• Journal of Life Science
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• v.25 no.6
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• pp.680-685
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• 2015

Previously, cellulase and xylanase producing microorganism, Bacillus subtilis NC1, was isolated from soil. Based on the 16S rRNA gene sequence and API 50 CHL test the strain was identified as Bacillus subtilis, and named as B. subtilis NC1. We cloned and sequenced the genes for cellulase and xylanase. Plus, the deduced amino acid sequences from the genes of cellulase and xylanase were determined and were also identified as glycosyl hydrolases family (GH) 5 and 30, respectively. In this study to optimize the medium parameters for cellulase production by B. subtilis NC1 the RSM (response surface methodology) based on CCD (central composite design) model was performed. Three factors, tryptone, yeast extract, and NaCl, for N or C source were investigated. The cellulase activity was measured with a carboxylmethyl cellulose (CMC) plate and the 3,5-dinitrosalicylic acid (DNS) methods. The coefficient of determination (R²) for the model was 0.960, and the probability value (p=0.0001) of the regression model was highly significant. Based on the RSM, the optimum conditions for cellulase production by B. subtilis NC1 were predicted to be tryptone of 2.5%, yeast extract of 0.5%, and NaCl of 1.0%. Through the model verification, cellulase activity of Bacillus subtilis NC1 increased from 0.5 to 0.62 U/ml (24%) compared to the original medium.

• Korean Journal of Computational Design and Engineering
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• v.12 no.1
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• pp.39-49
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• 2007

Recently three-dimensional (3-D) die design and production process has been widely introduced into the tooling shops of automotive manufacturers to reduce time-to-production of brand-new automobiles. 3-D solid models created in CAD systems are used not only for various simulations for design verification, but also for NC tool path generation to machine dies and their Styrofoam patterns. However, a lot of time and cost will be required to build solid models for dies if designers use only the generalized modeling capabilities of commercial 3-D CAD systems. To solve this problem, it is necessary to customize 3-D CAD system for the specific die design and manufacturing process. This paper describes a dedicated 3-D CAD system based on Unigraphics for die design for automotive body panels. The system provides not only 3-D design capabilities, but also standard part libraries, to enhance design productivity. The design process modeling technology has been introduced to facilitate redesign of the die for the modified panel. By introducing this system, dies can be designed more rapidly in the 3-D space, and their solid data can be directly transferred to CAM tools for NC tool path generation and simulation tools for virtual manufacturing.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1424-1428
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• 2005

This paper describes a dedicated three-dimensional CAD system for design of drawing dies for automotive body panels. Since solid die models are useful not only for simulations for design verification, but also for NC tool path generation to machine dies and their Styrofoam patterns, 3-D CAD systems have been introduced in the tooling shop of automotive manufacturers. However, the work to build solid models requires a lot of time and effort if the designer uses only the general modeling capabilities of commercial 3-D CAD systems. To solve this problem, we customized a 3-D CAD system for the drawing die design. The system provides not only 3-D design capabilities, but also standard part libraries to enhance design productivity. By introducing this system, the drawing dies can be designed more rapidly in the 3-D space, and their solid data can be directly transferred to CAM tools for NC tool path generation and simulation tools for virtual manufacturing

• Journal of the Society of Naval Architects of Korea
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• v.44 no.2 s.152
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• pp.174-179
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• 2007

The cutting automation of boat's wooden pattern is strongly required to improve the productivity and quality of boats in leisure boat industry. This paper is concerned with the development of wooden pattern machining technology by the machining center. The leisure boat is designed with a 3 dimensional design s/w. The NC cutting data are generated in a CAM s/w and are verified using verification s/w. The cutting forces are monitored to analyse the cutting process. The surface characteristics of machined surface are investigated at various cutting conditions such as spindle speed, feed speed, the cutting direction of wood, and wood material.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.10a
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• pp.71-76
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• 2001

In the surface measurement system using touch probe, probe radius compensation is a key factor for accuracy. In this paper we investigate methods for compensating probe radius so that the surface equation for an "unknown surface" can be efficiently derived. The developed algorithm derives the surface equation by the iterative procedure of estimation, verification, and modification . Since the procedure is applied only for the surface region exceeding the tolerance limit, an accurate surface equation can be obtained with less computation and measurement point. The validity and effectiveness of the algorithm was tested by numerical simulations. The results convinced us that the develop algorithm can be used for surface measurement and tool path planning for NC machining.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.5
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• pp.47-56
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• 2003

Geometric cutting-simulation and verification play an important role in detecting NC machining errors in mold & die manufacturing and thereby reducing correcting time & cost on the shop floor. Current researches in the area may be categorized into view-based, solid-based, and discrete vector-based methods mainly depending on workpiece models. Each methodology has its own strengths and weaknesses in terms of computing speed, representation accuracy, and its ability of numerical inspection. The paper proposes a hybrid modeling scheme for workpiece representation with z-map model and discrete vector model, which performs 3-axis and 5-axis cutting-simulation via tool swept surface construction by connecting a sequence of silhouette curves.

• Korean Journal of Computational Design and Engineering
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• v.4 no.3
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• pp.247-258
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• 1999

With the contemporary CAD/CAM system, where the tool path is generated and verified purely based on the geometric operation, geometric accuracy of the machined surface cannot be guaranteed dut to the cutting mechanics, meaning that the cutting mechanics should be incorporated in some fashion. In this paper, we incorporate the instantaneous cutting force and the tool deflection phenomena in predicting the machined surface for the finish-cut and milling operation. For the given NC dat including cutting conditions, the developed algorithm computes cutting force and deflection amount along the tool trajectory, and outputs the 3D graphic model of the machined surface together with error analysis. The validity and accuracy of the presented method has been tested by the actual cutting experiments. Experimental results and accuracy enhancement method together with implementing architecture of the VMCS (Virtual Machining CAM System) are discussed in the paper.

• Journal of the Korean Society of Industry Convergence
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• v.4 no.4
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• pp.365-369
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• 2001

This paper presented a new model of virtualized CNC milling machine. The model verifies the over cut, the under cut and the surface roughness using NC file generated from CAM and cutting condition. The model uses Z-map model to verify workpiece. In this paper, the model used the velocities of x, y and z direction and obtained a center point of a hall end mill for modeling Z-map of workpiece. To investigate the performance of the model, simulation study was carried out. As the results, the model gave geometry accuracy of workpiece, the surface roughness and the chip loads in finish cutting that can predict tool chipping. The virtualized CNC machine can he used a flat end mill, a ball end mill and a rounded end mill.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2002.02a
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• pp.3-10
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• 2002

In this study on machine measuring system based on solid feature was developed. This system was applied with injection mold using 3 dimensional solid modeler for verification. Developed program include pre-processor, main processor, and post processor. In pre-processor there are functions which check intersection, simulate motion of probe and calculate measuring time. Main processor generates measuring path and output NC code in Unigraphics. In post-processor functions that include evaluation of undercut or overcut and display of measuring procedure are offered. In addition analysis module for quality control of measured data on manufactured product was developed with geometric and dimensional tolerance concept. As the result developed program could get stability of system, precision of product, rapidity and cost down of manufacturing process compared with before measuring process.

• The Transactions of the Korean Institute of Power Electronics
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• v.11 no.3
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• pp.274-283
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• 2006

The Permanent Magnet Synchronous Motor(PMSM) drive systems with ball-screw, gear and timing-belt are widely used in industrial applications such as NC machine, machine tools, robots and factory automation. These systems have torsional vibration in torque transmission from servo motor to mechanical load due to the mechanical couplings. This vibration makes it difficult to achieve quick responses of speed and may result in damage to the mechanical plant. This paper presents adaptive notch filter with auto searching function of vibration frequency to reject the mechanical vibration of linear feeder system with PMSM. The proposed adaptive notch filter can suppress the torque command signal of PMSM in the resonant bandwidth for reject the mechanical torsional vibration. However, the resonant frequency can vary with conditions of mechanical load system and coupling devices, adaptive notch filter can auto search the vibration frequency and suppress the vibration signal bandwidth. Computer simulation and experimental results shows the verification of the proposed adaptive notch filter in linear feeder system with PMSM.