• Title/Summary/Keyword: Native Breed

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Assessment of Genetic Diversity, Relationships and Structure among Korean Native Cattle Breeds Using Microsatellite Markers

  • Suh, Sangwon;Kim, Young-Sin;Cho, Chang-Yeon;Byun, Mi-Jeong;Choi, Seong-Bok;Ko, Yeoung-Gyu;Lee, Chang Woo;Jung, Kyoung-Sub;Bae, Kyoung Hun;Kim, Jae-Hwan
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.11
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    • pp.1548-1553
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    • 2014
  • Four Korean native cattle (KNC) breeds-Hanwoo, Chikso, Heugu, and Jeju black-are entered in the Domestic Animal Diversity Information System of the United Nations Food and Agriculture Organization (FAO). The objective of this study was to assess the genetic diversity, phylogenetic relationships and population structure of these KNC breeds (n = 120) and exotic breeds (Holstein and Charolais, n = 56). Thirty microsatellite loci recommended by the International Society for Animal Genetics/FAO were genotyped. These genotypes were used to determine the allele frequencies, allelic richness, heterozygosity and polymorphism information content per locus and breed. Genetic diversity was lower in Heugu and Jeju black breeds. Phylogenetic analysis, Factorial Correspondence Analysis and genetic clustering grouped each breed in its own cluster, which supported the genetic uniqueness of the KNC breeds. These results will be useful for conservation and management of KNC breeds as animal genetic resources.

Determination and comparison of growth performance parameters between two crossbred strains of Korean native chickens with a white semi broiler chicken for 84 days post-hatch

  • Nawarathne, Shan Randima;Lee, Soo Kee;Cho, Hyun Min;Wickramasuriya, Samiru Sudharaka;Hong, Jun Seon;Kim, Yu Bin;Heo, Jung Min;Yi, Young-Joo
    • Korean Journal of Agricultural Science
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    • v.47 no.2
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    • pp.255-262
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    • 2020
  • The aim of this study was to investigate the growth performance between two different crossbred strains of Korean native chickens denoted as 1E and 2E with a white semibroiler (LL) from hatch to d-84 post-hatch. A total of 450 one-day-old chicks were randomly distributed into cages which included 15 replicate cages each for 1E and 2E with 9 replicate cages for LL. A three phase feeding program was used as a starter (Day 1 - 35; crude protein [CP] 20.3%; metabolizable energy [ME] 3,059 kcal·kg-1), grower (Day 36 - 56; CP 18.6%; ME 3,123 kcal·kg-1) and finisher (Day 57 - 84; CP 16.7%; ME 3,187 kcal·kg-1). The results revealed that LL had a higher (p < 0.05) BW during d-8 to d-84 compared with the other two cross breed groups. A higher (p < 0.05) ADG was observed from the LL during the entire period except from d-1 to d-14 and d-71 to d-84 when compared with 1E and 2E. Shank length did not vary (p > 0.05) between the strains, despite LL having a SL that was 2.86% higher (p < 0.05) when compared with the other strains from d-15 to d-21 post-hatch, and 1E had a SL that was 2.28% (p < 0.05) higher when compared with the other strains during the first week of the experiment.

Production Traits and Stress Responses of Five Korean Native Chicken Breeds (한국토종닭 5품종의 생산능력 및 스트레스 반응 정도)

  • Cho, Eun Jung;Choi, Eun Sik;Jeong, Hyeon Cheol;Kim, Bo Kyung;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.47 no.2
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    • pp.95-105
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    • 2020
  • This study presents the production characteristics and physiological characteristics of five Korean native chicken (KNC) breeds consisting of Hwanggalsaek Jaeraejong (HJ), Korean Rhode Island Red (KR), Korean White Leghorn (KL), Korean Brown Cornish (KC), and Korean Ogye (KO). We investigated their production performances, vitalities, and stress responses. We measured the survival rate, body weight, age at first egg-laying, hen-day egg production, egg weight, amount of telomeric DNA, heterophil-lymphocyte ratio (H/L ratio), and heat shock protein (HSP)-70, HSP-90α and HSP-90β gene expression levels for 493 KNCs. The survival rate was highest in KR, and lowest in KO. Body weights were steadily high in the order of KC, KR, HJ, KO and KL. Average hen-day egg production was highest in KL, and lowest in KC. While the amount of telomeric DNA was highest in KR, and lowest in KC. Furthermore, both the H/L ratio and the HSP-90β gene expression level were highest in KC, and lowest in KR. These results indicated that the KR breed was highly resistant to stress, whereas KC was more susceptible to stress. Taken together, it is considered that with improvements the KC breed would be more suited to be used as a Korean broiler breed while KL would be more appropriately used as a Korean layer breed. In addition, it is considered that the KR breed is appropriate to be used as a maternal chicken breeder based on good production capacity and excellent robustness, while the HJ breed is desirable to be improved as a high-quality Korean meat breed based on its excellent meat quality.

Genetic Relationship among the Korean Native and Alien Horses Estimated by Microsatellite Polymorphism

  • Cho, G.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.6
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    • pp.784-788
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    • 2006
  • Microsatellite polymorphism and the genetic relationship were estimated using genotype information of 305 horses from 11 microsatellite loci. The breeds include the indigenous Korean breeds, Korean native horse (102) and Jeju racing horse (56) together with Japan Hokkaido horse (5), Mongolian horse (19), Thoroughbred horse (108), Quarter horse (11) and Przewalskii horse (4). Allelic frequencies, the number of alleles per locus were estimated by direct counting from observed genotype, and genetic variability was computed using the CERVUX software and DISPAN. The number of alleles per locus varied from 6 (HMS6) to 18 (ASB17) with an average value of 10.45 in horse breeds. The expected total heterozygosity ($H_T$) and coefficient of gene differentiation ($G_{ST}$) ranged 0.764-0.921 (the average value was 0.830) and 0.102-0.266 (the average value was 0.180) in horse breeds, respectively. Four populations (Przewalskii horse, Japan Hokkaido horse, Quarter horse, Thoroughbred horse) showed lower heterozygosity than the average value (the average value was 0.710). The expected heterozygosity within breed ($H_S$) and mean no. of observed alleles ranged from $0.636{\pm}0.064$ (Japan Hokkaido horse) to $0.809{\pm}0.019$ (Mongolian horse), and from 2.73 (Przewalskii horse) to 8.27 (Korean native horse), respectively. The polymorphic information content (PIC) ranged from 0.490 (Przewalskii horse) to 0.761 (Mongolian horse) with an average value of 0.637 in horse breeds. The results showed three distinct clusters with high bootstrap support: the Korean native horse cluster (Korean native horse, Mongolian horse), the European cluster (Przewalskii horse, Thoroughbred horse), and other horse cluster (Jeju racing horse, Japan Hokkaido horse, and Quarter horse). A relatively high bootstrap value was observed for the Korean native horse cluster and European cluster (87%), and the Korean native horse and Mongolian horse (82%). Microsatellite polymorphism data were shown to be useful for estimating the genetic relationship between Korean native horse and other horse breeds, and also be applied for parentage testing in those horse breeds.

Survey of pathogenic microorganisms contamination of chicken carcasses (도계육에서 병원성 미생물 오염도 조사)

  • Lee, Eun-Me;Shin, Dong-Su;Kwon, Mee-Soon;Lee, Sung-Jae
    • Korean Journal of Veterinary Service
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    • v.38 no.3
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    • pp.167-171
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    • 2015
  • Pathogenic microorganisms were monitored on the chicken carcasses in slaughterhouse of Jeonbuk area. It was conducted to evaluate the microbiological quality on 204 chicken carcasses. Staphylococcus (S.) aureus was isolated in largest number and its ration was 41.2%, Salmonella spp. 6.4%, Campylobacter (C.)jejuni 7.4%, C. coli 7.4%. Serotype of Salmonella (S.) spp. was identified as S. Infantis 46.1%, S. Enteritidis 23.1%, S. Typhimurium 7.7%, S. Montevideo 7.7%. In breed chickens, Salmonella spp. was detected broiler 4.1%, white semi-broiler 8.0% Korean native chicken 12.0%. C. jejuni was isolated broiler 7.4%, white semi-broiler 12.0%, Korean native chicken 0%, C. coli, broiler 7.4%, white semi-broiler 0%, Korean native chicken 18.1% and S. aureus, broiler 38.8%, white semi-broiler 40.0%, Korean native chicken 51.5%.

Analysis of Genetic Characteristics of Korean Native Chicken Using DNA Marker (DNA Marker를 이용한 한국 재래닭의 유전특성 분석)

  • 이학교;이성진;황규춘;정일정;박용호;손시환;신영수;오봉국;한재용
    • Korean Journal of Poultry Science
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    • v.23 no.4
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    • pp.177-183
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    • 1996
  • This study was conducted to analyze genetic characteristics of Korean Native Chicken three lines classified on the basis of the feather color and appearance (Red, Yellow, and Black) using DNA fingerprinting method. To estimate the genetic relatedness among breeds and similarities within breeds, we collected blood samples from Korean Native Chicken (KNC), Rhode Island Red (RIR), White Leghorn (WL), and Cornish(CN) and obtained genomic DNA from the blood of 10 individuals randomly selected within the breeds and lines. The genomic DNA samples were digested with restriction enzymes (Hinf J, Hae Ill) and hybridized with various probes (Jeffreys' probes 33.15, 33.6 and M13) after Southern transfer. Genetic similarities within breeds were characterized by band sharing (BS) value, estimated by the DFP band pattern between the pair of lanes. BS values within WL, RIR, and KNC were 0.82, 0.70 and 0.56, respectively. Relative genetic diversity (BS value) of KNC was higher than those two breeds (WL, RIR). Estimation of genetic similarity between KNC lines and control breed (RIR) was 0.32, whereas similarity within KNC lines (6 groups) was 0.50. In this analysis, KNC was showed to have a highly genetic diver-sity at the DNA level, and to be closer in genetic distance to RIR (0.67) than any other breeds.

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Quality Characteristics of Korean Native Chicken Meat (한국산 토종 닭고기의 품질 특성)

  • 권연주;여정수;성삼경
    • Korean Journal of Poultry Science
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    • v.22 no.4
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    • pp.223-223
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    • 1995
  • A study was conducted to compare the quality characteristics among commercial broiler, Wangchoo (imported dual purpose breed) and Korean native chicken(KNC). Thigh and breast meat of the broiler(7-wk old), Wangchoo(15-wk old), and Korean native chicken(15-wk old) stored for 24 h at 5t were used to analyze chemical composition, physico-chemical characteristics, textural traits and sensory evaluation test. Crude fat and moisture contents in broiler meat and crude protein content in KNC were significantly(P<.05) higher than those in the other breeds regardless of parts of the body. Total collagen content in broiler meat was significantly higher than those of the other breeds, however, the heat-soluble and the acid-soluble collagen content in Wangchoo were significantly lower than those of the other breeds. Water-holding capacities of KNC in breast meat, and of broiler in leg meat were significantly higher than that of the other breeds, while the results of the water-holding capacity and the cooking loss were reversed. Myofibrillar fragmentation index in broiler meat was significantly higher than that in the other breeds regardless of body parts. Hardness, elasticity and cohesiveness in Wangchoo were significantly higher than those in the other breeds. The prominent fatty acids were oleic, palmitic and linoleic acids and run up to 79.03~83.82 %, regardless of breeds and parts. The sensory evaluation score of tenderness, taste and preference in Wangchoo were lower compared to the broiler and KNC, however, they were not significantly different between broiler and KNC. In conclusion, the quality characteristics of KNC were excellent compared to Wangchoo.

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Comparison of Superoxide Dismutase and Peroxidase Activities in Rice Varieties

  • Chung, Ill-Min;Kim, Kwang-Ho;Ahn, Joung-Kuk;Lee, Jin-Ohk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.277-281
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    • 2000
  • Fifty-four Korean native and 28 foreign varieties harvested in 1998 and 1999 were examined for superoxide dismutase (SOD) and peroxidase (POD) activities. The SOD and POD activities of leaves extracts in Korean native and foreign rice varieties showed variation at the heading stage. The activities of SOD and POD changed with growth stage. In comparison of storage duration, the SOD and POD activities of the extract from three months stored seeds in Korean native (CV=53.3%) and foreign rice (CV=57.9%) varieties were higher than that of stored rices for a year in seed extracts although the activities among varieties did not show significant variation. Also, the averaged activity of foreign rice varieties (SOD=12.9%) was relatively higher than that of korean native rice varieties (SOD=10.7%). The test of activity at the enzymatic level related to antioxidative activity suggests that the rice varieties with higher antioxidative potentials can be developed and also may provide information with rice breeder to breed rice variety with a high antioxidative activity.

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Investigation of single nucleotide polymorphism in TSH-β and CaSR associated with body weight in Korean native chickens (Gray Brown)

  • Oh, Dongyep;Ha, Jae Jung;Yi, Jun Koo;Kim, Dae Hyun;Oh, Seung Min;Kim, Songmi;Han, Kyudong;Park, Yong-Soo
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.3
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    • pp.129-136
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    • 2021
  • This study identified single nucleotide polymorphisms (SNPs) that affect the body weight of chickens. Analysis of body weight showed that the Cornish breed had the highest body weight, and the Korean native chicken (Gray Brown) had the lowest body weight. TSH is composed of an α-subunit and a β-subunit, and the TSH-β gene encoding the β-subunit has been reported to be associated with obesity. In chickens, it is located on chromosome 26 and is reported to be associated with growth. The calcium-sensing receptor gene (CaSR) plays a role in the regulation of extracellular calcium homeostasis and is responsible for calcium absorption in the urinary tract, which affects the eggshell quality in poultry. It was shown that TSH-β was strongly correlated with weight in Cornish and Korean native (Gray Brown) chickens, particularly in those with the CC trait. However, CaSR showed no association with body weight in poultry; it was associated with calcium and the eggshell. Thus, selection for TSH-β can be used to produce individuals with more favorable traits in terms of body weight.

Assessment of Breed- and Sex-based Variation in Flavor-related Compounds of Duck Meat in Korea

  • Lee, Hyun Jung;Kim, Hyun Joo;Yong, Hae In;Khan, Muhammad I.;Heo, Kang Nyung;Jo, Cheorun
    • Korean Journal of Poultry Science
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    • v.42 no.1
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    • pp.41-50
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    • 2015
  • The objective of present research is to evaluate the effect of breed and sex on flavor-related compounds of duck meat in Korea. Breast meat of each different breed [Korean native duck (KND) and commercial duck, Cherry Valley (CD)] and sex (male and female) were analyzed for chemical composition, nucleotide, fatty acid composition, and free amino acid. In comparison within the different breed and sex, CD and female duck were higher in moisture composition compared to KND and male duck. The meat from female duck had higher inosine monophosphate (IMP) contents while the meat from KND had higher inosine contents (P<0.05). Mostly, male duck had higher contents of free amino acid, including alanine, aspartic acid, glycine, histidine, leucine, serine, valine, glutamic acid and cysteine (P<0.05), even though no significance in the sum of taste-related free amino acid was observed with respect to breed and sex. From the results, it was found that breed has no significant impact while sex has influenced the flavor-related compounds, especially, IMP and most of the free amino acids. In order to get breed-specific advantages, KND should be developed based on flavor aspect and further studies using KND with known genetic confirmation should be conducted for the extrinsic and intrinsic flavor influencing factors.