• 한국식품영양학회지
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• 제33권2호
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• pp.142-148
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• 2020

The purpose of this study was to investigate the prevalence, toxin gene profiles, and enterotoxin producing ability of Bacillus cereus isolated from environment-friendly vegetables and good agricultural practices (GAP) vegetables. A total of 49 vegetables including 40 environment-friendly vegetables and 9 GAP vegetables were tested. The Vitek 2 system was used to identify B. cereus and the PCR was used to detect 6 toxin genes, respectively. B. cereus was detected in 34 (69.3%) of 49 vegetables and the prevalence of B. cereus in GAP vegetables (44.4%) was lower than in the environment-friendly vegetables (75.0%). The detection rates of entFM, nheA, hblC, and cytK enterotoxin genes, respectively, among all isolates were 100%, 97.0%, 88.2%, and 73.5%, respectively. All of the isolates had at least one or more enterotoxin gene and 20 isolates (58.8%) had hemolysin BL enterotoxin producing ability. The risk of food poisoning from the environment-friendly vegetables and the GAP vegetables has been shown as constant. Thus, it is necessary to expand the supply of GAP vegetables showing lower B. cereus contamination than the environment-friendly vegetables. The characteristics of the environment-friendly vegetables and the GAP vegetables that must be consumed after cleaning should be disseminated to consumers regarding food poisoning prevention.

• 동굴
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• 제74호
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• pp.23-31
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• 2006

(1) Kaeusetgul Cave in Kimnyong-Ri, Jeju-Do, Korea. Kaeuset-gul Cave (KC) is situated in NNE area of the Manjang-gul cave (125m a.s.l.). Kaeuset-gul Cave lies at $126^{\circ}45'22"$ E in longitude and $33^{\circ}33'09"$ N in latitude. The coast belong Kimnyeong-Ri, Kujwa-eup, Jeju-Do. Altitude of the cave-entrance is 10m and length of the cave is 90m. Lava hand-specimens of KC are studied by X-ray fluorescence analysis (XRF). Average major chemical components of specimens from KC is as follows (wt.%); $SiO_2=47.03$, $TiO_2=3.16$, $Al_2O_3=18.41$, FeO*=13.53, MnO=0.14, MgO=5.05, CaO=8.66, $Na_2O=2.81$, $K_2O=0.67$, $P_2O_5=0.55$ in KC. Polarizing microscopic studyindicates that these specimens are described of alkali-basalt. (2) Tachibori Fuketsu (Cave) in Shizuoka Prefecture, Fuji Volcano, Japan Tachibori Fuketsu lies attoward the south in skirt of the Fuji volcano, $138^{\circ}42'04"$ east longitude and $35^{\circ}18'00"$ north latitude. The location of cave entrance is 2745, Awakura, Fujinomiya-shi, Shizuoka Prefecture. The above sea level and length of Tachibori Fuketsu are 1,170m and 82m. Average major chemical components of specimens from cave areas follows (Total 100 wt.%) ; ($SiO_2$=50.52, $TiO_2$=1.69, $Al_2O_3$=15.47, FeO*=13.13, MnO=0.20, MgO=5.97, CaO=9.17, $Na_2O$=2.52, $K_2O$=0.94 and $P_2O_5=0.40).$ Polarizing microscopic study indicates that these specimens may belong to tholeiite-basalt series. According to polarizing microscopic study, Au (Augite), P1 (Plagioclase), and O1 (Olivine) are contained as phenocryst minerals. (3) Gorely Cave in Kamchatka Peninsula, Russia Gorely caldera is located at the southeastern part of Kamchatka Peninsula, about 75km southwest of Petropavlovsk-Kamchatskiy.. Gorely lava caves are situated in NHE area of Mt. Gorely volcano (1829m a.s.1.). One of lava cave (Go-9612=K-1) lies at $158^{\circ}00'22"$ east longitude and $52^{\circ}36'18"$ north latitude. The elevation of cave entrance is about 990m a.s.1. and the main cave extends in the NNW direction for about 50m by 15m wide and 5m in depth. The cave of K-3is near the K-1 cave. "@Lava hand-specimens K-1 and K-3 caves are studied by X-ray fluorescence analysis and polarizing microscopic observation. Average major chemical components of specimens from these caves are as follows (wt.%) ;($SiO_2$=55.12, $TiO_2$=1.25, $Al_2O_3$=16.07, T-FeO* =9.41, MnO=0.16, MgO=5.01, CaO=7.21, $Na_2O$=3.39, $K_2O$=1.92, $P_2O_5$=0.45) and these values indicate that the Gorely basaltic andesite belong to high alumina basalt. Polarizing microscopic study indicates that these specimens are described of Augite andesite.

• 한국식품위생안전성학회지
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• 제27권1호
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• pp.30-36
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• 2012

어린이집 유아 손의 미생물 오염도를 분석하고 손 씻기 교육의 미생물 저감화 효과를 실험하여 어린이집 유아 손의 위생 안전성을 확보하고자 어린이집 유아 59명을 대상으로 실험하였다. 일반세균수의 경우 평균 $3.72{\pm}0.38log\;CFU/hand$로 검출되었고, 위생지표미생물인 대장균군의 경우 남자어린이 18명 중 2명(11.1%)과 여자어린이 17명 중 3명(17.6%), 총 35명 중 5명(14.3%)에서 검출되어 어린이들에 대한 반복적인 손 씻기 지도가 필요한 것으로 판단되었다. $Staph.$ $aureus$는 35명 중 9명(25.7%), $B.$ $cereus$는 35명 중 16명(45.7%)에서 검출되었고 남자어린이의 손이 식중독 미생물 오염에 가장 취약한 것으로 나타났다. $Staph.$ $aureus$ 장독소는 9균주 중 $Staph.$ $aureus$ Hand-1 한 균주에서만 SEA 장독소가 검출되었으나 HBL과 NHE 장독소 중 한 가지 이상의 장독소를 생산하는 $B.$ $cereus$는 총 12균주(70.0%)로 나타나 어린이 손에 의한 교차오염 위험성이 상존한다고 판단되었다. 손 씻기 교육의 미생물 저감화 효과를 실험한 결과 교육을 실시한 그룹이 0.18 log CFU/hand 일반세균수가 더 저감화가 되었으나 저감화 정도가 매우 미약하여 손에 부착되어 있는 세균을 완전히 제거하기 위해서는 손 씻기와 손 소독을 병행하며 지속적인 손 씻기 교육을 실시하여야할 것으로 판단되었다.