• Title/Summary/Keyword: NUC

Search Result 120, Processing Time 0.027 seconds

Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples

  • Can, Hayriye Yesim;Elmali, Mehmet;Karagoz, Alper
    • Food Science of Animal Resources
    • /
    • v.37 no.2
    • /
    • pp.175-180
    • /
    • 2017
  • The objectives of this study were: i) to detect the presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Twenty (12.5%) of the samples were found to be contaminated with S. aureus. A total of 40 isolates from the 20 positive samples were confirmed to be S. aureus by multiplex PCR based on 16S rRNA and nuc gene. The mec A gene was not detected in any of the S. aureus strains. In the present study, 39 out of 40 (97.5%) isolates were found to be resistant to one or more antibiotics. All of isolates were susceptible to gentamicin, oxacillin, and vancomycin. The highest resistance rate was detected in penicillin (95%) and ampicillin (92.5%), followed by tetracycline (30%), erythromycin (20%), ciprofloxacin (12.5%). Nine major patterns were determined by PFGE. In 6 of these patterns, thirty-six strains (90%) had identical PFGE profiles.

Effects of Nucleosides and a Nucleotide Mixture on Intenstinal Mucosal Repair in Rats (위축된 흰쥐의 소장 점막의 회복에 미치는 Nucleoside 와 Nucleotide 혼합물의 효과)

  • 이선영
    • Journal of Nutrition and Health
    • /
    • v.31 no.4
    • /
    • pp.679-686
    • /
    • 1998
  • This study was performed to determine whether the infusion of nucleosides and a nucleotide mixture directly ito intestinal lumen can induce a regenerative effect on impaired intestinal mucosa. The effects of massive small bowel resention and also total parenteral nutrition were induced by surgical creation of Thirty-Vella fistual in male Sprague-Dawley rats. The rats received saline solution (Control group) or nucleosides and a nucleotide mixture(lower concentration group(Nucl) or higher concentration group (Nuc2) every two days into the fistula. Mucosal protein, DNA , ornithine decarboxylase(ODC) activity, and morphometry were evaluated at 9 or 21 days postoperation in the fistual and also in the residual ileal segment. On the 9th day, mucosal protein, DNA content, and villous surface area in the fistula and also in the residual ileum increased in rats that received nucleosides and a nucleotide mixture of lower concentration (Nuc 1). On the 21 th day, there were no significant differences in intestinal mucosa between the control group and the lower level nucleoside nucleotide mixture-treated group. The fistula villous height of the higher nucleosides and a nucleotide mixture group was higher than in the control rats. Fistula mucosal ODC activities were not significantly different between groups although the mucosal ODC activity of the residual ileal segment was increased on the 9th day. Our data suggests that this animal model is suitable for studying the effect of dietary factors on intestinal mucosal growth and regeneration after villous stropy , differentiating direct effects of diet on the intestine from systemic effects. It is also suggested that external nucleosides and nucleotides have supportive effects on intestinal mucosal regeneration.

  • PDF

Scene-based Nonuniformity Correction for Neural Network Complemented by Reducing Lense Vignetting Effect and Adaptive Learning rate

  • No, Gun-hyo;Hong, Yong-hee;Park, Jin-ho;Jhee, Ho-jin
    • Journal of the Korea Society of Computer and Information
    • /
    • v.23 no.7
    • /
    • pp.81-90
    • /
    • 2018
  • In this paper, reducing lense Vignetting effect and adaptive learning rate method are proposed to complement Scribner's neural network for nuc algorithm which is the effective algorithm in statistic SBNUC algorithm. Proposed reducing vignetting effect method is updated weight and bias each differently using different cost function. Proposed adaptive learning rate for updating weight and bias is using sobel edge detection method, which has good result for boundary condition of image. The ordinary statistic SBNUC algorithm has problem to compensate lense vignetting effect, because statistic algorithm is updated weight and bias by using gradient descent method, so it should not be effective for global weight problem same like, lense vignetting effect. We employ the proposed methods to Scribner's neural network method(NNM) and Torres's reducing ghosting correction for neural network nuc algorithm(improved NNM), and apply it to real-infrared detector image stream. The result of proposed algorithm shows that it has 10dB higher PSNR and 1.5 times faster convergence speed then the improved NNM Algorithm.

Kinetics and Mechanism for the Reaction of 4-Nitrophenyl 2-Thiophenecarboxylate with Secondary Alicyclic Amines

  • Um, Ik-Hwan;Lee, Eun-Ju;Lee, Jong-Pal
    • Bulletin of the Korean Chemical Society
    • /
    • v.23 no.3
    • /
    • pp.381-384
    • /
    • 2002
  • Second-order-rate constants ($k_N$) have been measured spectrophotometrically for the reactions of 4-nitrophenyl 2-thiophenecarboxylate (1a) with a series of secondary alicyclic amines in H2O containing 20 mole % DMSO at 25.0 $^{\circ}C$ . The ester 1a is less reactive than 4-nitrophenyl 2-furoate (1b) but more reactive than 4-nitrophenyl benzoate (1c) except towards piperazinium ion. The Brønsted-type plots for the aminolyses of 1a, 1b and 1c are linear with a $\beta$nuc value of 0.92, 0.84 and 0.85, respectively, indicating that the replacement of the CH=CH group by a sulfur or an oxygen atom in the benzoyl moiety of 1c does not cause any mechanism change. The reaction of piperidine with a series of substituted phenyl 2-thiophenecarboxylates gives a linear Hammett plot with a large $\rho^-$ value ($\rho^-$ = 3.11) when $\sigma^- $ constants are used. The linear Brønsted and Hammett plots with large $\beta$nuc and $\rho^-$ values suggest that the aminolysis of 1a proceeds via rate-determining break-down of the addition intermediate to the products.

Pyridinolysis of O,O-Diphenyl S-Phenyl Phosphorothiolates in Acetonitrile

  • Adhikary, Keshab Kumar;Lumbiny, Bilkis Jahan;Kim, Chan-Kyung;Lee, Hai Whang
    • Bulletin of the Korean Chemical Society
    • /
    • v.29 no.4
    • /
    • pp.851-855
    • /
    • 2008
  • The reactions of O,O-diphenyl Z-S-phenyl phosphorothiolates with X-pyridines have been studied kinetically in acetonitrile at $35.0{^{\circ}C}$. The Hammett plots for substituent (Z) variations in the leaving group (log $k_2$ vs. $\sigma$ Z) are biphasic concave downwards with breaks at Z = H. The large magnitudes of ${\rho}X(\rho_{nuc})$, ${\beta}X(\rho_{nuc})$, and the cross-interaction constant, $\rho$XZ, suggest frontside nucleophilic attack toward the leaving group. The sign reversal of $\rho$Z from positive in $\sigma$ Z $\leq$ 0 to negative in $\sigma$ Z $\geq$ 0 is interpreted as the change in mechanism from concerted to stepwise with rate-limiting expulsion of the leaving group. The anomalous negative sign of $\rho$ Z for leaving groups with electron-withdrawing substituents is interpreted as the intramolecular ligand exchange process of the leaving group from the equatorial position in the intermediate to the apical position in the TS.

A Kinetic Study on Michael-type Reactions of 1-(X-Substituted Phenyl)-2-propyn-1-ones with Amines: Effect of Amine Nature on Reactivity and Mechanism

  • Um, Ik-Hwan;Hwang, So-Jeong;Lee, Eun-Ju
    • Bulletin of the Korean Chemical Society
    • /
    • v.29 no.4
    • /
    • pp.767-771
    • /
    • 2008
  • Second-order rate constants have been measured spectrophotometrically for the Michael-type reaction of 1-(Xsubstituted phenyl)-2-propyn-1-ones (2a-f) with amines in $H_2O$ at 25.0 ${\pm}$ 0.1 ${^{\circ}C}$. A linear Brønsted-type plot is obtained with ${\beta}_{nuc}$ = 0.25 ${\pm}$ 0.02, a typical $\beta_{nuc}$ value for reactions which proceed through a stepwise mechanism with attack of amine on the electrophilic center being the rate-determining step. Secondary alicyclic amines are found to be more reactive than isobasic primary amines. The Hammett plot for the reactions of 2a-f with morpholine is not linear, i.e., the substrate with a strong electron-donating group (e.g., 4-MeO) exhibits a negative deviation from the Hammett plot. However, the Yukawa-Tsuno plot for the same reactions exhibits an excellent linear correlation with ρ = 0.62 and r = 0.82. Thus, it has been proposed that the nonlinear Hammett plot is not due to a change in the ra te-determining step but due to ground-state stabilization through resonance interactions.

IR Image Processing IP Design, Implementation and Verification For SoC Design

  • Yoon, Hee-Jin
    • Journal of the Korea Society of Computer and Information
    • /
    • v.23 no.1
    • /
    • pp.33-39
    • /
    • 2018
  • In this paper, We studied the possibility of SoC(System On Chip) design using infrared image processing IP(Intellectual Property). And, we studied NUC(Non Uniformity Correction), BPR(Bad Pixel Recovery), and CEM(Contrast Enhancement) processing, the infrared image processing algorithm implemented by IP. We showed the logic and timing diagram implemented through the hardware block designed based on each algorithm. Each algorithm was coded as RTL(Register Transfer Level) using Verilog HDL(Hardware Description Language), ALTERA QUARTUS synthesis, and programed in FPGA(Field Programmable Gated Array). In addition, we have verified that the image data is processed at each algorithm without any problems by integrating the infrared image processing algorithm. Particularly, using the directly manufactured electronic board, Processor, SRAM, and FLASH are interconnected and tested and the verification result is presented so that the SoC type can be realized later. The infrared image processing IP proposed and verified in this study is expected to be of high value in the future SoC semiconductor fabrication. In addition, we have laid the basis for future application in the camera SoC industry.

Antioxidant Activity and Anti-hyperglycemic Activity of Medicinal Herbal Extracts According to Extraction Methods (약용식물의 추출방법에 따른 항산화 및 항당뇨 활성)

  • Jeong, Hyun-Jin;Lee, Sung-Gyu;Lee, Eun-Ju;Park, Woo-Dong;Kim, Jong-Boo;Kim, Hyun-Jeong
    • Korean Journal of Food Science and Technology
    • /
    • v.42 no.5
    • /
    • pp.571-577
    • /
    • 2010
  • Korean traditional medicinal herbs have been reported to possess antioxidant and anti-hyperglycemic activities. We tested the antioxidant and anti-hyperglycemic activities of 6 kinds of medicinal herbs: Angelica gigas N., Poria cocos, Mori radicis Cortex, Mori folium, Aralia elata Cortex, and Panax ginseng, prepared as hot water, ethanol, and sonication extracts. The antioxidant activities of the extracts were examined by performing total polyphenol, total flavonoid, and ${\alpha}$,${\alpha}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) assays. For M. folium, the ethanol extract showed the strongest effects in DPPH radical scavenging activity among the three extraction methods. In addition, sonication extracts of M. radicis Cortex and M. folium showed the highest inhibitory activities for ${\alpha}$-glucosidase among the different extracts. The ethanol extracts of M. folium had the highest inhibition effects against ${\alpha}$-amylase. A direct correlation between antioxidant and anti-hyperglycemic inhibition activity was found in the ethanol and sonication extracts. From the results, it is considered that these six medicinal herbal extracts have antioxidative, anti-hyperglycemic, and correlation effects based on different extraction methods.

Cholesterol Improvement Synergistic Effects of Fermented Soybean Grits Caused by Added with Mung Bean in vitro (녹두 첨가로 인한 탈지대두 Grits(Defatted Soybean Grits) 발효물의 in vitro 상에서의 콜레스테롤 개선능 상승효과)

  • Lee, Sung-Gyu;Kim, Hyun-Jeong;Yu, Mi-Hee;Lee, Eun-Ju;Lee, Sam-Pin;Lee, In-Seon
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.39 no.7
    • /
    • pp.947-952
    • /
    • 2010
  • This study was performed to investigate cholesterol improvement of fermented defatted soybean grits (FD) and FD added with 2.5, 5, 10% mung bean (FDM). The FD and FDM were prepared by the solid state fermentation using Bacillus subtilis NUC1 at $40^{\circ}C$ for 24 hr. More than 70% cholesterol adsorption of FD and FDM groups was shown. Particularly, FDM added with 2.5% mung bean (2.5% FDM) showed highest cholesterol adsorption by 90% among FD and FDM groups. 2.5% FDM showed 42% inhibition effect on HMG-CoA reductase, and significantly decreased the intracellular cholesterol contents in HepG2 cells. Apolipoprotein AI, CIII improvement effects of FD and FDM group in HepG2 cells showed most effects in the 2.5% FDM. The results suggest that FDM added with 2.5% mung bean may be beneficial to the prevention of hypercholesterol.

Antioxidant and Anticancer Activities of Defatted Soybean Grits Fermented by Bacillus subtilis NUC1 (고초균에 의한 탈지대두 Grits 발효물의 항산화 및 항암 활성)

  • Lee, Sung-Gyu;Kim, Hyun-Jeong;Lee, Sam-Pin;Lee, In-Seon
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.38 no.6
    • /
    • pp.657-662
    • /
    • 2009
  • Antioxidant and anticancer activities of water and ethanol extracts of defatted soybean grits (DSG) fermented Bacillus subtilis NUC1 were determined and compared with those of the raw DSG. The fermented defatted soybean grits (FDSG) exhibited higher total polyphenols and flavonoids contents than DSG. The ethanol extracts of FDSG (FD-E) showed the highest polyphenol and flavonoid contents with 23.35 mg/g and 3.48 mg/g, respectively. Particularly, FD-E showed higher DPPH radical scavenging activities with $RC_{50}$ of 0.32 mg/mL than other samples with $RC_{50}$ of 1.10${\sim}$3.89 mg/mL. The water and ethanol extracts of FDSG and DSG showed growth inhibitory effects against AGS, A549 and Hela cancer cells in a dose-dependent manner, and especially FD-E showed the highest growth inhibition effects. FD-E induced apoptosis in Hela cells through an increased activation of caspase-3 and caspase-3 target protein, PARP, but rarely affected caspase-7.