• 식물분류학회지
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• 제45권3호
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• pp.266-271
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• 2015

통영시 미륵산 용화사 근처에서 보리수나무과에 속하는 미기록 분류군인 통영볼레나무(Elaeagnus pungens Thunb.)가 발견되었다. 이 분류군은 중국과 일본 원산으로, 북아메리카에는 도입되어 분포하고 있다. 근연 분류군인 보리장나무(Elaeagnus glabra)와 비교하여 경침이 존재하며, 엽연은 파상형으로 뒤로 젖혀지고, 잎 뒷몃은 은백색 인모에 드물게 갈색 인모가 있으며, 꽃받침통은 종형의 형태를 가진다는 점에서 뚜렷이 구분된다. 국명은 채집지의 명칭을 따서 '통영볼레나무'로 명명하였으며, 형태적 특징의 기재, 생태사진, 도해, 표본사진 및 검색표를 제시하였다.

• KSBB Journal
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• 제9권1호
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• pp.55-62
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• 1994

Aspergillus niger 균체로부터 황산암모늄 분별침전, 이온교환 크로마토그래피, 한외여과, 소수성 크로마토그래피 과정을 거쳐 glucose oxidase( EC 1.1. 3.4)를 순수 정제하였다. 최종 정제과정인 소수성 크로마토그래퍼에 의해 소수성은 다르나 glucose oxidase의 활성을 갖는 A, B fraction이 얻어졌고, 그의 비활성도는 각각 2,191, 1,273units/mg이었다. A와 B는 분자량 78,000의 당단백질임이 확인되었으나, HPLC 겔 여과로 측정한 분자량, 최대 흡수 파장, 등전점 등에 차이를 보였다. 효소 A는 $\beta$-D-glucose를 특이적으로 산화하였고, 촉매 활성에 대한 최적 온도 는 $30^{\circ}C$, 최적 pH는 3.5이었으며, SDS에 대하여 비교적 강한 저항성을 나타내었고, 촉매 기능은 10mM $Hg^{2+}$에 의해 급격히 억제되었다. 화학 수식 실험 결과 cysteine/ cystine중의 SH기가 glucose oxidase의 활성에 관여하고 있음이 시사되었다.

• Parasites, Hosts and Diseases
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• 제53권1호
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• pp.65-75
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• 2015

Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation of substrate proteins. A cDNA clone of C. sinensis was predicted to encode a polypeptide homologous to parkin (CsParkin) including 5 domains (Ubl, RING0, RING1, IBR, and RING2). The cysteine and histidine residues binding to $Zn^{2+}$ were all conserved and participated in formation of tertiary structural RINGs. Conserved residues were also an E2-binding site in RING1 domain and a catalytic cysteine residue in the RING2 domain. Native CsParkin was determined to have an estimated molecular weight of 45.7 kDa from C. sinensis adults by immunoblotting. CsParkin revealed E3-ubiquitin ligase activity and higher expression in metacercariae than in adults. CsParkin was localized in the locomotive and male reproductive organs of C. sinensis adults, and extensively in metacercariae. Parkin has been found to participate in regulating mitochondrial function and energy metabolism in mammalian cells. From these results, it is suggested that CsParkin play roles in energy metabolism of the locomotive organs, and possibly in protein metabolism of the reproductive organs of C. sinensis.

• BMB Reports
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• 제36권4호
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• pp.409-416
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• 2003

The isoform 1 of cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) from Paenibacillus sp. A11 was purified by a preparative gel electrophoresis. The importance of histidine, tryptophan, tyrosine, and carboxylic amino acids for isoform 1 activity is suggested by the modification of the isoform 1 with various group-specific reagents. Activity loss, when incubated with diethylpyrocarbonate (DEP), a histidine modifying reagent, could be protected by adding 25 mM methyl-$\beta$-cyclodextrin substrate prior to the modification. Inactivation kinetics of isoform 1 with DEP resulted in second-order rate constants ($k_{inactivation}$) of $29.5\;M^{-1}s^{-1}$. The specificity of the DEP-modified reaction for the histidine residue was shown by the correlation between the loss of isoform activity and the increase in the absorbance at 246 nm of N-carbethoxyhistidine. The number of histidines that were modified by DEP in the absence and presence of a protective substrate was estimated from the increase in the absorbance using a specific extinction coefficient of N-carbethoxyhistidine of $3,200\;M^{-1}cm^{-1}$. It was discovered that methyl-$\beta$-CD protected per mole of isoform 1, two histidine residues from the modification by DEP. To localize essential histidines, the native, the DEP-modified, and the protected forms of isoform 1 were digested by trypsin. The resulting peptides were separated by HPLC. The peptides of interest were those with $R_t$ 11.34 and 40.93 min. The molecular masses of the two peptides were 5,732 and 2,540 daltons, respectively. When the data from the peptide analysis were checked with the sequence of CGTase, then His-140 and His-327 were identified as essential histidines in the active site of isoform 1.

• 한국환경복원기술학회지
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• 제15권3호
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• pp.55-66
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• 2012

This study was carried out to investigate vegetation of Rhododendron mucronulatum habitat in Mt. Biseul to recommend basic information for synecological characteristics and management methods. The survey was carried out from May to July, 2011 and totally 46 vegetation data including 42 families 93 genera 108 species 16 varieties and 5 forma were collected and analyzed. Among the investigated 129 taxa, the numbers of rare plant resources were summarized as 19 taxa; The rare plants designated by Korea Forest Service, the specific plants by floristic region and the endemic plants were listed as 3 taxa, 15 taxa and 4 taxa, respectively. Rhododendron mucronulatum habitat of Mt. Biseul was classified into 3 plant communities (Carex lanceolata-Rhododendron mucronulatum community, Potentilla dickinsii-Selaginella rossii community, Carex lanceolata-Quercus mongolica community). Carex lanceolata-Rhododendron mucronulatum community was subdivided into Tripterygium regelii subunit and Miscanthus sinensis for. purpurascens subunit by human interference degree. And synecology, syndynamics, synchorology of these plant communities were identified, and Site-species ordination analysis by Principal Coordinate Analysis (PCoA) reflected that human impact, soil moisture condition were main ecological factors determining the distribution pattern of classified plant communities. Therefore these plant communities correspond to quite distinctive 4 habitat types : unstable-dry type=Miscanthus sinensis for. purpurascens subunit, unstable-moderate type=Tripterygium regelii subunit, stable-dry type=Potentilla dickinsii-Selaginella rossii community, stable-moderate type=Carex lanceolata-Quercus mongolica community. Finally, through the vegetational diagnosis, proper management methods such as a limit on the access of visitors, planting of native woody plants after removing unwanted vines or grass were suggested.

• 한국환경복원기술학회지
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• 제12권1호
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• pp.92-100
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• 2009

This study was carried out to investigate vegetation structure and soil properties of Angelica gigas population distributed in Jeombongsan, Bangtaesan, Odaesan, Gyebangsan and Jirisan. From August 2007 until September 2008, 5m${\times}$5m quadrat was established in native area of Angelica gigas in order to record a dominants and coverage, and soil factors at 20 sites. It was found that the altitude in the distributed areas for Angelica gigas population was 710m or more. Angelica gigas population was classified into Parasencio auriculata var. kamtschatical dominant population, Pseudostellaria palibiniana dominant population, Isodon excisus dominant population and typical dominant population. In the site of study, soil pH, electrical conductivity, soil organic matter, available phosphorous, and exchangeable potassium, exchangeable calcium, exchangeable magnesium, exchangeable natrium concentration and total nitrogen were ranged from 5.1~6.2, 0.1~0.79dS/m, 2.21~22.11%, 9.56~37.97mg/kg, 1.85~23.88cmol+/kg, 0.21~1.18cmol+/kg, 1.07~5.09cmol+/kg, 0.04~0.14cmol+/kg and 0.09~1.04% respectively. The Parasencio auriculata var. kamtschatical dominant population was found in area of high altitude and had high slope degree. But Pseudostellaria palibiniana dominant population was found in area of low altitude and low slope degree. Isodon excisus dominant population was found to have higher soil pH and exchangeable potassium concentration than the other dominant population.

• 한국환경복원기술학회지
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• 제17권3호
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• pp.13-33
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• 2014

The purpose of this study is to offer the raw data for establishing the management and restoration plan by objective surveying and analysing the flora distributed in Seoak District, Gyeongju National Park, Korea. The flora summarized as 411 taxa including 92 families, 285 genera, 363 species, 2 subspecies, 41 varieties and 5 forms. The rare plants designated by Korea Forest Service were 2 taxa including Exochorda serratifolia and Potentilla discolor. The Korean endemic plants were 3 taxa including Philadelphus schrenkii, Lespedeza maritima and Weigela subsessilis. The specific plants by floristic region were 16 taxa including Asplenium sarelii, Pyrrosia petiolosa, Vitex negundo var. incisa and so forth. The target plants adaptable to climate change were 2 taxa including Lespedeza maritima and Carpesium macrocephalum, and the plants with approval for delivering oversea were 4 taxa including Exochorda serratifolia, Glycine soja, Lespedeza maritima and Weigela subsessilis. The naturalized plants were 51 taxa including Phytolacca americana, Viola papilionacea, Lamium purpureum and so forth. The invasive alien plants were 3 taxa including Ambrosia artemisiifolia, Aster pilosus and Lactuca scariola. NI(Naturalized Index) was 12.4% of all 411 taxa of surveyed flora in this study and UI(Urbanized Index) was 15.9% of all 321 taxa of naturalized plants in Korea. Potentilla discolor in rare plant, Philadelphus schrenkii in endemic plant and Dictamnus dasycarpus in specific plant were established the conservation plan. Whereas, invasive alien plants such as Ambrosia artemisiifolia, Aster pilosus and Lactuca scariola should be removed as soon as possible. The damaged sites in Seoak District were divided between wildfire area and farmland. To restore a forest fire site, we will have to apply a natural renewal and community planting. In case of farmland, we will have to do ecological planting using native species and constrcut a forest wetland.

• Journal of Periodontal and Implant Science
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• 제50권3호
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• pp.197-206
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• 2020

Purpose: The aim of this study was to determine the impact of different compressive forces on deproteinized bovine bone mineral (DBBM) particles covered by native bilayer collagen membrane (NBCM) during alveolar ridge preservation (ARP) in the molar area, and to identify any histomorphometric and clinical differences according to the compressive force applied. Methods: Sockets were filled with DBBM after tooth extraction, and different compressive forces (30 N and 5 N, respectively) were applied to the graft material in the test (30 N) and control (5 N) groups. The DBBM in both groups was covered with NBCM in a double-layered fashion. A crossed horizontal mattress suture (hidden X) was then made. A core biopsy was performed using a trephine bur without flap elevation at the implant placement site for histomorphometric evaluations after 4 months. The change of the marginal bone level was measured using radiography. Results: Twelve patients completed the study. The histomorphometric analysis demonstrated that the mean ratios of the areas of new bone, residual graft material, and soft tissue and the implant stability quotient did not differ significantly between the groups (P>0.05). However, the mean size of the residual graft material showed a significant intergroup difference (P<0.05). Conclusions: The application of 2 compressive forces (5 N, 30 N) on particulate DBBM grafts during open-healing ARP in the posterior area led to comparable new bone formation, implant feasibility and peri-implant bone level.

• 한국환경생태학회지
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• 제21권2호
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• pp.168-175
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• 2007

탐방로의 효용 극대화를 위해서는 무엇보다도 탐방로 이용과 이용객의 특성을 이해하는 것이 필요하다. 이에 본 연구는 국립공원 탐방로의 효율적 유지관리를 위한 관리방안 마련을 위한 기초 연구로서 한라산국립공원 어리목 탐방로를 이용한 탐방객을 대상으로 이용행태와 보행시설물에 대한 이용자 인식을 파악할 목적으로 수행하였다. 이를 위해 대상지를 방문하고 귀가하는 탐방객 138명을 대상으로 응답자 직접기 입방식을 적용하여 설문조사를 실시하였다. 연구결과, 어리목에 설치된 7개의 보행 시설의 양적 질적 상태에 대한 응답은 대체적으로 보통 이상의 응답을 보여 시설 상태가 양호한 것으로 인식하고 있었다. 각 보행시설별 경관조화성, 자원보전성, 탐방편의성 평가에서는 목재데크 시설과 목재지주의 PP로프난간 시설과 같은 친자연적 소재를 사용한 시설물들이 긍정적으로 평가되었다. 반면에 철재와 같은 인공적 시설의 자연환경 지역에의 도입은 주변경관과의 조화를 저해하고 있는 것으로 인식하고 있어 향후 기존 철제 시설물에 대한 친자연적 소재로의 대체가 필요하다.

• BMB Reports
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• 제35권2호
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• pp.206-212
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• 2002

The NS2B-NS3(pro) polyprotein segment from the dengue virus serotype 2 strain 16681 was purified from overexpressing E. coli by metal chelate affinity chromatography and gel filtration. Enzymatic activity of the refolded NS2B-NS3(pro) protease complex was determined in vitro with dansyl-labeled peptide substrates, based upon native dengue virus type 2 cleavage sites. The 12mer substrate peptides and the cleavage products could be separated by reversed-phase HPLC, and were identified by UV and fluorescence detection. All of the peptide substrates (representing the DEN polyprotein junction sequences at the NS2A/NS2B, NS2B/NS3, NS3/NS4A and NS4B/NS5 sites) were cleaved by the recombinant protease NS2B-NS3(pro). No cleavage was observed with an enzymatically inactive S135A mutant of the NS3 protein, or with a modified substrate peptide of the NS3/NS4A polyprotein site that contained a K2093A substitution. Enzymatic activity was dependent on the salt concentration. A 50% decrease of activity was observed in the presence of 0.1M sodium chloride. Our results show that the NS3 protease activity of the refolded NS2B-NS3(pro) protein can be assayed in vitro with high specificity by using cleavage-junction derived peptide substrates.