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Changes in Physicochemical Properties and Microbial Population during Fermenting Process of Organic Fertilizer (혼합발효 유기질비료의 발효과정 중 이화학성 및 미생물밀도 변화)

  • Lee, Jong-Tae;Lee, Chan-Jung;Kim, Hee-Dae
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.2
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    • pp.116-123
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    • 2004
  • This study was conducted to investigate the changes in physicochemical and microbiological properties during fermenting process of organic fertilizer which was made from the mixture of organic materials such as sesame oil cake, fish meal, blood meal, rice bran, ground bone meal, and natural minerals such as illite, crusted oyster shell and loess. They were mixed and fermented for 70 days. The sesame oil cake and rice bran, major ingredients for organic fertilizers, consisted of 7.6 and 2.6% total nitrogen, 3.6 and 4.6% $P_2O_5$, 1.4 and 2.2% $K_2O$, respectively. The ground bone meal included 29.2% $P_2O_5$ and illite included 3.8% $K_2O$. Temperature of organic fertilizer during the fermentation rapidly increased over $50^{\circ}C$ within 2 days after mixing and stabilized similar to outdoor temperature after 40 days. Moisture content decreased from 36.3 to 16.0% after 1 month. C/N ratio of organic fertilizer slightly increased until 30 days and thereafter, it slowly decreased, It resulted from the faster decrease of total nitrogen concentration compared with organic matter. Concentration of $NH_4-N$ in organic fertilizer rapidly increased from 1,504 to $5,530mg\;kg^{-1}$, the highest concentration after 10 days. Meantime, $NO_3-N$ concentration was low and constant about $150mg\;kg^{-1}$ over the whole fermenting period. This result seemed to be due to the high pH. The organic ferfilizer fermented for 70 days was composed of 2.7% N, 2.8% $P_2O_5$, 1.8% $K_2O$, and 35.9% organic matter. Total populations of aerobic bacteria, Bacillus sp. and actinomycetes, after fermenting process, were $12.5{\times}10^{10}$, $45.5{\times}10^{5}$ and $13.6{\times}10^{5}cfu\;g^{-1}$ respectively. Pseudomonas sp. was $71.9{\times}10^{7}cfu\;g^{-1}$ at first, but it rapidly decreased according to the rise of temperature. Yeasts played an important role in the early stage of fermentation and molds did in the late stage.

Evaluation of No-tillage Rice Cover Crop Cropping Systems for Organic Farming (벼 유기농업을 위한 무경운 피복작물 작부체계 평가)

  • Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.2
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    • pp.200-208
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    • 2010
  • The objectives of this study were to evaluate no-tillage cover crop cropping systems for organic farming in paddy. The experiment was performed at Ihyeon series (silt loam: 9.1% sand, 73.0% silt and 17.9% clay) which affected by different management practices. Planthopper population per 20 plant was significantly higher 65.3 for conventional tillage with chemical compounds (control) compared with 3.4~9.6 for no-tillage treatments (without rice straw or green manure, amended with rice straw, hairy vetch, rape, rye, and Chinese milk vetch). Also, disease severity of sheath blight was significantly higher 10.5% for control compared to 0.7~2.9% for no-tillage treatments. Four weed species, namely Monochoria vaginalis, Ludwigia prostrata, Rotala indica, and Aneilema keisak occurred in no-tillage paddy, whereas Monochoria vaginalis occurred in control only. The pH, available phosphate, and microbial biomass C in paddy were steeply decreased in response to submerging, but increased at first heading stage. Soil $NH_4$-N content at first heading stage was significantly higher in no-tillage treatments compared with control. The grain yield was significantly higher in 4.30 Mg $ha^{-1}$ for control than other treatments. Meanwhile, rice productivity was significantly higher in 2.69 Mg $ha^{-1}$ for no-tillage amended with Chinese milk vetch compared to other no-tillage treatments. The number of panicle per plant, grain number perpanicle, and percent ripened grain were highly related for increasing the yield of rice. These results show that Chinese milk vetch was optimum cover crop for organic farming in no-tillage paddy.

Synecological Study of the Naturalized Plant Communities in Old-Andong City (구 안동시역에 분포하는 귀화식물군락의 생태학적 연구)

  • 송종석;안상흥
    • The Korean Journal of Ecology
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    • v.22 no.3
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    • pp.169-179
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    • 1999
  • The present study was undertaken to classify and describe the spring naturalized plant communities in old-Andong city by the methodology of the ZM school of phytosociology. As a result, the vegetation was classified into the six communities and two subcommunities: A. Bromus tectorum community A-a. Bromus japonicus subcommunity, A-b. Melica onoei subcommunity; B. Poa pratensis community; C. Rumex crispus community; D. Oenothera lamarckiana community; E. Ambrosia artemisiifolia var. eiatior community; F. Rumex conglomeratus community. The total taxa of the naturalized plant communities consisted of 31 families, 86 genera, 114 species and 13 varieties. Of which the taxa of the naturalized plant species consisted of 8 families, 21 genera, 25 species and 1 variety. The result of Bray-Curtis ordination revealed that the plots suveyed were arranged according largely to the vegetation units of' the communities. Also the interspecific affinity was examined by an analysis of interspecific association and the main component species in the communities were divided into two groups. The naturalized rate was higher in the communitise affected by strong human impacts, while was lower in the communities affected relatively less by human impacts. On the other hand the seasonal changes of the communities and the naturalized rate between the spring and the autumn were investigated. Many naturalized communities present in Spring were replaced by the other native ruderal communitues in the Autumn. The naturalized rate based on the dominance was largely decreased over from the spring to the Autumn.

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Effect of Various Forms of Floor System on Performance of Meat-type Duck and Environments of Duck House (오리사 바닥 형태가 육용오리의 생산성 및 사육환경에 미치는 영향)

  • Bang, Han-Tae;Kim, Dong-Woon;Hwangbo, Jong;Na, Jae-Cheon;Kang, Hwan-Ku;Kim, Min-Ji;Mushtaq, M.M.H.;Parvin, R.;Choi, Hee-Chul;Lee, Sang-Bae;Kang, Min;Kim, Ji-Hyuk
    • Korean Journal of Poultry Science
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    • v.40 no.3
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    • pp.253-262
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    • 2013
  • This experiment was conducted to investigate the effects of floor type and heating system on performance, housing environment and health status of ducks reared in three types of duck house (OD : Open floor house-Direct heating system, OF : Open floor house-Floor heating system and LD : Loft type house-Direct heating system). In OF treatment, PVC pipes were installed for heating under concrete floor and covered with litter. In LD treatment, plastic mesh was installed 50 cm above the floor so that duck's droppings can pass through it. Each treatment had four replicates of 25 birds (Cherry Valley duck breed) per pen. There were no significant differences in weight gain and feed intake of ducks for 6 weeks among all treatments. However, feed conversion ratio in LD was significantly higher (p<0.05) than that in OF. No differences were found in carcass charac- teristics, with the exception of abdominal fat weight where OF were higher than the others. Concentrations of $CO_2$ and $NH_3$ gas in OD were higher than those of OF and LD at 3, 4 and 5 weeks. Moisture content in litter of OF was lower than that of OD. In contrast, the amount of dust in the air was higher in OF than in OD. The amount of fuel used for 6 weeks in LD was lower about 21% than that in OD. Some of unusual symptoms were observed in open floor house and loft type house, such as lying, spraddle legged, twisted ankle and legs, wounded sole, or etc. No components of leukocyte and erythrocyte of blood were significantly different among all treatments. The results of this experiment showed that OF and LD systems had no positive effects on performance of meat type commercial duck. However, there were some positive effects of certain house type for the improvement of environmental condition in duck house for hygienic production. In the future, more research on the effect of various facilities and systems for duck house is needed.

Study on Causes and Countermeasures for the Mass Death of Fish in Reservoirs in Andong-si (안동시 저수지에서의 대량 어류 폐사에 대한 원인과 대책에 관한 연구)

  • Su Ho Bae;Sun Jin Hwang;Youn Jung Kim;Cheol Ho Jeong;Seong Yun Kim;Keon Sang Ryoo
    • Korean Journal of Environmental Agriculture
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    • v.42 no.1
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    • pp.52-62
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    • 2023
  • This study focused on determining the specific causes and prevention methods of mass fish deaths occurred in five reservoirs (Gagugi, Neupgokgi, Danggokgi, Sagokji, and Hangokji) in Andong-si. For this purpose, a survey of agricultural land and livestock in the upper part of the reservoirs and analysis of water quality in the reservoir irrespective of whether it rains or not were conducted. We attempted to examine the changes in dissolved oxygen (DO) in the surface and bottom layers of reservoirs and changes in DO depending on the amount of livestock compost and time. Based on the above investigations, treatment plans were established to efficiently control the inflow of contaminated water into reservoirs. The rainfall and farmland areas in the upper part of the reservoir were investigated using Google and aviation data provided by the Ministry of Land, Infrastructure, and Transport. The current status of livestock farms distributed around the reservoirs was also examined because compost from these farms can flow into the reservoir when it rains. Various water quality parameters, such as phosphate phosphorus (PO4-P) and ammonium nitrogen (NH3-N), were analyzed and compared for each reservoir during the rainy season. Changes in the DO concentration and electrical conductivity (EC) were also observed at the inlet of the reservoir during raining using an automated instrument. In addition, DO was measured until the concentration reached 0 ppm in 10 min by adding livestock compost at various concentrations (0.05%, 0.1%, 0.3%, and 0.5% by wt.), where the concentration of the livestock compost represents the relative weight of rainwater. The DO concentration in the surface layer of reservoirs was 3.7 to 5.3 ppm, which is sufficient for fish survival. However, the fish could not survive at the bottom layer with DO concentration of 0.0-2.1 ppm. When the livestock compost was 0.3%, DO required 10-19 h to reach 0 ppm. Considering these results, it was confirmed that the DO in the bottom layer of the reservoir could easily change to an anaerobic state within 24 h when the livestock compost in the rainwater exceeds 0.3%. The results show that the direct cause of fish mortality is the inflow of excessive livestock compost into reservoirs during the first rainfall in spring. All the surveyed reservoirs had relatively good topographical features for the inflow of compost generated from livestock farms. This keeps the bottom layer of the reservoir free of oxygen. Therefore, to prevent fish death due to insufficient DO in the reservoir, measures should be undertaken to limit the amount of livestock compost flowing into the reservoir within 0.3%, which has been experimentally determined. As a basic countermeasure, minerals such as limestone, dolomite, and magnesia containing calcium and magnesium should be added to the compost of livestock farms around the reservoir. These minerals have excellent pollutant removal capabilities when sprayed onto the compost. In addition, measures should be taken to prevent fish death according to the characteristics of each reservoir.

Distribution of Nutrients in Dae-Cheong Reservoir Sediment

  • Hwang Jong Yeon;Han Eui Jung;Kim Tae Kehn;Kim Shin Jo;Yu Soon Ju;Yoon Young Sam;Jung Yong Soon;Park Pan Wook
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.2 no.2
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    • pp.169-179
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    • 1998
  • This paper was performed to estimate interrelations between humus level of sediments and nutrient release from sediments in Dae-cheong reservoir. For investigations, sediments were sampled in June and October, in 1997 at fish farms, embayment, and the main stream of Dae-cheong reservoir. Items for investigation are as follows; water content, weight loss on ignition(IG), porosities of sediments, contents of element such as hydrogen, nitrogen, carbon, and nutrient release rates. Water contents and porosities were measured to conjecture the physical trait and grain size trait. Weight loss on ignition was measured to determine the contents of organic substance. For determination of the humus level of sediments, carbon and nitrogen contents were measured by elemental analyzer. As a result of elemental analysis, C/N ratio was determined in the range of $3.0\~13.1$. From the elemental analysis, humus level of Dae-cheong reservoir sediment was estimated from mesohumic state to oligotrophic state. For the determination of nutrient release rate, $PO_4-P$ and $NH_4-N$ concentrations of interstitial water and overlying water were measured. By using the concentration difference between interstitial water and overlying water and using the Fick's diffusion law, the release rates of phosphorus and nitrogen from the sediment samples were calculated. Release rates of nutrients which directly influence to the water quality were $0.05\~8.63mgP/m^2day$ and $4.99\~36.56mgP/m^2day$. It was found that release rate was measured higher in the 1st sampling period than in the 2nd sampling period. For the determination of phosphorus content in sediment, TPs were measured in 807\~1542{\mu}g/g$ in the 1st samling period and $677\~5238{\mu}g/g$ in the End samling period. Phosphorus release rate and phosphorus content were not interrelated each other.

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Influence of Percolation Rate on Nutrient Uptake and Yield of Paddy Rice (투수속도(透水速度)가 수도(水稻)의 양분흡수(養分吸收) 및 수량(收量)에 미치는 영향(影響))

  • Shin, Weon-Kyo
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.3
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    • pp.218-223
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    • 1984
  • In order to analyze the effects of percolation rate on the growth and yield of rice plants in paddy soils, pot (1a/2000) experiments were carried out. The soil used was highly concentrated with various salts in the horticulture area under vinyl house cultivation, and Samgang variety of Japanica rice was planted. With the increasing rates of percolation, $SiO_2$ and $Ca^{2+}$ were more leached out from soils than supplied by irrigation while $K^+$ and $NH_4{^+}$ were more supplied to soils than leached out. The root activity in the late growth stage was higher in the percolation pots than in the non-percolation pots. Amounts of nutrient uptake of T-N, $P_2O_5$, $K_2O$ and MgO were increased gradually with the increase of percolation rate, but that of $SiO_2$ was maximum at 10mm per day. The percolation rate of 5~10mm per day was considered to be the optimum condition for obtaining more than 95% of relative yield in rice cultivation.

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Responses of Dairy Cows to Supplemental Highly Digestible Rumen Undegradable Protein and Rumen-protected Forms of Methionine

  • Sun, T.;Yu, X.;Li, S.L.;Dong, Y.X.;Zhang, H.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.5
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    • pp.659-666
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    • 2009
  • Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 193${\pm}$13 days in milk were used in a 4${\times}$4 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation ($NE_L$), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, $NH_3g-N$ and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.

Isolation and Characterization of Immunomodulatory Glycoprotein from the Root of Panax ginseng

  • Shin, Han-Jae;Park, Kyeong-Mee;Kim, Young-Sook;Nam, Ki-Yeul;Lee, You-Hui;Park, Jong-Dae
    • Journal of Ginseng Research
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    • v.24 no.3
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    • pp.128-133
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    • 2000
  • A high molecular (more than 10 kDa) fraction, showing mitogenic and comitogenic activities in spleen cells of mouse, was isolated from water extract of ginseng. The crude protein substance prepared by 80% (NH$_4$)$_2$SO$_4$ precipitation from this fraction was purified and isolated by DEAE Sepharose column chromatography. Among the fractions eluted, it was found that four kinds of fractions eluted with 0 to 1 M NaCl gradient were glycoproteins, which induced proliferation of spleen cells and increased NO production in macrophages. Among them, F-2 fraction, which contained 35.9% protein,49.4% neutral sugar and 12.5% uronic acid, was found to show mitogenic activity as strong as that of LPS (lipopolysaccharide) at a concentration of 100 $\mu\textrm{g}$/ml and to remarkably stimulate NO production by murine macrophages at a concentration of 500 $\mu\textrm{g}$/ml. When F-2 is deproteinized, the mitogenic activity of F-2 was decreased significantly to 70.9% as compared with that of F-2. This results suggests that the protein moiety of F-2 may play an important role in immunomodulating activity of glycoprotein from the root of Panax ginseng.

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Electrochemical Reduction for trans-Complexes of Cobalt (III) with Bis(ethylenediamine) and Monodendate Ligands (한자리 리간드를 포함하는 트란스비스 (에틸렌디아민) 코발트 (III) 이온의 전극 환원반응)

  • Jung-Ui Hwang;Jong-Jae Chung;Jae-Duck Lee
    • Journal of the Korean Chemical Society
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    • v.33 no.2
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    • pp.215-224
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    • 1989
  • Electrochemical reductions of $trans-[Co(en)_2X_2](ClO_4)_n$ (where X is cyanide, nitrite, ammonia, and isothiocyanate) were investigated by cyclic voltammetry and polarography at mercury and glassy carbon electrode. $trans-[Co(en)_2(CN)_2]ClO_4$ was reduced to Co(II) complex followed by adsorption to the mercury electrode. Cyanide ion was not released from the reduced Co(II) complex but the cyanide and (en) were released after the reduction to metallic cobalt. The other complexes except $trans-[Co(en)_2(CN)_2]ClO_4$ were reduced to cobalt(II) complexes followed by release of monodendate ligand, and (en) was released at the reduction step to metallic cobalt. $trans-[Co(en)_2(NO_2)_2]ClO_4$ was reduced to cobalt(Ⅱ) complex, and $NO_2^-$ ion was released followed by electroreduction through ECE mechanism at pH 2. On glassy carbon electrode, all complexes of Co(III) were reduced to Co(II) complexes with irreversible one-electron diffusion controlled reaction in which (en) was not released at this step. Increasing absorption wave number of complexes caused to negative shift of peak potential.

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