• 전기전자학회논문지
• /
• 제27권4호
• /
• pp.650-656
• /
• 2023

본 논문은 NS-3 기반의 5G 시스템 레벨 시뮬레이터를 활용하여 mmWave 대역의 채널 모델을 구현하고, RF 성능 검증을 통해 구현한 모델의 신뢰성과 유효성을 분석한다. mmWave 대역의 RF 성능 검증을 위한 채널 모델은 3GPP TR 38.901에서 정의하는 채널의 특성, 빔포밍, 안테나 구성, 시나리오 등의 파라미터를 고려하여 구성한다. 또한, 시뮬레이션 결과는 3GPP 표준에서 허용하는 범위 내에 있음을 확인하고, REM(Radio Environment Map)을 활용하여 실내 환경 시나리오에서 신뢰성을 증명한다. 따라서 본 연구에서 구현한 채널 모델은 실제 5G 네트워크의 설계 및 구축에 적용이 가능하며, 다양한 파라미터 변경으로 RF 성능을 평가하고 검증하는 방법을 제시한다.

• 한국정보통신학회논문지
• /
• 제17권3호
• /
• pp.584-596
• /
• 2013

WSN(Wireless Sensor Network)를 활용하는 범위가 점차 확대 되면서 TCP/IP 기반의 공용 네트워크에서 WSN에 접근하고자 하는 요구가 증가 하였고, 이로 인해 이 네트워크 환경 모델을 테스트하기 위한 연구가 꾸준히 진행 되었다. 하지만 다수의 센서 노드들을 필요로 하는 WSN을 위한 테스트 베드를 구축하는 것은 매우 어려운 일이다. 본 논문에서는 이러한 네트워크 환경 모델을 테스트베드와 NS-3를 통합한 테스트 시뮬레이션 환경을 제공한다. 뿐만아니라 테스트베드의 시뮬레이션 시간도 NS-3의 리얼타임 스케줄러를 이용하여 통합할 수 있는 시뮬레이션 환경을 제공한다.

• Molecules and Cells
• /
• 제43권5호
• /
• pp.469-478
• /
• 2020

Hepatitis C virus (HCV) propagation is highly dependent on cellular proteins. To identify the host factors involved in HCV propagation, we previously performed protein microarray assays and identified the LIM and SH3 domain protein 1 (LASP-1) as an HCV NS5A-interacting partner. LASP-1 plays an important role in the regulation of cell proliferation, migration, and protein-protein interactions. Alteration of LASP-1 expression has been implicated in hepatocellular carcinoma. However, the functional involvement of LASP-1 in HCV propagation and HCV-induced pathogenesis has not been elucidated. Here, we first verified the protein interaction of NS5A and LASP-1 by both in vitro pulldown and coimmunoprecipitation assays. We further showed that NS5A and LASP-1 were colocalized in the cytoplasm of HCV infected cells. NS5A interacted with LASP-1 through the proline motif in domain I of NS5A and the tryptophan residue in the SH3 domain of LASP-1. Knockdown of LASP1 increased HCV replication in both HCV-infected cells and HCV subgenomic replicon cells. LASP-1 negatively regulated viral propagation and thereby overexpression of LASP-1 decreased HCV replication. Moreover, HCV propagation was decreased by wild-type LASP-1 but not by an NS5A binding-defective mutant of LASP-1. We further demonstrated that LASP-1 was involved in the replication stage of the HCV life cycle. Importantly, LASP-1 expression levels were increased in persistently infected cells with HCV. These data suggest that HCV modulates LASP-1 via NS5A in order to regulate virion levels and maintain a persistent infection.

• 한국식품조리과학회지
• /
• 제14권4호
• /
• pp.380-387
• /
• 1998

The stability of red pepper seed oils during storage at 20${\pm}$3$^{\circ}C$, 40${\pm}$3$^{\circ}C$ and heating at 140${\pm}$3$^{\circ}C$ or 180${\pm}$3$^{\circ}C$ were measured to evaluate red pepper seed oil as a cooking oil. Two species of red pepper seeds (native, improved) were dried by either sunlight or heating to prepare the oil samples of NS (native, sunlight-dried), IS (improved, sunlight-dried), NF (native, heated), and IF (improved, heated). During storage at 20${\pm}$3$^{\circ}C$ or 40${\pm}$ 3$^{\circ}C$, acid values (AV) of all red pepper seed oils were higher than that of soy bean oil (SBO), however, peroxide values (POV) were similar to SBO. Antioxidative stability of NS was better than SBO but IF was not. By the heat treatments at 140${\pm}$3$^{\circ}C$ or 180${\pm}$3$^{\circ}C$, NS was identified to have better antioxidative stability than SBO and IF was the lowest. In sensory evaluation of each deep-fat fried potato-chip at 180${\pm}$5$^{\circ}C$, potato-chips fried in NS were better than that of SBO for color, taste, and flavor. NS-fried potato-chips got the highest score in overall acceptance (p<0.05), however, those of IF showed little acceptance. When blended oils (SBO: NOS, 0, 25, 50, 75%) were used, 50% blended oil was the best for taste, color, flavor, cripness, and total acceptance.

• Journal of Microbiology and Biotechnology
• /
• 제29권10호
• /
• pp.1665-1674
• /
• 2019

Zika virus (ZIKV) is a mosquito-transmitted, emerging Flavivirus that causes Guillain-$Barr{\acute{e}}$ syndrome and microcephaly in adults and fetuses, respectively. Since ZIKV was first isolated in 1947, severe outbreaks have occurred at various places worldwide, including Yap Island in 2007, French Polynesia in 2013, and Brazil in 2015. Although incidences of ZIKV infection and dissemination have drastically increased, the mechanisms underlying the pathogenesis of ZIKV have not been sufficiently studied. In addition, despite extensive research, the exact roles of individual ZIKV genes in the viral evasion of the host innate immune responses remain elusive. Besides, it is still possible that more than one ZIKV-encoded protein may negatively affect type I interferon (IFN) induction. Hence, in this study, we aimed to determine the modulations of the IFN promoter activity, induced by the MDA5/RIG-I signaling pathway, by over-expressing individual ZIKV genes. Our results show that two nonstructural proteins, NS2A and NS4A, significantly down-regulated the promoter activity of IFN-${\beta}$ by inhibiting multiple signaling molecules involved in the activation of IFN-${\beta}$. Interestingly, while NS2A suppressed both full-length and constitutively active RIG-I, NS4A had inhibitory activity only on full-length RIG-I. In addition, while NS2A inhibited all forms of IRF3 (full-length, regulatory domain-deficient, and constitutively active), NS4A could not inhibit constitutively active IRF3-5D. Taken together, our results showed that NS2A and NS4A play major roles as antagonists of MDA5/RIG-I-mediated IFN-${\beta}$ induction and more importantly, these two viral proteins seem to inhibit induction of the type I IFN responses in differential mechanisms. We believe this study expands our understanding regarding the mechanisms via which ZIKV controls the innate immune responses in cells and may pave the way to development of ZIKV-specific therapeutics.

• Biomolecules & Therapeutics
• /
• 제27권5호
• /
• pp.457-465
• /
• 2019

Patients with diabetes mellitus (DM) often suffer from diverse skin disorders, which might be attributable to skin barrier dysfunction. To explore the role of lipid alterations in the epidermis in DM skin disorders, we quantitated 49 lipids (34 ceramides, 14 free fatty acids (FFAs), and cholesterol) in the skin epidermis, liver, and kidneys of db/db mice, a Type 2 DM model, using UPLC-MS/MS. The expression of genes involved in lipid synthesis was also evaluated. With the full establishment of hyperglycemia at the age of 20 weeks, remarkable lipid enrichment was noted in the skin of the db/db mice, especially at the epidermis and subcutaneous fat bed. Prominent increases in the ceramides and FFAs (>3 fold) with short or medium chains ($LXR{\alpha}/{\beta}$ and $PPAR{\gamma}$, nuclear receptors promoting lipid synthesis, lipid synthesis enzymes such as elongases 1, 4, and 6, and fatty acid synthase and stearoyl-CoA desaturase were highly expressed in the skin and livers of the db/db mice. Collectively, our study demonstrates an extensive alteration in the skin and systemic lipid profiles of db/db mice, which could contribute to the development of skin disorders in DM.

• Composites Research
• /
• 제25권5호
• /
• pp.141-146
• /
• 2012

본 논문에서는 Tsai-Wu 파손이론을 적용하여 Carbon/Epoxy 복합재 적층판으로 이루어진 자전거 휠의 경량화를 위한 적층수와 적층각에 대한 강도설계에 대하여 연구하였다. 복합재료는 적층수와 적층각에 따라 강도와 강성이 변하기 때문에 적용되는 설계 하중 조건에 따른 구조물의 최취약부에서 파손이 일어나지 않도록 최적의 적층각과 최소의 적층수를 찾아야한다. 따라서 유한요소해석을 이용하여 복합재 자전거 휠에 부가되는 수직, 복합하중조건에 대하여 가장 안정적인 적층수 및 적층각을 구하였다. 휠에 적용되는 적층각은 $[0]_{8n}$, $[90]_{8n}$, $[0/90]_{2ns}$, $[{\pm}45]_{2ns}$, $[0/{\pm}45/90]_{ns}$으로 적층수를 변화시켜가며 유한요소해석을 수행하였다. 해석 결과로부터 $[0/{\pm}45/90]_{3s}$의 적층각이 파손에 안정적인 적층각 임을 제안하였다. 또한 이를 통하여 휠의 최취약부를 알 수 있었다.

• 한국컴퓨터정보학회논문지
• /
• 제7권3호
• /
• pp.135-142
• /
• 2002

본 논문에서는 데이터 상관기들의 증가로 인한 데이터 처리시간 지연을 감소시키기 위해 Walsh Switch, 공유 accumulator, 그리고 파이프라인 FWHT 알고리즘을 적용한 새로운 Rake Finger 구조를 제안했다. 모의실험 결과, 왈쉬 코드 채널의 수 N＝4에 대한 데이터 상관기의 연산 동작 수는 512 additions에서 160 additions로 약 3.2배 감소하였고, Rake Finger의 데이터 처리시간은 110,696(ns)에서 90,496(ns)로 18.3% 감소하였음을 확인하였다.

• The Korean Journal of Pain
• /
• 제24권3호
• /
• pp.131-136
• /
• 2011

Background: Pregabalin is an anticonvulsant and analgesic agent that interacts selectively with the voltage-sensitive-$Ca^{2+}$-channel alpha-2-delta subunit. The aim of this study was to evaluate whether the analgesic action of intrathecal (IT) pregabalin is associated with KATP channels in the rat formalin test. Methods: IT PE-10 catheters were implanted in male Sprague-Dawley rats (250.300 g) under inhalation anesthesia using enflurane. Nociceptive behavior was defined as the number of hind paw flinches during 60 min after formalin injection. Ten min before formalin injection, IT drug treatments were divided into 3 groups: normal saline (NS) $20\;{\mu}l$ (CON group); pregabalin 0.3, 1, 3 and $10\;{\mu}g$ in NS $10\;{\mu}l$ (PGB group); glibenclamide $100\;{\mu}g$ in DMSO $5\;{\mu}l$ with pregabalin 0.3, 1, 3 and $10\;{\mu}g$ in NS $5\;{\mu}l$ (GBC group). All the drugs were flushed with NS $10\;{\mu}l$. Immunohistochemistry for the $K_{ATP}$ channel was done with a different set of rats divided into naive, NS and PGB groups. Results: IT pregabalin dose-dependently decreased the flinching number only in phase 2 of formalin test. The log dose response curve of the GBC group shifted to the right with respect to that of the PGB group. Immunohistochemistry for the $K_{ATP}$ channel expression on the spinal cord dorsal horn showed no difference among the groups 1 hr after the formalin test. Conclusions: The antinociceptive effect of pregabalin in the rat formalin test was associated with the activation of the $K_{ATP}$ channel. However, pregabalin did not induce $K_{ATP}$ channel expression in the spinal cord dorsal horn.

• 미생물학회지
• /
• 제43권3호
• /
• pp.159-165
• /
• 2007

C형 간염바이러스(hepatitis C virus; HCV)증식을 효과적이며 특이적으로 제어할 수 있는 유전산물을 개발하기 위하여 HCV 중식조절이자인 NS5B RNA replicase 존재에 의해 allosteric하게 그 활성 이 조절될 수 있는 HCV internal ribosome entry site (IRES) 표적 hammerhead 리보자임을 개발하였다. 우선 HCV IRES 염기서열 중+382 nucleotide(nt) 부위가 리보자임에 의해 가장 잘 인식되었음을 관찰하였다. 이러한 allosteric 리보자임은 NS5B RNA replicase와 특이적으로 결합하는 RNA aptamer 부위, aptamer와 NS5B와의 결합에 의해 리보자임 활성을 유도할 수 있도록 구조적 변이를 전달할 수 있는 communication module부위 및 HCV IRES의 +382 nt를 인지하는 hammerhead 리보자임 등으로 구성되도록 설계하였다. 특히 in vitro selection기법을 활용하여 NS5B 의존적으로 리보자임 활성을 증가시킬 수 있는 communication module 염기서열을 밝혀내었다. 이러한 리보자임은 단백질이 없거나 대조 단백질인 bovine serum albumin이 존재할 때에는 절단반응을 유도하지 못하였으나 HCV NS5B 단백질이 존재할 매에만 효과적으로 NS5B 농도 의존적으로 절단 반응을 유도할 수 있음을 관찰하였다. 이러한 allosteric 리보자임은 HCV중식의 효과적인 증식 억제 선도물질 뿐만 아니라 HCV 치료선도물질의 스크리닝용 도구 및 HCV 조절 인자를 탐색할 수 있는 HCV 진단용 리간드로서도 활용될 수 있을 것이다.