• Journal of Microbiology and Biotechnology
• /
• v.27 no.8
• /
• pp.1461-1471
• /
• 2017

Escherichia coli heat-labile enterotoxin (LT) and its non-toxic mutant (LTm) are well-known powerful mucosal adjuvants and immunogens. However, the yields of these adjuvants from genetically engineered strains remain at extremely low levels, thereby hindering their extensive application in fundamental and clinical research. Therefore, efficient production of these adjuvant proteins from genetically engineered microbes is a huge challenge in the field of molecular biology. In order to explore the expression bottlenecks of LTm in E. coli, we constructed a series of recombinant plasmids based on various considerations and gene expression strategies. After comparing the protein expression among strains containing different recombinant plasmids, the signal sequence was found to be critical for the expression of LTm and its subunits. When the signal sequence was present, the strong hydrophobicity and instability of this amino acid sequence greatly restricted the generation of subunits. However, when the signal sequence was removed, abundantly expressed subunits formed inactive inclusion bodies that could not be assembled into the hexameric native form, although the inclusion body subunits could be refolded and the biological activity recovered in vitro. Therefore, the dilemma choice of signal sequence formed bottlenecks in the expression of LTm. These results reveal the expression bottlenecks of LTm, provide guidance for the preparation of LTm and its subunits, and certainly help to promote efficient preparation of this mucosal adjuvant protein.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.38 no.1
• /
• pp.91-101
• /
• 2012

This study's purpose is to research the antioxidant power of Calaguala (Polypodium leucotomos, PL) as an oral supplement: Calaguala, a native of Central America, is a fern which belongs to the family polypodiaceae protecting the skin from the ultraviolet and active oxygen free radicals. The total 14 volunteers, each female 7 & male 7, were divided into 2 groups, then exposed to UV for 30 min after taking Calaguala and placebo. The total antioxidant capacity (TAC) was analyzed by taking the blood samples from anticubital vein 3 hours after UV exposure. First, Calaguala siginifcantly decreased the concentration of active oxygen free radicals 4 weeks later. The concentration of active oxygen free radical in PL group declined more than placebo group, when measured 30 min and 3 hours after UV exposure. Second, Calaguala did not significantly increase TAC 4 weeks later, but significantly decreased TAC 3 hours after exposure, when compared with placebo group. Now days, the photo allergy and photo aging caused by air pollution and ozone destruction have drastically increased, and thus the amount of UV radiation reaching the earth's surface increased. The increase of active free oxygen radicals resulted in accelerating the diseases and aging. The first superficial protecting protection by reflection & dispersion and the second body internal protection by taking oral photo antioxidant such as Calaguala should be continuously researched.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.12
• /
• pp.1659-1666
• /
• 2000

Two experiments were conducted to determine the effects of Leucaena leucocephala (leucaena) and Gliricidia sepium (gliricidia) supplementation on intake, digestion, outflow rates, microbial protein yield and live-weight changes in sheep fed with ammoniated rice straw (ARS). In experiment 1, three rumen cannulated Japanese Corriedale wether (mean body weight of 35.6 kg) in $3{\times}3$ Latin Square Design were used. Animals were fed ad libitum ARS alone, or supplemented with 200 g of either leucaena or gliricidia. In experiment 2, twenty-four growing native Philippine sheep with average body weight of $13.5{\pm}0.25kg$ were used in a completely randomized design (CRD) and offered similar diets to those of experiment 1. Supplementation increased total dry matter intake and nutrient digestibility except for fiber (p<0.05) without affecting ARS consumption. Nitrogen balance revealed that absorbed and retained N was significantly higher in leucaena and gliricidia. The significant improvement in N utilization and more digestible OM intake brought about by the inclusion of leucaena and gliricidia to ARS resulted in increased (p<0.05) microbial N yield. Efficiency of microbial N supply in supplemented group was not significantly different, but higher (p<0.05) than the 24.92 g N/kg DOMR for ARS group. Liquid outflow rate was 7.8 and 6.8 %/h, while the solid phase of rumen digesta was 4.4 and 3.8 %/h for the leucaena and gliricidia group respectively, which were significantly higher than 5.30 and 2.50 %/h in the control diet. The increase in total DMI resulted to higher (p<0.01) growth performance and efficient feed utilization. Average daily gain (ADG) was 19.3, 34.6 and 33.9 g/d for the ARS, leucaena and gliricidia respectively. It is therefore concluded that addition of leucaena and gliricidia to ARS in could increase nutrient intake and digestibility, subsequently improving N utilization and livestock performance.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.49 no.2
• /
• pp.23-31
• /
• 2012

With advance of high bandwidth network infrastructure, the requirement is dramatically increasing to cooperate between the users who are far from each other and make use of bulk data. However, as the prominent data transmission protocol, it is well known that TCP suffers some degrees of inefficiency for bulk data transmission when RTT is relatively big. So, some works are on going to suggest a new transmission method to utilize the bandwidth in effective. UDT(UDP-based Data Transfer protocol) is one of these. It is a UDP based application level protocol which can guarantee reliability and stability. much like as TCP. In this paper, we present a design and implementation of UDT based bulk data transmission tool by applying parallel and compressive techniques. The implementation result is examined to measured its performance improvement on a real test-bed, and then compared with existing bulk data transmission tools. Experimental results show that proposed tool is more stable and shows greater performance than that of native UDT. Especially, the performances show 244% improvement in RTT 400ms without losses and 229% in RTT 250ms with 0.005% losses respectively.

• Journal of The Korean Association For Science Education
• /
• v.36 no.3
• /
• pp.499-507
• /
• 2016

A flipped learning class was held in an attempt to overcome the limits of lecture-type classes in pre-service science teacher training and to provide a student-oriented education suitable for digital native generation. The principles of teaching-learning in flipped learning were applied to the general ADDIE model to design the class; learning materials were developed accordingly. The developed flipped learning materials and class design were verified for their validity using an expert panel's Delphi method and validity test, in which the validity was verified with 0.75 CVR. The developed flipped learning materials were applied to the theory of science education and the instructional effectiveness was analyzed. The results suggest that the students' motivation to study, interest, and confidence in learning increased; however, their satisfaction in class decreased by 30% as compared to the lecture-type class and their self-confidence in the improvement of their academic achievement was not sufficient. In order for a flipped learning class to be successful, the class should be small in size, which would ensure appropriate teacher-student communication and individualized learning; also, the students' burden of learning should be reduced and accessibility to video materials for pre-class learning should be reinforced.

• Journal of the Korean Institute of Traditional Landscape Architecture
• /
• v.31 no.4
• /
• pp.62-69
• /
• 2013

The origin of natural planting, which is getting the spotlight in modern time facing natural and environmental problems, can be found from wild gardens. They were started by William Robinson and concretely embodied by Gertrude Jekyll. It is worth shedding new light on wild gardens, as they served as a pathbreaker for ecological design and an important foundation for the specialization of naturalism, which are part of the most important topics in modern gardens. This study aimed to infer the conceptual model of wild gardens and identify their historic significance by comparatively analyzing Robinson's Gravetye Manor and Jekyll's Munstead Wood. The results are: Firstly, they inherited inspirations for spatial organization from basic cottage gardens and introduced informal forms. Secondly, in terms of the use of materials, they had observed various climates in their journeys so that they could use both native and naturalized plants based on their understanding of the plants' hardiness and exotic species. They also displayed interests in plants in the woodlands and forests. Thirdly, in terms of design techniques, they investigated the colors and textures of individual plants and their relationships to produce a variety of views that resembled nature in microcosm. Fourthly, in terms of maintenance, their basic orientation was the minimum maintenance to allow plants to live according to their nature.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.43 no.6
• /
• pp.86-97
• /
• 2015

"The Jayeon Madang Development Project("JMDP")" is a project being promoted by the Ministry of Environment to create a cultural space and a natural rest area within the city. Abuksan, located at Abuksan in Gyeongsangnam-do Miryang-si Naeil-dong, has suffered a substantial amount of environmental degradation over time, so the need for ecological restoration made it a natural choice for the location of the JMDP's site. The purpose of this study is to examine ecological restoration design strategies used in Abuksan as part of the JMDP using system dynamics. The national archery center, hole, and arable land sites are key restoration areas in Abuksan that have faced with ecological problems. In this study, we identified the status of each site, determined key strategies being implemented, and designed based on the strategies implemented up to this point for solving problems associated with each sites through the use of causal loop diagrams. The results of the causal loop diagram analysis are as follows. The national archery center site was designed around strategies including planting green manure crops and introducing hugelkultur to reduce soil acidification and green network degradation. The hole site was designed as a constructed wetland based on the emergence of hygropreference vegetation, hydrated by rainwater collected at the bottom of hole, ecological and cultural benefits of such an environment. The arable land site restoration design was built around planting native vegetation on one part of the arable land site after soil quality improved and around restoration of grassland and a dry wetland on the other part of the site to reduce soil acidification, erosion, and green network degradation. This study is a significant attempt to apply principles of system dynamics to ecological restoration by providing the design strategies using comprehension of some problems in the ecosystem feedback loops, which has not been used before in general design processes for ecological restoration.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.6 no.2
• /
• pp.124-130
• /
• 1986

To find out the optimum split application ratios of fertilizer nitrogen and potassium in the newly opened pasture at spring, a field experiment was conducted. Two levels of nitrogen and potassium application and three split application ratios of both fertilizer were treated by split design with 4 replications. The experiment was carried out from March, 1984 to October, 1985 on the experimental field of the Livestock Experiment Station in Suweon. The results obtained are summarized as follow: 1. Total DM yield was not showed signigicant difference among split application ratios of nitrogen and potassium. 2. DM yield of grasses was 4,854 kg/ha in heavy basal application of N and $K_2$O (160 kgN: 140 kg $K_2$O/ha), it was significantly increased by 16% than of conventional dressing (80 kg N: 70 kg $K_2$O/ha). But there was almost no difference in dry matter among N and $K_2$O split application ratios. 3. Dry matter yield of legume in heavy basal dressing was decreased by 23% than that of conventional dressing. In the methods of NK application, dry matter yield of legume was tended to decrease by 3% with equal application and 9% with heavy dressing in autumn, when compared with that of heavy dressing in spring. 4. There was no significant difference in dry matter yield of native species between different basic fertilization levels, but dry matter yield of native species was decreased by 6% with equal application and more increased by 11% with heavy dressing in autumn, when compared with the yield of heavy dressing in spring. 5. In aspect of the botanical composition, the percentage distribution of grasses was increased and the ratio of legume was greatly decreased by heavy dressing at basal fertilization. Also for good maintenance of botanical composition, it is suggested that the equal split application of N and $K_2$O was desirable.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.9
• /
• pp.432-439
• /
• 2020

To improve the relatively low economic efficiency of the Korean native pig, the Korean National Institute of Animal Science developed a novel pig breed, the Woori black pig (W), by crossing Korean native and Duroc (D) pigs. This study was conducted to evaluate the effects of W as a terminal sire on growth performance, body shape, and retail cut yield of crossbred pigs. By using a completely randomized design, 32 crossbred pigs were allotted to one of two treatment groups based on terminal sire. The two groups were LYD [(Landrace × Yorkshire) × D sire] and LYW [(Landrace × Yorkshire) × W sire]. The experimental assessments were conducted over 53 days. The terminal sire breed had no significant effect on body weight (BW) at d 53, or on BW gain, average daily gain, or days to reach a 90 kg body weight. Moreover, there were no significant differences in body length, body height, or chest depth between the two groups. However, there was a significant difference (p < 0.05) in backfat thickness between the LYD (17.29 mm) and LYW (18.96 mm) groups. Loin yield of crossbred pigs in the LYW group (13.11%) was significantly lower (p < 0.05) than that in the LYD group (13.85%). By contrast, the Boston butt yield was significantly higher (p < 0.05) in the LYW group (8.99%) than in the LYD group (8.21%). In conclusion, these results suggest crossbred pigs sired by a Woori black pig had growth performance, shape, and retail cut yield (except loin yield) Ed. Note: I assume the lower loin yield is a negative factor so I included this wording. similar to those sired by a Duroc pig. The results showed no overall negative effect Ed. Note: I assume the lower loin yield is a negative factor so I used this wording. on crossbred offspring, indicating the suitability of the Woori black pig as a terminal sire.

• Journal of Animal Science and Technology
• /
• v.53 no.2
• /
• pp.161-169
• /
• 2011

Methane production during anaerobic fermentation in the rumen represents an energy loss to the host animal and induces emissions of greenhouse gases in the environment. Our study focused on comparison in methane production from growing Korean native steers fed different grain sources. Six Hanwoo steers (BW = $180.6{\pm}3.1$ kg) were fed, on a DM basis (TDN 2.80 kg), 40% timothy and 60% barley concentrate (Barley) or corn concentrate (Corn), respectively, based on the Korean Feeding Standards. Each period lasted 18 days including a 14-day adaptation and a 4-day measuring times. The steers were in the head hood chamber system (one cattle per chamber) during each measuring time to measure heat and methane production per day. Different grain sources did not affect digestibilities of dry matter, crude protein, crude fiber, crude fat, NDF, ADF and nitrogen-free extract. The mean methane concentrations per day were 202.0 and 177.1 ppm for Barley and Corn, respectively. Methane emission averaged 86.8 and 77.7 g/day for Barley and Corn, respectively. Methane emission factor by maintenance energy requirement for the growing steers fed barley based concentrate was higher than the steers fed corn based concentrate (Barley vs. Corn, 31.7 kg $CH_4\;head^{-1}\;yr^{-1}$ vs. 28.4 kg $CH_4\;head^{-1}\;yr^{-1}$). Thus, methane conversion rate was 0.065 (6.5%) and 0.055 (5.5%) for Barley and Corn, respectively.