Browse > Article
http://dx.doi.org/10.5713/ajas.2009.90043

Genetic Variation of the Major Histocompatibility Complex DRB3.2 Locus in the Native Bos indicus Cattle Breeds  

Behl, Jyotsna Dhingra (National Bureau of Animal Genetic Resources)
Verma, Naresh Kumar (National Bureau of Animal Genetic Resources)
Behl, Rahul (National Bureau of Animal Genetic Resources)
Sodhi, Monika (National Bureau of Animal Genetic Resources)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.11, 2009 , pp. 1487-1494 More about this Journal
Abstract
The major histocompatibility complex (MHC) plays well-defined roles in eliciting immune responses and combating infectious diseases. The major histocompatibility complex of cattle is referred to as BoLA (Bovine Lymphocyte Antigen). This genetic system is among the most polymorphic. In the present study, polymorphism of the BoLA- DRB3.2 locus in three Bos indicus breeds viz., Sahiwal, Rathi and Hariana was studied by polymerase chain reaction restriction fragment length polymorphism technique using the enzymes RsaI, Bst Y1 and Hae III. Both Sahiwal and Rathi are good Indian dairy breeds and survive under tough tropical conditions, while Hariana is a prominent dual-purpose breed reared both as a dairy animal and for bullock production. A total of 30 different BoLADRB3.2 alleles were observed to be present in the 3 Bos indicus breeds. Certain alleles were common amongst the three breeds while there were others that were unique to each breed. Allelic distribution amongst the three breeds showed that each breed had a unique allelic distribution pattern that was different from each other and also different from the earlier breeds studied so far for the existence of allelic variation at this locus. A dendogram was constructed based on the frequencies of the BoLA-DRB3 alleles using the UPGMA method. The Rathi and Hariana animals were genetically the most apart. The Hariana animals clustered on a different branch from the other two breeds viz. the Rathi and the Sahiwal. The smallest genetic distances for the DRB3 alleles were those between Sahiwal and Rathi (0.5461) while genetic distance between Hariana and Sahiwal was 0.6123. A comparison of the allelic frequencies of the BoLADRB3.2 locus in these 3 breeds viz. Sahiwal, Hariana and Rathi with the allelic frequencies present in the previously characterized Bos indicus Kankrej breed, which is a dual purpose breed reared both as a draught and a dairy animal, showed that the Bos indicus Sahiwal and Rathi breeds clustered into one group while the Hariana and Kankrej breeds formed another group. The Rathi and Sahiwal showed the least genetic distance of 0.5461 amongst the breeds whereas the Rathi and Kankrej, with a Nei''s genetic distance of 1.1622, were genetically the most distant apart.
Keywords
Bovine Lymphocyte Antigen; Polymorphism; Bos indicus; DRB3;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Behl, J. D., N. K. Verma, R. Behl, M. Mukesh and S. P. S. Ahlawat. 2007. Characterization of genetic polymorphism of the bovine lymphocyte antigen DRB3.2 locus in Kankrej cattle (Bos indicus). J. Dairy Sci. 90:2997-3001   DOI   ScienceOn
2 De, S. and R. K. Singh. 2006. Identification of new MHC-DRB3 alleles from Indian (Bos indicus) cattle. Anim. Genet. 37:595-607   DOI   ScienceOn
3 Dechamma, H. J., C. Natarajan, V. V. Suryanaryanan and T. J. Rasool. 1998. Nucleotide Sequence of DRB exon of major histocompatibility complex from an Indian zebu cattle breed. Ind. J. Exp. Biol. 36(9):879-886   PUBMED
4 Dietz, A. B., J. C. Detilleux, A. E. Freeman, D. H. Kelly, J. R. Stable and M. E. Kehrli Jr. 1997a. Genetic association of bovine lymphocyte antigen DRB3 alleles with immunological traits of Holstein cattle. J. Dairy Sci. 80:400-405   DOI   ScienceOn
5 Glass, E. J., P. M. Preston, A. Springbett, S. Craigmile, E. Kirvar, G. Wilkie and C. G. Duncan Brown. 2005. Bos Taurus and Bos indicus (Sahiwal) calves respond differently to infection with Theileria annulata and produce markedly different levels of acute phase proteins. Int. J. Parasitol. 35:337-347   DOI   ScienceOn
6 Levene, H. 1949. On a mating problem in genetics. Ann. Math Stat 20:91-94   DOI   ScienceOn
7 Mossafer, J. and M. R. Nassiry. 2005. Identification of bovine lymphocyte antigen DRB3.2 alleles in Iranian Golpayegani cattle by DNA test. Asian-Aust. J. Anim. Sci. 18(12):1691-1695
8 Pipalia, D. L., C. G. Joshi, D. N. Rank, B. P. Brahmkshtri and V. Solanki. 2004. PCR-SSCP typing of MHC in cattle and buffaloes. Ind. J. Anim. Sci. 74(6):637-639
9 Takeshima, S., Y. Nakai, M. Ohta and Y. Aida. 2002. Characterization of DRB3 alleles in the MHC of Japaneese Shorthorn Cattle by Polymerase chain reaction-sequence based typing. J. Dairy Sci. 85:1630-1632   DOI   ScienceOn
10 Miretti, M. M., J. A. Ferro, M. A. Lara and E. P. Contel. 2001. Restriction fragment length polymorphism (RFLP) in exon 2 of the BoLA -DRB3 gene in South American Cattle. Biochem. Genet. 39(9-10):311-324   PUBMED
11 Russel, G. C., K. L. Marello, A. Gallagher, D. J. Mckeever and R. L. Spooner. 1994. Amplification and sequencing of expressed DRB second exons from Bos indicus. Immunogenetics 39:432-436   PUBMED
12 Udina, I. G., E. E. Karamysheva, G. E. Sulimova, S. P. Pavelenko, S. O. Turkova, A. R. Orlova and L. K. Ernst. 1998. Comparitive analysis of Ayrshire and Black Pied cattle breeds by histocompatibility markers. Russ. J. Genet. 34(12):1421-1427
13 Mizuki, M., S. Ohno, H. Ando, T. Sato, T. Imanshi, T. Gojobori, M. Ishihara, M. Ota, Z. Geng, L. Geng, G. Li, M. Kimura and H. Inoko. 1997. Major Histocompatibility complex class II alleles in Kazak and Han populations in the silk Route of Northwestern China. Tissue Antigens 50:527-534   DOI   ScienceOn
14 Acharya, C. P., D. L. Pipalia, D. N. Rank, C. G. Joshi, J. V. Solanki and R. R. Shah. 2002. Detection of BoLA-DRB3 gene polymorphism in Gir and Kankrej cattle using PCR-RFLP. Ind. J. Anim. Sci. 72(8):680-683
15 Hansen, E. W. 1994. Ganaderia bovina de raza criolla en el noroeste argentino . Universidad Nacional de Jujuy, San Salvador de Jujuy, Argentina
16 Ripoli, M. V., L. P. Lirron, J. C. Luca De, F. Rojas, F. N. Dulout and G. Giovambattista. 2004. Gene frequency distribution of the BoLA-DRB3 locus in Saavedreno Creole dairy cattle. Biochem. Genet. 42(7/8):231   DOI   ScienceOn
17 Sigurdardottir, S., C. Borsch, K. Gustafsson and L. Andersson. 1991. Cloning and sequence analysis of 14 DRB alleles of the bovine major histocompatibility complex by using the polymerase chain reaction. Anim. Genet. 22:199-209   DOI   PUBMED   ScienceOn
18 Burke, M. G., R. T. Stone and N. E. Muggli-Cocket. 1991. Nucleotide sequence and northern analysis of a bovine major histocompatibility class II DR$\beta$-like cDNA. Anim. Genet. 22:343-352   DOI   PUBMED   ScienceOn
19 Lewin, H. A., G. C. Russel and E. J. Glass. 1999. Comparitive organization and function of the major histocompatibility complex of domesticated cattle. Immunol. Rev. 167:145-58   DOI   ScienceOn
20 Page, R. D. M. 1996. TREEVIEW: An application to display phylogenetic trees on personal computers. Available from http://taxonomy.zoology.gla.ac.uk/rod/rod.html. Computer Applications in Biosciences 12:357-358
21 Giovambattista, G., C. D. Golijow, F. N. Dulout and M. M. Lojo. 1996. Gene frequencies of DRB3.2 locus of Argentina Creole cattle. Anim. Genet. 27:55-56   DOI   PUBMED   ScienceOn
22 Schwaiger, F. and J. Epplen. 1995. Exonic MHC- DRB polymorphisms and intronic simple repeat sequences. Janus' faces of DNA sequence evolution. Immunol. Rev. 143:199-224   DOI   ScienceOn
23 Nivsarkar, A. E., P. K. Vij and M. S. Tantia. 2000. Animal genetic resources of India - cattle and buffaloes. Directorate of Information and Publication of Agriculture. Indian Council of Agricultural Research Krishi Anusandhan Bhawan. New Delhi 110012. pp. 79-82
24 Edwards, S. V. and P. W. Hedrick. 1998. Evolution and ecology of MHC molecules: from genomics to sexual selection. Trends Ecol. Evol. 13:305-311   DOI   ScienceOn
25 Van Eijk, M. J., T. Stewart, J. A. Haynes and H. A. Lewin. 1992. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Anim. Genet. 23:483-96   DOI   PUBMED   ScienceOn
26 Ballingall, K. T., A. Luyia, G. L. Rowlands, J. Sales, A. J. Musoke, S. P. Morzaria and D. J. Mckeever. 2004. Bovine leukocyte antigen major histocompatibility complex class II $DRB\ding{83}$2703 and DRB*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with sporozoite p67 antigen. Infect. Immun. 72(5):2738-2741   DOI   ScienceOn
27 Mason, I. L. 1996. A world dictionary of livestock breeds, types and varieties.$4^{th}$ ed. CAB Int., Wallingford, UK
28 Aravindakshan, T. V. and A. M. Nainar. 1999. Genetic polymorphism of the BoLA-DRB3 gene in Jeresy crossbred and Ongole cattle revealed by PCR-RFLP. Ind. J. Anim. Sci. 69(6):424-27
29 Guglielmone, A. A., A. J. Mangold, D. H. Aguirre, A. C. Bermudez and A. B. Gaido. 1991. Comparacion de la raza criolla con otros biotipos bovinos respecto al parasitismo por Boophilus microplus e infecciones naturales de Babesia bovis, Babesia bigemina y Anaplasma marginale pp 1-6. En. Ganado Bovino Criollo. Orientacion Grafica Editora, Buenos Aires, Argentina, Tomo2
30 Nei, M. 1972. Genetic distance between populations. Am. Nat. 106:283-292   DOI   ScienceOn
31 Langella, O. 2002. POPULATIONS, version 1.2.28. Available from http://www.pge.cnrs-gif.fr/bioinfo/populations/
32 Gelhaus, A. L., L. Schnittger, D. Mehlitz, R. D. Horstmann and C. G. Meyer. 1995. Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles. Anim. Genet. 26:147-153   DOI   PUBMED   ScienceOn
33 Gilliespie, B. E., B. M. Jayarao, H. H. Dowlen and S. P. Oliver. 1999. Analysis and frequency of bovine lymphocyte antigen DRB 3.2 alleles in Jersey cows. J. Dairy Sci. 82:2049-2053   DOI   ScienceOn
34 Sambrook, J. and D. W. Russel. 2001. Molecular cloning. A Laboratory Manual. Silver staining of DNA in non-denaturing polyacrylamide gels. 3rd Ed. Cold Spring Harbor, New York, Vol. 3, pp. A9.6-A9.7
35 Sulimova, G. E., I. G. Udina, G. O. Shaikhaev and I. A. Zakharov. 1995. DNA polymorphism at the BoLA-DRB3 gene of cattle in relation to resistance and susceptibility to leukemia. Russ. J. Genet. 31(9):1105-1109
36 Maillard, J.C., C. Renard, P. Chardon, I. Chantal and A. Bensaid. 1999. Characterisation of 18 new BoLA-DRB3 alleles. Anim. Genet. 30:200-203   DOI   ScienceOn
37 Nassiry, M. R., F. E. Shahroodi, J. Mossafer, A. Mohammadi, E. Manshad, S. Ghazanfari, M. R. Mohammad Abadi and G. E. Sulimova. 2005. Analysis and frequency of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle. Russ. J. Genet. 41(6):817-822
38 Rabasa, C. E. 1993. Comportamiento comparativo del ganado criollo, pp. 14-7. En Ganado Bovino criollo. Orientacion Grafica Editore, Buenos Aires, Argentina, Tomo3
39 Schwaiger, F., E. Weyers, J. Buitkamp, A. Crawford and J. Epplen. 1994. Interdependent MHC-DRB exon plus-intron evolution in artodactyls. Mol. Biol. Evol. 11:239-249   DOI   PUBMED
40 Dietz, A. B., N. D. Cohen, L. Timms and M. E. Kehrli Jr. 1997b. Bovine lymphocyte antigen class II alleles as risk factors for high somatic cell counts in milk of lactating dairy cows. J. Dairy Sci. 40:406-412
41 Sharif, S., B. A. Maillard, B. N. Wilkie, J. M. Sargeant, H. M. Scott, J. C. M. Dekkers and K. E. Leslie. 1998. Associations of the bovine major histocompatibility DRB3 (BoLA DRB3 alleles) with the occurrence of disease and milk somatic cell score in Canadian Dairy cattle. Anim. Genet. 29:185-193   DOI   ScienceOn
42 Andersson, L. and S. Mikko. 1995. Generation of MHC Class II diversity by intra and intergenic recombination. Immunol. Rev. 143:5-12   DOI   PUBMED   ScienceOn