Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
권호 표지

Glass Slide-based Immunosensing for C-Reactive Protein Using Quantum Dot-Antibody Conjugate

  • Kim, Namsoo (Food Bio-nano Technology Research Group, Korea Food Research Institute) ;
  • Oh, Sun Mi (Food Bio-nano Technology Research Group, Korea Food Research Institute) ;
  • Kim, Chong-Tai (Food Bio-nano Technology Research Group, Korea Food Research Institute) ;
  • Cho, Yong Jin (Food Bio-nano Technology Research Group, Korea Food Research Institute)
  • Received : 2009.10.07
  • Accepted : 2010.02.04
  • Published : 2010.02.28
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Abstract

C-Reactive protein (CRP), which is an 118 kDa pentameric protein, was secreted by the liver is an important biomarker for coronary disease, hypertension and inflammation. In this study, a method for CRP detection exploiting quantum dot (Qdot)-antibody conjugate was developed according to an indirect-competitive immunosensing protocol. For this purpose, a streptavidin-bound $Qdot_{605}$ was linked with a separately prepared biotinylated monoclonal antirat CRP antibody to produce a Qdot-antibody conjugate. The immunosensing was performed at 0.1 and 20 nM of the coating antigen and conjugate, respectively. The current method was found very sensitive in CRP detection, judging from the concentration-dependent fluorescence emission.

Keywords

Acknowledgement

Supported by : Korea Food Research Institute

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