• 제목/요약/키워드: NaCl concentration

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부영양화 해역에서 분리된 Vibrio sp. S-1-2균의 특성과 Cd축적에 관한 연구 (Studies on Bacterial Characteristics and Cd Accumulation of Vibrio sp. S-1-2, Isolated from Eutrophic Coastal Area)

  • 이원재;윤덕현;김무찬;박영태
    • 한국어병학회지
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    • 제4권1호
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    • pp.31-39
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    • 1991
  • 부영양화된 해역인 마산만에서 분리된 해양세균중 카드뮴 200ppm에서 내성을 보인 균을 분리배양하고, 미생물학적 성질과 생체 축적량을 방사성 동위원소인 $^{109}CdC_2$을 이용하여 측정한 결과는 다음과 같다. 1. 분리된 카드뮴 내성균은 Gram음성균이며 단간균으로서 운동성이 있고 생리, 생화학 특성과 TCBS 배지에서의 집락 형태로 보아 Vibrio sp.으로밝혀졌다. 2. 본 균의 다른 중금속에 대한 내성 실험에서 $ZnCl_2$, $PbCl_2$, $CuCl_2$가 25ppm 함유된 배지에서는 서로 비슷한 성장을 보였지만 $HgCl_2$ 25ppm에서는 성장이 거의 안되었다. 3. 균체내의 카드뮴 축적량은 배지에 포함된 카드뮴의 양과 비례하여 증가하여 75ppm에서 43.2mg/l로 최고의 축적을 나타내었고, 100ppm에서의 성장이 저해되었지만 균체 축적량이 일정하게 유지되었다. 4. 방사성 동위원소인 $ ^{109}CdCl_2$를 이용하여 25ppm에서 배양한 후, 중금속 축적조건을 dpm값을 통해 조사한 결과는 96시간 배양시 pH 7에 최대값인 $2554{\times}10^4dpm/m{\ell}$을 나타냈고, NaCl에서는 36시간 배양시 $611{\times}10^4dpm/m{\ell}$로 최고값을 나타내었다.

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불용성 전극의 Dye 제거 성능과 산화제 생성 비교 (Comparison of Dye Removal Performance and Oxidants Formation of Insoluble Electrode)

  • 유영억;김동석
    • 한국환경과학회지
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    • 제20권10호
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    • pp.1273-1284
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    • 2011
  • The aim of this research was to evaluate the performance of insoluble electrode for the purpose of degradation of Rhodamine B (RhB) and oxidants generation [N,N-Dimethyl-4-nitrosoaniline (RNO, indicator of OH radical), $O_3$, $H_2O_2$, free Cl, $ClO_2$)]. Methods: Four kinds of electrodes were used for comparison: DSA (dimensional stable anode; Pt and JP202 electrode), Pb and boron doping diamond (BDD) electrode. The effect of applied current (0.5~2.5 A), electrolyte type (NaCl, KCl and $Na_2SO_4$) and electrolyte concentration (0.5~3.5 g/L) on the RNO degradation were evaluated. Experimental results showed that the order of RhB removal efficiency lie in: JP202 > Pb > BDD ${\fallingdotseq}$ > Pt. However, when concerned the electric power on maintaining current of 1 A during electrolysis reaction, the order of RhB removal efficiency was changed: JP202 > Pt ${\fallingdotseq}$ Pb > BDD. The total generated oxidants ($H_2O_2$, $O_3$, free Cl, $ClO_2$) concentration of 4 electrodes was Pt (6.04 mg/W) > JP202 (4.81 mg/W) > Pb (3.61 mg/W) > BDD (1.54 mg/W), respectively. JP202 electrode was the best electrode among 4 electrodes from the point of view of performance and energy consumption. Regardless of the type of electrode, RNO removal of NaCl and KCl (chlorine type electrolyte) were higher than that of the $Na_2SO_4$ (sulfuric type electrolyte) RNO removal. Except BDD electrode, RhB degradation and creation tendency of oxidants such as $H_2O_2$, $O_3$, free Cl and $ClO_2$, found that do not match. RNO degradation tendency were considered a simple way to decide the method which is simple it will be able to determinate the electrode where the organic matter decomposition performance is superior. As the added NaCl concentration was increases, the of hydrogen peroxide and ozone concentration increases, and this was thought to increase the quantity of OH radical.

3차원 전극을 사용한 Rhodamine B의 전기분해에 미치는 운전인자의 영향 (Effect of Operating Parameters on Electrochemical Degradation of Rhodamine B by Three-dimensional Electrode)

  • 김동석;박영식
    • 한국환경보건학회지
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    • 제35권4호
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    • pp.295-303
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    • 2009
  • A simulated wastewater containing the dye Rhodamine B (RhB) was electrolytically treated using a three-dimensional electrode reactor equipped with granular activated carbon (GAC) as particle electrode. The effect of type of packing material (GAC, ACF, Nonwoven fabric fiber coated with activated carbon), amounts of GAC packing (25-100 g), current (0.5-3 A) and electrolyte concentration (0.5-3 g/l) was evaluated. Experimental results showed that performance for RhB decolorization of the 3 three-dimensional electrodes lie in: GAC > Nonwoven fabric fiber > ACF. When considered RhB decolorization, oxidants concentration and electric power, optimum GAC dosage was 50 g. Generated concentration of 3 oxidants ($ClO_2$, free Cl, $H_2O_2$) was increased with increase of applied current, however optimum current for RhB degradation was 2.5 A. The oxidants concentration was increased with increase of NaCl concentration and optimum NaCl dosage for RhB degradation was 1.5 g/l.

5령유충의 배맥관운동에 대한 양이온의 영향에 관한 연구 (Studies on the Effect of Cation on the Activity at the 5th Instar Larvae of Bombyx mori)

  • 윤종관;사기언
    • 한국잠사곤충학회지
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    • 제18권2호
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    • pp.89-93
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    • 1976
  • 동물에 대한 생리적 염류용액은 소위 링가씨액으로서 냉혈척추동물의 신경근육 심장등을 비교적 정상에 가까운 상태로 지속시키기 위한 용액으로 개구리 (Frog)의 심장의 관수실험을 하기 위하여 처방한 최초의 생리적 염류용액에 지나지 않았지만 현실에 있어서는 몇몇 동물은 물론 사람에 이르기까지 거의 완전한 생리적 용액으로 되어 있는데 가잠에 있어서는 몇가지 처방이 발표된 바는 있어도 아직 그 결과가 만족할 만한 정도에까지는 이르지 못하고 있다. 일반적으로 생리적 염류용액은 전술한 바와 같이 적출한 기관과 조직에 대하여 장시간에 걸친 정상적 기능을 유지시키기 위한 용액으로 사용되는 염류의 혼합용액이기 때문에 적당한 Ion조성 삼투압 PH를 갖도록 조제된다. 대개 생기내에서 이들의 기관과 조직을 적시는 혈청등 체액성분에 근사한 것을 사용한다. 용액의 Ion성분은 $Na^{+}$을 비롯하여 $K^{+}$, $Ca^{++}$, $Mg^{++}$ 등을 가하고 여기에 NaHCO$_3$, NaH$_2$PO$_4$등의 연형제를 첨가하므로서 PH를 조절한다. 생리적 염류용액중에 포함하여야 할 Cation의 최적농도 2종 이상의 Cation의 최적혼합비율등에 대하여 가잠유충의 배맥관 운동을 지표로 하여 이를 검토하였다. 각 Cation의 최적농도는 영점미만의 NaCl의 함유액을 기본으로 하고 여기에 각각 농도를 달리한 KCl, CaCl$_2$및 MgCl$_2$등을 조합하여 이들의 액체를 적하하므로서 5령유충의 배맥관 운동의 지속시간과 맥박수를 지표로 하여 이들 시험액의 효과를 검토한 바에 의하면 NaCl 단독일 경우에는 해부후 일시적으로 맥박수는 증가하지만 점차 정지되고 만다(일시간정도). 여기에 대하여 KCI를 가하면 일반적으로 박동시간이 연장되고 맥박수의 저하도 늦어지는 경향을 나타내고 있으며 여기에 다시 KCl와 CaCl$_2$를 가하면 그 지속시간은 더 한층 연장되었다. KCI와 CaC1$_2$의 가용은 효과가 있지만 이들의 농도와 배맥관의 활동에 있어서는 일정한 경향이 인정되지 않았고 $Ca^{++}$ 또는 $K^{+}$의 박동에 대한 작용은 이것을 분명히 할 수 없었고 $Mg^{++}$의 작용에 있어서는 배맥관에 이를 가하면 박동 지속시간에는 영향을 끼치지 않지만 맥박수는 크게 저하됨을 인정할 수 있었다.

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Growth Characteristics of Enterobacter sakazakii Used to Develop a Predictive Model

  • Seo, Kyo-Young;Heo, Sun-Kyung;Bae, Dong-Ho;Oh, Deog-Hwan;Ha, Sang-Do
    • Food Science and Biotechnology
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    • 제17권3호
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    • pp.642-650
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    • 2008
  • A mathematical model was developed for predicting the growth rate of Enterobacter sakazakii in tryptic soy broth medium as a function of the combined effects of temperature (5, 10, 20, 30, and $40^{\circ}C$), pH (4, 5, 6, 7, 8, 9, and 10), and the NaCl concentration (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10%). With all experimental variables, the primary models showed a good fit ($R^2=0.8965$ to 0.9994) to a modified Gompertz equation to obtain growth rates. The secondary model was 'In specific growth $rate=-0.38116+(0.01281^*Temp)+(0.07993^*pH)+(0.00618^*NaCl)+(-0.00018^*Temp^2)+(-0.00551^*pH^2)+(-0.00093^*NaCl^2)+(0.00013^*Temp*pH)+(-0.00038^*Temp*NaCl)+(-0.00023^*pH^*NaCl)$'. This model is thought to be appropriate for predicting growth rates on the basis of a correlation coefficient (r) 0.9579, a coefficient of determination ($R^2$) 0.91, a mean square error 0.026, a bias factor 1.03, and an accuracy factor 1.13. Our secondary model provided reliable predictions of growth rates for E. sakazakii in broth with the combined effects of temperature, NaCl concentration, and pH.

n-부탄올 및 NaCl 수용액에서 양이온 계면활성제인 TTAB에 의한 크레졸 이성질체들의 가용화에 대한 연구 (Solubilization of Cresol Isomers by the Cationic Surfactant of TTTAB in Aqueous Solution of n-Butanol and NaCl)

  • 이병환
    • 한국응용과학기술학회지
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    • 제38권3호
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    • pp.832-839
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    • 2021
  • 양이온 계면활성제인 tetradecyltrimethylammonium bromide (TTAB)에 의한 크레졸 이성질체의 가용화에 미치는 치환기, 온도 그리고 NaCl과 n-부탄올과 같은 첨가제의 효과를 UV-Vis법으로 측정하였다. 가용화상수(Ks)값은 o-크레졸s값은 감소하였다. 크레졸 이성질체들의 가용화에 대하여 계산한 ∆Go값과 ∆Ho값은 모두 음의 값을 나타내었다. 그러나 ∆So값은 모두 양의 값을 나타내었다. 또한 ∆Go값은 n-부탄올의 농도가 증가할수록 증가하는 경향을 그러나 NaCl의 농도가 증가할수록 더욱 감소하는 경향을 보였다. 이러한 사실들로부터 크레졸 이성질체들의 가용화에는 엔탈피와 엔트로피가 동시에 기여하고 있으며, 또한 크레졸분자들이 가용화되는 위치는 미셀의 표면이나 palisade층에서 주로 이루어짐을 알 수 있다.

NaCl Concentration-Dependent Aminoglycoside Resistance of Halomonas socia CKY01 and Identification of Related Genes

  • Park, Ye-Lim;Choi, Tae-Rim;Kim, Hyun Joong;Song, Hun-Suk;Lee, Hye Soo;Park, Sol Lee;Lee, Sun Mi;Kim, Sang Hyun;Park, Serom;Bhatia, Shashi Kant;Gurav, Ranjit;Sung, Changmin;Seo, Seung-Oh;Yang, Yung-Hun
    • Journal of Microbiology and Biotechnology
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    • 제31권2호
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    • pp.250-258
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    • 2021
  • Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, not much information has been available on their antibiotic resistance. In our efforts to learn about the antibiotic resistance of strain Halomonas socia CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the H. socia CKY01 resistance pattern to kanamycin, gentamicin, apramycin, tetracycline, and spectinomycin differed in the presence of 10% NaCl and 1% NaCl in the culture medium. To determine the mechanism underlying this NaCl concentration-dependent antibiotic resistance, we compared four aminoglycoside resistance genes under different salt conditions while also performing time-dependent reverse transcription PCR. We found that the aph2 gene encoding aminoglycoside phosphotransferase showed increased expression under the 10% rather than 1% NaCl conditions. When these genes were overexpressed in an Escherichia coli strain, pETDuet-1::aph2 showed a smaller inhibition zone in the presence of kanamycin, gentamicin, and apramycin than the respective control, suggesting aph2 was involved in aminoglycoside resistance. Our results demonstrated a more direct link between NaCl and aminoglycoside resistance exhibited by the H. socia CKY01 strain.

진도견의 혈액화학치 및 성장에 따른 체형에 관한 연구 I. 진도견의 혈액화학치 (Studies on body types by body growth and blood chemical values in the Jindo dog 1. Blood chemical values of Jindo dogs)

  • 김우권;이재홍;김자숙
    • 대한수의학회지
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    • 제29권3호
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    • pp.263-274
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    • 1989
  • The present study was conducted ill. order to get the normal blood chemical values of Korean Jindo dogs. Blood samples were taken from 160(♂34, ♀126) healthy Jindo dogs in Jindo area. The results obtained in this study were summarized as follows: 1. The mean values of serum total protein(TP), albumin(Alb) and globulin(Glb) content, cholesterol(Chole), magnesium(Mg), calcium(Ca), inorganic phosphate(P), potassium(K), sodium(Na) and chloride(Cl) concentration in the group of less than one year old were 6.64(♂6.62, ♀6.64), 3.63(♂3.57, ♀3.65) and 3.00(♂3.05, ♀2.99)g/100ml, 170.97(♂166.46, ♀172.68)mg/100ml, 1.45(♂1.43, ♀1.46), 5.76(♂5.62, ♀5.81), 4.80(♂4.95, ♀4.75), 4.84(♂4.72, ♀4.89), 148.93(♂148.79, ♀148.98) and 110.22(♂110.42, ♀110.14)mEq/L, respectively, whereas the TP, Alb and Glb content, Chole Mg, Ca, P, K, Na and Cl concentration in the group of one year old and more were 6.88(♂6.84, ♀6.89), 3.65(♂3.63, ♀3.66) and 3.23(♂3.21, ♀3.23)g/100ml, 167.48(♂173.80, ♀166.48)mg/100ml, 1.40(♂1.36, ♀1.40), 5.69(♂5.53, ♀5.71), 4.62(♂4.73, ♀4.60), 4.88(♂4.90, ♀4.87), 149.86(♂149.60, ♀149.90) and 110.03(♂110.70, ♀109.92)mEq/L, respectively. The ratios of mean serum albumin to globulin(A/G), calcium to inorganic phosphate(Ca/P) and sodium to potassium(Na/K) in the group of less than one year old were 1.21(♂1.17, ♀1.22), 1.20(♂1.14, ♀1.22) and 30.77(♂31.52, ♀30.47), respectively, whereas the A/G, Ca/P and Na/K in the group of one year old and more were 1.13(♂1.13, ♀1.13), 1.23(♂1.17, ♀1.24) and 30.71(♂30.53, ♀30.78), respectively. 2. The mean values of Alb content, Mg, Ca and K concentration, A/G and Ca/P ratio appeared to be higher in the female than in the male, whereas the reverse was the case with P concentration. No differences were found between male and female in the TP and Glb content, Chole, Na and Cl concentration and Na/K ratio. 3. The mean values of Glb content appeared to be higher in the group less than one year old than in the group of one year old and more, whereas the reverse was the case with A/G ratio. The mean values of TP and Alb content and Ca/P ratio in the age groups less than one year tended to increase with aging, whereas the reverse was the case with Ca and P concentration. No differences were found between age groups in the Mg, K, Na and Cl concentration and Na/K ratio.

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The Effects of Cement Alkalinity upon the Pore Water Alkalinity and the Chloride Threshold Level of Reinforcing Steel in Concrete

  • ;;김기준
    • 콘크리트학회논문집
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    • 제16권4호
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    • pp.549-555
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    • 2004
  • Cement of three alkalinities (equivalent alkalinities of 0.36,0.52 and 0.97) was employed in fabricating a set of classical G109 type specimens. To-date, these have been subjected to a one week wet-one week dry cyclic pending using 15 w/o NaCl solution. At the end of the dry period, potential and macro-cell current were measured to indicate whether the top reinforcing steel was in the passive or active state. Once this bar became active, the specimen was autopsied and the extent of corrosion was documented. Subsequent to visual inspection, concrete powder samples were collected from the upper region of the top rebar trace; and at a certain times concrete cores were taken from non-reinforced specimens. Using these, determinations were made of (1) critical chloride concentration for corrosion initiation ($Cl_{th}^-$), (2) effective chloride diffusion coefficient ($D_e$), and (3) pore water alkalinity ($[OH^-]$). The pore water alkalinity was strongly related to the alkali content of cement that was used in the mix. The chloride concentration, ($Cl^-$), was greater at active than at passive sites, presumably as a consequence of electro migration and accumulation of these species at active site subsequent to corrosion initiation. Accordingly, ($Cl^-$) at passive sites was considered indicative of the threshold concentration fur corrosion initiation. The $Cl_{th}^-$ was increased with increasing Time-to-corrosion ($T_i$). Consequently, the HA(High Alkalinity) specimens exhibited the highest $Cl_{th}^-$ and the NA(Normal Alkalinity) was the least. This range exceeds what has previously been reported in North America. In addition, the effective diffusion coefficient, $D_e$, was about 40 percent lower for concrete prepared with the HA cement compared to the NA and LA(Low Alkalinity) ones.