• Title/Summary/Keyword: National labs

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Diversity of Lactic Acid Bacteria (LAB) in Makgeolli and Their Production of γ-Aminobutyric Acid (막걸리에서 분리한 Lactic Acid Bacteria (LAB)의 다양성 분석과 γ-aminobutyric acid 생산능 연구)

  • Lee, Hye-Lim;Kang, Ki-Won;Seo, Dong-Ho;Jung, Jong-Hyun;Jung, Dong-Hyun;Kim, Gye-Won;Park, Sun-Young;Shin, Woo-Chang;Shim, Hyoung-Seok;Park, Cheon-Seok
    • Korean Journal of Food Science and Technology
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    • v.47 no.2
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    • pp.204-210
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    • 2015
  • Makgeolli is made from rice or flour, yeast, and nuruk, a fermentation starter. The flavor of makgeolli is affected by sugars, amino acids, organic acids and volatile flavor compounds produced by various microorganisms. In this study, lactic acid bacteria (LAB) were isolated from unsterilized makgeolli samples collected from several provinces in Korea, and then later identified. Under anaerobic conditions, LAB density ranged from $5.0{\times}10^6$ to $1.5{\times}10^8CFU/mL$; yeast density ranged from $2.5{\times}10^7$ to $1.5{\times}10^8CFU/mL$. Of the LAB isolated from makgeolli, 1,126 were analyzed using restriction fragment length polymorphism (PCR-RFLP) analysis of 16S rRNA, which allowed for classification into five groups. Of the 1,126 LABs tested, 130 produced ${\gamma}$-aminobutyric acid (GABA).

Transition of Teachers' Perception and Improvement of Students' Perception on Food Additives through a Training Program (식품첨가물 바르게 알기 연수를 통한 교사들의 인식 전환과 학생들의 인식 개선 효과)

  • Kim, Jeong-Weon
    • Journal of Food Hygiene and Safety
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    • v.32 no.2
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    • pp.101-106
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    • 2017
  • Based on the previous reports that majority of teachers have negative perceptions on food additives, a teacher training program called 'Let's teach food additives correctly' was developed and applied to improve teachers' unbalanced perception on food additives and let them teach students with sound scientific background. The 15-hours training program consisted of understanding of food safety and food additives, education materials on food additives, development of teaching-learning plan, meeting with professionals from Ministry of Food and Drug Safety (MFDS), visiting MFDS labs, application to school class, and evaluation. A total of 63 teachers participated in the training through 2 sessions conducted in August 2016. As a result, teachers showed high satisfaction rates (4.2~4.5 in 5.0-Likert scale) and 91.5% answered the training helpful for the school class. Although their initial intention to participate the training was to know the details of negative intake effects of food additives, their such perception was totally changed in addition, they suggested a continuous training for teachers and immediate correction of incorrect information in school textbooks. Also, post-training education for 1,172 students by these teachers appeared to improve the understanding of and the native perceptions on food additives significantly (p < 0.001). Above results showed that the training program could solve the problem of transmitting unbalanced information on food additives to students by training teachers, and such channel could be used to facilitate food-related risk communication.

A Comparative Study of Production of [68Ga]PSMA-11 with or without Cassette Type Modules (비 카세트 방식과 카세트 방식을 이용한 [68Ga]PSMA-11의 자동 합성 방법 비교)

  • Hyun-Sik, Park;Byeong-Min, Jo;Hyun-Ho, An;Hong-Jin, Lee;Jin-Hyeong, Lee;Gyeong-Jae, Lee;Byung-Chul, Lee;Won-Woo, Lee
    • The Korean Journal of Nuclear Medicine Technology
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    • v.26 no.2
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    • pp.15-19
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    • 2022
  • Purpose [68Ga]PSMA-11 is needed the high reproducibility, excellent radiochemical yield and purity. In term of radiation safety, the radiation exposure of operator for its production also should be considered. In this work, we performed a comparative study for the fully automated synthesis of [68Ga]PSMA-11 between non-cassette type and cassette type. Materials and Methods Two different type of modules (TRACERlab FX N pro for non-cassette type and BIKBox for cassette type) were used for the automated production of [68Ga]PSMA-11. According to the previously identified elution profile, Only 2.5 ml with high radioactivity was used for the reaction. After adjusting the pH of the reaction solution with HEPES buffer solution, the precursor was added and reacted with at 95 ℃ for 15 minutes. The reaction mixture was separated and purified using a C18 light cartridge. The product was eluted with 50% EtOH/saline solution and diluted with saline. It was completed by sterilizing filter. In the non-cassette type, the aforementioned process must be prepared directly. However, in the cassette method, synthesis was possible simply by installing a kit that was already completed. Results Both total [68Ga]PSMA-11 production time were 25±3(non-cassette type) and 23±3 minutes(cassette type). The radiochemical yield of the non-cassette type(65.5±5.7%) was higher than that of the cassette type(61.6±4.8%) after sterilization filter. The non-cassette type took about 120 minutes of preparation time before synthesis due to washing of synthesizer and reagent preparation. However, since the cassette type does not require washing and reagent preparation, it took about 20 minutes to prepare before synthesis. Both type of synthesizer had a radiochemical high purity(>99%). Conclusion The non-cassette type production of [68Ga]PSMA-11 showed higher radiochemical yield and lower cost than the cassette type. However, The cassette type has an advantage in terms of preparation time, convenience, and equipment maintenance.

Characteristics of Exposure to Humidifier Disinfectant by Lung Injury Patients (가습기 살균제 폐 손상 피해자의 살균제 노출 특성 -태아와 임산부 노출을 중심으로 -)

  • Park, Dong-Uk;Ryu, Seung-Hun;Lim, Heung-Kyu;Kim, Sun-Kyung;Ahn, Jongju;Roh, Hyun-Suk;Choi, Ye-Yong;Cha, Won-Seok;Lee, Eun;Hong, Sang-Bum;Do, Kyung-Hyun;Cho, Jae-lim;Bae, Mun-Joo;Shin, Dong-Chun;Paek, Domyung;Hong, Soo-Jong
    • Journal of Environmental Health Sciences
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    • v.42 no.3
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    • pp.147-159
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    • 2016
  • In South Korea, many cases of humidifier disinfectant-associated lung injury (HDLI) have been reported among people who used humidifier products containing humidifier disinfectant (HD). The objective of this study is to characterize exposure to HD among a total of 221 HDLI patients who used HD. Information and data on the HDs used were collected through a structured questionnaire and home environmental investigations. The conditions of these 221 HDLI patients were clinically confirmed to be caused by the use of HD. Children aged under 5 years old made up the highest proportion of HDLI cases (n=125, 56.6 %), followed by pregnant women (n=35, 15.8%). Forty-three percent (n=95) of the victims died. There were three cases of fetuses and 35 pregnant women among the victims. The number of HDLI patients who used only the Oxy Saksak brand of HD was found to be 85 (38.5%), followed by the HD brands Cefu (n=24, 10.9%), Lottemart Wiselect (n=9, 4.1%) and Aekyung (n=3). Patients who exclusively used HD brands containing polyhexamethylene guanidine phosphate (PHMG) (n=13, 55.7%) as an active ingredient made up the largest share, followed by those who exclusively used HD containing only oligo(2-(2-ethoxy) ethoxyethyl guanidinium (PGH) (n=24, 10.9%) and by those who only used a mixture of chloromethylisothiazolinone (CMIT) and methylisothiazolinone (MIT) (n=3, 1.4%). HD products containing PHMG were found to be the most commonly used among the confirmed HDLI patients. Three exposed fetuses who never used HD after birth developed lung injuries, indicating a probability of exposure to HD during gestation. All HDLI patients responded that they used HD while sleeping and for longer than 10 hours per day. In conclusion, the development of HDLI was clinically found to be associated with the use of several HD products containing PHMG, PGH and CMIT/MIT.

The Effects of Supplemental Bacterial Phytase to the Calcium and Nonphosphorus Levels in Feed of Laying Hens (산란계 사료 내 칼슘 및 무기태 인 수준에 따른 Bacterial Phytase 급여 효과)

  • Kang, H.K.;Park, S.Y.;Yu, D.J.;Kim, J.H.;Kang, G.H.;Na, J.C.;Kim, D.W.;Suh, O.S.;Lee, S.J.;Lee, W.J.;Kim, S.H.
    • Korean Journal of Poultry Science
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    • v.35 no.2
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    • pp.143-151
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    • 2008
  • This study was conducted to identify the correlation of bacterial phytase ($Transphos^{(R)}$) to the calcium level in feed. Of all 21-week-old 720 HyLine brown laying hens, 2 birds of similar weight were placed on each individual cage. The experiment was conducted by $3{\times}2{\times}3$ factorial design with including 3 different levels of phytase (0, 300, and 1,000 DPU/kg), 2 different levels of calcium (3.5% and 4.0%), and 3 different levels of no NPP addition 0% (0.095 NPP), 0.5% (0.185% NPP), and 1.0% (0.275% NPP). The feeding trial maintained the ME level of 2,800 kcal/kg and 16% for crude protein. The diet was fed ad libitum and 17 hours of lighting was provided throughout the experimental period. Egg production seemed to increase, in the 300 DPU of bacterial phytase added group and the cracked egg tended to reduce in Transphos added group. The egg productivity between treatment groups did not show significant difference by dietary calcium level, whereas non NPP added group (0.095% NPP) was found to be low compared to NPP added groups (P<0.05). The highest mean egg weight and the highest daily egg mass were detected in 300 DPU phytase added group. Although the mean egg weight was significantly higher in treatment groups fed with 3.5% calcium containing feeds (P<0.05), daily egg mass was no among treatment groups. The mean egg weight and daily egg mass were the lowest in non NPP added group (0.095% NPP) compared to other treatment groups (P<0.05). The feed intake showed similar pattern regardless of the bacterial phytase and calcium levels in the diet. However, the treatment groups fed diets containing NPP level of 0.275% and 0.165% showed significantly higher feed intake than the group fed with 0.095% NPP (P<0.05). Although the feed conversion was not affected by calcium and NPP levels in the diet, the most improved result was obtained from 300 DPU phytase added group (P<0.05). The eggshell breaking strength and thickness increased as dietary calcium level increase the level of calcium increases in diet. The treatment groups fed diet containing 0.275% and 0.165% NPP revealed to show improvement in eggshell breaking strength and yolk color index compared to the NPP non added (0.095% NPP) treatment group. The result of the present study suggests that the appropriate level of microbial phytase is 300 DPU and at this level, tricalciumphosphate supplementation in feed can be reduced to 40% of NRC recommendation. Higher calcium level in feed fail to show synergistic effect by adding microbial phytase.

A Comparative Study on Recognition of Home Economics Curriculum between Alternative and General School Students - Middle Schools in Gyeonggi Province - (대안학교와 일반학교 학생들의 가정교과 인식에 관한 비교 연구 - 경기지역 중학교를 중심으로 -)

  • Ha, Yunmyoung;Lee, Jongyi;Lee, Joonho
    • Journal of Korean Home Economics Education Association
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    • v.24 no.4
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    • pp.39-58
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    • 2012
  • This study was practiced to compare educational status and recognition of Home Economics curriculum between alternative and general middle school students. The survey was conducted to 130 alternative school students and 241 general school students in Gyeonggi province. In students' satisfaction on their school, there is appeared the highest at 'average'(38.6%) in general schools and 'satisfied'(40.8%) in alternative schools, showing that those in alternative schools have greater satisfaction on their schools(p<0.001). In the degree of recognition on Home Economics curriculum, the perception as an 'important subject' was average of 3.08/5 points in general school and 3.32/5 points in alternative school, indicating that the recognition in alternative schools was higher than general ones(p<0.05). Also, degree of satisfaction on practice and lecture class was higher in alternative than general schools. However, it was found that the use of audiovisual learning material in alternative schools was much smaller than that of general ones, and the former had poor facilities and practice labs. Regarding degrees of interest in Home Economics curriculum, 'average'(36.9%) in general school and 'rather interested in the subject'(38.5%) in alternative schools were most common. About the opinion that they needed to learn Home Economics subject, the answer 'it is needed' was 67.6% in general schools and 79.2% in alternative ones, presenting that the students in alternative schools more felt the need to learn the subject(p<0.05). Regarding the comparison of interest level for each area in Home Economics curriculum according to gender, there was only difference on the area of 'preparation and management for clothing'. On the area, the degree of interest was higher in girls than boys at all the schools(p<0.05). Therefore, in alternative schools, it is suggested that various uses of audiovisual learning materials at teaching and expansion of practice facilities should be provided and created desirable Home Economics class. Also in general schools, it is urgent that countermeasures to increase the practice classes are established in order to improve interest and satisfaction of Home Economics education.

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A Study on the Development and Physical Properties of Low Yellowing Epoxy for Ceramic Preservation (도자기 복원용 저황변 Epoxy 수지의 개발 및 물성에 대한 연구)

  • Kim, Woo Hyun;Cheong, Da Som;Bae, Jin Soo;Jee, Joo Yeon;Wi, Koang Chul
    • Journal of Conservation Science
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    • v.30 no.2
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    • pp.181-187
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    • 2014
  • AThe studies on ceramic preservation have been conducted widely in various institutions such as national/ public museums and research labs as well as the excavation institutions and university museums. Although there are some differences in preservation methodologies and materials used across the institutions, the variation is minimal. Specifically, epoxy resin is mostly used for ceramic restoration for its high cohesiveness, low contraction and high strength although there are some variations in for Ceramic Preservation. The synthetic resin type used according to the type of damage in the ceramic. However, the yellowing or the change of color across the time after the restoration is the weakness of epoxy resin. In this study, we aim to develop a material which minimizes this yellowing of epoxy resin while enhancing its cohesiveness and strength as well as other physical properties. We made the new material to have similar properties with those used widely for the ceramic restoration, such as EPO-TEK301$^{(R)}$, L30$^{(R)}$, XTR-311$^{(R)}$ through comparative experiments. The cohesiveness of the newly developed resin was improved to 2.51(MPa), which is similar level of XTR-311$^{(R)}$ of the 2.30(MPa) but about 2x higher than the other resins EPO-TEK301$^{(R)}$, L30$^{(R)}$ (1.21 and 1.81 (MPa), respectively). Especially, the experiment on yellowing shows that the existing resins show the range of color change at 10~25(${\Delta}E^*ab$), but the new low yellowing epoxy resin has the color change value at 8.3 (${\Delta}E^*ab$), the value lowering the yellowing effect to 1 to 3 times of the existing epoxy resin, thereby solving the issue of generating sense of differences due to change of color or yellowing.