• BMB Reports
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• v.28 no.2
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• pp.129-137
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• 1995

The ryanodine-receptor $Ca^{2+}$ release channel protein in the sarcoplasmic reticulum membrane of rabbit skeletal muscle plays an important role in muscle exitation-contraction (E-C) coupling. Various types of detergents were tested, including Chaps, cholate, octylglucoside, Zwittergents, Mega-9, Lubrol PX, and Triton X-100 for solubilization of this protein. Among these, Chaps and Triton X-100 were found to optionally solubilize the channel complex. Optimum conditions for this solubilization were pH 7.4 with a salt concentration of 1 M. The addition of phospholipid in the solubilization step helped in stabilizing the protein. The purification of the receptor was performed using sucrose density gradient centrifugation. Various methods [dilution, freeze-thaw, adsorption (Biobeads), and dialysis] were investigated to incorporate the Chaps-solubilized and purified $Ca^{2+}$ release channel protein into liposomes made from different types of phospholipids. Of these, a combined method consisting of a dialysis, freeze-thaw and sonication steps yielded the best results. Reconstituted vesicles produced by this method with 95% phosphatidylcholine (from soybean extract) had good function.

• Economic and Environmental Geology
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• v.27 no.6
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• pp.579-592
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• 1994

A case study is presented where a fluvial system is modeled in three dimensions and compared to data gathered from a study of the Arkansas River. The data is unique in that it documents changes that affected a straight channel that was excavated within the river by the U.S. Army Corps of Engineers. Excavation plan maps and sequential aerial photographs show that the channel underwent massive deposition and channel migration as it returned to a more natural, meandering path. These records illustrate that stability of fluvial system can be disrupted either by catastrophic events such as floods or by subtle events such as the altering of a stream's equilibrium base level or sediment load. SEDSIM, Stanford's Sedimentary Basin Simulation Model, is modified and used to model the Arkansas River and the geologic processes that changed in response to changing hydraulic and geologic parameters resulting from the excavation of the channel. Geologic parameters such as fluid and sediment discharge, velocity, transport capacity, and sediment load are input into the model. These parameters regulate the frequency distribution and sizes of sediment grains that are eroded, transported and deposited. The experiments compare favorably with field data, recreating similar patterns of fluid flow and sedimentation. Therefore, simulations provide insight for understanding and spatial distribution of sediment bodies in fluvial deposits and the internal sedimentary structure of fluvial reservoirs. These techniques of graphic simulation can be contributed to support the development of the new design criteria compatible with natural stream processes, espacially drainage problem to minimize environmental disruption.

• Journal of Mechanical Science and Technology
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• v.20 no.12
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• pp.2043-2051
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• 2006

This paper considered identification of vibration characteristics of flexible structure with vector channel periodic lattice filter. We present an algorithm for AR coefficients for the vector-channel lattice filters, and characteristic equation and transfer function are derived from these coefficients. Vibration lattice filter is then constructed from the vector channel lattice filter, and performance of this vibration filter is tested with a test signal which is a combination of many sine waves to compare the performance of scalar and vector channel lattice. Also it is applied to the cantilever data to identify properties of the system, such as natural frequencies and damping ratios, to show its performance.

• Journal of Korean Society of Water and Wastewater
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• v.26 no.4
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• pp.503-512
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• 2012

In Yongwon channel, its natural flow of seawater is blocked by the construction of Busan New Port including the container berth. The channel was transformed into a narrow and long one, where it is possible that ships are only allowed to pass through the north-side channel of Gyeonmado located at the point of river mouth to Songjeongcheon. In addition, Yongwon channel is approximately 100 m wide on average and 3,600 m long, and has the highest slenderness ratio (length/width = 36). So it is considered that the changes in the terrain characteristics of Yongwon channel is likely to alter the circulation of sea water, thereby changing its water quality. In this study, the bottom sediment were collected from the 48 points of the Yongwon channel and the long-term leaching characteristics were analyzed. Thus, changes in sediment and water quality were analyzed through the sediment release test to investigate the degree of contamination. The sediment release from the inside region was higher than the outer region, which is due to the pollutant that comes form the downtown area. The results of this study can be used to predict the water quality in the future and prepare the economically optimized countermeasures to improve the water quality of Yongwon channel, Busan New Port.

• Journal of Korean Society of Water and Wastewater
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• v.27 no.2
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• pp.185-196
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• 2013

In Yongwon channel, its natural flow of seawater is blocked by the construction of Busan Newport including the container berth. The channel was transformed into a narrow and long one, where it is possible that ships are only allowed to pass through the north-side channel of Gyeonmado located at the point of river mouth to Songjeongcheon. In addition, Yongwon channel is approximately 100 m wide on average and 3,600 m long, which has the highest slenderness ratio (length/width=36). So it is considered that the changes in the terrain characteristics of Yongwon channel is likely to alter the circulation of sea water, thereby changing its water quality. In this study, the seasonal change and the spatial variation of the water quality in Yongwon channel was analyzed for the effect of land pollution using the measurement data. The mass balance calculation method is used to analyze the water pollution resulting from sediment pollutants. This result shows that the improvement of the water quality in Yongwon channel can be obtained from the sewer pipe modification and the environmental dredging.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.60 no.2
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• pp.252-256
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• 2011

We analyze the channel capacity with considering the effect of the loading conditions of indoor PLC networks on channel state information. We consider various numbers of load for two kinds of the networks with regular length branches and a deployed network of indoor PLC. For calculating the channel capacity degradation, two noise scenarios and impedances are considered. From the simulation results, we suggest the robust regression lines for modeling the channel capacity degradation. In the cases of 0 $\Omega$ and $Z_0$ loads, natural log and linear function curve show the best goodness of fit, respectively. For the deployed indoor PLC network with 0 $\Omega$ loads, compared with the networks with regular length branches, the goodness of fit decreases by the amount of 0.12 and 0.15 for low noise and high noise scenarios, respectively. Using the regression lines, we can estimate the channel capacity degradation without measurement.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.1661-1665
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• 2006

In a natural river, cross sections of a channel vary according to inner or outer parts of meandering. Generally, depth of outer parts is deeper than that of inner parts. This kind of cross section change by meandering can be demonstrated by Beta distribution. The objects of this research is a 3D simulation of primary and secondary flow in the meandering natural channel. FLOW-3D program, a numerical model using CFD technique, and LES method was used for this research. 3D simulations were conducted in the channels having Beta distribution cross sections which have beds of mortar, gravel and vegetation. Two types of water stages and discharge were applied to each channel. In this research, primary flows are located in the outer parts of a top of bend and secondary flows rotate in the bottom on outer parts.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.43 no.4 s.346
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• pp.23-30
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• 2006

This paper describes the short-channel effect(SCE), corner effect of nano-scale MuGFETs(Multiple-Gate FETs) by three-dimensional simulation. We can extract the equivalent gate number of MuGFETs(Double-gate=2, Tri-gate=3, Pi-gate=3.14, Omega-gate=3.4, GAA=4) by threshold voltage model. Using the extracted gate number(n) we can calculate the natural length for each gate devices. We established a scaling theory for MuGFETs, which gives a optimization to avoid short channel effects for the device structure(silicon thickness, gate oxide thickness). It is observed that the comer effects decrease with the reduction of doping concentration and gate oxide thickness when the radius of curvature is larger than 17 % of the channel width.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 1996.12a
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• pp.70-70
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• 1996

The cytotoxicological effect of Bt 1-endotoxin, well-known as a bioinsecticide, was investigated on ion channel of insect cell lines. This study attempted to evaluted the specificity by simple experiment to measure the cell swelling using lepidopteran cell lines in isotonic solution containing only one cation. Cell swelling was stimulated in KCI-sucrose isotonic solution as well as TC-100 media containg in solubilized crystal 5-endotoxin. It suggested that the cell swelling by Bt toxin have a relation to K+ channel. The cell swelling may be due to the stimulation K+ influx and simultaneously the penetration of H2O induced by Bt toxin, because the stimulation of swelling was observed with the solubilized toxin in KCI-sucrose isotonic solution, but not in sucrose isotonic solution. Moreover the specific K+ channel blocker, such as 4-arnjnopyrimidine(4-AP) and ouabain, showed the significant effect on the cell swelling induced by Bt toxin. The increasement of the cell swelling induced by 4-AP suggested to be caused by the block of K+ efflux through K+ leak channels. The inhibition of cell swelling by ouabain, which is the well-known inhibitor of Na+, K+-ATPase, suggested to be due to decreasement of K+ influx following diminishment of Na+, K+-ATPase activities.

• Solar Energy
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• v.19 no.3
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• pp.29-41
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• 1999

Three-dimensional natural convective heat transfer in a vertical channel with a protruding single module was investigated experimentally. The particular interest was in the removal of thermal energy from the module by convective heat transfer. Hence radiative and conductive heat losses were estimated by using thermocouples and heat flux sensor respectively. The flow fields in the channel were visualized by means of a smoke-method. Also, local temperatures were measured by thermocouples inside the channel, along the vertical wall and module surface. It is found that convective heat transfer was promoted at the lower comer of the module and was decreased at the upper comer due to a recirculation zone. A general correlation of the critical channel ratios was found as a function of Rayleigh number. For the range of $8.28{\times}10^3<Ra^*_c<3.48{\times}10^6$, a useful correlation for the mean Nusselt number was proposed as a function of modified channel Rayleigh number.