• Proceedings of the Korea Contents Association Conference
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• 2019.05a
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• pp.469-470
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• 2019

차세대염기서열분석법(NGS, Next Generation Sequencing) 기술은 하나의 유전체를 무수히 많은 조각으로 분해하여 각 조각을 동시에 읽어낸 뒤, 전산기술을 이용하여 조합함으로써 방대한 유전체 정보를 빠르게 해독하는 방법이다. 한편, 액체 생검(LB, liquid biopsy)이란 암세포가 깨지면서 생기는 미량의 DNA 조각을 말초혈액 속에서 찾아내 암을 진단하는 기술로 조직 생검(tissue biopsy)에 비해 비침습적이다. 본 논문은 NGS와 LB 기술을 접목했을 때 확진이 가능하고 예후 및 치료경과의 예측이 가능함을 제언하였다.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.1
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• pp.48-59
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• 2009

Next generation mobile communication system will have various access networks and provide seamless multimedia data service to mobile nodes. The WiNGS system, one of these access network, has superior RAT(Radio Access Technology) and network connectivity other than previous ones. In this paper, we propose link-layer SR-ARQ between mobile node and WAGW to solve packet re-ordering problem during handover. In the proposed scheme, we apply the adaptive timer at the SR-ARQ sender to reduce unnecessary packet retransmission during handoff and delayed NAK algorithm at the SR-ARQ receiver for delaying NAK response due to temporary out-of-sequenced frames. We evaluate the performance of the proposed scheme by implementing the new SR-ARQ protocol at the link-layer using ns-2 simulator, and show the handover performance is improved greatly by preventing unnecessary retransmission.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.1
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• pp.16-28
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• 2023

Lung adenocarcinoma accounts for about 40% of all lung cancers. With the recent development of gene profiling technology, studies on mutations in oncogenes and tumor suppressor genes, which are important for the development and growth of tumors, have been actively conducted. Companion diagnosis using next-generation sequencing helps improve survival with targeted therapy. In this study, formalin-fixed paraffin-embedded tissues of non-small cell lung cancer patients were subjected to hematoxylin and eosin staining for detecting genetic mutations that induce lung adenocarcinoma in Koreans. Immunohistochemical staining was also performed to accurately classify lung adenocarcinoma tissues. Based on the results, next-generation sequencing was applied to analyze the types and patterns of genetic mutations, and the association with smoking was established as the most representative cause of lung cancer. Results of next-generation sequencing analysis confirmed the single nucleotide variations, copy number variations, and gene rearrangements. In order to validate the reliability of next-generation sequencing, we additionally performed the existing genetic testing methods (polymerase chain reaction-epidermal growth factor receptor, immunohistochemistry-anaplastic lymphoma kinase (D5F3), and fluorescence in situ hybridiation-receptor tyrosine kinase 1 tests) to confirm the concordance rates with the next-generation sequencing test results. This study demonstrates that next-generation sequencing of lung adenocarcinoma patients simultaneously identifies mutation.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2008.10a
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• pp.455-459
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• 2008

This study defines the Next Generation System(NGS) built by domestic financial businesses and classifies their architecture into two typical types according to the duration of the project and the relative cost of IT investment in the short term: the Big Bang approach and the Phase approach. Herein, we study the two approaches as alternatives in developing the Next Generation System, and derive the factors that are to be considered in the evaluation of the two alternatives for financial businesses. The set of standards for the choice between the two models are grouped into categories that constitute performance evaluation for IT - Cost, Performance and Risk. We drill down further into each category to second and third subordinate levels to derive detailed selection criteria. Based on the criteria drawn from the study, we conduct a survey with information system planners, IT managers and specialists at financial companies who are currently planning, developing or have completed a Next Generation System. Survey results are analyzed using the AHP methodology to compare and understand the different approach in the implementation of NGS for financial business.

• Journal of Life Science
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• v.30 no.1
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• pp.64-69
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• 2020

The European honeybee (Apis mellifera L.) has multiple anti-microbial peptides, but many were unknown and demands for their characterization have increased. This study therefore focused on identifying novel anti-microbial peptides (AMPs) from A. mellifera L. To obtain high-throughput transcriptome data of the honeybee, we implemented next-generation sequencing (NGS), isolating novel AMPs from total RNA, and generated 15,314 peptide sequences, including 44 known, using Illumina HiSeq 2500 technology. The uncharacterized peptides were identified based on specific features of possible AMPs predicted in the sequencing analysis. AMP5, one such uncharacterized peptide, was expressed in the epidermis, body fat, and venom gland of the honeybee. We chemically synthesized this peptide and tested its anti-bacterial activity against Gram-negative Escherichia coli (KACC 10005) and Gram-positive Bacillus thuringiensis (KACC 10168) by anti-microbial assay. AMP5 exhibited anti-bacterial activity against E. coli (MIC50=22.04±0.66 μM) but not against B. thuringiensis. When worker bees were injected with E. coli, AMP5 was up-regulated in the body fat. This study therefore identified AMP5 in adult European honeybees and confirmed its anti-bacterial activity against Gram-negative E. coli.

• Journal of the Korean Society for Environmental Technology
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• v.19 no.6
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• pp.550-555
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• 2018

International Maritime Organization (IMO) is one of the most effective organizations in evolving international law for the protection and conservation of the marine environment. The IMO, MARPOL(Marine Pollution) 73/78 contains six Annexes that provide an overarching framework for the objectives of the international marine pollution. Annex IV was regulated by 64 th resolution in 2012 to control sea pollution from sewage. In 2014 large-scale wastewater treatment and nutrient removal device was developed with a grant from the Ministry of Oceans and Fisheries. A combined new process of Sequence Batch Reactor (SBR) and Membrane Bioreactor(MBR) was developed to overcome the pollution caused by shipboard sewage. In the present study, shipboard sewage wastewater was treated by mixing and aeration cycle in the newly developed SBR process. Furthermore, during analysis by NGS technique(Macrogen Co., Ltd.), dominant species of bacteria were found in the aeration tank of the Bench-scale wastewater treatment facility. Bacteroidetes and Gammaproteobacteria accounted for 27.1 % of the aerobicbacteria and 16.8 % of the anaerobicbacteria, respectively. Microorganisms play a vital role in shipboard wastewater treatment. A further detailed study is required to understand the precise role of the microorganisms in the wastewater treatment.

• Proceedings of the Korea Contents Association Conference
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• 2019.05a
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• pp.9-10
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• 2019

NGS 분석과정 중 증폭된 DNA 리드(Read)는 중복이 발생하여 이로 인해 다음 단계의 정확도 저하 등의 문제를 야기한다. 이 논문에서는 기존 DNA 리드 중복 제거 도구 중 하나인 Samblaster의 중복제거 알고리즘을 이용하여 분산 병렬 형태로 중복제거를 수행하는 방법을 제안한다. 제안하는 중복제거 방법은 중복제거 전단계인 정렬단계애서 정렬된 리드에 대해 중복제거 키를 생성하고 이를 스트림으로 전달하여 중복제거를 수행한다.

• Annual Conference of KIPS
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• 2021.11a
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• pp.32-35
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• 2021

단일 가상화 서비스 플랫폼은 메모리 및 컴퓨팅 집약적 워크로드를 수행하기 위한 고성능 시스템 환경의 신속한 구축을 지원하는 클라우드 기반의 소프트웨어 정의 서버를 위한 핵심 기술이다. 본 연구는 다수의 물리 노드를 통합하여 하나의 고성능 단일가상서버로 구성하기 위해 개발된 HCC 단일 가상화 서비스 플랫폼에서 대용량 데이터 처리 및 대규모 연산이 필요한 NGS 기반 농생명유전체 조립 프로그램과 이상 기상의 탐지 분석을 위한 GOES 위성자료 전처리 프로그램을 시험하여 활용 적합성을 검증하였다.

• Annual Conference of KIPS
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• 2017.04a
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• pp.848-849
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• 2017

차세대 염기 분석(NGS) 장비의 발달로 시퀀스 분석에 대한 연구는 가속화 되고 있다. 조각들로 이루어진 리드들을 어셈블하는 방법부터 이미 유전체의 정보가 알려진 데이터베이스를 이용하여 정보를 명명하는 방법까지 다양한 방법들에 대한 방법들이 주를 이루고 있다. 하지만 어셈블하는 툴마다 다른 입력 포맷을 요구하고 있어 NGS의 결과로 다양한 방법으로 어셈블하여 비교 분석하기 쉽지 않다. 뿐만 아니라 생물 학자들이 세포내의 진화나 계통발생학적 분류를 위한 연구를 위해서 유전체 지도 완성 후 세포내의 기관별 분리 분석이 필요하나 참고 시퀀스로부터 매핑 및 기관별 분리 분석을 위해 사용목적에 따라 입력 포맷이 다른 다른 툴을 사용해야한다. 따라서 본 논문에서는 핵, 색소체, 미토콘드리아와 같은 세포내 기관에 대한 정보를 알기 위해 최소의 정보를 입력하여 분석하고자하는 시퀀스을 입력하여, 최대한 유사하게 매칭되는 유전체를 찾아 분석하는 방법을 제안함으로써, 진핵세포내의 발생학적 연구에 도움이 되는 방법을 제안하고자 한다.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.473-482
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• 2013

Molecular markers, as an efficient selection tool, have been and is being used for practical breeding program in chili pepper (Capsicum annuum L.). Recently, a lot of researches on inheritance and genetic analysis for quantitative traits including capsaicinoids, carotenoids, and sugar content in pepper are being performed worldwide. It has been also reported that QTL mapping is a necessary tool to develop molecular markers associated with the quantitative traits. In this study, we suggested a new method to construct a pepper genetic map using SNP (HRM) markers generated from NGS resequencing of female and male parents. Plant materials were C. annuum 'NB1' (female parent), C. chinense 'Jolokia' (male parent), and their $F_2$ population consisting of 94 progenies. Sequences of 4.6 Gbp and 6.2 Gbp were obtained from NGS resequencing of 'NB1' and 'Jolokia', respectively. Totally, 4.29 million SNPs between 'NB1' and 'Jolokia' were detected and the 1.76 million SNPs were clearly identified. Among them, total 145 SNP (HRM) primer pairs covering pepper genetic map were selected, and the 116 SNP (HRM) markers of them were located on this map. Total distance of the map, which consisted of 12 linkage groups and matched with basic chromosome numbers of pepper, was 1,167.9 cM. According to the mapping result, we concluded that our mapping method was suitable to construct a pepper genetic map fast and accurately. In addition, the genetic map could be directly used for QTL analysis of traits different between both parents.