• Restorative Dentistry and Endodontics
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• v.24 no.1
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• pp.40-48
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• 1999

This study was designed to examine the specificities of the designed primers for F. nucleatum and F. necrophorum and to compare the PCR results using clinical samples with those of the anaerobic culture method. F. nucleatum and F. necrophorum spp. are frequently isolated in infected root canals, and they are related to periapical diseases. F. nucleatum(VPI 10197) and F. necrophorum(ATCC 25286) were used as references for PCR reaction, and thirty five teeth with one canal and periapical lesion were used. The samples were cultured anaerobically and identified using Rapid ID 32A(BioMerieux Vitek, Inc., France) as biochemical battery. In the GenBank database, species-specific PCR primers(nuc1/nuc2 primers for F. nucleatum and nec1/nec2 primers for F. necrophorum) were designed from the 16S ribosomal DNA(rDNA) sequences of F. nucleatum(accession number M58683) and F. necrophorum(accession number AF044948). PCR procedures of F. nucleatum(VPI 10197) and F. necrophorum (ATCC 25286) were simulated on a computer software. Amplify(v.1.2${\beta}$ for Macintosh). 820 bps and 817 bps of nucleotides were expected, respectively. Using extracted DNAs with QiaAmp tissue kit(Qiagen co., Germany), PCR was done. The results were as follows : 1. The nuc1/nuc2 primers produced an amplicon of 820 bps and the nec1/nec2 primers produced an amplicon of 817 bps. 2. The nuc1/nuc2 primers and the nec1/nec2 primers were specific and did not react with species other than the designated ones(i.e. nuc1/nuc2 primers did not produce amplicons for F. necrophorum, and vice versa.). And the PCR products of Porphyromonas endodontalis(ATCC 35406), Porphyromonas gingivalis(ATCC 33277), Prevotella intermedia(ATCC 25611), and Prevotella nigrescens(ATCC 33563), frequently isolated in infected root canals and periapical lesions, were not amplified by the primers specific for Fusobacterium nucleatum and Fusobacterium necrophorum. 3. This method utilizing PCR could detect F. nucleatum and F. necrophorum in clinical samples, while anaerobic culture method could detect neither.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.586-588
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• 2005

The MSC (Multi Spectral Camera) system is a remote sensing payload to obtain high resolution ground image. This system uses lossy image compression method for &Direct mission& that transmit whole image during one contact. But some image degradation occurred especially at high compression ratio. To reduce this degradation, the MSC uses NUC (Non-uniformity Correction) Unit. This unit correct CCD (Charge Coupled Device)'s high-frequency non-uniformity. So high frequency contents of image can be minimized and whole system SNR can be maximized. But NUC has some disadvantage either. It decreases entire system reliability by adding one electronic system. Adding NUC also led to difficulty of electronic design, assembly and testability. In this paper, the comparison is performed between on-orbit non-uniform correction and on ground correction. by evaluating NUC advantage for the point of view of image quality. Using real MSC parameter and proper model, considerable reference point for the system design came to possible.

• Bulletin of the Korean Chemical Society
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• v.30 no.12
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• pp.2909-2912
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• 2009

The amines studied in this study are less reactive toward ethyl propiolate (3) in MeCN than in H$_2$O although they are 7 to 9 pK$_a$ units more basic in the aprotic solvent. The reactivity of morpholine and deuterated morpholine toward 3 is found to be identical, indicating that proton transfer occurs after rate-determining step (RDS). The fact that kinetic isotope effect is absent excludes a stepwise mechanism in which proton transfer occurs in RDS as well as a concerted mechanism in which nucleophilic attack and proton transfer occur concertedly through a 4-membered cyclic transition state (TS). Thus, the reactions have been concluded to proceed through a stepwise mechanism in which proton transfer occurs after RDS. Brønsted-type plots are linear with small ${\beta}_{nuc}$ values, i.e., ${\beta}_{nuc}$ = 0.29 in H$_2$O and ${\beta}_{nuc}$ = 0.51 in MeCN, indicating that bond formation is not advanced significantly in RDS. The small ${\beta}_{nuc}$ value also supports the conclusion drawn from the study of kinetic isotope effect.

• Korean Journal of Food Science and Technology
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• v.41 no.4
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• pp.423-427
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• 2009

This study investigated antithrombotic and hypocholesterolemic activities of defatted soybean grits (DSG) and fermented DSG (FD). The FD was prepared by the solid state fermentation using Bacillus subtilis NUC1 at $40^{\circ}C$ for 24h. The water extracts of fermented DSG (FDW) exhibited higher fibrinolytic activity and inhibition of platelet aggregation induced by ADP than water extracts of DSG (DW). However, the DW and FDW inhibited HMG-CoA reductase activity and significantly decreased the intracellular cholesterol contents in HepG2 cells. In addition, DW treatment did not show any cholesterol adsorption capacity, while FDW demonstrated the highest cholesterol adsorption by 90%. The results suggest that fermented DSG have significant antithrombotic and hypocholesterolemic effects in vitro and these activities were improved during fermentation by B. subtilis NUC1.

• Journal of Sensor Science and Technology
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• v.17 no.2
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• pp.151-155
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• 2008

In this paper, it was investigated that the pattern recognition of multiplication environment of lactic acid bacteria in the process of curd yogurt preparation using household fermentation system, which was manufactured by combining incubator with sensor module, data processing circuit and computer. It will be sufficiently applicable to determine the maximum ratio of the amount of air to mixed milk for preparation of high quality yogurt.

• Proceedings of the KSRS Conference
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• 2004.10a
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• pp.471-474
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• 2004

Not all CCD pixels generate uniform value for the uniform radiance due to the different process of manufacture and each pixel characteristics. And the image data compression is essential in the real time image transmission because of the high line rate and the limited RF bandwidth. This pixel's nonuniformity and the loss compression make CCD pixel correction necessary in on-orbit condition. In the MSC system, the NUC unit, which is a part of MSC PMU, is charge of the correction for CCD each pixel. The correction is performed with the gain and the offset table for the each pixel and the each TDI mode. These correction tables are generated and programmed in the PMU Flash memory through the various image data tests at the ground test. Besides, they can be uploaded from ground station after onorbit calibration. This paper describes the principle of the table generation and the test way of the non-uniformity after NUC

• Journal of Microbiology
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• v.45 no.4
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• pp.297-304
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• 2007

The detection of virulence factors of Aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. In this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and Aeromonas species were isolated and identified. For rapid detection of six virulence factors of isolated Aeromonas, a hexaplex-polymerase chain reaction (hexaplex-PCR) assay was used. The detected virulence factors include aerolysin (aer), GCAT (gcat), serine protease (ser), nuclease (nuc) lipase (lip) and lateral flagella (laf). The dominant strain found in our isolates was Aeromonas sobria, and the dominant virulence factors were aer and nuc for all seasons. We confirmed that A. sobria and two of the virulence genes (aer and nuc) are related. We proposed a method by which one can identify the major strains of Aeromonas: A. hydrophila, A. sobria, A. caviae, and A. veronii, using hexaplex-PCR.

• Journal of the Korea Society of Computer and Information
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• v.23 no.11
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• pp.1-7
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• 2018

In this paper, we propose soft dead pixels detection method by analysing different response at hot and cold images. Abnormal pixels are able to effect detecting a small target. It also makes confusing real target or not cause of changing target size. Almost exist abnormal pixels after image signal processing even if dead pixels are removed by dead pixel compensation are called soft dead pixels. They are showed defect in final image. So removing or compensating dead pixels are very important for detecting object. The key idea of this proposed method, detecting dead pixels, is that most of soft deads have different response characteristics between hot image and cold image. General infrared cameras do NUC to remove FPN. Working 2-reference NUC must be needed getting data, hot & cold images. The way which is proposed dead pixel detection is that we compare response, NUC gain, at each pixel about two different temperature images and find out dead pixels if the pixels exceed threshold about average gain of around pixels.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.341-343
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• 2014

Camarops ustulinoides (Henn.) Nannf., belonging to the family Boliniaceae (Order Boliniales), is reported as new to Korea. The morphological characteristics and molecular phylogenetic analysis based on the nucLSU region sequence confirm the recognition of this taxon. Illustrated descriptions of C. ustulinoides are provided.

• Aerospace Engineering and Technology
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• v.11 no.1
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• pp.98-102
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• 2012

These days, Uncooled IR Systems are more popular in the area of defense and aerospace than before. Uncooled IR Systems are widely used as core technology for making unmanned systems and detecting enemy objects during the day and night in the distance. Recently, researches on TEC-less IRFPA have been increased to minimize the power consumption and to make a smaller system than before. For this, it needs to find adequate NUC(Non-Uniformity Correction) coefficients as FPA(Focal Plane Array) temperature changes. In this paper, we propose a new NUC coefficient estimating technique, DCCE-LI(Dynamic Calibration Coefficients Estimation with Linear Interpolation), for TEC-less IRFPA. It is based on a linear interpolation method and it can estimate NUC coefficients in real-time. So, by testing and evaluating it with some IR images, we conclude that the quality of IR images using proposed method is better than applying static coefficients.