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Isolation of CONSTANS as a TGA4/OBF4 Interacting Protein  

Song, Young Hun (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Song, Na Young (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Shin, Su Young (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Kim, Hye Jin (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Yun, Dae-Jin (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Lim, Chae Oh (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Lee, Sang Yeol (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Kang, Kyu Young (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Hong, Jong Chan (Environmental Biotechnology National Core Research Center, Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology Biotechnology Research Center, Gyeongsang National University)
Publication Information
Molecules and Cells / v.25, no.4, 2008 , pp. 559-565 More about this Journal
Abstract
Members of the TGA family of basic domain/leucine zipper transcription factors regulate defense genes through physical interaction with NON-EXPRESSOR OF PR1 (NPR1). Of the seven TGA family members, TGA4/octopine synthase (ocs)-element-binding factor 4 (OBF4) is the least understood. Here we present evidence for a novel function of OBF4 as a regulator of flowering. We identified CONSTANS (CO), a positive regulator of floral induction, as an OBF4-interacting protein, in a yeast two-hybrid library screen. OBF4 interacts with the B-box region of CO. The abundance of OBF4 mRNA cycles with a 24 h rhythm under both long-day (LD) and short-day (SD) conditions, with significantly higher levels during the night than during the day. Electrophoretic mobility shift assays revealed that OBF4 binds to the promoter of the FLOWERING LOCUS T (FT) gene, a direct target of CO. We also found that, like CO and FT, an OBF4:GUS construct was prominently expressed in the vascular tissues of leaf, indicating that OBF4 can regulate FT expression through the formation of a protein complex with CO. Taken together, our results suggest that OBF4 may act as a link between defense responses and flowering.
Keywords
Circadian Clock; CONSTANS; Defense Response; FT; OBF4; SA; Stress;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 10  (Related Records In Web of Science)
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