• 한국문화재보존과학회:학술대회논문집
• /
• 2005.11a
• /
• pp.122-134
• /
• 2005

XRF, EDX, IR analysis was conducted to figure the effect of Fe to blacken the waterlogged wood. The results showed that investigated soil contained more Fe than normal soil by XRF analysis and wood ash contained more sulfur and Fe than any other element by EDX analysis. C-H and C-O peaks were significantly reduced at the surface of wood where is blackened part of waterlogged wood by IR analysis. The optimum condition to remove Fe from waterlogged wood by EDTA was investigated. To do this, removed concentration of Fe was measured at various concentration of EDTA-2Na. The optimum pH of EDTA-2Na was figured to be 4.1 to 4.3 and as the concentration of EDTA was increasing, extracted concentration of Fe was also increased. In the case of 0.4 wt% of EDTA-2Na, 700ppm of Fe was eliminated and was stabilized after 48 hours time lapse. In the case of EDTA-3Na, the optimum pH was 7 to 8, and 10 ppm of Fe was eliminated at 0.4 wt% of EDTA-3Na. In the case of EDTA-4Na, the optimum pH was 10 to 11, and 120 ppm of Fe was eliminated at 0.4 wt% of EDTA-4Na.

• Korean Journal of Heritage: History & Science
• /
• v.40
• /
• pp.413-430
• /
• 2007

This study analyzed the foreign substances in waterlogged archaeological woods and compounds in soil where waterlogged archaeological wood was buried, in order to examine the relationship between burial environment and foreign substances in waterlogged archaeological wood. The XRF(X-ray Fluorescence Spectroscopy) and EDX(Energy Dispersive X-ray) analysis were conducted to examine the effect of iron(Fe) to blacken the waterlogged wood. The XRF results showed that investigated soil contained Si, Al, and Fe. Wood ash contained more sulfur and Fe than any other elements in the EDX analysis. Cellulose and hemicellulose were significantly reduced at the surface of wood, which is the blackened part of waterlogged wood. Foreign substances changed the surface color. These problems could be solved by removal of foreign substances in waterlogged archaeological wood using EDTA(Ethylene Diamine Tetra Acetic acid). The optimum condition to remove Fe from waterlogged wood by EDTA was investigated. To do this, the concentration of Fe removed was measured with various concentration of EDTA-2Na. The optimum pH of EDTA-2Na was figured to be 4.1 to 4.3. As the concentration of EDTA increased, the extracted concentration of Fe also increased. In the case of 0.4 wt% of EDTA-2Na, about 60ppm of Fe was eliminated and was stabilized after 48 hours. In the case of EDTA-3Na, the optimum pH was 7 to 8, and about 10 ppm of Fe was eliminated at 0.4 wt% of EDTA-3Na. In the case of EDTA-4Na, the optimum pH was 10 to 11, and about 20 ppm of Fe was eliminated at 0.4 wt% of EDTA-4Na. In conclusion, the iron(Fe) in waterlogged archaeological wood was removed by EDTA treatment and it increased the whiteness of the surface.

• Journal of Plant Biotechnology
• /
• v.50
• /
• pp.183-189
• /
• 2023

This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. 'Shiny Pink' and 'Pink Holic' exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, 'Snow Dream,' 'Harmony,' and 'Breeze' remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in 'Breeze,' 'Harmony,' and 'Snow Dream' than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in 'Pink Holic,' particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.

• Journal of the Korean Chemical Society
• /
• v.12 no.3
• /
• pp.114-120
• /
• 1968

Fe-EDTA complex, which is easily formed when Fe salt and EDTA.2Na are mixed in the aqueous medium, is found to be a very effective catalyst in the dehydropolycondensation of aminophenols. In the dehydropolycondensation of aminophenols, the catalyst, Fe(Ⅲ)-EDTA complex (higher oxidation state) is reduced to less stable Fe(Ⅱ)-EDTA complex (lower oxidation state), and the latter is easily oxidized by air to the original higher oxidation state complex, therefore the catalytic action of Fe-EDTA complex is found to be recycled effectively. Under the catalytic action of the above mentioned complex, p-aminophenol is polymerized in the aqueous medium to form the oligomers of p-aminophenol, which the degree of polymerization to be 5 or more. The oligomers formed contain partly quinone nucleus as well as amino and hydroxyl groups. In this study, the effects of the solvents and characteristics of the oligomers are discussed. These types of polymerizations catalyzed by the metallic chelate compounds are considered to be very closely related to the reactions in the living matters.

• Korean Journal of Community Nutrition
• /
• v.11 no.6
• /
• pp.771-778
• /
• 2006

Previous studies have suggested that delta-aminolevulinic acid dehydratase (ALAD) phenotype differently affect mineral metabolism. The objective of this study was to determine the effectiveness of 6-month iron supplementation as syrup of NaFeEDTA in improvement of iron status according to ALAD genotype. One hundred thirty adult women living in rural areas of Asan were provided NaFeEDTA syrup once a week for 6 months at the dose of 64mg Fe/week. Three hundred control subjects were observed during the study period. Fasting blood was obtained for analyzing hemoglobin (Hb) and zinc protophorphyrin (ZPP) and serum was analyzed for ferritin, iron and total iron capacity (TIBC) levels before and after iron supplementation. Ninety percent of ALAD 1-1 (ALAD1) and 10% of ALAD 1-2 (ALAD2) genotype were observed in the control group. However in the intervention group, 98% showed ALAD1 while only 2% was ALAD2, which is significantly lower proportions of ALAD2 compared to the control group (p<0.01). The iron status of Intervention group significantly improved except for ferritin and TIBC regardless or ALAD genotype, while the control group did not show any changes in iron status except for ZPP. ZPP concentration of the control group significantly increased in both ALAD1 and 2 while the intervention group showed significantly decreased ZPP after supplementation in ALAD1. Iron supplementation in the form of NaFeEDTA seems to be effective in reduction of ZPP levels although ALAD2 did not show significant changes due to the small number. However, it is difficult to make a conclusion from these results, and more specified further investigation is needed with more participants.

• Applied Biological Chemistry
• /
• v.14 no.3
• /
• pp.177-182
• /
• 1971

A new method for the measure of iron pools using 0.02M EDTA and $Na_2S_2O_4$ was tested on Akagare diseased and healthy rice leaf tissue 1) The proposed method could fraction iron into four fractions; ferrous iron($Fe^{++}$), ferric iron($Fe^{+++}$) precipitated iron(PFe) and bound iron(BFe) well indicating the physiological status of tissue. 2) The pattern of iron pools appears to be $Fe^{+++}>PFe>BFe>Fe^{++}$ in most physiologically favorable status of iron, $PFe>Fe^{+++}>BFe>Fe^{++}$ in favorable status, $BFe>Fe^{+++}>PFe>Fe^{++}$ in unfavorable status and $BFe>PFe>Fe^{+++}>Fe^{++}$ in toxic status. 3) The percentage of each fraction to total iron was less than 10 for $Fe^{++}$, 20 to 40 for $Fe^{+++}$ and PFe and 20 to 50 for BFe. 4) Ferrous iron was always higher in upper half leaf, the appearance of which is less healthier than lower half indicating that there is more active metabolic system in which ferrous iron is involved.

• Journal of Soil and Groundwater Environment
• /
• v.13 no.2
• /
• pp.44-53
• /
• 2008

The efficiences of mineral acid (HCl), neutral salts ($CaCl_2$), and chelating agent (citric acid and $Na_2$-EDTA) were tested for extracting heavy metals from open burning and open detonation (OBOD) site soil. The extraction efficiencies of Cd, Cu, Pb and Zn from soil for various extractants were in the order of HCl > citric acid > $Na_2$-EDTA > $CaCl_2$, HCl (1.0 M) extracted effectively 82%, 86%, 80%, and 46% of initial total concentrations of Cd, Cu, Pb, and Zn, respectively. Significant negative correlations were observed between pH of extractant and amount of extracted heavy metals. Initially, examined heavy metals were predominantly bound to carbonate and Fe, Mn-oxide fraction. Though the significant amount of carbonate and Fe, Mn-oxide bounded metals were removed but a significant amount remained metals shifted to exchangeable (more mobile) fraction by HCl and citric acid extraction. The increased mobility of remaining metals could be problematic for water resources, thus careful management is needed to control the movement of heavy metals.

• Journal of Plant Biology
• /
• v.29 no.3
• /
• pp.197-205
• /
• 1986

Leaf mesophyll protoplasts from five cultivars of tobacco (N. tabacum L.) were cultured. The protoplasts did not survive in culture medium containing Murashige and Skoog inorganic salts for over 6 days. NH4NO3 and FeSO4.Na2EDTA concentration of Murashige and Skoog medium were toxic in tobacco leaf mesophyll protoplast culture. Therefore we investigated optimum condition in Murashige and Skoog medium. High plating efficiency was obtained by reducing the concentrations of NH4NO3 and FeSO4.Na2EDTA to 1/3 and 1/10, respectively, on the supplemented with 5$\mu$M IAA, 0.5 $\mu$M 2.4-D 5 $\mu$M BAP. Plants were regenerated from protoplast-derived calluses.

• Journal of Life Science
• /
• v.8 no.5
• /
• pp.598-603
• /
• 1998

A marine microbe, Vibrio anguillarum was isolated from fish and studied for its concerning pathogenic substance of hemolysin. Purification of hemolysin was achieved by the procedure of ammonium sulfate precipitation from cul-ture filtrate, DEAE-cellulose chromatography, and G-200 gel filtration with 36 fold of purification and 2.3% yield. The molecular weight of the purified hemolysin was 38,000 dalton by SDS-PAGE. The purified hemolysin was stable at pH 6-9, below 45$^{\circ}C$, and up to 1% of NaCl, respectively. $Ca^{2+}, Cu^{2+}, Zn^{2+}, Fe^{2+}$ inhibited the hemolytic activity whereas EDTA and $Mg^{2+}$ did not.

• Journal of the Korean Society of Safety
• /
• v.14 no.1
• /
• pp.86-92
• /
• 1999

This study was carried out to investigate the effect of chemical cleaning of corrosion product on cooling system made of copper as a basic material and using cooling water as pure water. We studied chemical cleaning condition that minimizes the influence on basic material by means of EDTA solution so as to eliminate the slurry in cooling system. In addition, the proper amount of NALCO-39L (Nitrite-Borate-BZT mixture) as a inhibitor was determined in order to protect the copper in cooling system against corrosion after chemical cleaning and the effect of corrosion resistance on the copper surface treated was excelent in comparison with surface untreated. As a result, we found that the main components of sludge in cooling system produced by corrosion of copper were $Cu_2O$, CuO, Cu, and Fe. The optimum condition of chemical cleaning was 400ppm EDTA solution at $60^{\circ}C$. Inhibitor concentration needed to treat the surface of pure copper was 15～20ppm per unit area and corrosion rate of copper treated with 500ppm inhibitor solution for 72 hrs at $60^{\circ}C$ was remarkably decreased as compared with that of pure copper.