• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.212-217
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• 2011

A photoautotrophic micropropagation methodology in liquid culture medium has a number of advantages for large-scale propagation of plants. This paper describes an improved system for the mass propagation via somatic embryogenesis of the medicinal plant Kalopanax septemlobus Nakai. Somatic embryo-derived young plantlets of K. septemlobus were cultured either under heterotrophic conditions with sucrose on half-strength MS medium with $30gL^{-1}$ sucrose, under heterotrophic conditions without sucrose, or under photoautotrophic conditions (MS liquid medium without sucrose under forced aeration) for four weeks before transferring the plantlets for acclimatization. Plantlets grown under photoautotrophic conditions had more leaves, higher chlorophyll content, a higher net photosynthetic rate (NPR), and a higher survival rate. The results indicate that the photoautotrophic conditions with a forced ventilation system are effective in enhancing the growth of plantlets and the rate of net photosynthesis. The plantlets grown under photoautotrophic conditions had a high survival rate (92%) upon ex vitro transplantation. Our study shows that autotrophically produced plantlets acclimatize better and sooner upon ex vitro transplantation than conventionally cultured plants.

• Molecules and Cells
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• v.25 no.4
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• pp.559-565
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• 2008

Members of the TGA family of basic domain/leucine zipper transcription factors regulate defense genes through physical interaction with NON-EXPRESSOR OF PR1 (NPR1). Of the seven TGA family members, TGA4/octopine synthase (ocs)-element-binding factor 4 (OBF4) is the least understood. Here we present evidence for a novel function of OBF4 as a regulator of flowering. We identified CONSTANS (CO), a positive regulator of floral induction, as an OBF4-interacting protein, in a yeast two-hybrid library screen. OBF4 interacts with the B-box region of CO. The abundance of OBF4 mRNA cycles with a 24 h rhythm under both long-day (LD) and short-day (SD) conditions, with significantly higher levels during the night than during the day. Electrophoretic mobility shift assays revealed that OBF4 binds to the promoter of the FLOWERING LOCUS T (FT) gene, a direct target of CO. We also found that, like CO and FT, an OBF4:GUS construct was prominently expressed in the vascular tissues of leaf, indicating that OBF4 can regulate FT expression through the formation of a protein complex with CO. Taken together, our results suggest that OBF4 may act as a link between defense responses and flowering.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.1
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• pp.73-80
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• 2018

In motorway traffic flow control, the conventional way based on real-time response has been changed into advanced way based on proactive response. Future traffic conditions over multiple time intervals are crucial input data for advanced motorway traffic flow control. It is necessary to overcome the uncertainty of the future state in order for forecasting multiple-period traffic volumes, as the number of uncertainty concurrently increase when the forecasting horizon expands. In this vein, multi-interval forecasting of traffic volumes requires a viable approach to conquer future uncertainties successfully. In this paper, a forecasting model is proposed which effectively addresses the uncertainties of future state based on the behaviors of temporal evolution of traffic volume states that intrinsically exits in the big past data. The model selects the past states from the big past data based on the state evolution of current traffic volumes, and then the selected past states are employed for estimating future states. The model was also designed to be suitable for data management systems in practice. Test results demonstrated that the model can effectively overcome the uncertainties over multiple time periods and can generate very reliable predictions in term of prediction accuracy. Hence, it is indicated that the model can be mounted and utilized on advanced data management systems.

• Journal of the Korea Computer Graphics Society
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• v.13 no.4
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• pp.7-12
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• 2007

With the advent of digital cinema, more and more movies are digitally produced, distributed via digital medium such as hard drives and network, and finally projected using a digital projector. However, digital cameras capable of shotting at 2K or higher resolution for digital cinema are still very expensive and bulky, which impedes rapid transition to digital production. As a low-cost solution for acquiring high resolution digital videos, we propose a hybrid camera consisting of a low-resolution CCD for capturing videos and a high-resolution CCD for capturing still images at regular intervals. From the output of the hybrid camera, we can synthesize high-resolution videos by software as follows: for each frame, 1. find pixel correspondences from the current frame to the previous and subsequent keyframes associated with high resolution still images, 2. synthesize a high-resolution image for the current frame by copying the image blocks associated with the corresponding pixels from the high-resolution keyframe images, and 3. complete the synthesis by filling holes in the synthesized image. This framework can be extended to making NPR video effects and capturing HDR videos.