• Proceedings of the PSK Conference
• /
• 2001.04a
• /
• pp.153.1-153.1
• /
• 2001

• Microbiology and Biotechnology Letters
• /
• v.2 no.2
• /
• pp.111-117
• /
• 1974

Studies were carried out on the practical use of Naringinase and some chracteristics of Naringin solublizing enzme which might hydrolyae naringin to purunin. Obtained results were as follows. 1. Selected strain for Naringinase producing was identified to be Aspergillus niger S-1 and its naringinase was applied to chinese citron processing to remove the bitter taste. 2. Of the naringinase, naringin solubilizing enzyme was purified on a DEAE-Sephadex A-50 column and crystalized from acetone and ammonium sulfate. 3. Hydrolized naringin which has higher solubility rather than naringin or naringenin were identified by thin layer chromatography. 4. Hydrolyzed naringin and naringin were separatly determinated by ethylacetate extraction and this result was compared with sensory test.

• Journal of the Korean Wood Science and Technology
• /
• v.29 no.2
• /
• pp.133-139
• /
• 2001

The structures of six flavonoids isolated from heartwood of Prunus sargentii(Rosaceae) were analyzed by Mass and NMR spectrometry. These flavonoids were grouped into dihydroflavonol, flavanone, and flavanone glycoside, and identified as follows : 3,3',4',5,7-pentahydroxyflavanone(taxifolin) as a dihydroflavonol, 5-hydroxy-7-methoxyflavanone(pinostrobin), 4',5,7-trihydroxyflavanone(naringenin), 3',4',5,7-tetrahydroxyflavanone(eriodictyol), 5,7-dihydroxyflavanone(pinoccmbrin) as a flavanone and 7-hydroxyflavanone 5-O-${\beta}$-D-glucopyranoside(verecundin) as a flavanone glycoside.

• Culinary science and hospitality research
• /
• v.24 no.1
• /
• pp.63-72
• /
• 2018

The aim of this study was to investigate bioactive compounds from three domestic tomato varieties (Rafito, Momotaro TY Winner, and Medison). Lycopene, ${\beta}-carotene$ and polyphenols were quantified and identified using HPLC and LC-MS/MS. The levels of lycopene ranged from 28.36 mg/100 g to 60.18 mg/100 g. The content of ${\beta}-Carotene$ ranged from 2.00 mg/100 g to 2.92 mg/100 g. Ten kinds of polyphenol compounds were identified: caffeic acid-hexose isomer (I), caffeic acid-hexose isomer (II), 3-caffeoylquinic acid, 5-caffeoylquinic acid, caffeoylquinic acid isomer, quercetin-3-apiosylrutinoside, quercetin-3-rutinoside, di-caffeoylquinic acid, tri-caffeoylquinic acid, and naringenin chalcone. The level of 5-caffeoylquinic acid was the highest in domestic tomato varieties, ranging from 12.71 mg/100 g to 28.40 mg/100 g. The content of quercetin-3-rutinoside ranged from 3.74 mg/100 g to 17.64 mg/100 g. The contents of 3-caffeoylquinic acid and quercetin-3-apiosylrutinoside were 1.01~2.31 mg/100 g and 5.84~6.83 mg/100 g, respectively. Arrestingly naringenin chalcone was found only in Medison variety (36.82 mg/100 g). These results revealed that domestic tomato can be a good source of bioactive compounds for human health.

• BMB Reports
• /
• v.41 no.1
• /
• pp.68-71
• /
• 2008

Flavones are synthesized from flavanones through the action of flavone synthases (FNSs). There are two FNSs, FNS I and II. FNS I is a soluble dioxygenase present in members of the Apiaceae family and FNS II is a membrane bound cytochrome P450 enzyme that has been identified in numerous plant species. In this study, we cloned OsFNS I-1 from rice by RTPCR, expressed it in E. coli, and purified the recombinant protein. By NMR analysis, we found that OsFNS I-1 converted the flavanone (2S)-naringenin into the flavone, apigenin. Moreover, we found that the cofactors oxoglutarate, $FeSO_4$, ascorbate and catalase are required for this reaction. OsFNS I-1 encodes a flavone synthase I. This is the first type I FNS I found outside of the Apiaceae family.

• Korean Journal of Pharmacognosy
• /
• v.30 no.1
• /
• pp.34-39
• /
• 1999

To search for less toxic antiherpetic agents, the inhibitory effects of twelve kinds of flavonoids including chrysin, quercetin, quercitrin, rutin, fisetin, gossypin, kaempferol, morin, naringenin, naringin, hesperetin and hesperidin on the plaque formation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells were examined by plaque reduction assay in vitro. Some flavonoids tested in this study showed potent antiherpetic activity, reducing intracellular replication of herpes simplex viruses when Vero cell monolayers were infected and subsequently cultured in medium containing flavonoids. Naringenin showed the most potent antiviral activity against HSV-1 with selectivity index (SI) of 19.1 and hesperetin showed the most potent antiviral activity against HSV-2 with SI of 9.8. These results suggest that some flavonoids may be a potential therapeutic agent for the treatment and prevention of herpes simplex virus infections.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.5
• /
• pp.770-776
• /
• 2020

Genistein is a type of isoflavonoid found predominantly in leguminous plants. Genistein has diverse biological activities, such as anthelmintic and antioxidant effects, as well as inhibitory effects on the growth of several cancers. In addition, genistein is well known as a phytoestrogen. In this study, we attempted to biologically synthesize genistein from either p-coumaric acid or naringenin using Escherichia coli as a biotransformation host. Four genes, Os4CL, PeCHS, RcIFS, and OsCPR, were used for genistein production. To functionally express RcIFS and OsCPR, two members of the cytochrome P450 family, in E. coli, the membrane-binding anchor domain of each gene was removed, and RcIFS and OsCPR were translationally fused to generate an RcIFS-OsCPR hybrid. Os4CL and PeCHS, or the RcIFS-OsCPR hybrid, were then transformed into E. coli BL21(DE3). Using these strains, we optimized our culture system at a laboratory scale in terms of the cell density, concentrations of substrate and isopropyl-β-D-thiogalactoside, temperature, and culture medium. Under the optimized culture conditions, genistein was produced at up to 35 mg/l and 18.6 mg/l using naringenin and p-coumaric acid, respectively.

• Natural Product Sciences
• /
• v.17 no.3
• /
• pp.175-180
• /
• 2011

Eleven compounds were isolated from fresh leaves of Abies koreana (Pinaceae), and structures of these compounds were determined to be 3-hydroxy-2-methyl-4-pyrone (1), maltol-3-O-${\beta}$-D-glucoside (2), (-)-epicatechin (3), naringenin 7-O-${\beta}$-D-glucopyranoside (4), naringenin-7-O-rhamnoglucoside (5), kaempferol 3-O-${\beta}$-D-glucopyranoside (6), (+)-isolariciresinol (7), secoisolariciresinol (8), rhododendrol (9), ferulic acid (10) and 4-(4-hydroxyphenyl)butan-2-one (rheosmin) (11) by comparing $^1H$-, $^{13}C$-NMR and MS spectroscopic data with reference values. Compounds 3, 5, 7, 8, 9, 10, 11 were isolated for the first time from A. koreana. Among eleven isolates, compounds 1, 7 and 11 showed inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV-2 microglia in a concentration dependent manner.

• Korean Journal of Environmental Agriculture
• /
• v.27 no.4
• /
• pp.362-367
• /
• 2008

The legume-rhizobia symbiosis is an important source of plant growth and nitrogen fixation for many agricultural systems. This study was conducted to investigate the effects of salinity stress on nitrogen fixation and growth of pea (Pisum sativum L.), which has antimutagenic activities against chemical mutagen, inoculated with R. leguminosarum bv. viciae cultured with additional plant-to-rhizobia signal compounds, naringenin (NA,15 uM), methyl-jasmonate (MJ, 50 uM) or both, under greenhouse conditions. Three salinity levels (0.6, 3.0 and $6.0\;dS\;m^{-1}$) were imposed at 3 days after transplanting and maintained through daily irrigations. Addition of signal compounds under non-stress and stress conditions increased dry weight, nodule numbers, leaf area and leaf greenness. The inducers increased photosynthetic rate under non-stress and stress conditions, by approximately 5-20% when compared to that of the non-induced control treatment. Under stress conditions, proline content was less in plants treated with plant-to-bacteria signals than the control, but phenol content was significantly increased, compared to that of the control. The study suggested that pre-incubation of bacterial cells with plant-to-bacteria signals could enhance pea growth, photosynthesis, nitrogen fixation and biomass under salinity stress conditions.

• Archives of Pharmacal Research
• /
• v.13 no.2
• /
• pp.211-213
• /
• 1990

To test flavonoids for antibacterial activity against oral micraorganisms, flavonoids, quercetrin and naringenin, were incorporated into two pharmaceutical preparations in the form of tooth paste. Samplees of dental plaque, the msot accused dental deposit which initiates the gingival and periodental diseases, were collected from the teeth surface of ten dental students at one week interval before and after using placebo, followed by two formulae of tooth paste containing 0.1% of quercetrin and naringenin (formulas I and II, respectively). The amount of dental plaque was assessed by the quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The amount of dental plaque was assessed by the Quigley and Hens index. Then plaque samples were subjected to bacteriological examination of Gram stain and plate counts of microorganisms. The results revealed that most of Gram negative cocci and bacilli were highly affected by the two formulae : the number of actinomycetes were decreased after using formula I and disappeared completely by the sue of formula II, while the number of Gram positive streptococci was highly decreased after the treatment with the two formulae. These results indicate a possible use of flavonoids to inhibit dental plaque formation.