• Title/Summary/Keyword: Natural Science

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Spectrofluorimetric Determination of Bisphosphonates in Biological Sample with a Fluorescent Chemosensor, NadDPA-2Zn2+

  • Jeong, Yun-Seong;Kim, Soon-Young;Jin, Geun-Woo;An, Song-Hie;Lee, Jae-Han;Jeong, A-Reum;Chio, Yeon-Soon;Hong, Jong-In;Park, Jong-Sang
    • Bulletin of the Korean Chemical Society
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    • v.31 no.9
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    • pp.2561-2564
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    • 2010

  • The accurate determination of bisphosphonate levels in bone and biological fluids is important in both clinical and pharmacological/toxicological studies; however, the quantitative analysis of the bisphosphonate is difficult because its concentration is quite low in most of biological sample. A novel fluorescent chemosensor (FCS)-based measurement method of bisphosphaonate levels using Naphta-diDPA-$2Zn^{2+}$ (NadDPA-$2Zn^{2+}$, DPA = dipycolylamine), an excellent FCS previously used for detecting PPi, was developed. By the FCS method, the concentration of bisphosphonates having no fluorophores can be determined analyzed with sufficient sensitivity. The results of this study indicate that the FCS-based measurement can be a useful method to analyze bisphosphonates in biological samples.

  • https://doi.org/10.5012/bkcs.2010.31.9.2561 인용 PDF KSCI

Molecular Cloning and mRNA Expression a Glutathione S-Transferase cDNA from the Spider, Araneus ventricosus

  • Shin, Geun Ho;Kim, Hyung Suk;Kwon, Dong Wook;Lee, Jin Young;Byeon, Gyeong Min;Sohn, Hung Dae;Jin, Byung Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.65-71
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    • 2004

  • A fat body-specific glutathione S-transferase cDMA was cloned from the spider, Araneus ventricosus. The cDNA encoding A. ventricosus glutathione S-transferase (AvGST) is 645 base pairs long with an open reading frame of 215 amino acid residues with a calculated molecular weight of approximately 24 kDa. Northern blot analysis showed the tissue-specifically expression of AvGST in the A. ventricosus fat body.

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