• Natural Product Sciences
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• v.11 no.3
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• pp.139-144
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• 2005

Bioflavone quercetin is believed to play an important role preventing bone loss by affecting osteoclastogenesis and regulating many systemic and local factors including hormones and cytokines. This study examined how quercetin acts on tumor necrosis factor-alpha ($TNF-{\alpha}$)-mediated apoptosis in MC3T3-E1 osteoblastic cells. Apoptosis assays revealed the dose-dependent acceleration of quercetin on $TNF-{\alpha}-induced$ apoptosis in MC3T3-E1 cells, which was demonstrated by the increased number of positively stained cells in the trypan blue staining and TUNEL assay, and the migration of many cells to the $sub-G_0/G_1$ phase in flow cytometric analysis. In particular, quercetin treatment alone increased the expression of p53 and p21 proteins in the cells. Consequently, this study showed that quercetin accelerates the $TNF-{\alpha}-induced$ apoptosis in MC3T3-E1 osteoblastic cells.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.756-764
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• 2009

Provision of an optimal environment for the calf is critical to establishing the patterns of growth and development essential to allow the heifer to express its genetic potential for milk output and reproductive capacity during its productive life. Maternal nutrition during gestation is now recognised as a key to genetic programming in utero and this influence is extended through the complexity of hormones, growth factors and immunostimulants incorporated into colostrum and milk consumed by the neonatal calf. This natural process is most often disrupted as calves are weaned abruptly to maximise milk output for commercial exploitation. The key then is to accelerate the rate of maturation of the ruminal epithelium through the provision of concentrate starter rations and high quality forage, which promote VFA production. Management systems to promote these processes in Holstein Friesian cattle are well developed, however, little is known of these processes with buffalo and Bos indicus dairy cattle such as the Sahiwal. The development of methods to program the neonate to grow faster to puberty in these species will be important to improving their productivity for the dairy industries in tropical and sub-tropical environments in the future.

• Journal of Korean Medical classics
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• v.26 no.3
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• pp.85-98
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• 2013

Subject : The application of eight extra Meridians theory to birth physiology of Korean Medicine. Objective : This study suggests physiological theories in child birth in Korean Medicine. Method : First, the application of eight extra Meridians and extraordinary organs theories to physiology, pathology, pregnancy, postnatal period of women were examined based on major publications and theories of some historical characters. This work provided clues for application of eight extra Meridians theory to birth physiology. Second, the progressing birth physiology of modern medicine based on the importance of the brain function was examined, with which the application of eight extra Meridians and extraordinary organs theories to the birth process showed close relationship. Conclusion : The following conclusions could be drawn. In traditional publications of Korean Medicine, the contents explaining birth physiology with eight extra Meridians theory were scarce, mainly due to the doctor's perception of birth as part of the natural female physiology along with menstruation and pregnancy. However, the contents explaining menstruation, pregnancy, postnatal physiology and pathology through eight extra Meridians theory could be found in classical texts such as Neijing, Jebyeong-wonhuron, Imjeunginameuian. Therefore, it is reasonable to apply the eight extra Meridians theory to the birth process as well. Modern medicine's understanding that pituitary and hypothalamus hormones are in play on womb in the birth process, such as Oxytocin, is in continuous progress. Moreover, the environmental condition that has been traditionally known ideal to the birth process in both the East and West-dark, warm, quiet and secluded-is being confirmed in modern research. The application of eight extra Meridians theory to birth physiology can help to explain these modern information, and thus make them more acceptable.

• Journal of Environmental Science International
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• v.16 no.2
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• pp.219-225
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• 2007

Endocrine disrupting compounds (EDC's) are chemicals that either mimic endogenous hormones interfering with pharmacokinetics or act by other mechanisms. Some endocrine disrupters were reported to be chemical substances that cause apoptosis in cells. A number of reports have indicated that 1,3-DCP, one of the EDC's may act as an endocrine disrupter and also has possible carcinogenic effects. 1,3-DCP, present in commercial protein hydrolysates used for human nutrition, are genotoxic and 1,3-dichloro-2-propanol induced tumors in rats. In the present study, it was investigated whether 1,3-DCP induces ROS generation and apotosis in A549 adenocarcinoma cells. Here we show that 1,3-DCP inhibits the growth of lung cancer cell lines and generates reactive oxygen species (ROS), a major cause of DNA damage and genetic instability, It was investigated that 1,3-DCP increases G1 phase cells after 12 hours, thereafter abruptly draws A549 cells to G0 state after 24 hours by flow cytometric analysis. 1,3-DCP induces p53 and $p21^{Cip1/WAF1}$ activation time- and dose-dependently by 24 hours, while the level $p21^{Cip1/WAF1}$ was decreased after 48 hours. These results suggest that 1,3-DCP, an EDC's generates ROS and regulates genes involved with cell cycle and apoptosis.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.7
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• pp.919-928
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• 2011

The pig exhibits true epitheliochorial placentation, where the fetal membrane maintains attachment throughout pregnancy but does not invade into the maternal uterine endometrium. Accordingly, the expression and function of cell adhesion molecules are very important for embryo implantation and the establishment of pregnancy. In our recent microarray analysis, we found that activated leukocyte cell adhesion molecule (ALCAM) was expressed in the uterine endometrium during pregnancy in pigs. To better understand the roles of ALCAM in the establishment and maintenance of pregnancy, we examined ALCAM expression in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that ALCAM was differentially expressed in the uterine endometrium during the estrous cycle and pregnancy, with the highest levels on D12 of pregnancy. ALCAM mRNA was localized to the luminal and glandular epithelial cells and to the trophectoderm of conceptuses during early pregnancy. The steroid hormones estrogen and progesterone had no effect on ALCAM expression in an endometrial explant culture study. Further, we found that ALCAM expression in the uterine endometrium from gilts with somatic cell nuclear transfer-derived embryos was not different from that in gilts with embryos from natural mating. ALCAM was expressed in a pregnancy stage- and cell type-specific manner in the uterine endometrium and conceptuses during pregnancy. These findings suggest that ALCAM may play a role in the establishment of pregnancy. Further analysis of ALCAM will provide insight into the implantation process and establishment of pregnancy in pigs.

• Journal of Animal Science and Technology
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• v.59 no.5
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• pp.12.1-12.7
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• 2017

Background: Despite the widespread use of dexamethasone in veterinary and human medicine, it is reported to cause some severe pregnancy related side effects like abortion in some animals. The mechanism of the response is not clear but seems to be related to interspecies and/or breed difference in response which may involve alterations in the concentrations of some reproductive hormones. Methods: Twenty Sahel goats comprising 18 does and 2 bucks were used for this study. Pregnancies were achieved by natural mating after synchronization. Repeated dexamethasone injections were given at 0.25 mg/kg body weight. Blood samples were collected biweekly for hormonal assay. Uterine biopsies were harvested at days 28 and day 78 of gestation through caesarean section for immunohistochemical analysis using 3 pregnant does randomly selected from each group at each instant. Data were expressed as Means ${\pm}$ Standard Deviations and analyzed using statistical soft ware package, GraphPad Instant, version 3.0 (2003) and progesterone receptor (PR) were scored semi-quantitatively. Results: Dexamethasone treatments had no significant (p > 0.05) effect on progesterone and estrogen concentrations in pregnant Sahel goats but up regulated PR from 2+ to 3+ in second trimester. Conclusion: As dexamethasone adverse effect on placenta is an established fact, the lack of effect on progesterone level in this study may be due to the fact that unlike other species whose progesterone production during pregnancy is placenta - dependent, in goats is corpus luteum - dependent. Consequently dexamethasone adverse effect on placenta reported in literatures did not influence progesterone levels during pregnancy in Sahel goat. The up regulation of progesterone receptor (PR) in Sahel goat gravid uterus is a beneficial effects and that dexamethasone can safely be used in corpus luteum - dependent progesterone secreting pregnant animal species like Sahel goat and camel. Therefore source of progesterone secretions during pregnancy should be considered in clinical application of dexamethasone in pregnancy.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.146-153
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• 2018

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

• Development and Reproduction
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• v.9 no.2
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• pp.85-93
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• 2005

Estrogens are known as the steroid hormones and essential regulators of developments, differentiations, and fertility in animals including humans. Recently, classic focus on estrogens which are considered as female hormone is changing in the whole field of reproductive endocrinology. Especially, interest in estrogen functions in male reproduction is increasing more and more, as numerous studies about the endocrine disruptors, interrupting the endocrine system, are being carried out. To understand exactly the function of estrogen in a male reproductive system, a summary for estrogen receptors upon developmental distributions in testis will be useful. In addition to the regulatory roles of estrogen in male, unexpected exposure to exogenous estrogens causes defects of differentiation of male reproductive system and an injury of spermatogenesis. Also, this review highlights the indicator of exogenous estrogens to perturb male fertility. These approaches would give tile practical information about estrogen roles in male development and reproduction.

• Development and Reproduction
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• v.15 no.2
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• pp.167-172
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• 2011

To monitor the reproductive patterns of endangered common chimpanzee including annual reproductive cycle, amenorrhea, breeding season, and pregnancy diagnosis, Time-Resolved Fluorescence Immuno Assay (TR-FIA) was used to trace MRH (estradiol, progesterone, testosterone) and human chorionic gonadotropin (HCG). In result of this research, age was not the important factor in determining the reproduction capability in common chimpanzee; it was rather greatly influenced by the combination of various factors such as individual's fertilizing ability, presence of obstetrical diseases, the pattern of mating behavior, and the mental communication under the introduction of a mating partner. This research will play an important role in operating conservation project for common chimpanzees and can be extended also for shedding new light on understanding human menopause and obstetrical diseases.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.1-10
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• 1996

Factory-style plant production systems of various kinds are the final goal of greenhouse production systems. These systems facilitate planning for constant productivity per unit area and labor under various outside weather conditions, although energy consumption is intensive. Physical environmental control in combination with biological control can replace the use of agricultural chemicals such as insecticides, herbicides and hormones to regulate plants. In this way, closed systems which do not use such agricultural chemicals are ideal for environmental conservation for the future. Nutrient components in plants can be regulafied by physical environmental control including nutrient solution control in hydroponics. Therefore, specific contents of nutrients for particular plants can be listed on the container and be used as the basis of customer choice in the future. Plant production systems can be classified into three types based on the type of lighting: natural lighting, supplemental lighting and completely artificial lighting (Plant Factory). The amount of energy consumption increases in this order, although the degree of weather effects is in the reverse order. In the addition to lighting, factory-style plant production systems consist of mechanized and automated systems for transplanting, environmental control, hydroponics, transporting within the facility, and harvesting. Space farming and development of pharmaceutical in bio-reactors are other applications of these types of plant production systems. Various kinds of state-of-art factory-style plant production systems are discussed in the present paper. These systems are, in general, rather sophisticated and mechaized, and energy consumption is intensive. Factory-style plant production is the final goal of greenhouse production systems and the possibilities for the future are infinte but not clear.