• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.111-111
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2007.04a
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• pp.76.1-76.1
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• 2007

• Food Science of Animal Resources
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• v.39 no.4
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• pp.601-609
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• 2019

Bifidobacterium longum KACC 91563 secretes family 5 extracellular solute-binding protein via extracellular vesicle. In our previous work, it was demonstrated that the protein effectively alleviated food allergy symptoms via mast cell specific apoptosis, and it has revealed a therapeutic potential of this protein in allergy treatment. In the present study, we cloned the gene encoding extracellular solute-binding protein of the strain into the histidine-tagged pET-28a(+) vector and transformed the resulting plasmid into the Escherichia coli strain BL21 (DE3). The histidine-tagged extracellular solute-binding protein expressed in the transformed cells was purified using Ni-NTA affinity column. To enhance the efficiency of the protein purification, three parameters were optimized; the host bacterial strain, the culturing and induction temperature, and the purification protocol. After the process, two liters of transformed culture produced 7.15 mg of the recombinant proteins. This is the first study describing the production of extracellular solute-binding protein of probiotic bacteria. Establishment of large-scale production strategy for the protein will further contribute to the development of functional foods and potential alternative treatments for allergies.

• Atmosphere
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• v.32 no.2
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• pp.87-101
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• 2022

In this paper, the performance improvement for the new KMA's Climate Prediction System (GloSea6), which has been built and tested in 2021, is presented by assessing the bias distribution of basic variables from 24 years of GloSea6 hindcasts. Along with the upgrade from GloSea5 to GloSea6, the performance of GloSea6 can be regarded as notable in many respects: improvements in (i) negative bias of geopotential height over the tropical and mid-latitude troposphere and over polar stratosphere in boreal summer; (ii) cold bias of tropospheric temperature; (iii) underestimation of mid-latitude jets; (iv) dry bias in the lower troposphere; (v) cold tongue bias in the equatorial SST and the warm bias of Southern Ocean, suggesting the potential of improvements to the major climate variability in GloSea6. The warm surface temperature in the northern hemisphere continent in summer is eliminated by using CDF-matched soil-moisture initials. However, the cold bias in high latitude snow-covered area in winter still needs to be improved in the future. The intensification of the westerly winds of the summer Asian monsoon and the weakening of the northwest Pacific high, which are considered to be major errors in the GloSea system, had not been significantly improved. However, both the use of increased number of ensembles and the initial conditions at the closest initial dates reveals possibility to improve these biases. It is also noted that the effect of ensemble expansion mainly contributes to the improvement of annual variability over high latitudes and polar regions.

• Journal of Microbiology
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• v.40 no.3
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• pp.230-233
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• 2002

Effects of short-chain primary amines on Aspergillus nidulans development were analyzed. Propylamine induced asexual development and inhibited sexual development. Even on medium containing lactose as the sole carbon source, on which little conidial heads are formed and sexual structures are formed preferentially, or when sexual development was induced, propylamine induced asexual development and inhibited sexual development. These effects of propylamine seemed to be due to accumulation of mRNA of the brlA gene, which has been identified as a positive regulator of asexual development, and due to the reduction of the veA mRNA level. The veA gene has been identified as an activator of sexual development and also as an inhibitor of asexual development. Other primary amines, methylamine and ethylamine, showed identical effects on development where short-chain primary amino also promoted asexual development and inhibited sexual development.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.9
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• pp.1328-1335
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• 2014

Significant SNPs associated with Warner-Bratzler (WB) shear force and sensory traits were confirmed for Hanwoo beef (Korean cattle). A Bonferroni-corrected genome-wide significant association (p< $1.3{\times}10^{-6}$) was detected with only one single nucleotide polymorphism (SNP) on chromosome 5 for WB shear force. A slightly higher number of SNPs was significantly (p<0.001) associated with WB shear force than with other sensory traits. Further, 50, 25, 29, and 34 SNPs were significantly associated with WB shear force, tenderness, juiciness, and flavor likeness, respectively. The SNPs between p = 0.001 and p = 0.0001 thresholds explained 3% to 9% of the phenotypic variance, while the most significant SNPs accounted for 7% to 12% of the phenotypic variance. In conclusion, because WB shear force and sensory evaluation were moderately affected by a few loci and minimally affected by other loci, further studies are required by using a large sample size and high marker density.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.305-305
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• 2022

In this study, 139 Korean landrace sorghum germplasms collected in 10 different Korean regions were analyzed for tannin, total phenolic contents(TPC), and antioxidant activities using three assays including ABTS, DPPH, and FRAP. The Korean landrace sorghums showed significant variations of metabolite contents and antioxidant activities by collection regions. Gyeonggi sorghum had the highest tannin and TPC contents with 273.73%, 7.395mgGAE/g, respectively. Therefore, three assays of antioxidant activities of Gyeonggi sorghum, which is highly related to tannin and TPC, were also significantly higher than others. However, Gyeongnam sorghums showed the lowest tannin and TPC with 148.34% and 3.482mgGAE/g, thus, three antioxidnat activities showed the lowest levels. Three accessions(IT322580, IT340261 and IT329053) had the highest tannin levels over 400%, but, two accessions (IT322613, IT270366) showed less than 1% of tannin content. Four accessions (IT270349, IT286448, IT331878, and IT251882) showed the highest level of TPC over 9mgGAE/g. In this study, antioxidant activities with three assays were strongly related to tannins and TPC, and TPC showed higher significance than tannin with all three antioxidant activities. The germplasms with the highest overall antioxidant activity were IT270349, IT340261, and IT286448, which had the highest levels of tannin and TPC. The principal component analysis presented that 2 sub-populations were divided. Gyeonggi and Gangwon Yeongseo sorghums, which had high metabolite contents and antioxidant activity levels, were mainly included in group 1. Gyeongnam and Jeonnam sorghums, which had low metabolite and antioxidant activity levels, were mainly included in group 2. This results could contribute to discover breeding metarials for the development of functional sorghum varieties.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.96-96
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• 2020

Soybean seeds are important sources of non-nutritive health promoting metabolites. The contents of these metabolites are affected by both genetic and environmental factors. In the present study, the contents of five common isoflavones including daidzin, genistin, glycitin, malonyldaidzin, and malonylgenistin were analyzed in 72 soybeans of different seed coat colors, diversity, and of different origins including China (22), Japan (9), USA (12), India (4), and Korea (25). The average total isoflavone content (TIC) was maximum in Indian soybeans (3302.36 ㎍/g) and minimum in Chinese landraces (1214.95 ㎍/g). The Korean landraces had higher average TIC (2148.05 ㎍/g) than the USA genetic materials (1580.23 ㎍/g) and Japanese landraces (1485.99 ㎍/g). The content of malonylgenistin was in the range of 54.31 - 2385.68 ㎍/g in the entire population, and was the most abundant isoflavone irrespective of origin although there was content variation among individual soybeans. Besides, glycitin was the least concentrated isoflavone, and its content ranged from 0.00 to 79.79 ㎍/g. With respect to seed coat color, green soybeans from all countries displayed the maximum malonylgenistin and TIC contents. Exceptions were those of Korean and Indian origins where black and yellowish-green soybeans presented the highest malonylgenistin and TIC contents, respectively. In multivariate analysis, 92.72% of the variance was explained by the first two principal components, and the soybeans were grouped in to three clusters based on isoflavone contents. Overall, our findings signify the importance of seed coat color and origin as discriminant parameters, and provide wide spectrum of routes for breeding soybean cultivars.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.640-641
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• 2003

Abnormal oceanic conditions associated with the passage of typhoons are examined using hydrographic and satellite data 1990-2002. During the passage period of typhoons in the Korean waters, an abrupt decrease of sea surface temperature(SST) in range of 5 to 8 $^{\circ}$ was observed. The areas of SST decrease were an order of 100-200km, and the low SST lasted about 15-25 days after passage of typhoon. After passage of typhoon, the water temperatures in the surface mixed layer of 30m show negative anomalies for quite a long period. In addition, stratification parameters were substantially decreased and chlorophyll a density was rapidly increased.

• Fisheries and Aquatic Sciences
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• v.1 no.1
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• pp.72-78
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• 1998

The larvae of Balanus improvisus were reared in the laboratory from hatching to cyprid. Larval development consists of six nauplius stages and a cyprid. Morphological features of all the nauplius stages are illustrated and described.