• YAKHAK HOEJI
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• v.51 no.3
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• pp.199-205
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• 2007

Thermostable asparaginase was purified to homogeneity from mesophilic Strepfomyces lincolnensis M-20 by 30${\sim}$70% ammonium sulfate precipitation and asparagine-Sepharose CL 6B affinity column chromatography, The apparent molecular mass of L-asparaginase by SDS-PAGE was found to be 47 kDa, whereas by its mobility on Sephacryl S-300 column was around 180 kDa, indicating that the enzyme at the native stage acts as tetramer, The purified enzyme showed a single band on acrylamide gel electrophoresis. The optimum pH and temperature were pH 9.5 and 55${\circ}$C, respectively. Chemical modification experiments of purified asparagines implied the existence cystein residue located at or near active site. Purified asparaginase retained the 85% of the initial activity after incubation at 90${\circ}$C for 30 min. A correlation between themostability and resistance to proteolysis of commercial asparaginase and purified asparaginase from Strepfomyces lincolnensis M-20 was investigated. Purified thermostable asparaginase was resistant to trypsin and chymotrypsin treatment, while the commercial asparaginase was not themostable and was susceptible to proteolytic treatment with trypsin and chymotrypsin.

• English Language & Literature Teaching
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• v.15 no.4
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• pp.109-136
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• 2009

This study investigates English medium instruction (EMI) in an institution of higher education in Seoul, Korea to see whether this course creates cultural correction (reproduction of inequitable relations of power in EMI settings) or cultural continuity (opportunities for transporting students into a third space and enabling them to explore cultural diversity and to create new knowledge for themselves). A single site where EMI is carried out, a class on fairy tales and child education taught by a native English speaking professor, was chosen because it was hypothesized that the professor would display some of her unconscious dominant cultural orientation. The results of the study show that there more cases of cultural correction than there were of cultural continuity. Cases of cultural correction included lack of knowledge about the local context, fixing Korean classroom discourse as if it were American classroom discourse, and reproducing orientalism in the local educational setting. Cases of cultural continuity included using comparison to consider the cultural reality of the milieu, creating new knowledge for the local milieu, and learning as a dynamic ongoing process. Implications of this research are discussed including the important realization that EMI should be managed by subject specialists who are trained in language education and have knowledge of the students' needs and discourse in the L1 and in the local context.

• KIEAE Journal
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• v.14 no.5
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• pp.81-87
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• 2014

Pungsu is can be seen as an ecological architectural science regarding native natural environment of east asia. Even though the language used in Pungsu is different from that of ecological architecture, Pungsu considered the surrounding environment of the traditional house by various Pungsu method for making and keeping more healthy life and sustainable environment. The research is for finding out how the surrounding natural environment was considered with Pungsu, a traditional ecological architectural science in case of site selection and planning house by the confucian scholar in Joseon period. Myungjae Yunjung traditional house of Nonsan in the middle of korea is selected. He is one of the greatest confucian scholar in Joseon period. The study is processed as follows. Feature of mountain expressed as dragon and four important hills of the house are analyzed in chapter 2, Water environment is studied and the geomantic landscape are analyzed by shape theory called Hyung-guk-Lon in chapter 3, 4. Finally Pungsu applied in architectural space is analyzed in chapter 5.

• Journal of the Korean Institute of Landscape Architecture
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• v.23 no.3
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• pp.48-58
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• 1995

This study was conducted to investigate the plant community and to propose ecological planning methods for urban environmental forest on Gaepo neighborhood park, Gangnamgu in Seoul. Thirty-six plots that the size of each plot was 10$\times$10m(100\ulcorner) were set up and the vegetation analysis was carried out. Field survey was executed Apirl, 1994. The actual vegetation was consisted of Poplulus$\times$albaglandulosa community(73.03%), Robinia pseudoacacia community(8.30%) P. $\times$ albaglandulosa-R.pseudoacacia community(1.81%) and environmental forest (15.22%) in the survey site. According to mean importance values of each plot, survey area were divided into ten forest communities. As for the analysis of plant community structure which was classified into ten forest communities, the importance values, ecological diversity, number of species, number of individuals, basal area and crown coverage of urban environmental forest were relatively lower than those of the native community. In urban environmental forest of Gaepo neighborhood park, it showed problems for the planting method. So urban environmental forest should be constructed considering ecological characteristics based on above results.

• Journal of the Korean Institute of Landscape Architecture
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• v.15 no.3
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• pp.21-31
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• 1988

This study was executed to investigate the vegetation structure and to propose the administration planning of the Royal Ancestor's Shrine. Five sites sampled in the study area with the clumped sampling method. Pinus koraiensis community covered 27.3% and Quercus aliena community 61.5% of total forest area when considering the actual vegetation. This area of environmental impact grade 3 and 4 area covered 46.9% and it should be restored. The ecological diversity, i.e. species diversity and evenness, showed low class, and especially site 2 which was controlled the user's after 1985 showed the lowest value. Pinus koraiensis community which is a dominant species now in the study area shall be succeed to Quercus aliena community. Robinia pseudoacacia at the eastern wall shall be excluded step by step and it shall be replaced with native species gradually. Ailanthus altissima, Populus ${\times}$ albaglandulosa and Styrax japonica which had higher selection invaded conspicuously the bare land. As the administration planning of the study area, Pinus koraiensis and Styrax japonica shall be thinned out gradually and it shall be replaced with Quercus aliena and Acer palmatum.

• Mycobiology
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• v.45 no.4
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• pp.312-317
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• 2017

Orchidaceous plants have symbiotic relationships with endophytic fungi, including mycorrhizal fungi, which play important roles in the seed germination and growth of the host plants. In this study, endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from three different sites in Korea were analyzed, and it was determined whether fungal communities were preferentially correlated with the sites. The fungal isolates were identified by sequence analysis of the internal transcribed spacer regions of rDNA. In total, 30 species of endophytic fungi, including two species of mycorrhizal fungi belonging to the genus Tulasnella, were identified. Leptodontidium orchidicola showed the highest frequency and was isolated from all root samples. Species diversity and richness were not significantly different among sites. However, the community structure of the endophytic fungi significantly differed among sites, suggesting that the site characteristics affected the community composition of the endophytic fungi colonizing the roots of C. longibracteata. Our findings will aid in developing methods involving the use of symbiotic fungi for orchid conservation and restoration in native habitats.

• Journal of Apiculture
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• v.33 no.4
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• pp.227-237
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• 2018

The majority parasitic bee mites of Thailand in genus Tropilaelaps are infesting colonies of native bees (Apis dorsata) and introduced bees (A. mellifera). The investigation aims to study morphological and genetic variation of Tropilaelaps mites infected different hosts. Adult mites were collected from honey bee brood throughout Thailand. Traditional and geometrical morphometrics were measured on photograph by using TPS program. Additional, COI gene variations were examined by PCR-RFLP and nucleotides sequencing. Tree of mites relationships were constructed by NJ and MP assumptions. Morphometric results indicated T. mercedesae were major species infesting on A. dorsata and A. mellifera. Mophological variation represented at anal and epigynial plate, which the shape of the anal plate apex margin has been key character to identify between T. mercedesae (bell to blunt shape) and T. koenigerum (pear shape). However, the discriminant analysis suggested that geometric results were potential to classify Thai Tropilaelaps populations from different hosts better than traditional morphometric. Otherwise, PCR-RFLP clearly detected the site of Dra I and Xba I digestion of Thai Tropilaelaps morphotypes. The COI sequences of T. koenigerum were founded infesting only A. dorsata in Thailand and four sequences that related to the Thai T. mercedesae morphotypes. The NJ and MP tree were clearly classified Thai Tropilaelaps species which were suggested both from morphological and molecular analysis. This information might be basically of taxonomic status, but this should have implication for controlling these mites in Thailand and other countries.

• Child Health Nursing Research
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• v.27 no.1
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• pp.34-42
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• 2021

Purpose: The purpose of this study was to identify the supportive care needs of parents of preterm children in South Korea using text data from a portal site. Methods: In total, 628 online newspaper articles and 1,966 social network service posts published between January 1 and December 31, 2019 were analyzed. The procedures in this study were conducted in the following order: keyword selection, data collection, morpheme analysis, keyword analysis, and topic modeling. Results: The term "yirundung-yi", which is a native Korean word referring to premature infants, was confirmed to be a useful term for parents. The following four topics were identified as the supportive care needs of parents of preterm children: 1) a vague fear of caring for a baby upon imminent neonatal intensive care unit discharge, 2) real-world difficulties encountered while caring for preterm children, 3) concerns about growth and development problems, and 4) anxiety about possible complications. Conclusion: Supportive care interventions for parents of preterm children should include general parenting methods for babies. A team composed of multidisciplinary experts must support the individual growth and development of preterm children and manage the complications of prematurity using highly accessible media.

• Bulletin of the Korean Chemical Society
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• v.8 no.4
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• pp.280-285
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• 1987

FMN-dependent glycolate oxidase from spinach is inactivated by diethyl pyrocarbonate at pH 7.0. Inactivation of both apo- and holoenzyme by diethyl pyrocarbonate follows pseudo-first-order kinetics and first order with respect to the reagent. A series of difference spectra of inactivated and native enzymes show a single peak at 240 nm, indicating the modification of histidyl residues. No decrease in absorbance at around 280 nm due to formation of O-carbethoxytyrosine is observed. The rate of inactivation is dependent on pH, and the data for pH dependent rates implicate the involvement of a group with a pKa of 6.9. The activity lost by treatment with diethyl pyrocarbonate could be almost fully restored by incubation with 0.75M hydroxylamine. The reactivation by hydroxylamine and the pH dependence of inactivation are also consistent with that the inactivation is due to modification of histidyl residues. Although coenzyme FMN is without protective effect, the substrate glycolate, the product glyoxylate, and two competitive inhibitors, oxalate and oxalacetate, provide marked protection against the inactivation of the holoenzyme. These results suggest that the inactivation of the oxidase by diethyl pyrocarbonate occurs by modification of essential histidyl residue(s) at the active site.

• Ocean and Polar Research
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• v.44 no.2
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• pp.161-177
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• 2022

Microalgae can attach to the surface of ships and then spread to various areas by means of ship transport. The introduction of invasive species through ships is recognized as a marine problem worldwide. Identification of attached microalgae is necessary to investigate such movement between countries through ships. In the present study, through analytical methods we reviewed research data to identify the taxa of domestic attached microalgae and assess the ecological impacts of such microalgae. A total of 87 genera and 153 species (143 species of diatoms, 10 species of cyanobacteria, and 4 genera of dinoflagellates) were identified as native attached microalgae in Korea, and diatoms accounted for 93% of the total. Most of these attached microalgae were identified through research on natural substrates such as seaweeds and bedrock, and some were also identified through experiments using artificial adherent plates. To date, there is no information on microalgae attached to international ships and introduced into Korea. Molecular genetic analysis and systematic management through on-site sampling of international ships, microscopic analysis, and meta-barcoding are necessary to assess the inflow and spread path of hull-attached marine alien species and evaluate the risk they pose to the domestic ecosystem.