• Title/Summary/Keyword: NR4A2

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A Study on the Antimicrobial Activity and in vitro Cytotoxicity of UVB Sunscreen Chemicals in Cosmetic Products (UVB 자외선 차단제의 항균력 및 피부자극에 관한 연구)

  • 최종완;허윤석;손근욱
    • Proceedings of the SCSK Conference
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    • 1992.09a
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    • pp.46-68
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    • 1992
  • To investigate the effect on the antimicrobial activity against S.aureus ATCC 6538, E.coli KCTC 1039 and cell toxic level against transformed mouse fibroblast L929 in formula added with various concentrations of UVB blockers commonly used in cosmetic products, these experiments were carried out by preservative efficacy testing methods and in vitro cytotoxicity methods. The results obtained were as follow ; 1) Octyl Dimethyl PABA had a broad antibacterial spectrum against the Gram (+) and the Gram(-) bacteria at 5.84 % concentration, but not Octyl Methoxycinnamate. 2) Antibacterial activity was decreased in a combined UVB blocker system of squalane base. Especially, Octyl Dimethyl PABA was inactivated by Octyl Methoxycinnamate at 5.84% concentration to a large extents , but not 4-Methylbenzylidene Camphor. 3) Within in vitro cytotoxicity by use of mouse fibroblast L929 on UV-B blockers, NR assay was more excellent than MTT assay on quantitative

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Heat and Crack Resistance of Natural Rubber(NR) Compounds According to the Type of Antioxidants (산화방지제 종류에 따른 천연고무 배합물의 내열성 및 내크랙성)

  • Roh, Jong-Dae;Shin, Jun-Geun;Kim, Jin-Tae;Hur, Jae-Young;Kim, Won-Ho
    • Elastomers and Composites
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    • v.34 no.4
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    • pp.341-349
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    • 1999
  • In this study, heat and crack resistance of natural rubber (NR) compounds was evaluated. To prevent the effects of the crosslinking system, a conventional vulcanization system was selected, where the accelerator/sulfur ratio was fixed to 0.25. Vulcanizates containing phenylenediamine showed high tensile strength and tear strength compared to other vulcanizates because phenylenediamine can cause additional crosslinking and high dispersion In the vulcanizates. In the pure shear test, vulcanizates containing phenylenediamine showed an excellent tearing energy which was due to the irregular crack path, and showed excellent heat and crack resistance which was also due to the good dispersity of antioxidant and additional crosslinks in the rubbber vulcanizates.

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Effects of Herbar Chelidonii on the Cultured Spinal Sensory Neurons Damaged by XO/HX (백굴채(白屈菜)가 손상된 배양척수감각신경세포에 미치는 영향)

  • Shin, Byung-Cheul;Song, Yung-Sun
    • The Journal of Korea CHUNA Manual Medicine
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    • v.2 no.1
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    • pp.143-157
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    • 2001
  • Objectives and Methods : To evaluate the mechanism of oxidative damage by xanthine oxydase(XO) and hypoxanthine(HX)-induced oxygen radicals, MTT assay and NR assay were carried out after the cultured mouse spinal sensory neurons were preincubated for 4 hours with various concentrations of XO/HX. And the amount of total protein. neurofilament EIA. lipid peroxidation and LDH activity were measured, to evaluate the protective effect of Herbar Chelidonii(HC) water extract on cultured spinal sensory neurons damaged by XO/HX. after the cultured mouse spinal sensory neurons were preincubated with various concentrations of HC water extract for 3 hours prior to exposure of XO/HX. Results : XO/HX decreased significantly the survival rate of the cultured mouse sensory neurons by NR assay and MTT assay In proportion to concentration and exposed time. In proportion to concentration and exposed time on cultured spinal sensory neurons, XO/HX showed the quantitative decrease of neurofilament by EIA. the decrease of total protein amount by SRB assay and the Increase of lipid peroxidation as well as LDH. HC showed the quantitative increase of neurofilament and total protein, but showed the decrease of lipid peroxidation and LDH activity against the neurotoxicity of XO/HX. Conclusions : From the above results, it is concluded that XO/HX have a neurotoxic effect on cultured spinal sensory neurons and that the herbs extract, such as HC, prevent the toxicity of XO/HX effectively in that they decrease lipid peroxidation and LDH activity.

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Influence of Mixing Procedure on Properties of Carbon Black-filled Natural Rubber Compounds

  • Park, Sung-Seen
    • Macromolecular Research
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    • v.8 no.4
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    • pp.192-198
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    • 2000
  • Cure characteristics and physical properties of carbon black-filled natural rubber (NR) compounds depending on the mixing procedure were studied using the compounds with different pre-final mixing (FM-1) stages. Carbon master batch (MB) and first and second remitting (1RM and 2RM) stages were employed as the FM-1 stage. Bound rubber content of the FM compound decreased with increasing the mixing steps. This was due to the decrease of the molecular weight distribution of the polymer by the rubber chain scission during the mixing. The Mooney viscosity decreased with increasing the mixing steps. Cure characteristics of the compounds were found to be different with the mixing procedures. The cure times of the compound became slower by increasing the number of the mixing steps. This was explained by the length of rubber chain, the carbon black network, distribution of the curatives in the compound, and immobilization of the polymeric segments. Modulus and tensile strength of the vulcanizate did not show a specific trend with the mixing procedure. Fatigue life of the vulcanizate increased by increasing the mixing stages.

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Nitrogenase Derepression and Associated Metabolism in a Microaerophilic Cyanobacterium, Plectonema boryanum

  • Pandey, Kapil Deo;Sukla, Sarkar;Naz, Shaheen;Smita, Chaturvedi;Ajaikumar, Kashyap
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.179-185
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    • 2001
  • Nitrate grown cells of cyanobacterium Plectonema boryanum, transferred to nitrogen stress, evolved nitrogenase catalyzed $H_2$ under microaerophilic condition. Nitrogen ($N_2$) in gs phase, low light intensity, and reducing substances in incubation phase stimulated $N_2$fixation ($H_2\;evolution$). Cyanobacterium grew slowly under microaerobic condition with a low intracellular ammonia pool. Nitrogen sources (${NO_3}^-,{NH_4}^+,\;and\;CH_3NH_3$) inhibited nitrogenase and glutamine synthetase (GS) transferase activity, and methylamine behaved like an ammonical nitrogen source. Depletion of molybdenum (Mo) and addition of tungsten (W) in the incubation medium inhibited $H_2$ evolution, Cyanobacterium was able to take up nitrate and expressed nitrate reductase (NR) activity under microaerophilic condition at an extremely slow rate.

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Synthesis and Exploitation in Solar Cells of Hydrothermally Grown ZnO Nanorods Covered by ZnS Quantum Dots

  • Mehrabian, Masood;Afarideh, Hossein;Mirabbaszadeh, Kavoos;Lianshan, Li;Zhiyong, Tang
    • Journal of the Optical Society of Korea
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    • v.18 no.4
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    • pp.307-316
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    • 2014
  • Improved power conversion efficiency of hybrid solar cells with ITO/ZnO seed layer/ZnO NRs/ZnS QDs/P3HT/PCBM/Ag structure was obtained by optimizing the growth period of ZnO nanorods (NRs). ZnO NRs were grown using a hydrothermal method on ZnO seed layers, while ZnS quantum dots (QDs) (average thickness about 24 nm) were fabricated on the ZnO NRs by the successive ionic layer adsorption and reaction (SILAR) technique. Morphology, crystalline structure and optical absorption of layers were analyzed by a scanning electron microscope (SEM), X-ray diffraction (XRD) and UV-Visible absorption spectra, respectively. The XRD results implied that ZnS QDs were in the cubic phase (sphalerite). Other experimental results showed that the maximum power conversion efficiency of 4.09% was obtained for a device based on ZnO NR10 under an illumination of one Sun (AM 1.5G, $100mW/cm^2$).

Effect of Resistance Training on Skeletal Muscle Gene Expression in Rats: a Beadarray Analysis (저항성 운동이 골격근 유전자 발현에 미치는 영향: Beadarray 분석)

  • Oh, Seung-Lyul;Oh, Sang-Duk
    • Journal of Life Science
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    • v.23 no.1
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    • pp.116-124
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    • 2013
  • The aim was to examine resistance exercise-related genes after 8 weeks of resistance training. Thirty-two male Sprague-Dawley rats were divided into four groups: 4 weeks sedentary (4 wks CON, n=8), 8 weeks sedentary (8 wks CON, n=8), 4 weeks exercise training (4 wks REG, n=8), and 8 weeks exercise training (8 wks REG, n=8). The rats were trained to climb a 1-m vertical incline (85-degree), with weights secured to their tails. They climbed 10 times, 3 days per week, for 8 consecutive weeks. Skeletal muscle was taken from the flexor halucis longus after the exercise training. After separating the total RNA, large-scale gene expression was investigated by beadarray (Illumina RatRef-12 Expression BeadChip) analysis, and qPCR was used to inspect the beadarray data and to analyze the RNA quantitatively. The detection p-value for the genes was p<0.01, the M-value {M=$log_2$(condition)-$log_2$(reference)} was >1.0, and the DiffScore was >20. In total, the expression of 30 genes significantly increased 4 weeks after the exercise training, and the expression of six genes decreased. At 8 weeks, the expression of five genes significantly increased and that of 12 decreased. Several genes are potentially involved in resistance exercise and muscle hypertrophy, including 1) regulation of cell growth (IGFBP1, PLA2G2A, OKL38); 2) myogenesis (CSRP3); 3) tissue regeneration and muscle development (MUSTN1, MYBPH); 4) hypertrophy (CYR61, ATF3, NR4A3); and 5) glucose metabolism (G6PC, PCK1). These results may help to explain previously reported physiological changes of the skeletal muscle and suggest new avenues for further investigation.

Phylogenetic study of the section Adonanthe of genus Adonis L. (Ranunculaceae) based on ITS sequences (ITS 염기서열에 의한 복수초속 복수초절(미나리아재비과)의 계통분류학적 연구)

  • Son, Dong Chan;Park, Beom Kyun;Ko, Sung Chul
    • Korean Journal of Plant Taxonomy
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    • v.46 no.1
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    • pp.1-12
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    • 2016
  • DNA sequences of nrITS regions obtained from 49 accessions representing 12 species and one variety within the section Adonanthe of the genus Adonis were analyzed to test the previous intra-sectional classification system and to determine their phylogenetic relationships. The results showed that the seven accessions of A. amurensis Regel et Radde included in the present study did not form a monophyletic group, as some of the accessions showed a close relationship with the Japanese endemic species A. ramosa Franch., which implies that the current species delimitation and identification of A. amurensis is problematic. Adonis pseudoamurensis W. T. Wang, which is frequently misidentified as A. ramosa in Korea, formed a segregated group, which suggests that they should not be considered as conspecific taxa. Accessions from A. shikokuensis Nishikawa et Koji Ito, A. multiflora Nishikawa et Koji Ito, and A. pseudoamurensis formed a clade, but monophyly of each species was not evident. The nrITS data did not support the classification system proposed by Wang, who classified sect. Adonanthe into four series, as most of these were found to be either polyphyletic or paraphyletic.

A Network QoS Model for Joint Integrated C4I Structure (합동지휘통제 통합망 구조 QoS 모델(안))

  • Park, Dongsuk;Oh, Donghan;Choi, Eunho;Lim, Jaesung
    • Journal of the Korea Institute of Military Science and Technology
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    • v.23 no.2
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    • pp.106-114
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    • 2020
  • NCW which is shaping favorable conditions with obtaining initiative through superiority in C2 and information sharing is critical to the result of the war in a modern warfare. An important requirement to attain superiority through an effective networking in a war-environment is to apply QoS to ensure priority in supporting critical mission and services. In order to obtain an effective NCOE through JCS-led QoS support, standard doucments have been reviewed and analyzed to understand the current level of technology and development. In addition, QoS-related policy documents which is currently being applied by the ROK armed forces have been analyzed to substantiated the JCS-led QoS model and propose the directions of development and enhancement required in the realm of technology, policy and system.

Effect of Radish Leaves Powder on the Gastrointestinal Function and Fecal Triglyceride, and Sterol Excretion in Rats Fed a Hypercholesterolemic Diet (무청분말이 콜레스테롤식이를 공급한 흰쥐의 장기능 및 분변 중 중성지질 및 Sterol 배설에 미치는 영향)

  • Jang, Hyun-Seo;Ahn, Jung-Mo;Ku, Kyung-Hyung;Rhee, Soon-Jae;Kang, Shin-Kwon;Choi, Jeong-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.10
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    • pp.1258-1263
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    • 2008
  • The current study examined the effects of radish leaves powder on the excretion of fecal triglyceride, and sterol and hepatic UDP-glucuronyl transferase (UDPGT) activity in rats fed hypercholesterolemic diet. Male Sprague-Dawley rats weighing 100$\pm$10 g were randomly assigned to normal control group (N group), normal diet with 5% radish leaves powder supplemented group (NR) and hypercholesterolemic groups, which were sub-divided into radish leaves powder free diet group (HC) and 2.5% (HRL), 5% (HRM), and 10% (HRH) radish leaves powder supplemented groups. The experimental diets were fed ad libitum for 4 weeks. Fecal weights and water contents were significantly increased in all radish leaves powder supplemented groups (NR, HRL, HRM, and HRH) than that of N and HC groups. Fecal total lipid contents including fecal neutral and acidic sterols in radish leaves powder supplemented groups were higher than those of the HC group, and especially that of HRH group was the highest among all experimental groups. Hepatic UDPGT activity of HRH group was 38% higher than that of HC group. Excretions of fecal bile acid were increased 2.3 and 2.7 folds in HRM and HRH groups compared with that of HC group. And neutral sterol, coprostanol, and coprostanone contents of them were higher in radish leaves supplemented groups than in HC group. These results suggest that radish leaves may act as potential substitute for a dietary fiber capable of improving a gastrointestinal function and lipid metabolism.