• 제목/요약/키워드: Nano-bio technology

검색결과 533건 처리시간 0.026초

Glass Slide-based Immunosensing for C-Reactive Protein Using Quantum Dot-Antibody Conjugate

  • Kim, Namsoo;Oh, Sun Mi;Kim, Chong-Tai;Cho, Yong Jin
    • 산업식품공학
    • /
    • 제14권1호
    • /
    • pp.21-26
    • /
    • 2010
  • C-Reactive protein (CRP), which is an 118 kDa pentameric protein, was secreted by the liver is an important biomarker for coronary disease, hypertension and inflammation. In this study, a method for CRP detection exploiting quantum dot (Qdot)-antibody conjugate was developed according to an indirect-competitive immunosensing protocol. For this purpose, a streptavidin-bound $Qdot_{605}$ was linked with a separately prepared biotinylated monoclonal antirat CRP antibody to produce a Qdot-antibody conjugate. The immunosensing was performed at 0.1 and 20 nM of the coating antigen and conjugate, respectively. The current method was found very sensitive in CRP detection, judging from the concentration-dependent fluorescence emission.