• 대한화장품학회지
• /
• 제44권2호
• /
• pp.191-200
• /
• 2018

본 연구에서는 제1형 프로콜라겐 분비량 측정을 통해 피부 주름개선 효과가 있는 천연물을 검색하던 중, 연꽃잎추출물(Nelumbo nucifera leaves extract, NLE)이 가장 우수한 효능을 나타냄을 확인하였다. 고압액체크로마토그래피로 NLE를 분석한 결과 isoquercitrin이 함유되어 있었으며, isoquercitrin도 normal human dermal fibroblast (NHDF)에서 제1형 프로콜라겐의 분비를 촉진한다는 것을 확인할 수 있었다. 또한 NLE와 isoquercitrin은 자유 라디컬 소거 활성을 가지고 있었으며, 특히 isoquercitrin은 인간 피부유래 세포주인 HaCaT 및 NHDF에서 활성산소종의 생성을 억제하고 총항산화능을 증가시켰다. 최종적으로 본 연구팀은 isoquercitrin의 함유량을 높이기 위해 정제한 NLE를 1.7% 함유한 크림(isoquercitrin 51 ppm 함유)을 제조하여 눈가의 주름개선 효과를 평가하였다. 30 ~ 65세의 한국인 여성 22명을 대상으로 얼굴의 한쪽 면엔 NLE 함유 크림을, 그리고 다른 한쪽 면엔 대조제품을 매일 2회씩, 8주간 연속 사용하도록 하였다. 그 결과 NLE 함유크림을 사용하였을 때 피부자극이 발견되지 않았으며, 대조제품과 비교하여 통계적 유의성 있게 눈가의 주름을 감소시키는 것을 확인할 수 있었다. 본 연구결과는 NLE 및 isoquercitrin이 우수한 콜라겐 생성 촉진 및 항산화 효능을 가지며, isoquercitrin 함유 NLE는 안전한 천연 주름개선 화장품 소재로 적용 가능하다는 것을 시사한다.

• BMB Reports
• /
• 제51권12호
• /
• pp.609-610
• /
• 2018

Glycosylation is one form of protein modification and plays a key role in protein stability, function, signaling regulation and even cancer. NleB and SseK are bacterial effector proteins and possess glycosyltransferase activity, even though they have different substrate preferences. NleB/SseKs transfer the GlcNAc sugar to an arginine residue of host proteins, leading to reduced $NF-{\kappa}B-dependent$ responses. By combining X-ray crystallography, NMR, molecular dynamics, enzyme kinetic assays and in vivo experiments, we demonstrated that a conserved HEN (His-Glu-Asn) motif in the active site plays a key role in enzyme catalysis and virulence. The lid-domain regulates the opening and closing of the active site and the HLH domain determines the substrate specificity. Our findings provide evidence for the enzymatic mechanism by which arginine can be glycosylated by SseK/NleB enzymes.

• Biomolecules & Therapeutics
• /
• 제20권1호
• /
• pp.96-103
• /
• 2012

In the present study, the inhibitory effect of neem leaf extract (NLE) on lipopolysaccaride (LPS)-induced nitric oxide (NO) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) production was examined both in vitro and in vivo. In vitro study revealed that NLE treatment ($100{\mu}g/ml$) inhibits LPS (100 ng/ml)-induced NO production by 96% and TNF-${\alpha}$ production by 32%. The reduction in NO production is probably conferred by the complete suppression of inducible nitric oxide synthase (iNOS) expression. Interestingly, in vivo NLE significantly improved the survival rate of mice in an experimental sepsis model. Administration of NLE (100 mg/kg) 24 h before LPS treatment (20 mg/kg) improved the survival rate of mice by 60%. The inhibition of plasma NO and TNF-${\alpha}$ production by NLE is likely to account for the improved survival of mice. Our results suggest that NLE may present a promising avenue in the development of therapeutic agents for the treatment of inflammatory diseases.

• 한국산업보건학회지
• /
• 제19권2호
• /
• pp.88-95
• /
• 2009

There are many assessment techniques used for occupational risk factors of MSDs in the workplaces. However, because all ergonomic assesment techniques or tools are based on theoretical background derived from workphysiology, biomechanics, psychophysics, industrial hygiene, work system, and etc, it is impossible to compare the assessment techniques. This study was conducted to compare the excess rates of risk factors among ergonomic assessment techniques and to make alternative methods. Site-visits to 6 automobile products and parts company provided data for process repeated work where the produced data was examined for evaluating the relationship between workplace lay-out and work posture by using ergonomic assessment techniques. We evaluated 157 jobs for simple repeated work and 37 jobs for manual materials handling (MMH). In simple repeated work, the exceeded rates of AC were 36.3% in OWAS method and 93.0% in RULA method. The exceeded rate for RULA method was significantly higher than those for OWAS method (p<0.05). In MMH, the exceeded rates of AC were 80.0% in NLE method and 76.5% in WAC method. Statistically significant differences were not identified in the exceeded rates for NLE and MAC methods (p<0.05). The analyzed results among ergonomic assessment techniques (OWAS, RULA, NLE/WAC) were applied to the same work places performing simple repeated work and manual materials handling simultaneously. The applied results showed statistically significant differences (p<0.05) among ergonomic assessment techniques (OWAS, RULA, NLE/WAC). Exceeded rates of four ergonomic assessment techniques in decreasing order was "RULA>NLE>WAC>OWAS". The RULA method was the strongest assessment technique for automobile products and parts company. We discovered that the results could easily be overestimated or underestimated when the ergonomic assessment techniques were not applied correctly during the evaluation process. Therefore, we recommend using at least 2 methods when evaluating and analysing the results.

• 생명과학회지
• /
• 제8권4호
• /
• pp.410-415
• /
• 1998

HPLC 및 electrospary mass spectrum으로부터 L-L dipeptide의 존제하에서 pepsin은 hexapeptide인 L-S-pNF-Nle-A-OMe를 가수분해하여 가수분해물외의 새로운 생성물을 합성하는 것이 확인되었다. 이 생성물은 254nm에서 p-nitro-Phe 잔기를 포함하는 peptide였다. 실험결과로부터 E(L-S-pNF)와 L-L 사이의 acyl transpeptidation에 의해 L-S-pNF-L-L가 생성됨을 뒷받침한다. 이러한 transpeptidation 결과는 product 저해실험에 의한 결과에 기초한 것과는 반대로 L-S-pNF가 해리되기전에 Nle-A-L-OMe가 먼저 한다는 것을 보여준다. 그리고, electrospray mass spectrum 으로부터 위에서 검출된 새로운 펩티드에 해당하는 peak (MW 636.1)을 얻었는데, 이는 새 펩티드의 생성을 확실히 증명하는 증거이다. 한편, Nle-A-L-OMe 생성에 대한 solvent isotope effect는 1.736$\pm$0.121이며 L-S-pNF는 2.28$\pm$0.184 그리고 L-S-pNF-L-L의 생성에는 inverse isotope effect로서 0.576$\pm$0.045였는데, 이는 상기 생성물 해리 순서를 확인시켜 준다. D$_{2}$에서 transpeptidation은 더 빠르기 때문에 isotopically-sensitive단계는 Nle-A-L-OMe해리후에 존재하는 것을 알 수 있다. 본 실험결과는, Rebholz and Northrop$^{1)}$ 및 Cho등의 $^{2)} iso-mechanism이론의 타당성을 제시한다.

• 한국산업보건학회지
• /
• 제15권2호
• /
• pp.104-113
• /
• 2005

A variety of ergonomic assessment methods of lifting tasks known as a major cause of work-related lower back pain have been used. But there is a limited information in choosing the most appropriate assessment method for a particular job and in finding out strengths and weakness of the methods. The purpose of this study was to assess and compare the ergonomic risks of lifting tasks in a marine diesel engine production industry by three lifting ergonomic assessment tools widely used: the National Institute for Occupational Safety and Health(NIOSH) Revised Lifting Equation(NLE), the Washington Administrative Code 296-62-0517(WAC), and the Snook Tables. Lifting index(weight of load/Recommended Weight Limit) of NLE($LI_{NLE}$) was above 1 at 34 tasks(75.6%) of a total number of 45 lifting tasks. LI of WAC($LI_{WAC}$) was above 1 at 11 tasks(24.4 %). LI of Snook Table($LI_{Snook}$) was above 1 at 29 tasks(64.4%). Thus, LI was high in orders of $LI_{NLE}$ > $LI_{Snook}$ > $LI_{WAC}$. There were significantly high correlations among three Lls(p<0.01). The correlation coefficients between $LI_{NLE}$and the other three Lls($LI_{WAC}$ and $LI_{Snook}$) were r=0.93 and r=0.88, respectively. The linear regression equations were y = 0.444x + 0.11(r=0.93) between $LI_{NLE}$ and $LI_{WAC}$, y = 0.93x + 0.008(r=0.88) between LI(NLE) and $LI_{Snook}$. The LI values by WAC was significantly lower than those by the other tools. The compared features, strength and limitation among these tools were described in this paper.

• 대한인간공학회지
• /
• 제25권2호
• /
• pp.43-50
• /
• 2006

The purpose of this study was to investigate applicability of NIOSH lifting equation(NLE) to analysis of workload for patient transferring. In principle, the NLE is not applied to analyzing workload of patient transferring, because 1) the task is generally performed by two or more persons; 2) unlike ordinary objects, human body of patients is basically unstable load with their location of the center of mass significantly varying during lifting activity; and 3) the task is done in a restricted work space. This study was conducted through comparison of NIOSH lifting indexes(LIs) and L5/S1 compressive forces by 3DSSPP for patient transferring tasks performed by 2~6 persons. The results showed that LIs are linearly correlated with L5/S1 compressive forces with correlation coefficient of 0.92, which resulted in a significant simple linear regression equation for LIs and L5/S1 compressive forces. Consequently, it was concluded that the NLE is applicable to transferring patient only with slight modification. Based on the results, instead of 1.0 originally used by NIOSH, the LI of 1.5 was proposed as a gauge to estimate whether or not the task needs corrective action to reduce risk for developing lifting-related low back pain.

• The Journal of Korean Physical Therapy
• /
• 제21권3호
• /
• pp.119-124
• /
• 2009

Purpose: The National Institute for Occupational Safety and Health (NIOSH) lifting equation (NLE) is a useful tool to ergonomically analyze a workload. The NLE has high reliability and it can assess tasks by analyzing the work process. The purpose of this case study was to try using the NLE to analyze the workload of transferring patients by physical therapists in the hospital setting. Methods: We observed a physical therapist (PT) transferring patients from a wheelchair to a tilt table and a therapeutic table in one day. Two types of patient transferring methods were evaluated; (1) the manual single person method of stand, pivot and transfer, and (2) manual two person lifting under the thigh and grasping the waist for totally dependent patients. Results: The NIOSH lifting indexes of a person grasping the waist during the manual two person lifting were 5.52~4.48 according to the patient's weight. The NIOSH lifting indexes were 3.34 and 4.48 for the tasks performed by the manual single person method. Conclusion: Because transferring patients is not done very frequently, patients transferring tasks by a PT are not included as one of the musculoskeletal disorder related risky work criteria of the Korea Ministry of Labor. But the NIOSH lifting indexes of a person grasping the waist during the manual two person lifting and the manual single person method were over the NIOSH recommended weight limit threshold.

• 한국인터넷방송통신학회논문지
• /
• 제17권4호
• /
• pp.163-172
• /
• 2017

최근 방송영상분야에서 영상편집 방법으로 비선형편집(Non-Linear Editing; NLE)을 주로 사용하고 있다. 기존의 선형편집에 비해 비선형편집은 컷(Cut)의 삽입과 삭제가 용이하고, 영상편집 시 원하는 위치의 영상에 바로 접근할 수 있다. 또한, 타이틀과 효과, 장면전환 효과를 적용할 수 있고 출력 전에 미리보기를 통해 적용한 타이틀과 효과를 확인하고 수정하는 것이 용이한 장점이 있다. 그러나, NLE편집을 처음 접하는 학생들이 그것을 배우는 것은 쉽지 않다. 본 논문에서는 비선형편집을 처음 접하는 학생들이 쉽게 배울 수 있는 기존의 상호동료교수법(Reciprocal Peer Tutoring; RPT)를 보완한 새로운 RPT 학습모형을 제시한다. 제안하는 교수학습모형을 적용한 실험집단과 적용하지 않는 비교집단으로 나누어 실험을 실시한다. 두 집단의 전체 평균, 성적 하위 집단의 학업 성취도, 표준편차, T검정과 함께 설문조사를 통한 만족도를 실시한다. 제안하는 학습모형을 적용한 실험집단이 통제집단에 비해 지표와 만족도에서 우월함을 보인다.

• Clinical and Experimental Pediatrics
• /
• 제48권3호
• /
• pp.342-345
• /
• 2005

저자들은 전신성 홍반성 루푸스 산모에서 출생한 생후 1개월된 신생아에서 매우 드물게 나타나는 항-$U_1RNP$ 항체 양성으로 인한 신생아 홍반성 루푸스 1례를 경험하였기에 그 임상적 특징을 문헌고찰과 함께 보고하는 바이다.