• 한국균학회지
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• 제49권3호
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• pp.405-412
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• 2021

복령은 한국, 일본, 중국을 포함한 동북아시아 지역에서 사용되어 왔던 중요한 약용버섯이다. 복령은내부색깔을기준으로적복령과백복령으로구분하며, 일반적으로 백복령이 농가에서 재배되고 있다. 본 연구에서는 2가지 재배 복령과 3가지 야생 복령을 이용하여 이원교배를 통해 만들어진 균주로부터 톱밥배지에서 균핵을 형성하는 균주를 선발하려고 하였다. 단포자는 PDA 배지에서 만들어진 복령의 자실체로부터 분리하였다. 분리된 교잡균주 338개 중 39개가 소나무 톱밥배지에서 3개월 동안 배양하는 과정에서 백색 또는 황색의 균핵을 형성하였다. 톱밥배지를 이용한 균핵 형성 균주의 선발은 매우 간단하고 쉬운 방법이며 본 논문에서 처음으로 제시하는 것이다. 그리고 선발된 균주는 향후 새로운 품종을 개발하고자 야외실험을 시도할 계획이다.

• 한국수정란이식학회지
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• 제33권4호
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• pp.195-203
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• 2018

Interferon-tau (IFNT) is known as a major conceptus protein that signals the process of maternal recognition of pregnancy in ruminants. Also, multiple interferon genes exist in cattle, However, molecular mechanisms of these bovine IFNT (bIFNT) genes whose expressions are limited have not been characterized. We and others have observed that expression levels of bovine subtype IFNT genes in the tissues of ruminants; thus, bIFNT1 and other new type I (bIFNTc1/c2/c3) gene co-exist during the early stages of conceptus development and non-trophoblast cells. Its genes transcription could be regulated through CDX2 and ETS2 and JUN and/or cAMP-response element binding protein (CREB)-binding protein (CREBBP) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. Bovine ear-derived fibroblast cells, were co-transfected with luciferase reporter constructs carrying upstream (positions -1000 to +51) regions of bIFNT1 and other new type I gene and various transcription factor expression plasmids. Compared to each - 1kb-bIFNT1/c1/c2/c3-Luc increased when this constructs were co-transfected with CDX2, ETS2, JUN and/or CREBBP. Also, Its genes was had very effect on activity by CDX2, either alone or with the other transcription factors, markedly increased luciferase activity. However, the degree of transcriptional activation of the bIFNTc1 gene was not similar to that bIFNT1/c2/c3 gene by expression plasmid. Furthermore, Sequence analyses also revealed that the expression levels of bIFNT1/c2/c3 gene mRNAs expression were highest on day 17, 20 and 22 trophoblast and, Madin-Darby bovine kidney (MDBK), Bovine ear-derived fibroblast (EF), and endometrium (Endo) non-trophoblast cells. But, bIFNTc1 mRNA had not same expression level, bIFNTc1 lowest levels than those of IFNT1/c2/c3 gene in both trophoblast and non-trophoblast cells. These results demonstrate that bovine subtype bIFNT genes display differential, in the trophoblast and non-trophoblast cells.

• 원예과학기술지
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• 제34권6호
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• pp.959-965
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• 2016

껍질째 먹는 배 '조이스킨'은 1994년 국립원예특작과학원 배연구소에서 '황금배'와 '조생적'을 교배하였다. 317개 교배실생 중 식미가 우수하고 껍질을 벗기지 않고 먹을 수 있는 '17-04-53'을 2006년 최초로 선발하고 2006년부터 2011년까지 9개 지역, 10개 장소에서 지역적응 시험을 거친 후, 최종 선발하여 '조이스킨'으로 명명하였다. '조이스킨'은 '황금배'와 마찬가지로 수세가 강하고, 반개장형이다. '조이스킨'의 평균 만개기는 4월 21일로 '황금배'와 유사하며 숙기는 9월 6-8일로 '황금배' 보다 7-8일 이상 빠르고 과형은 원형, 성숙기에는 선황색을 띈다. 평균과중은 320 g 내외, 과육경도는 $2.5kg/8mm{\varphi}$로 '황금배'와 유사하였다. blade type으로 측정한 '조이스킨'의 과피경도는 22.9 N으로 '황금배' 29.9 N과 유의한 차이를 보였다. '만풍배', '신고', '황금배', '조이스킨'의 과피 석세포를 phloroglucinol-HCl로 염색하여 조사한 결과, '조이스킨'은 석세포의 크기가 작고 개수도 적은 경향을 보였다. '조이스킨'은 2012년 4월 19일에 국립종자원에 2012-311로 품종보호를 출원하였으며, 2016년 2월 품종등록(grant number, 5895) 되었다.

• 대한본초학회지
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• 제33권3호
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• pp.25-35
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• 2018

Objectives : Lepidii seu Descurainiae Semen has been frequently adulterated with the seeds of several inauthentic plant species. However, the accurate identification of these plant seeds is very difficult. To develop a reliable genetic authentication tool for Lepidii seu Descurainiae Semen, we analyzed matK sequence. Methods : To obtain the matK sequences of plant materials, genomic DNA was extracted from 24 samples and PCR amplification was carried out using matK-AF/matK-8R universal primer set and matK-LDSF/matK-LDSR primer set. For identifying species-specific nucleotides and phylogenetic analysis, matK regions were sequenced and comparatively analyzed by the ClustalW and Maximum Likelihood method. Results : We developed a new primer set to amplify matK region in Lepidii seu Descurainiae Semen and closely related plant samples. From the comparative analysis of matK sequences, we identified species-specific marker nucleotides for D. sophia, L. apetalum, L. latifolium, E. cheiranthoides, E. macilentum, and D. nemorosa, respectively. Furthermore, phylogenetic analysis revealed clear classification depending on the species. These results indicated that the matK sequence obtained a new primer set in this study was useful to identify Lepidii seu Descurainiae Semen in species level. Conclusions : We developed a primer set and identified species-specific marker nucleotides enough to distinguish authentic Lepidii seu Descurainiae Semen and adulterants at the species level based on the matK sequences. These genetic tool will be useful to prevent adulteration and to standardize the quality of Lepidii seu Descurainiae Semen.

• 국제지역연구
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• 제22권4호
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• pp.145-166
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• 2018

본 연구는 글로벌 경쟁으로 인해 지역 간의 벽은 사라지고, 고객의 욕구는 다양해지면서 시장과 기술의 급격한 변화가 일어나고 있다. 이에 미래성장 동력의 창출과 산업 경쟁력 향상을 위한 융합역량의 중요성은 점점 강조되어지고 있다. 기업들은 산업 경쟁력의 제고를 통하여 최적화된 글로벌 경영의 환경을 조성하고, 융합기술 활용을 위한 방향성과 전략수립을 통하여 기업의 효율성을 높이기 위한 혁신 수단들을 모색하여야 한다. 기업은 신제품 개발과 프로세스 혁신을 위한 내부적 자원기반인 동적역량들을 통하여 융합역량을 강화해 관련 신기술들을 최대한 수용하고 활용해야만 한다. 글로벌화된 시장과 기술에 대해서는 전략적 방향을 기업의 정형화된 기술역량에 맞추고, 프로세스에는 기술혁신에 필요한 융합역량을 반영할 때보다 높은 혁신성과를 기대할 수 있다. 시장과 기술지향성이 기술혁신성과에 미치는 영향과 동적역량과 융합역량이 기술혁신성과에 어떤 영향을 미치는지와 동적역량과 융합역량이 시장과 기술지향성과 간의 매개역할을 복합 통합적으로 실증하는 것에 초점을 맞추었다. 이를 위해 글로벌 및 다국적 기업으로 국제적 기업 활동 중 이거나 해외 지역에 본사를 두고 있는 기업들(51.4%)을 대상으로 설문을 실시하여 선행연구들을 바탕으로 가설을 수립하고 수집된 데이터를 활용 경로분석과 소벨 테스트 등을 통해 실증 분석을 실시하였다.

• 한국정보시스템학회지:정보시스템연구
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• 제18권2호
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• pp.83-109
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• 2009

Such factors as the increase of population and me development of information technology were raised the needs of citizens in Korea. To meet these needs for the better services, Korean government has built up the computer networks that connect forty-two administrative operations of the central government since 1984. Through the computerization of administrative services, Korean government has been pursuing the balanced development among the regions in the country. To this end, regional informationization has been implemented since the mid 1980s. Specifically, rural villages has become information network villages (invils) by adopting computers and networks. Consequently, three hundred thirty-seven invils were implemented in the country. By selecting forty-six invils in Kyeongbuk province in Korea, this research was intended to find efficient and effective ways of operating invils. To find the problems and opportunities of the invils, the researcher has visited each of the forty-six invils between January 12th. and February 12th. in 2009. Two-round surveys were distributed to the managers of these forty-six invils. This research identified ten problems as below. a. Problems after the implementation of invils b. Problems occurred at the same rime as the operation of invils c. Problems with regard to the invil managers d. Problems with regard to the criteria of success or failure e. Problems with regard to the cooperation of administrative offices f. Problems with regard to the boosting of invil experience g. Problems with regard to software assurance developed in invils h. Problems with regard to incentives to invlis i. Problems with regard to the role of invils To solve these problems in hands of invils, this research suggested policy ideas in two levels: 1. invils 2. government Policies should be implemented by invils: a. The strengthening of training rural people for the better utilization of computers b. The strengthening of the regulations on membership management and electronic commerce c. The establishment of the invil managers' job tenure d. The reformation of measuring the success or failure of an invil e. The integration of administrative offices centralized by invils f. The establishment of trust between administrative offices and invils g. the integration of experience villages and invil managing offices h. The revitalization of incentives to invils and experience villages i. The enforcement of cooperative offices among invils Policies to be implemented by the government: a. The revitalization of electronic commerce through invils b. The rationalization of selecting invils in an area c. The unification of various offices for rural informationization d. The construction of portal sites for rural areas e. The continuous training of IT leaders in rural areas f. The provision of pays to invil managers based on break-even points g. The transcendentalization toward the second new town movement

• 한국산학기술학회논문지
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• 제21권1호
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• pp.216-222
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• 2020

본 연구는 논에 타 작물(사료작물)인 콩과 이탈리안 라이그라스, 콩과 감자, 콩과 보리의 작부체계를 신규 도입한 농가를 비교 분석하여 시사점을 도출하고, 기술적 해결사항 등을 파악하였다. 콩 및 이탈리안 라이그라스(IRG) 논 작부체계 수익성으로는 콩의 10a당 수량은 325kg이고, kg당 판매단가는 3,962원으로 총수입은 1,288천원이었다. IRG의 10a당 수량은 1,584kg이고, kg당 판매단가는 125원으로 총수입은 198천원이었다. 콩 10a당 소득은 284천원으로 소득률은 22.1%였다. IRG 10a당 소득은 ▽30천원이었다. 콩과 감자, 콩과 보리의 10a당 총수입은 각각 930,000원, 375,000이었다. 작부체계의 유형으로는 소득형 작부체계, 조사료의 생산형, 식량자급률 제고형, 기후변화 대응형, 등 다양한 작부체계 유형이 개발되고 있으나 권역별로 다양한 작부체계 모형은 부족한 상태이며, 대체작목 선정과 지역에 맞는 유형별 작부체계의 재배기술을 더 체계적으로 확립하여야 한다. 경지 이용률과 곡물자급률 등 중장기 목표를 가지고 끊임없는 관련 연구를 통한 작부체계의 기술적 확립이 필요한 시점이라고 할 수 있다.

• 환경위생공학
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• 제7권2호
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• pp.1-10
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• 1992

Since 1960's along with industrialization and urbanization, economic growth has been . achieved, however, at the same time, environmental condition has been seriously deteriorated. . Currently, volume of wastewater has been increasing at annual rate of 7% in sewage and 20% in industrial wastewater. However, the nation's sewage treatment serves only 33% of the municipal wastewater as of 1991. Major portion of air pollutants comes from combustion of oil and coal which comprise 81% of total energy use and emission gases from motor vehicles increasing at an accelerated rate. It is known that Korea generates the highest amount of waste per capta. Nevertheless, it is not sufficient to reduce the volume of waste by means of resources recovery and recycling. Recognizing the importance of global environmental problems such as ozone layer depletion, global warming and acid rain, international society has been making various efforts since the 1972 Stockholm conference. In particular, it is expected that the Rio conference which has adopted the Rio declaration and Agenda 21 will form a crucial turning point of the emerging new world order after the Cold War confrontation. To cope with such issues as domestic pollution and global environmental problems, the fundamental national policy aims at harmonizing "environmental protection and sustainable development". The Ministry of Environment has recently set up a mid-term comprehensive plan which includes annual targets for environmental protection. According to the government plan, gradual improvement of various environmental conditions and specific measures to achieve them is planned in time frame. Additional sewage treatment plants will be constructed in urban areas with the target to treat 65% of the nation's municipal sewage by 1996. Supply of clean fuels such as LNG will also be expanded starting from large cities as a cleaner substitute energy for coal and oil. In parallel with expansion of LNG, emphasis will be placed on installation of stack monitoring system. Due to the relatively limited land, government's basic policy for solid waste treatment is to develop large scale landfill facilities rather than small sized ones. Thirty three regional areas have been designated for the purpose of waste management. For each of these regions, big scale landfill site is going to be developed. To increase the rate of waste recycling the government is planning to reinforce separate collection system and to provide industries with economic incentives. As a part of meeting the changing situation on global environmental problems after UNCED, and accommodation regulatory measures stipulated in the global environmental conventions and protocols, national policy will try to alter industrial and economic structure so as to mitigate the increasing trends of energy consumption, by encouraging energy conservation and efficiency. In this regard, more attention will be given to the policy on the development of the cleaner technology. Ultimately, these policies and programs will contribute greatly to improving the current state of national public health.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1262-1262
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• 2001

The rice plant is one of the important staple crops in Korea. The high yield with low cost in rice is required the soil fertility and the development of new precise method of fertilizer application by nutritional diagnosis. Now, in Korea, the nitrogen application system for the rice plant is composed of the basal fertilization, fertilization at tillering stage and fertilization at panicle stage, which the nitrogen fertilization at panicle stage amount to about 30 percent in the total amount. Thus, this experiment carried out to the development of the system that can measure the nitrogen content in the rice plant at panicle stage rapidly with the near infrared spectroscopy, and to predict the appropriate quantity of the nitrogen fertilization at panicle stage based on calibration model for test of nitrogen content in rice plant. The samples were collected from 48 varieties in 4 regions which are mainly cultivated in the southern part of Korea. And then, it collected by classifying into the leaf, the whole plant and the stem since 7 days before the nitrogen fertilization at panicle stage. The ranges of the nitrogen contents were 1.6∼4.0%, 1.7∼3.0% and 1.4∼2.7% in the leaf, the whole plant and the stem, respectively. In the calibration models created by each part of the plant under the Multiple Linear Regression(MLR) method, the calibration model for the leaf recorded the relatively high accuracy. The mutual crossing test on unknown samples were carried out using Partial Least Square(PLS) calibration model. That is, the nitrogen content in the stem was tested by calibration model made by the leaf model and that of stem was tested by calibration model made by whole plant sample. When unknown leaf sample was tested by calibration model made by all sample that collected from each part in rice plant such as leaf, stem and whole plant, it recorded the highest accuracy. As a result, to test the nitrogen content in the rice plant at panicle stage, the nitrogen content in the leaf shall be tested by the calibration model composed of the leaf, the stem and the whole plant. In future, to estimated the amount of nitrogen fertilization at panicle stage for rice plant , it will be calculated based on regression model between rice yield and nitrogen content of leaf measured by calibration model made by mixed sample including leaf, stem and whole plant.

• 농촌계획
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• 제30권2호
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• pp.13-24
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• 2024

This study conducts an analysis of social media big data pertaining to island tourism resources, aiming to discern the diverse forms and categories of island tourism favored by consumers, ascertain predominant resources, and facilitate objective decision-making grounded in scientific methodologies. To achieve this objective, an examination of blog posts published on Naver from 2022 to 2023 was undertaken, utilizing keywords such as 'Island tourism', 'Island travel', and 'Island backpacking' as focal points for analysis. Text mining techniques were applied to sift through the data. Among the resources identified, the port emerged as a significant asset, serving as a pivotal conduit linking the island and mainland and holding substantial importance as a focal point and resource for tourist access to the island. Furthermore, an analysis of the disparity between existing island tourism resources and those acknowledged by tourists who actively engage with and appreciate island destinations led to the identification of 186 newly emerging resources. These nascent resources predominantly clustered within five regions: Incheon Metropolitan City, Tongyeong/Geoje City, Jeju Island, Ulleung-gun, and Shinan-gun. A scrutiny of these resources, categorized according to the tourism resource classification system, revealed a notable presence of new resources, chiefly in the domains of 'rural landscape', 'tourist resort/training facility', 'transportation facility', and 'natural resource'. Notably, many of these emerging resources were previously overlooked in official management targets or resource inventories pertaining to existing island tourism resources. Noteworthy examples include ports, beaches, and mountains, which, despite constituting a substantial proportion of the newly identified tourist resources, were not accorded prominence in spatial information datasets. This study holds significance in its ability to unearth novel tourism resources recognized by island tourism consumers through a gap analysis approach that juxtaposes the existing status of island tourism resource data with techniques utilizing social media big data. Furthermore, the methodology delineated in this research offers a valuable framework for domestic local governments to gauge local tourism demand and embark on initiatives for tourism development or regional revitalization.