The major histocompatibility complex (MHC) plays well-defined roles in eliciting immune responses and combating infectious diseases. The major histocompatibility complex of cattle is referred to as BoLA (Bovine Lymphocyte Antigen). This genetic system is among the most polymorphic. In the present study, polymorphism of the BoLA- DRB3.2 locus in three Bos indicus breeds viz., Sahiwal, Rathi and Hariana was studied by polymerase chain reaction restriction fragment length polymorphism technique using the enzymes RsaI, Bst Y1 and Hae III. Both Sahiwal and Rathi are good Indian dairy breeds and survive under tough tropical conditions, while Hariana is a prominent dual-purpose breed reared both as a dairy animal and for bullock production. A total of 30 different BoLADRB3.2 alleles were observed to be present in the 3 Bos indicus breeds. Certain alleles were common amongst the three breeds while there were others that were unique to each breed. Allelic distribution amongst the three breeds showed that each breed had a unique allelic distribution pattern that was different from each other and also different from the earlier breeds studied so far for the existence of allelic variation at this locus. A dendogram was constructed based on the frequencies of the BoLA-DRB3 alleles using the UPGMA method. The Rathi and Hariana animals were genetically the most apart. The Hariana animals clustered on a different branch from the other two breeds viz. the Rathi and the Sahiwal. The smallest genetic distances for the DRB3 alleles were those between Sahiwal and Rathi (0.5461) while genetic distance between Hariana and Sahiwal was 0.6123. A comparison of the allelic frequencies of the BoLADRB3.2 locus in these 3 breeds viz. Sahiwal, Hariana and Rathi with the allelic frequencies present in the previously characterized Bos indicus Kankrej breed, which is a dual purpose breed reared both as a draught and a dairy animal, showed that the Bos indicus Sahiwal and Rathi breeds clustered into one group while the Hariana and Kankrej breeds formed another group. The Rathi and Sahiwal showed the least genetic distance of 0.5461 amongst the breeds whereas the Rathi and Kankrej, with a Nei''s genetic distance of 1.1622, were genetically the most distant apart.
Quercus gilva Blume is an evergreen-leaved tree that belongs to Facaceae and grows in tropical and warm-temperate regions, such as Japan, Taiwan, China and Korea. This study was carried out to analyze the distribution and growth characteristics of Q. gilva in the native range. In Korea, this species was distributed in Jeju Island between 80 and 350 meter above sea level, and mainly in the south-western regions of the island from 100 to 200 meter above sea level. The average height of Q. gilva was $9.8{\pm}1.9$ meter, with most trees 9 and 12 meter tall. The average diameter at breast height was $22.6{\pm}6.8$ centimeter, with the majority between 20 and 30 centimeter. Several coppice shoots ($3.8{\pm}2.1$ pieces) were formed from stumps and inferred by frequent cuttings for use. Almost Q. gilva individuals(96.2%) were covered with kinds of vines(18 species), and evergreen Trachelospermim asiaticum (Siebold et Zucc.) Nakai was major species. The analysis of damage of vines revealed that the most dead branches were found with 9 to 12 meter height of Q. gilva, and the more vines attached, the larger damage to Q. gilva individuals. A continuous monitoring and investigation would be required to preserve this species in their habitats.
Purpose - With the rapid economic development, great changes have happened in the Chinese beer market. The scale of imported beer is increasing. However, the sales of native Chinese beer decrease in recent years. It is because more Chinese customers prefer beer with unique attributes than those with lower prices. In order to take an advantage in this market, practitioners should develop new products to satisfy Chinese customers. Scholars have also focused on beer characteristics to estimate consumers' behavior for improving the development of beer industry. However, most studies are theoretically centered on marketing strategy management or general understanding of the market. It is not enough for us to explain customers' consumption patterns. Some empirical research did attempt to find out beer attributes that may influence their choice behavior. However, they failed to verify what can increase customers' utility through a new product. More importantly, few evidences can be found in the literature. Therefore this study explores the major characteristics of imported beer which may associate with customers' preferences. Research design, data, and methodology - With the results of literature review and focus group interview, we found out four main imported beer attributes and the levels of each factor. A pre-test is constructed to check the problems in the questionnaire protocol. With the results of pre-test, the revised questionnaire is processed among customers who have purchased and had imported beer in China regions such as Beijing, Shanghai, Jiangsu, Guangdong and so on. The choice-based conjoint analysis is applied to analyze the useful data of 205. Results - Results indicate that choice-based conjoint analysis is the effective method to estimate customers' choice behavior. Results also indicate that country of origin, price, taste, and package affect customers' preference and the importances of imported beer attributes are significantly different. Conclusions - Country of origin, price, taste, and package are important for customers to make an imported beer choice decision. Marketers should consider these determinants and their importance to develop new products. Moreover, choice-based conjoint analysis should be the useful method for practitioners to develop a new product of imported beer for sustainable business in the competitive industry.
In this paper, we discuss why two different surface forms appear in loanwords for English and German /${\int}$/ In Korean, a vowel is inserted into loanwords if a consonant cannot be properly syllabified. Therefore, /${\int}$/ in some positions of loanwords trigger vowel insertion. Interestingly, /${\int}$/s in the onset cluster of English and German words were borrowed in Korean as Iful with the inserted vowel [u] whereas If Is in the coda position of English and German words were borrowed as Ifil with the inserted vowel [i]. For example, 'shrimp' is adopted as [${\int}urimphi$] whereas 'rush' is adopted as [$ra{\int}i$]. In this paper, we attempt to find out the phonetic reason for the distribution of the surface forms of /${\int}$/. We assume that since the formant frequency of [i] is higher than that of [u], the peak frequency of /${\int}$/ with the surface form of [${\int}$i] in loanwords may be higher than that of /${\int}$/ with the surface form of [${\int}u$]. We also assume that duration may be another factor for the distribution of [${\int}i$] and [${\int}u$]. Since /${\int}$/ and /u/ use lip rounding whereas /i/ doesn't, the duration for [${\int}i$] might be longer than that of [${\int}u$]. German supports our assumption. /${\int}$/ in the onset cluster is longer than /${\int}$/ in the coda position. It also has higher peak frequency than that of /${\int}$/ in the coda position. In loanwords, ${\int}$ in the onset cluster is borrowed as [${\int}u$] as in Spiegel whereas /${\int}$/ in the coda position is borrowed as [${\int}i$] as in Bosch. English, however, does not support our assumption. Peak frequency of [${\int}$] depends on the preceding vowel, not on its position in the syllable structure. If the preceding vowel is front, then the peak freuency of the following of the following /${\int}$/ is high but if the preceding vowel is back, than the peak frequency of the following /${\int}$/ is low. The peak frequency of /${\int}$/ in the onset cluster seems to be in between. As we assumed, however, the duration of /${\int}$/ in the coda position is longer than of /${\int}$/ in the onset cluster. With the mixed results, we question whether Koreans really hear two different xounds for /${\int}$/ in English words. For the future experiment, we would like to perform the perception tet for /${\int}$/ in English words.
This study was attempted to comparative investigate the types and regional distribution of the endocrine cells in several vertebrates immunohistochemically using seven antisera. From carp pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were mainly distributed at the periphery and a few cells occupied the central region of the islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, and som - and BPP-immunoreactive cells were located at the central region. From frog pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were distributed at the periphery of the islets, and glucagon- and BPP-immunoreactive cells were found as single cell or as small groups located between the pancreatic acini. From snake pancreas could be observed 3 types which are insulin-, glucagon- and som -immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, and they also were scattered at the periphery of the large islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, whereas som-immunoreactive cells were occupied the central region. From Ogolgae pancreas could be observed 4 types which are insulin-, glucagon-, som-and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, but at the periphery of the large one. Glucagon- immunoreactive cells were distributed at the periphery of the small islets and in the large islets showed scattering entired. Som-immunoreactive cells were distributed at the periphery of the small islets and in the large islets were located at the central region. A small numbers of BPP-immunoreactive cells were located at the periphery of the small islets and the exocrine regions. From the pancreas of the Korean native goat could be observed 6 types which are insulin-, glucagon-, som-, BPP-, 5-HT- and porcine-CG-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were located at the periphery of the islets, but a tew were scattered at the central region of islets and in the epithelium of the secretory duct. Glucagon-, BPP-, 5-HT- and porcine CG-immunoreactive cells were distributed at the periphery of the islets. These findings indicated that the regional distribution patterns and cell types of pancreatic endocrine cells in vertebrates varies considerably among phylogenetically different vertebrates.
The life form, the origin and the time of introduction of those naturalized plants grown in Jeju-Do, korea were grasped by conducting the documentary survey, and the field survey thereof. The naturalized plants were total 199 taxa including 185 species, 12 varieties and 2 cultivar, which belong to 115 genera, 32 families. The classification of families, there were 43 taxa of Compositae ($21.6\%$), 32 taxa of Gramineae ($16.8\%$), 17 taxa of Legumlinosae ($8.5\%$), and 13 taxa of Cruciferae ($6.5\%$). As the life forms of the naturalized plants in Jeju Island were there 91 taxa of annual plants, 31 taxa of biennial plants, 16 taxa of annual or biennial plants, 57 taxa of perennial plants, and 4 taxa of trees. The distribution of the naturalized plants, the were 29 taxa thereof were located in Jeju Island, 20 taxa in Jeju Island as well as in the southern part of Korean Peninsula, 33 taxa in Jeju Island as well as in the central part of Korean Peninsula, and 117 taxa in the entire area of South Korea. As 89 taxa thereof were originated from America, 69 taxa from Europe, 2 taxa from Africa, 22 taxa from Asia, 1 taxa from Oceania, and 16 taxa from other provinces. As 38 taxa thereof had been introduced into Jeju Island before 1921, 23 taxa from 1922 to 1963, and 138 taxa since 1964.
Ju Hyoun Wang;Jung Soo Han;Jun Kil Choi;Hwang Goo Lee
Korean Journal of Environmental Biology
/
v.41
no.2
/
pp.101-108
/
2023
Recently, in relation to climate change, many studies have been conducted to predict the potential habitat area and distribution range of tilapia and the suitability of habitat for each species. Most tilapia are tropical fish that cannot survive at water temperatures below 10 to 12℃, although some tilapia can survive at 6 to 8℃. This study predicted habitable areas and the possibility of spreading of habitat ranges of tilapia (Oreochromis niloticus and Oreochromis aureus) known to inhabit domestic streams. Due to climate change, it was found that habitats in the Geum River, Mangyeong River, Dongjin River, Seomjin River, Taehwa River, Hyeongsan River, and the flowing in East Sea were possible by 2050. In addition, it was confirmed that tilapia could inhabit the preferred lentic ecosystem such as Tamjin Lake, Naju Lake, Juam Lake, Sangsa Lake, Jinyang Lake, Junam Reservoir, and Hoedong Reservoir. In particular, in the case of tilapia, which lives in tributaries of the Geumho River, Dalseo Stream, and the Nakdong River, its range of habitat is expected to expand to the middle and lower of the Nakdong River system. Therefore, it is judged that it is necessary to prepare physical and institutional management measures to prevent the spread of the local population where tilapia currently inhabits and to prevent introduction to new habitats.
Journal of the Korean Institute of Traditional Landscape Architecture
/
v.35
no.2
/
pp.77-86
/
2017
A study on the population ecology of gregarious Wisteria habitat was conducted in Beomeosa temple, Busan. The area has been protected since it was designated as No. 176 natural monument in 1966. Such a large habitat of native wisteria (Wisteria floribunda DC.) in Korea is very rare so that it has a very high academic value. However, there are no high-resolution researches on the distribution and ecology of wisteria in the Gregarious Wisteria Habitat of Beomeosa Temple. The study aimed to identify the distribution and characteristics of wisteria populations in the Wisteria habitat. The study identified the distribution of Wisteria, RCC, the climbing direction, the plant and flora in the research area in the Gregarious Wisteria Habitat of Beomeosa Temple in Busan and the surrounding areas based on an on-site research. As a result, the total number of the wisteria populations in the research area was confirmed to be 1,158 and the RCC of wisteria was on average 69.0 mm (${\pm}45.5$) and in maximum 365 mm. In terms of the climbing direction, the number of wisteria that climbs clockwise or counterclockwise was determined to be 40. It was identified that there are 28 taxa of the climbing plant species and the wisteria of the same kind was the most favorable. The populations were found to be dispersed adjacent to the valleys, and were found to be maintained by constant disturbance. 76.6 % of the population was found to be distributed in the valley forest, and the Carpinus tschonoskii-Pseudosasa japonica forest, Pueraria lobata community and Pinus densiflora forest were found to be relatively dispersed. The study proposed to conduct the follow-up researches to preserve the wisteria that is spreading in this research area, the wisteria with the highest RCC and regional habitat through continuous monitoring; and maintain the protection area of No. 176 natural monument; and discussed the management measures and approaches that reflect the habitat.
Journal of The Korean Society of Grassland and Forage Science
/
v.6
no.2
/
pp.124-130
/
1986
To find out the optimum split application ratios of fertilizer nitrogen and potassium in the newly opened pasture at spring, a field experiment was conducted. Two levels of nitrogen and potassium application and three split application ratios of both fertilizer were treated by split design with 4 replications. The experiment was carried out from March, 1984 to October, 1985 on the experimental field of the Livestock Experiment Station in Suweon. The results obtained are summarized as follow: 1. Total DM yield was not showed signigicant difference among split application ratios of nitrogen and potassium. 2. DM yield of grasses was 4,854 kg/ha in heavy basal application of N and $K_2$O (160 kgN: 140 kg $K_2$O/ha), it was significantly increased by 16% than of conventional dressing (80 kg N: 70 kg $K_2$O/ha). But there was almost no difference in dry matter among N and $K_2$O split application ratios. 3. Dry matter yield of legume in heavy basal dressing was decreased by 23% than that of conventional dressing. In the methods of NK application, dry matter yield of legume was tended to decrease by 3% with equal application and 9% with heavy dressing in autumn, when compared with that of heavy dressing in spring. 4. There was no significant difference in dry matter yield of native species between different basic fertilization levels, but dry matter yield of native species was decreased by 6% with equal application and more increased by 11% with heavy dressing in autumn, when compared with the yield of heavy dressing in spring. 5. In aspect of the botanical composition, the percentage distribution of grasses was increased and the ratio of legume was greatly decreased by heavy dressing at basal fertilization. Also for good maintenance of botanical composition, it is suggested that the equal split application of N and $K_2$O was desirable.
Ha, Woel-Kyu;Juhn, Suk-Lak;Kim, Jung-Wan;Lee, Soo-Won;Lee, Jae-Young;Shon, Dong-Hwa
Korean Journal of Food Science and Technology
/
v.26
no.1
/
pp.74-80
/
1994
As a preliminary study about the reduction of the antigenicity of whey protein isolate(WPI) by treatment of chymotrypsin, trypsin, pancreatin, and protease from Aspergillus oryzae, the properties and antigenicities of whey protein hydrolysates(WPH) were investigated. When degrees of hydrolysis (DH) were measured by use of trinitrobenzensulfonic acid(TNBS), the DH of the WPH treated by pancreatin or protease from Aspergillus oryzae$(5.05{\sim}11.47)$ were much higher than those of the tryptic or chymotryptic WPH$(15.67{\sim}20.20)$. And the pretreatments of heat$(75^{\circ}C)$, 20 min and/or pepsin resulted in higher DH of WPH, generally. When the molecular distributions of the WPH were determined by high performance size exclusion chromatography(HPSEC), the ratios of polypeptides with molecular weight more than 10kDa ranged from 12% to 36%, and the average molecular weights and the average peptide lengths of the WPH were $4,252{\sim}9,132$ dalton and $38{\sim}83$ amino acids, respectively. And there was no bitter taste in all of the WPH. Results of SDS-PAGE showed that most of intact native proteins were eliminated by the enzymatic hydrolysis but there were a few bands of peptides larger than 14.2 kDa in some WPH. When antigenicity was assayed by competitive inhibition enzyme-linked immunosorbent assay(cELISA), monovalent antigenicity of WPH to rabbit anti-WPI antiserum were lowered to $10^{-1.7}-10^{-4.9}$ times and less by the enzymatic hydrolysis. And the pretreatments of heat and pepsin resulted in the lowest antigenicicy within a group of enzymatic hydrolysis, especially in case of the pancreatic hydrolysate(PDP) whose antigenicity was found almost to be removed.
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