• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.26-32
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• 1998

Three major trails of campus forest in Chungnam National University were selected to investigate the use impacts on environmental deterioration of trail according to the different amount of use. Rook-exposed, root-exposed, deepening, widening, diverged points as the deterioration types of trail which were surveyed at total of 92 points in major trail of campus forest in Chungnam National University. Major deterioration types of trail were widening, rock-exposure, root-exposure, in order of frequency. And trail conditions (trail slope and maximum depth) of deteriorated points were significantly different from those of non-deteriorated points.

• Journal of the Korean Institute of Landscape Architecture
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• v.36 no.5
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• pp.35-41
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• 2008

The purpose of this study is to determine those factors critical in the determining of regional trail routes. Initially, a consideration of the concept of the regional trail was conducted through literature reviews and 13 planning elements were determined from previous studies related to trail planning and trail construction studies. An additional 13 items were taken from literature research related to nature experiences or were sourced by interviewing experts. To verify these planning elements, a survey of experts was conducted. Individual elements were assessed concerning their degree of importance. As a result of this survey, it was found that the protection of ecologically sensitive areas and the use of existing trail routes are important route planning factors. These planning factors can be used for making decisions regarding regional trail route directions and situations.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1363-1369
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• 2013

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis by targeting cancer cells. However, some cancer cells are resistant to TRAIL-induced cytotoxicity. One method of overcoming TRAIL resistance is combination treatment with reagents to sensitize cells to TRAIL. Luteolin, a flavonoid, has been shown to have anti-cancer effects by inducing apoptosis and cell cycle arrest in various cancer cell lines in vitro. In this study, we investigated the effects of combination treatment with non-toxic concentration of TRAIL and luteolin in T24 human bladder cancer cells. Combined treatment with luteolin and TRAIL significantly inhibits cell proliferation via activation of caspases by inducing Bid truncation, up-regulation of Bax and down-regulation of X-linked inhibitor of apoptosis protein (XIAP). However, the apoptotic effects of combination treatment with luteolin and TRAIL were significantly inhibited by specific caspases inhibitors. Taken together, these results indicate that combination treatment with TRAIL and luteolin can induce apoptosis in TRAIL-resistant cancer cells through down-regulation of XIAP and modulation of tBid and Bax expression.

• Biomolecules & Therapeutics
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• v.23 no.1
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• pp.31-38
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• 2015

Histone acetylation plays a critical role in the regulation of transcription by altering the structure of chromatin, and it may influence the resistance of some tumor cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) by regulating the gene expression of components of the TRAIL signaling pathway. In this study, we investigated the effects and molecular mechanisms of trichostatin A (TSA), a histone deacetylase inhibitor, in sensitizing TRAIL-induced apoptosis in Caki human renal carcinoma cells. Our results indicate that nontoxic concentrations of TSA substantially enhance TRAIL-induced apoptosis compared with treatment with either agent alone. Cotreatment with TSA and TRAIL effectively induced cleavage of Bid and loss of mitochondrial membrane potential (MMP), which was associated with the activation of caspases (-3, -8, and -9) and degradation of poly (ADP-ribose) polymerase (PARP), contributing toward the sensitization to TRAIL. Combined treatment with TSA and TRAIL significantly reduced the levels of the cellular Fas-associated death domain (FADD)-like interleukin-$1{\beta}$-converting enzyme (FLICE) inhibitory protein (c-FLIP), whereas those of death receptor (DR) 4, DR5, and FADD remained unchanged. The synergistic effect of TAS and TRAIL was perfectly attenuated in c-$FLIP_L$-overexpressing Caki cells. Taken together, the present study demonstrates that down-regulation of c-FLIP contributes to TSA-facilitated TRAIL-induced apoptosis, amplifying the death receptor, as well as mitochondria-mediated apoptotic signaling pathways.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.230-247
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• 2023

This study was conducted on migrants in 5 cities and counties near the Jirisan Dulle-gil Trail, designated as a National Forest Trail, to find out how the National Forest Trail affects the quality of life after migrants from urban to mountain villages. The group that used the Jirisan Dulle-gil Trail before and/or after the migration showed higher levels of impact on the migration decision, life satisfaction, and behavioral intention than the group that did not use the trail. The group that was affected by the Jirisan Dulle-gil Trail in deciding on the migration also showed higher usage satisfaction with the Jirisan Dulle-gil Trail, life satisfaction, satisfaction with the migration, and behavioral intention than the unaffected group. There were also significant differences in the quality of life according to the migration area, location satisfaction among the migration satisfaction levels, and behavioral intention. In conclusion, it was confirmed that the Jirisan Dulle-gil Trail plays an important role in the decision to migrate to mountain villages and the quality of life after the migration. The results of this study are expected to be used as basic data to present policies related to National Forest Trails that can contribute to the development of mountain villages and countermeasures against population extinction in mountain villages.

• Korean Journal of Environment and Ecology
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• v.26 no.5
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• pp.827-834
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• 2012

The purpose of this study is to provide scientific data to support policy making on core area management in national parks, particularly to resolve conflict regarding trail closure, by analyzing the physical characteristics of trails in the Seoraksan and Songnisan National Park on the Baekdudaegan mountains. For the analysis, we surveyed 112 points selected from three sections and one section of closed trails in Seoraksan and Songnisan, respectively (17.1 km in total). The surveyed trails had, on average, trail width of 0.98m, baresoil width of 0.84 m, maximum erosion depth of 11.6cm, and trail slope of 14.2%. Of 53 out of 112 surveyed points (47.3%), we found exposed roots. Only 47 points (42.0%) did not show any type of physical deterioration. The magnitudes of deterioration in the surveyed closed-trail are relatively lower than those in open-trail in other national parks and are similar to those of ridge trails on the Baekdudaegan mountains.

• Journal of the Korean Geographical Society
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• v.46 no.4
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• pp.425-441
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• 2011

Ridge traversing trail in Mount Jiri National Park is classified as flat, gully-like, unilateral, and asymmetric bilateral, paths based on a location and gradient of paths. These types are interchangeable due to a drainage condition of trail surfaces. Using a rapid survey, the trail is 135.9 cm wide, 23.6 cm deep and $5.1^{\circ}$ in a gradient, respectively. All treads have been compacted due to human trampling. The path width is affected by a slope aspect and a distribution of Sasa borealis. An asymmetric path is wider than a symmetric path. A soil erosion rate is equivalent to $68.9cm^2/year$ for the period from 1960 to 2009, suggesting that the trail has been widened 2.7 cm/year and the tread lowered 0.4 cm/year. Trampling and needle ice action combined with rainwash induced by a pipeflow are dominant erosion processes contributing to the trail expansion.

• Korean Journal of Environment and Ecology
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• v.1 no.1
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• pp.35-45
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• 1987

To survey trail damage and vegetational change around trail at Bukhan Mountain National Park in Korea, field survey was executed over Bukhan mountain district during August, 1987. Trail damage was surveyed for he section of 7.18km from Ui valley to Jeongnung valley in which user's density was high. Ground vegetation was surveyed with a belt-transect method from trail edge to forest and edge species were surveyed with a belt method along trail edge. Interrelation between trail damage and user's density was not dear. Damage class II of trail showed 23.9% of the surveyed section and class III showed 8.0% and class II and III of natural trail showed 19.7% and those of facilitated trail showed 12.3%. The length of damage class II and III requiring readjustment were estimated as 3.65 km and 1.22km from the main trail course of 15.3km at Bukhan mountain district. In case of no intervention to forest by users, vegetational change around trails was appeared up to 6-8 m from trailside. But in case of intervention to forest by users, vegetational change was not coincident with the change of soil hardness and was diverse locally. Quercus mongolica, Lespedeza cyrtobotrya and Rbo-dodendron mucronulatum were appeared as Raunkiaer frequency class E, and Weigela subsessilis. Lespedeza maximowiczii, Rhododendron schlippenbachii and Sorbus alnifolid were appeared as class D at trail edge of ridge-Quercus mongolica was appeared as class E and Rhododenderon mucrounulatium. Stephanandra incisa were appeared as class D at trail edge of midslope. Rhododenron mucronulatum. Lespedeza maximowiczii and Stepanandra incisa were appeared as class D at trail edge of valley. Lespedeza cyrtobotrya. Lespedeza maximowiczii and Stephanandra incisa were appeared as class D at trail edge of valley beside motorway.

• Tuberculosis and Respiratory Diseases
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• v.63 no.1
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• pp.42-51
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• 2007

Background: TRAIL is a cytokine that selectively induces apoptosis in various cancer cell lines. Gefitinib is new targeted drug applied in lung cancer that selectively inhibits EGFR tyrosine kinase. However, lung cancers have shown an initial or acquired resistance to these drugs. This study examined the effect of IGF-1R and its blockade on enhancing the sensitivity of lung cancer cell lines to TRAIL and gefitinib. Methods: Two lung cancer cell lines were used in this study. NCI H460 is very sensitive to TRAIL and gefitinib. On the other hand, A549 shows moderate resistance to TRAIL and gefitinib. The IGF-1R blockade was performed using adenoviruses expressing the dominant negative IGF-1R and shRNA to IGF-1R and AG1024 (IGF-1R tyrosine kinase inhibitor). Results: The adenovirus expressing dominant negative IGF-1R(950st) induced the increased expression of defective IGF-1R on the lung cancer cell surface, and the adenovirus-shIGF-1R effectively decreased the level of IGF-1R expression on cell surface. The genetic blockade of IGF-1R by the adenovirus-dnIGF-1R and AG1024 increased the sensitivity of A549 cells to TRAIL. The reduction of IGF-1R by transduction with ad-shIGF-1R also increased the sensitivity of the A549 cells to gefitinib. Conclusion: The blockade of IGF-1R through various mechanisms increased the sensitivity of the lung cancer cell line that was resistant to TRAIL and gefitinib. However, further studies using other cell lines showing acquired resistance as well as in vivo animal experiments will be needed.

• Journal of Life Science
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• v.26 no.4
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• pp.431-438
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• 2016

The tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is considered a promising anticancer agent due to its unique ability to induce cancer cell death having only negligible effects on normal cells. However, many cancer cells tend to be resistant to TRAIL. In this study, we investigated the effects and molecular mechanisms of sodium butyrate (SB), a histone deacetylase inhibitor, in sensitizing TRAIL-induced apoptosis in 5637 human bladder cancer cells. Our results indicated that co-treatment with SB and TRAIL significantly increased the apoptosis induction, compared with treatment with either agent alone. Co-treatment with SB and TRAIL effectively increased the cell-surface expression of death receptor (DR) 5, but not DR4, which was associated with the inhibition of cellular Fas-associated death domain (FADD)-like interleukin-1β-converting enzyme (FLICE) inhibitory protein (c-FLIP). Furthermore, the activation of caspases (caspase-3, -8 and -9) and degradation of poly(ADP-ribose) were markedly increased in 5637 cells co-treated with SB and TRAIL; however, the synergistic effect was perfectly attenuated by caspase inhibitors. We also found that combined treatment with SB and TRAIL effectively induced the expression of pro-apoptotic Bax, cytosolic cytochrome c and cleave Bid to truncated Bid (tBid), along with down-regulation of anti-apoptotic Bcl-xL expression. These results collectively suggest that a combined regimen of SB plus TRAIL may offer an effective therapeutic strategy for safely and selectively treating TRAIL-resistant bladder cancer cells.