• 대한핵의학회:학술대회논문집
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• 대한핵의학회 1988년도 제27차 춘계학술대회
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• pp.140.1-140.1
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• 1988

• The Korean Journal of Physiology and Pharmacology
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• 제21권6호
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• pp.667-674
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• 2017

Angiotensin II (Ang II) is metabolized from N-terminal by aminopeptidases and from C-terminal by Ang converting enzyme (ACE) to generate several truncated angiotensin peptides (Angs). The truncated Angs have different biological effects but it remains unknown whether Ang-(4-8) is an active peptide. The present study was to investigate the effects of Ang-(4-8) on hemodynamics and atrial natriuretic peptide (ANP) secretion using isolated beating rat atria. Atrial stretch caused increases in atrial contractility by 60% and in ANP secretion by 70%. Ang-(4-8) (0.01, 0.1, and $1{\mu}M$) suppressed high stretch-induced ANP secretion in a dose-dependent manner. Ang-(4-8) ($0.1{\mu}M$)-induced suppression of ANP secretion was attenuated by the pretreatment with an antagonist of Ang type 1 receptor ($AT_1R$) but not by an antagonist of $AT_2R$ or $AT_4R$. Ang-(4-8)-induced suppression of ANP secretion was attenuated by the pretreatment with inhibitor of phospholipase (PLC), inositol triphosphate ($IP_3$) receptor, or nonspecific protein kinase C (PKC). The potency of Ang-(4-8) to inhibit ANP secretion was similar to Ang II. However, Ang-(4-8) $10{\mu}M$ caused an increased mean arterial pressure which was similar to that by 1 nM Ang II. Therefore, we suggest that Ang-(4-8) suppresses high stretch-induced ANP secretion through the $AT_1R$ and $PLC/IP_3/PKC$ pathway. Ang-(4-8) is a biologically active peptide which functions as an inhibition mechanism of ANP secretion and an increment of blood pressure.

• 대한수의학회지
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• 제39권5호
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• pp.930-937
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• 1999

Atrial natriuretic peptide(ANP) is a hormone with potent natriuretic, diuretic and relaxing properties on vascular smooth muscle. Specific chemical modulator in response for the ANP secretion has not been found yet. Therefore, we have investigated the role of $Ca^{2+}$ responsible for the regulation of ANP induced by protein kinase C(PKC) on mechanically stretch-induced ANP secretion in the rat atria. The results obtained were as follows ; 1. ANP secretion and ANP concentration were increased to more in $Ca^{2+}$-free buffer than in the Kreb-Henseleit buffer on mechanically stretch-induced ANP secretion(p < 0.05), but extracellular fluid translocation(ECF) was not significant. Phorbol 12-myristate 13-acetate(PMA, $10^{-7}M$) induced ANP secretion and ANP concentration in $Ca^{2+}$-free buffer shown to more accentuate on mechanically stretch-induced ANP secretion than in the $Ca^{2+}$-free buffer(p < 0.05), but ECF translocation was not significant. 2. In the presence of ryanodine($3{\times}10^{-6}M$), PMA($10^{-7}M$) induced ANP secretion and ANP concentration in the Kreb-Henseleit buffer were shown to more increase on mechanically stretch-induced ANP secretion than in the ryanodine($3{\times}10^{-6}M$) with the Kreb-Henseleit buffer(p < 0.05), but ECF translocation was not significant. 3. In the presence of ryanodine($3{\times}10^{-6}M$), PMA($10^{-7}M$) induced ANP secretion and ANP concentration in the $Ca^{2+}$-free buffer was shown to more increase on mechanically stretch-induced ANP secretion than in the ryanodine($3{\times}10^{-6}M$) with the $Ca^{2+}$-free buffer on mechanically induced ANP secretion(p < 0.05), but ECF translocation was not significant. The results suggest that PKC-induced ANP secretion may not be related to the change of $Ca^{2+}$ on mechanically induced ANP secretion in the rat atria.

• Journal of Acupuncture Research
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• 제20권6호
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• pp.1-12
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• 2003

Objective : The aim of this experiments was to investigate the effects of Kyongo Puryu(originated from ${\ll}$Classic on Difficulty${\gg}$), Kyongo Um-gok(originated from ${\ll}$Ling Shu${\gg}$) acupuncture on the Blood Pressure, Cardiomegalic index, and plasma levels of atrial natriuretic peptide in Hypertensive RAT induced by 2K1C. Methods : The effects of Kyongo(L8) Puryu(K7), Kyongo(L8) Um-gok(K10) acupuncture on Blood Pressure in Hypertensive RAT induced by Two Kidney One Clip(2K1C). Results : I. Blood Pressure was decreased significantly after acupuncture of L8 K7. II. Cardiomegalic index decreased significantly after acupuncture of L8 Puryu, L8 K10. IlI. Plasma levels of atrial natriuretic peptide was increased significantly after acupuncture of L8 K7.

• Journal of Korean Biological Nursing Science
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• 제18권4호
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• pp.247-256
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• 2016

Purpose: This study aimed to examine the relationships between the N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels with the sociodemographic and clinical factors, self-care behaviors, and the physical symptom experiences in patients with heart failure. Methods: This cross-sectional study utilized a convenience sample of adult outpatients and inpatients who attended a cardiology department in a tertiary hospital in Seoul, Korea. The data from 154 patients with heart failure were collected using a questionnaire, and their clinical data were extracted from their electronic medical records. Results: Compared with the patients with high NT-proBNP levels, those with low NT-proBNP levels had significantly lower physical symptom experiences scores. Patients with low- and mid-NT-proBNP levels were more likely to be employed compared with those with high NT-proBNP levels. Patients with low NT-proBNP levels had higher left ventricular ejection fractions, and were less likely to have arrhythmias and comorbidities. Conclusion: The results from this study showed that patients with more severe heart failure had higher physical symptom experiences scores; hence, individualizing treatment approaches based on heart failure severity is necessary.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.158-164
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• 2014

Substance P and neuropeptide Y were discovered as early diagnostic biomarkers of acute myocardial infarction in Korean patients and confirmed using enzyme-linked immunosorbent assay (ELISA). We screened 12 peptides from the sera of Korean acute myocardial infarction (AMI) patients and detected 3 peptides (neuropeptide Y, substance P, and N-terminal pro-B-type natriuretic peptide) to be elevated from patients' sera by liquid chromatography mass/mass spectrometry. The elevated concentration of 3 peptides was confirmed by ELISA. The screening results revealed the substance P, neuropeptide Y, and pro-B-type natriuretic peptide (47-76) concentrations were higher in patients' sera than in healthy controls. The sensitivity and specificity of substance P for AMI diagnostic marker were 80% and 83%, respectively, and those of neuropeptide Y were 87% and 90%, respectively compared to healthy controls. These results suggest that substance P and neuropeptide Y could be used as early diagnostic biomarkers in patients with AMI.

• 한국동물학회지
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• 제35권1호
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• pp.8-16
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• 1992

포유류의 심방에 주로 존재하여 체액 및 전해질 대사의 조절과 혈압 조절에 관여하는 atrial natriuretic peptide(ANP)가 담수어류인 미꾸라지(Misgumus mizolepis G.)의 심장 및 기타조직에도 존재하는지 알아보고 이들의 분자적 특성 및 온도차에 의한 함량변동을 조사하였다. 미꾸라지의 심장과 동맥구의 추출액은 모두 희석농도에 따라 표준곡선에 평행하는 ANP immunoreactivity를 나타냈다. 미꾸라지의 심장과 동맥구 모두에서 저분자량 및 고분자량의 immunoreactive ANP(irANP)가 관찰되었다. 한편 미꾸라지의 심장내 irANP함량은 61.56 $\pm$ 5.08 pg/mg이었으며, 심장외 조직인 동맥구는 60.59 $\pm$ 5.04 pg/mg으로 거의 동량의 irANP를 함유하였다. 미꾸라지를 저온상태에 적응시킨 경우에는 심장보다도 동맥구에서 더 유의한 irANP의 함량변동을 보였다(p < 0.01), 미꾸라지의 심장 및 동맥구에 존재하는 irANP는 주로 고분자량의 Pro-ANP가 관찰되므로써 미꾸라지의 심장과 동맥구 모두가 irANP를 조직내에서 합성할 것임을 시사하였다. 아울러 온도는 미꾸라지의 경우에서 irANP의 함량변동에 영향을 주는 요인임을 알 수 있었다.

• The Korean Journal of Physiology
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• 제23권1호
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• pp.51-66
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• 1989

Mammalian cardiocytes secrete atrial natriuretic peptides (ANPs) into plasma, which cause marked natriuresis, diuresis, vasorelaxation and inhibition of hormone secretions. Aging influences the ability of the kidney both to conserve and to excrete sodium; i.e., in old animals, the excretory capacity of sodium is reduced and the time required to excrete sodium load is prolonged. Therefore, it is possible that animals differing in ages may respond differently to ANP. In the present study, we determined whether the renal, hormonal and vascular effects of ANP may be influenced by aging in conscious rabbits. The plasma renin concentration decreased with aging but plasma ANP concentration was significantly lower only in 24-month-old rabbits. Plasma aldosterone concentration and atrial ANP content did not change by aging. In 1-month-old rabbits, ANP (atriopeptin III, 3 ug/kg) administered intravenously caused hypotension and decreased in plasma renin and aldosterone concentrations, but did not cause diuresis and natriuresis. In 2 to 5 month-old rabbits, ANP caused hypotension, decreases in Plasma renin and aldosterone concentrations and marked renal effects. However, in 24-month-old rabbits, all the above effects of ANP was blunted. With hydration of physiological saline at a rate of 15 ml/kg/h for 2hr, urine volume and glomerular filtration rate did not change but the electrolyte excretion as well as fractional excretion of sodium significantly increased. The plasma concentrations of active renin and aldosterone were decreased but plasma inactive renin and ANP concentrations were increased. The changes in renal function and plasma level of hormone showed no differences in different ages. These results suggest that the peripheral vascular receptors to ANP may develop earlier than those in the kidney, and the attenuated vascular and renal responses to ANP in the old age may be due to age-related modifications in renal function and blood vessel.

• Tuberculosis and Respiratory Diseases
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• 제62권5호
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• pp.389-397
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• 2007

연구배경: B-type natriuretic peptide(BNP)는 심인성 및 다른 쇼크 상태를 포함하는 심혈관 질환에서 사망을 예측하는 좋은 인자 중의 하나로 알려져 있다. 그러나, 급성호흡곤란증후군환자에서 이런 관계가 잘 알려져 있지 않는 바, 저자들은 BNP가 급성호흡곤란증후군 환자에서 사망을 예측할 수 있는 지를 연구하였다. 방 법: 본 연구는 전향적 관찰로 시행되었다. 급성호흡곤란증후군으로 진단된 환자들에게 심초음파 검사를 시행한 후, 좌심실 구획률이 50% 미만이거나 확장성 심부전 양상을 보인 환자들을 제외하였다. 2003년 12월부터 2006년 2월까지 총 47명의 환자가 채택되었다. 등록된 환자들은 24시간 내로 BNP를 포함한 여러 검사실 수치를 얻었으며, APACHE(Acute Physiology and Chronic Health Evaluation) II 점수를 구하였다. 결 과: BNP와 APACHE II 점수 평균값은 생존자군과 사망자군에서 유의한 차의를 보였다(BNP: $219.5{\pm}57.7pg/mL$ vs $492.3{\pm}88.8pg/mL$; p=0.013, APACHE II 점수: $17.4{\pm}1.6$ vs $23.1{\pm}1.3$, p=0.009). BNP 는 혈중 크레아티닌 수치와 양의 상관관계를 보였으나(r=0.374, p=0.01), 좌심실 구획률과는 유의한 관계가 없었다. Receiver operating characteristic 곡선상, BNP 수치를 585 pg/mL로 잡았을 때 사망을 예측하는데 있어서 94%의 특이도를 보였으며, APACHE II 점수의 경우에는 15.5를 기준으로 하였을 때 87%의 민감도를 보였다. 이 두 요소를 결합하여 '아파치II 점수+$11{\times}logBNP$' 수치를 계산하여 기준점을 46.14로 했을 경우, 사망 예측에 있어서 민감도 63%, 특이도 82%의 결과를 얻을 수 있었다. 결 론: 좌심실 기능 부전을 보이지 않는 급성호흡곤란증후군 환자에서 BNP 수치는 생존자군과 사망자군에서 유의한 차이를 보였으며 사망을 예측할 수 있었다. 향후 급성호흡곤란증후군 환자에 있어서 BNP와 관련된 연구가 더 필요하다고 생각된다.

• 대한임상검사과학회지
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• 제48권4호
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• pp.394-400
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• 2016

B-type natriuretic peptide (BNP)와 심초음파는 심부전 진단과 예후를 판단하는 지표로써 수축 기능이상에 많은 연구를 했으나, 본 연구에서는 BNP 결과와 심초음파 이완 기능을 나타내는 항목의 상관관계를 알아보고자 한다. BNP 검사와 심초음파를 실시한 188명을 대상으로 BNP 결과에 따라 5그룹으로 분류하고, 심초음파의 좌심실 구혈률, 좌심방 크기, E velocity, A velocity, Deceleration time, E/A ratio, E', A', S', E/E'와의 상관관계를 조사하고, 그 중 심부전을 진단 받은 67명을 Relaxation abnormality (Grade I), Pseudonormalization (Grade II), Restrictive physiology (Grade III)로 등급을 분류하고 BNP 결과와 심초음파 항목을 비교한 결과 BNP 수치가 상승할수록 심초음파 항목 중 좌심실 구혈률, 좌심방 크기, E/A ratio, Deceleration time, A', S', E/E'와 유의한 상관관계가 있었다. 그룹간의 비교에서 E/E' 항목이 가장 좋은 유의성을 보여주었다. 심부전 환자의 등급 분류에 따른 BNP 결과는 $623.0{\pm}459.7pg/mL$, $1013.2{\pm}1155.1pg/mL$, $1693.4{\pm}1544.0pg/mL$로 유의성이 있었으며(p<0.01), 등급이 올라갈수록 심초음파 항목 중 좌심실 구혈률, 좌심방 크기, E velocity, A velocity, E/A ratio, Deceleration time, A', E/E' 와 상관관계가 있었다(p<0.001). BNP 결과가 상승할수록 심초음파 이완기 항목과 유의성이 있었고, 심초음파에서 Restrictive physiology 그룹의 BNP 결과가 가장 높았다.