• Title/Summary/Keyword: NAGase

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Diagnosis of Bovine Subclinical Mastitis by the Measurement of the N-acetyl-$\beta$-D-glucosaminidase Activity and Effect of Levamisole Treatment (N-acetyl-$\beta$-D-glucosaminidase 활성치에 의한 젖소의 준임상형유방염의 진단과 염산 Levamisole의 치료시험)

  • Kang Byong-Kyu;Yang Gun-Muug
    • Journal of Veterinary Clinics
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    • v.5 no.1
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    • pp.23-30
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    • 1988
  • A total of 127 foremilk samples from dairy farms in Chonnam district was examined for the subclinical mastitis eve. six months, using a method of the N-acetyl-${\beta}$ -D-glucosaminidase (NAGase) test in relation to the California mastitis test(CMT) and the somatic tell count(SCC) and the compatibility and efficiency rating between the NAGase test and the other screening test were conducted. Fifteen subclinically mastitic cows were treated with a single oral dose of 7.5mg/kg of levamisole hydrochloride. The results are summarized as follows. 1. A linear relationship was found among the NAGase level, the CMT score and the SCC level, and it was found that NAGase activity measurements were comparable with other screening tests for diagnosing cows with mastitis. 2. Compatibilites between the NAGase and the CMT were 96.1%, and that of the NAGase and SCC were 93.7%. On the other hand, relative efficiency ratings of Postle's equation between the NAGase and CMT were 89.4%, and that of the NAGase and SCC were 84.1%. 3. Considering the result of CMT, SCC and NAGase level after treatment with levamisole hydrochloride it seemed to be of special value in the treatment of bovine subclinical mastitis.

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Relationship between the N-acetyl-β-D-glucosaminidase levels and the presence of mastitis pathogens in bovine mastitis milk samples (유우(乳牛) 유방염(乳房炎) 진단(診斷)에 있어서 N-acetyl-β-D-glucosaminidase 치(値)와 분리(分離) 원인균(原因菌)과의 관계(關係))

  • Kang, Byong-kyu;Nam, Hyang-mi;Son, Chang-ho
    • Korean Journal of Veterinary Research
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    • v.33 no.3
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    • pp.531-537
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    • 1993
  • A study was carried out to define the relationship between the N-acetyl-$\beta$-D-glucosaminidase(NAGase) levels and isolated pathogenic bacteria in 379 quarter fore milk of mastitis suspected samples collected in this clinics. All samples were tested the NAGase, California mastitis test(CMT), Somatic cell count(SCC) and bacterial culture. Except 111 from 379 samples, 268 bacteria-positive quarter fore milk samples were classified into the latent and mastitis infection group by SCC($500,000cells\;per\;m{\ell}$), and the mean NAGase levels($nmol/min/m{\ell}$) of each isolated pathogen in mastitis infection group were Staphylococcus aureus 3.067, Coagulase-negative staphylococci 4.083, Staphylococcus aureus 3.594, Str. uberis 3.513, Str. dysgalactiae 1.640, E coli 4.441 and gram negative rods 4.560, respectively. Most of the relationship between mean SCC and NAGase in each pathogen group were highly significant using a student t test(p<0.05). When the mastitis pathogens were classified into minor(Coagulase-negative staphylococci, Corynebacterium sp.) and major pathogen group(Staphylococcus aureus, Streptococcus agalactiae, Str. uberis, Str. dysgalactiae, gram negative rods), the NAGase levels were higher at major than minor pathogen group. On the other hand, when the mastitis milk samples were classified by SCC($500,000cells\;per\;m{\ell}$) and by the presence of pathogen(IDF scheme), the NAGase levels were also higher at the mastitis than latent infection. The possibility of combining SCC and NAGase data in order to give the more difinitive diagnosis is discussed.

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STUDY OF CATALYSIS FOR MAKING ALCOHOL FROM ACROLEIN AND ISOPROPYL ALCOHO

  • Nagase, Yoshinori
    • Journal of the Korean institute of surface engineering
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    • v.29 no.6
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    • pp.640-643
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    • 1996
  • The vapor phase hydrogen trancefer reaction between acrolein and isopropyl alcohol has been investigated over $Ag_2O$-Cao catalyst. A high selectivity of allyl alcohol was obtained at 88.7% with conversion of 75.4%.

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Separating a Chart

  • Nagase, Teruo;Shima, Akiko
    • Kyungpook Mathematical Journal
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    • v.57 no.1
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    • pp.163-185
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    • 2017
  • In this paper, we shall show a condition for that a chart is C-move equivalent to the product of two charts, the union of two charts ${\Gamma}^*$ and ${\Gamma}^{**}$ which are contained in disks $D^*$ and $D^{**}$ with $D^*{\cap}D^{**}={\emptyset}$.

Enzymatic Determination of Somatic Cells by Using Transparisation in Raw Milk

  • Lee, Bou-Oung;Xu, Wen-Ying;Chang, Oun-Ki;Jin, Tai-Hua
    • Food Science of Animal Resources
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    • v.24 no.4
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    • pp.411-415
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    • 2004
  • The transparisation technology for milk and milk products could be applied widely and very importantly to various determination because transparisation can economize the cost and increase with precision in the milk payment system. Component of butanone or Triton in transparisation solvent would inhibit the growth of bacteria and method. Enzymatic determination of leucocytes were proposed to evaluate milk quality as mastitis in the milk payment system, this can be easily applied to simplify automation of the determation with the lowest investment cost in milk pay system. The significance of this technique, it can be used in the quality control of raw milk and milk products, milk payment system, and programming of national dairy project. Transparisation technology is used in somatic cell counting by enzymic methods. The range of deviation for this method is 16% in 74 samples. But the deviation is increased to 20% when the Infoss method is used. It is affected by the percentage of epithelial cells and white blood cells in somatic cells from different animals and the stages of aging. NAgase activity has an obvious correlation with white-blood cells in milk. In the case of mastitis the white-blood cells is 90-95% in somatic cells in milk, it is showing greater precision in measuring the state of mastitis. In conclusion the enzymic method of somatic cell counting is a relatively simple and easy method of measurement and can be easily practiced. And the importance of this method is also worth utilizing for indirect counting of Somatic cells by use of synthetic substrates to NAgase. In the future, with the further development of the research in this field, it will b possible to automatize the measurement.

Fundamental Study of nanoDot OSL Dosimeters for Entrance Skin Dose Measurement in Diagnostic X-ray Examinations

  • Okazaki, Tohru;Hayashi, Hiroaki;Takegami, Kazuki;Okino, Hiroki;Kimoto, Natsumi;Maehata, Itsumi;Kobayashi, Ikuo
    • Journal of Radiation Protection and Research
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    • v.41 no.3
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    • pp.229-236
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    • 2016
  • Background: In order to manage the patient exposure dose in X-ray diagnosis, it is preferred to evaluate the entrance skin dose; although there are some evaluations about entrance skin dose, a small number of report has been published for direct measurement of patient. We think that a small-type optically stimulated luminescence (OSL) dosimeter, named nanoDot, can achieve a direct measurement. For evaluations, the corrections of angular and energy dependences play an important role. In this study, we aimed to evaluate the angular and the energy dependences of nanoDot. Materials and Methods: We used commercially available X-ray diagnostic equipment. For angular dependence measurement, a relative response of every 15 degrees of nanoDot was measured in 40-140 kV X-ray. And for energy dependence measurement, mono-energetic characteristic X-rays were generated using several materials by irradiating the diagnostic X-rays, and the nanoDot was irradiated by the characteristic X-rays. We evaluated the measured response in an energy range of 8.1-75.5 keV. In addition, we performed Monte-Carlo simulation to compare experimental results. Results and Discussion: The experimental results were in good agreement with those of Monte-Carlo simulation. The angular dependence of nanoDot was almost steady with the response of 0 degrees except for 90 and 270 degrees. Furthermore, we found that difference of the response of nanoDot, where the nanoDot was irradiated from the randomly set directions, was estimated to be at most 5%. On the other hand, the response of nanoDot varies with the energy of incident X-rays; slightly increased to 20 keV and gradually decreased to 80 keV. These results are valuable to perform the precise evaluation of entrance skin dose with nanoDot in X-ray diagnosis. Conclusion: The influence of angular dependence and energy dependence in X-ray diagnosis is not so large, and the nanoDot OSL dosimeter is considered to be suitable dosimeter for direct measurement of entrance surface dose of patient.