• 한국염색가공학회지
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• 제29권4호
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• pp.256-267
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• 2017

The purpose of this study was to analyze the functional ingredients of Perilla Frutescens L. Britt extracts and to confirm the possibility of producing PVA nanofibers using extracts. Distilled water, 3% aqueous sodium hydroxide solution and ethanol were used as extraction solvents. The electrospinning was carried out at a PVA concentration of 12%, an applied voltage of 10 kV and a tip to collector distance of 15cm. The contents of volatile substances, essential oils, total polyphenols and flavonoids of the extracts were measured to examine the constituents of functional materials. Flavor components and esters were identified in 3% sodium hydroxide and ethanol extracts. The content of polyphenols and flavonoids in ethanol extracts was higher than that of medicinal plants. 1wt.% of Tween 20 was added to disperse the essential oil components of the ethanol extract. Addition of a dispersant made it possible to produce a homogeneous mixture by having some compatibility with the ethanol extracts and the PVA molecule. When the concentration of the ethanol extract was 0.25 and 0.5wt%, relatively uniform PVA nanofiber having an average diameter of 350 to 365nm could be produced. The results of FT-IR, XRD and DSC analysis confirmed that Perilla Frutescens L. Britt ethanol extract was well mixed with PVA molecules and was electrospun.

• 한국토양환경학회지
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• 제5권1호
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• pp.45-54
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• 2000

본 연구는 중금속(Zn, Cd)으로 오염된 토양을 여러 세척 용제(물, mineral acid, chelating agent, organic acld)를 가지고 탈착시켜 제거하는 실험으로 pH와 농도에 따라 회분식과 연속식 실험을 병행하였다. 물과 NaOH에 의한 세척 효과는 거의 없는 것으로 나타났으며 HCI, EDTA, Oxalic acid에 의한 세척 효과는 매우 높게 나타났다. HCI에 의한 세척효과가 가장 높은 이유는 낮은 pH에 의한 중금속의 이온화 현상에 의한 것으로 사료되며 EDTA, Oxalic acid의 효과가 높은 것은 용해도를 증가시켜 중금속 이온과 많은 착물을 형성하기 때문이다. 또한 Zn이 Cd보다 대체적으로 탈착율이 좋았으며 일반적으로 제거 경향은 pH가 낮을수록, 세척 용제의 농도가 높을수록 탈착율이 증가하였다. 중금속으로 오염된 토양 복원시 Zn의 세척 용제로는 HCI 과 EDTA를, Cd의 세척 용제로는 HCI과 Oxalic acid를 가지고 세척을 함으로써 효과적으로 정화되는 것을 알 수 있었다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.46-46
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• 2003

Salviae Miltiorrhizae Radix has been used for treatment of cardiovascular diseases in oriental medicine. To investigate the possible involvement of cardiac ion channel in this effect, we examined electrophysiological effects of the extract of Salviae Miltiorrhizae Radix on action potentials and ionic currents in rat ventricular myocytes. The extracts of Salviae Miltiorrhizae Radix were fractionated into nine fractions, and the effect of each fraction on action potential was tested. The fraction containing monomethyl lithospermic acid-A (LSA-A) induced a significant prolongation of action potential duration (APD). LSA-B which is a major component of Salviae Miltiorrhizae Radix, however, did not cause a significant effect. In voltage clamp experiments, the effects of LSA-A on K currents, Ca currents and Na currents were tested. Neither K currents nor L-type Ca currents were affected by LSA-A. On the contrary, LSA-A significantly slowed down the inactivation kinetics of the Na current with no effect on the fast component of the inactivation process. The amplitude of the peak current and the voltage-dependence of activation were not changed by LSA-A. The effect of LSA-A on Na current was abolished when high concentration of $Ca^{2+}$ buffer (10 mM BAPTA) was included in the pipette solution or when Ca2+ current was blocked by nicardipine (1 $\mu$M) in the bath solution.n.

• 한국식품영양학회지
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• 제10권3호
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• pp.375-381
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• 1997

식용버섯을 대상으로 항응고 활성을 검색한 결과 영지버섯 알칼리 추출물이 가장 높은 활성을 보였다. 영지버섯으로부터 추출한 조다당 획분인 GL-I은 1N NaOH로 8시간 추출물(GL-0)을 methanol 환류, 에탄올 침전을 거쳐 투석, 동결건조하여 조제하였다. 히-I 획분은 periodate 산화에 의해서는 활성이 크게 변하지만 pronase 소화시 활성의 변화가 거의 없는 것으로 보아 항응고 활성의 본체는 주로 당과 관계되는 것으로 추정된다. 히-I의 구성당은 glucose:galactose:xylose:mannose:arabinose가 19.3:3.0:2.3:1.3:1.0:0.3의 molar ratio로 구성되어 있다. GL-I 획분을 DEAE-Toyopearl 650C(GL-Ia$\longrightarrow$If)와 Sephadex G-100(GL-Ic-I$\longrightarrow$GL-Ic-II)을 이용하여 부분 정제하였다.

• 자원리싸이클링
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• 제25권3호
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• pp.63-73
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• 2016

본 연구에서는 해수로부터 마그네슘을 농축하기 위하여 세 가지 침전제(NaOH, 시멘트 킬른 더스트, 제지슬러지소각재)를 이용하여 마그네슘을 침전시키고, 황산을 이용하여 마그네슘을 용출하는 실험을 진행하였다. 사용한 침전제의 종류와 상관없이 침전반응 효율은 거의 100%이었고, 용출반응 효율은 77 - 89%로 비슷하였다. 용출액의 마그네슘 농도가 4975 - 5775 mg/L이었는데, 이것은 침전반응과 용출반응을 통해 해수의 마그네슘이 3.8 - 4.4배 농축되었음을 의미한다. 본 연구에서 침전제로 사용한 산업부산물의 반응효율은 거의 100%로 기존의 고가 침전제와 비슷하였다.

• 공업화학
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• 제5권1호
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• pp.90-98
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• 1994

구연산-초산-유산계에서 구연산의 반응추출에 영향을 미치는 여러 인자를 규명하기 위하여 추출제로 2급과 3급아민 및 용매화추출제를, 용매로 n-butylacetate, MIBK, kerosine 및 xylene 등을, modifier로서 tributyl-phosphate(TBP)와 isodecanol이 이용되었다. 그 외에 수용액상의 pH와, 온도의 영향을 연구하였고 평형상수도 얻었다. NaOH, $Na_2CO_3$ 및 $K_2HPO_4$ 알칼리 수용액에 의한 재추출 실험도 하였다. 실험결과 구연산의 추출에서 2급아민인 di-isotridecylamine(DITDA)이 추출도와 선택성에서 가장 좋았고 용매로서는 n-butylacetate가, modifier로서는 TBP가 우수하였다. 이외에도 수용액의 pH와 추출계의 온도가 낮을수록 추출도가 높았다. 재추출도는 stripping상의 염기도와 온도가 낮을수록 높아졌고 알카리 농도가 커질수록 높아졌다.

• 한국식품저장유통학회지
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• 제24권4호
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• pp.529-535
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• 2017

Rapeseed cake was extracted with 80% ethanol and then fractionated with $H_2O$ (fraction I) as well as with 30% (II), 50% (III), 70% (IV), and 100% ethanol (V). Total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric-reducing antioxidant potential, and Trolox equivalent antioxidant capacity were in the order of fractions II > III > I > IV > V. The three fractions with high antioxidant activities and TPC (I, II, and III) were pooled and hydrolyzed by NaOH solution, resulting in 18.97 mg sinapic acid/g hydrolyzed extract and 21- and 2.2-fold increases in TPC and DPPH radical scavenging activity, respectively. Hydrolyzed rapeseed cake extracts (200, 500, and 1,000 ppm) and catechin (200 ppm) as a comparison were added to 10% fish oil-in-water emulsion, and their effects on oxidative stability were investigated by measuring hydroperoxide values (PV) during refrigerated storage. PVs were significantly lower in the emulsions with added hydrolyzed extract as compared to the control (p<0.05) and significantly decreased with increasing extract concentration (p<0.05) over a period of 29 days. The emulsion added with hydrolyzed extract showed higher PV than that added catechin at the same concentration (200 ppm) during 13-22 days (p<0.05), but after then, the PV was not significantly different (p>0.05). This study indicates that hydrolyzed rapeseed cake extract rich in sinapic acid may inhibit oxidation in a fish oil-in-water emulsion in a concentration-dependent manner.

• Biomolecules & Therapeutics
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• 제9권3호
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• pp.162-166
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• 2001

Helicobacter pylori is a major cause of gastric-associated diseases. In our previous study, the Adhesin/CTXA2B was expressed as insoluble recombinant chimeric protein derived from the H. pylori adhesin genetically coupled to CTXA2B subunit in Escherichia coli. Since it is very important to optimize IPTG concentration, culture temperature and composition of medium to maximize cell growth and productivity, these conditional growth factors were determined for increasing the productivity of the expressed Adhesin/CTXA2B chimeric protein in Escherichia coli JM101 carrying pTEDhpa/ctxa2b. Our data demonstrate that optimal medium for increased production of chimeric protein was a YCP/Glu medium composed of 2% yeast extract, 1% casamino acid, phosphate solution [0.3% $KH_2P0_4$, 0.4% $Na_2HP0_4$, 0.25% ($NH_4)_2HPO_4$], and 0.5% glucose. In addition, optimal concentration of IPTG was 1 mM and culture temperature, $37^{\circ}C$.

• 한국식품영양과학회지
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• 제29권5호
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• pp.783-789
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• 2000

This study was focused on the optimal extracting conditions of dimethyl-$\beta$-propiothetin as bioactive substance from green seaweed. Identification and quantification of dimethyl-$\beta$-propiothetin were measured by headspace gas chromatography after conversion to dimethyl sulfide by treatment with saturated NaOH solution. Dimethyl-$\beta$-propiothetin was extracted through various processes (solvent extraction, ultrasonication, boiling and autoclaving) from Ulva pertusa. The content of dimethyl-$\beta$-propiothetin extracted by autoclaving treatment showed higher than that of various extraction methods. Dimethyl-$\beta$-propiothetin content in extract of Ulva pertusa was 325,800 ng/g after autoclaving 121$^{\circ}C$ for 45 min. Dimethyl-$\beta$-propiothetin in exract of Ulva pertusa was comparative stable under low temperature. The retentions of dimethyl-$\beta$-propiothetin content in extract of Ulva pertusa were 76.6~99.8% by incubation at 10~6$0^{\circ}C$ for 2 hours. Chemical decomposition of dimethyl-$\beta$-propiothetin was observed under laboratory conditions at pH values higher than 9.5.

• 패션비즈니스
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• 제14권2호
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• pp.138-150
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• 2010

In the dyeing of cotton and silk fibres using Caesalpinia sappan, the effects of pH changes of the Caesalpinia sappan dye liquor on the color were examined. In order to adjust the pH of the Caesalpinia sappan dye liquor, Schisandra chinensis extract and carboxylic acid were used for acidic condition, lye and the NaOH aqueous solution were used for alkaline condition. By introducing the dyeing method of pH adjustment, firstly, the effect on the reddish color inherent to the Caesalpinia sappan was examined. At the same time, the manifestation of the yellow color, which affect the manifestation of the red color greatly, was examined in detail quantitatively. By dyeing the cotton and silk fabrics, the inherent relationships established between the pH hanges and the fibre characteristics were sought to be examined.