subtilis was fused to an enhanced promoterless gfp gene by overlap extension PCR. The GFP expression was demonstrated by the high fluorescence intensity detected in B. subtilis and Escherichia coli transformed with the P43-gfp fusion construct, respectively. The GFP reporter system was further investigated in two bacterial biocontrol strains B. licheniformis and Pseudomonas fluorescens. When the reconstructed plasmid pWH34G was introduced into B. licheniformis, GFP level measured with the fluorescence intensity in B. licheniformis was almost equivalent to that in B. subtilis. However, GFP expression level was extremely low in other biocontrol bacteria P. fluorescens by transposon based stable insertion of the P43-gfp construct into the bacterial chromosome. This study provides information regarding to the efficient biomarker P43-gfp fusion construct for bio-control Bacillus species.

• Journal of Korean Society of Transportation
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• v.20 no.5
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• pp.55-66
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• 2002

In many countries the principle of shielding is employed to permit a more logical approach to restricting new construction and prescribing obstacle marking and lighting. It also reduces the number of cases of new construction requiring review by authorities. Shielding principles are employed when some object, an existing building or natural terrain, already penetrates above one of the obstacle limitation surfaces described in Annex 14. If it is considered that the nature of an object is such that its presence may be permanent, then additional objects within a specified area around it may be permitted to penetrate the surface without being considered as obstacle. The shielding effect of immovable obstacles laterally in final approach and missed approach areas is more uncertain. In certain circumstances, it nay be advantageous to preserve existing unobstructed cross section areas, particularly when the obstacle is close to the runway. This would guard against future changes in either approach or take-off climb area specifications or the adoption of a turned take-off procedure.

• Journal of Ecology and Environment
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• v.41 no.12
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• pp.345-350
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• 2017

Background: Ecological research often requires monitoring of a specific individual over an extended period of time. To enable non-invasive re-identification, consistent external marking is required. Treefrogs possess lateral lines for crypticity. While these patterns decrease predator detection, they also are individual specific patterns. In this study, we tested the use of lateral lines in captive and wild populations of Dryophytes japonicus as natural markers for individual identification. For the purpose of the study, the results of visual and software assisted identifications were compared. Results: In normalized laboratory conditions, a visual individual identification method resulted in a 0.00 rate of false-negative identification (RFNI) and a 0.0068 rate of false-positive identification (RFPI), whereas Wild-ID resulted in RFNI = 0.25 and RFNI = 0.00. In the wild, female and male data sets were tested. For both data sets, visual identification resulted in RFNI and RFPI of 0.00, whereas the RFNI was 1.0 and RFPI was 0.00 with Wild-ID. Wild-ID did not perform as well as visual identification methods and had low scores for matching photographs. The matching scores were significantly correlated with the continuity of the type of camera used in the field. Conclusions: We provide clear methodological guidelines for photographic identification of D. japonicus using their lateral lines. We also recommend the use of Wild-ID as a supplemental tool rather the principal identification method when analyzing large datasets.

• Journal of Ecology and Environment
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• v.43 no.4
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• pp.385-389
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• 2019

Knowledge of abundance, or population size, is fundamental in wildlife conservation and management. Camera-trapping, in combination with capture-recapture methods, has been extensively applied to estimate abundance and density of individually identifiable animals due to the advantages of being non-invasive, effective to survey wide-ranging, elusive, or nocturnal species, operating in inhospitable environment, and taking low labor. We assessed the possibility of using coat patterns from images to identify an individual leopard cat (Prionailurus bengalensis), a Class II endangered species in South Korea. We analyzed leopard cat images taken from Digital Single-Lense Relfex camera (high resolution, 18Mpxl) and camera traps (low resolution, 3.1Mpxl) using HotSpotter, an image matching algorithm. HotSpotter accurately top-ranked an image of the same individual leopard cat with the reference leopard cat image 100% by matching facial and ventral parts. This confirms that facial and ventral fur patterns of the Amur leopard cat are good matching points to be used reliably to identify an individual. We anticipate that the study results will be useful to researchers interested in studying behavior or population parameter estimates of Amur leopard cats based on capture-recapture models.

• Journal of Plant Biology
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• v.9 no.1_2
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• pp.25-30
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• 1966

The writer presented in the report 7th of this series(Chong, 1959. '62 a.b. '64, '65) the result of fertlizer application on Sasamorpha purpurascens var. borealis with regards to soil conditions, climate, planting position etc. and this report is on the effectiveness of fertilizers by kinds of the new (1-year old) and old(3-to 4 years old) Phyllostachys retticulata. The ineffectiveness of fertilizers for old bamboos aged more than 8-years old has been reported previously. The study site was chosen in a bamboo groven situated at Hyunnae Ri, Okkye Myun, Myungju Gun, Kangwon Do. Each plot was so designed that only one individual remained at the center of a circular area with a dimeter of meters by eliminating the clums and severing the rhizomes, of other individuals around it. And, for the fertilizer test, 10 individuals(the new and old: 5 to 5) were observed per kind of fertilizer used; thus marking the total number of 160 plots in all. The second part of this study was conducted for the potted individuals which numbered 48, successfully established ones out of 55 the transplanted to pots. The 48 were divided into 8 treatments: whole fertilization (NPK＋compost＋human manure); NPK; N; K; Human manure; compost; and the control. Amoung of fertilizers applied was 200 grams for the natural plots and 100 grams for pot; it continued for 3 years and applied twice a year (spring and summer) following the theory of Dr. Koichi-ro Ueda(1954, '60 a.b.). Measurements were taken in August each year. The resutls were confirming the fact that the bamboos require a great deal of fertilizers. The results show the following points: 1) Fertilizers were more effective in the 3 to 4 year old bamboos. 2) The order of effectiveness of fertilizers by kind was the whole fertilization, NPK, and N treatments for Phyllostachys reticulata. 3) Organic matter in addition to commercial fertilizers showed better result than the soil application of the latter due to better soil aeration.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.334-334
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• 2022

Improvement of old-fashioned rain gauge systems for automatic, timely, continuous, and accurate precipitation observation is highly essential for weather/climate prediction and natural hazards early warning, since the occurrence frequency and intensity of heavy and extreme precipitation events (especially floods) are recently getting more increase and severe worldwide due to climate change. Although rain gauge accuracy of 0.1 mm is recommended by the World Meteorological Organization (WMO), the traditional rain gauges in both weighting and tipping bucket types are often unable to meet that demand due to several existing technical limitations together with higher production and maintenance costs. Therefore, we aim to introduce a newly developed and cost-effective hybrid rain gauge system at 0.1 mm accuracy that combines advantages of weighting and tipping bucket types for continuous, automatic, and accurate precipitation observation, where the errors from long-term load cells and external environmental sources (e.g., winds) can be removed via an automatic drainage system and artificial intelligence-based data quality control procedure. Our rain gauge system consists of an instrument unit for measuring precipitation, a communication unit for transmitting and receiving measured precipitation signals, and a database unit for storing, processing, and analyzing precipitation data. This newly developed rain gauge was designed according to the weather instrument criteria, where precipitation amounts filled into the tipping bucket are measured considering the receiver's diameter, the maximum measurement of precipitation, drainage time, and the conductivity marking. Moreover, it is also designed to transmit the measured precipitation data stored in the PCB through RS232, RS485, and TCP/IP, together with connecting to the data logger to enable data collection and analysis based on user needs. Preliminary results from a comparison with an existing 1.0-mm tipping bucket rain gauge indicated that our developed rain gauge has an excellent performance in continuous precipitation observation with higher measurement accuracy, more correct precipitation days observed (120 days), and a lower error of roughly 27 mm occurred during the measurement period.

• The Journal of Korean Academy of Prosthodontics
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• v.37 no.4
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• pp.516-530
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• 1999

The current clinical technique for occlusal vertical dimension recording is based on marking the skin reference points on the patient's face and measuring between these points using caliper-like device. And it is difficult to achieve reliable measurements by this technique because of movable soft tissue. The purpose of this study is to reveal the stability of skin reference points by comparing the relative movement between extra-oral skin reference points and intra-oral reference points using X-ray fluoroscope. 10 test subjects were divided into 2 groups : Group I (natural dentition) and Group II (denture-wearer whose vertical dimension was lost) and Group III consists of identical test subjects to Group II with their upper denture removed and record base inserted. Attaching the 3 mm diameter steel ball to nose tip, lower lip, chin and to existing denture (or record base), fluoroscopic examination and recording were taken during 2 jaw opening and closing movements. After subsequent digitization using personal computer, 1219 still pictures with 0.1 second interval were made. Using the 2 dimensional graphic software, measurements between reference points were executed. Dividing the entire jaw movement into 3 ranges (total, 1st half opening, 2nd half opening), rate of movement and relative movement between extra-oral and intra-oral reference points were calculated and statistically analyzed. The results of this study are as follows. 1 Within the same experimental group, no statistical difference was found in the stability of skin reference between lower lip point and chin point during total range of jaw opening and closing movement (p>.05) 2. In the first half range of jaw opening, statistical difference was found between Group I (natural dentition) and Group II (denture wearer) (p<.05) Group I has greater skin reference stability than Group II. 3. In the first half range of jaw opening, statistical difference was found between Group I and Group III (record base wearer) (p<.05). Group I has greater skin reference stability than Group III. 4. In the first half range of jaw opening, no statistical difference was found in the stability of skin reference between Group II and Group III (p>.05). 5. In the second half range of jaw opening, no statistical difference was found in the stability of skin reference between any experimental groups (p>.05). 6. In patients with their occlusal vertical dimension lost, employing other measuring references rather than skin is recommended because of low stability.