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Effect of Aeration and Agitation Conditions on the Production of Glucoamylase with Aspergillus niger No. PFST-38

  • Oh, Sung-Hoon;O, Pyong-Su;Lee, Cherl-Ho
    • Journal of Microbiology and Biotechnology
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    • v.3 no.4
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    • pp.292-297
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    • 1993
  • Aspergillus niger No. PFST-38 was grown on complex media in 30L agitated fermentors at various aeration rates and stirrer speeds. We could correlate the mixing time as a function of the Reynolds number and the apparent viscosity, as follows. ${\theta}_M=2.95\;\NRe^{-0.52},\;{\theta}_M=1.88\;{\eta_a}^{0.57}$ Also, the effects of the apparent viscosity (${\theta}_a$), the impeller rotational speed (N), the air flow rate ($V_s$), and the mixing time (${\theta}_M$) on the oxygen transfer coefficient, $K_L a$ were determined experimentally, and equated as follows. $K_La=12.04N^{0.88}Vs^{0.71}{n_a}^{-0.83},\;K_La=30.2N^{0.88}Vs^{0.71}{\theta_M}^{-1.45}$ $K_La$ increased as the agitation speed and the air flow rate increased. The rate of $K_La$ increase was dependent more on the rotational speed of impeller than on the air flow rate. The glucoamylase production increased with the increase of the agitation speed upto at 500 rpm and increased with the increase of air flow rate upto at 1.0 vvm. The values calculated from the above equation confirmed that the experimental maximum production of glucoamylase was achieved when the $K_La$ and the apparent viscosity of the broth were $260\;hr^{-1}$ and 1800 cps, respectively.

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Recycling of Cutting Oil from Silicon Waste Sludge of Solar Wafer (태양광용 웨이퍼 실리콘 폐슬러지로부터 절삭유의 재생)

  • Um, Myeong-Heon;Lee, Jong-Jib;Ha, Beom Yong
    • Clean Technology
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    • v.22 no.4
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    • pp.274-280
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    • 2016
  • In this study, it was to develop a chemical method that can recycle the cutting oil which accounts for about 25% of the cost of the process among containing materials of silicon waste sludge generated in the process for producing a solar cell wafer. The 7 types of reagents have been used, including acetone, HCl, NaOH, KOH, $Na_2CO_3$, HF, $CH_2Cl_2$, etc. for this experiment. And It was carried out at a speed of 3000 rpm for 60 minutes centrifugation after performing a reaction with a waste sludge at various concentrations. As a result, the best reagents and conditions for separating the solid such as a silicon powder and a metal powder and liquid cutting oil were identified as 0.3 N NaOH. It is found to be pH 6.05 in a post-processing recycled cutting oil with 0.3 N NaOH after reaction of waste sludge and 0.1 N HCl which is effective to remove metal powder in order to adjust the pH to suit the properties of the weak acid is a commercially available cutting oil and it showed excellent turbidity than when applied to sludge with 0.3 N NaOH alone. The results of FT-IR analysis which can compare the properties of the commercially available cutting oil shows it has a possibility of recycling oil. The cutting oil recovery rate obtained through the experiment was found to be 86.9%.

Inhibition of Nitric Oxide Production and Hyaluronidase Activities from the Combined Extracts of Platycodon grandiflorum, Astragalus membranaceus, and Schisandra chinensis (길경, 황기와 오미자 혼합추출물의 NO 억제활성과 Hyaluronidase 억제활성 효과)

  • Kang, Chang-Ho;Kwak, Dae Young;So, Jae-Seong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.6
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    • pp.844-850
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    • 2013
  • In this study, the optimal extraction conditions for three medicinal herbs as functional sources against inflammatory and arthritic diseases were developed. Traditional medicinal herbs were screened for their inhibition of hyaluronidase (HAse) activity and nitric oxide (NO) synthesis. For the screening of anti-inflammatory properties, ethanolic extracts of 53 species of traditional medicinal herb were examined. We confirmed that Astragalus membranaceus (A.R.), Schisandra chinensis (S.F.), and Platycodon grandiflorum (P.G.) inhibit NO production. For extraction from all three herbs simultaneously, an ethanol concentration of 95%, a 1:2:1 mixture ratio, and at 50 rpm mixing speed, for over 12 h and at $30^{\circ}C$ was the best condition for optimal extract yield and NO inhibition effects. HAse inhibition from the three herb extraction was three fold higher than single samples. The ethanol extracts were fractionated with various solvents (n-hexane, chloroform, ethyl acetate, n-butanol, and water). The ethyl acetate-soluble fraction of the herb mixture showed the highest extract yield (13%) and NO inhibition effects (73%). In conclusion, this study provides experimental evidence that a mixture of P.G., A.R., and S.F. could be used as a source of antioxidant ingredients in the food industry.

Rubber bound phenolic antioxidant and its application in thermoplastic elastomer

  • Klinpituksa, Pairote;Kiarttisarekul, Anyarat;Kaesaman, Azizon
    • Advances in materials Research
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    • v.3 no.3
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    • pp.175-183
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    • 2014
  • Natural rubber bound phenolic antioxidant, 2,6-di-tert-butyl-4-vinylphenol (2,6-DBVP), was prepared from natural rubber and 2,6-DBVP in both solution and melt state. The 2,6-DBVP had been synthesized from 3,5-di-tert-butyl-4-hydroxybenzaldehyde and methyltriphenylphosphonium iodide ($MePPh_3I$) by Wittig reaction ($0^{\circ}C$ for 2 hrs, $N_2$ atmosphere). The conditions for preparation of natural rubber bound 2,6-DBVP (NR-DBVP) were optimized for both solution state (1 phr BPO and 8 phr 2,6-DBVP at $70^{\circ}C$ for 2 hrs) and for melt state (1 phr BPO and 8 phr 2,6-DBVP at $70^{\circ}C$ for 10 mins, with rotor speed of 60 rpm). A thermoplastic vulcanizate was obtained using a compatibilizer, polypropylene modified with phenolic resin (PhHRJ-PP), in a closed mixer ($180^{\circ}C$ for 3 mins, rotor speed 60 rpm). The antioxidant properties of vulcanized NR-DBVP, using phenolic as the vulcanization system, were similar to NR with the conventional antioxidant BHT. In addition, the antioxidant, water leaching property of the thermoplastic vulcanizate of NR-DBVP/PP were good in comparison to a NR blend with BHT; the morphologies of these thermoplastic vulcanizates were similar.

Optimization of Friction Stir Spot Welding Parameters of Lap Joint between AA1100 Aluminum Alloy and SGACD Zinc-coated Steel

  • Triwanapong, Surat;Kaewwichit, Jesada;Roybang, Waraporn;Kimapong, Kittipong
    • International Journal of Advanced Culture Technology
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    • v.3 no.1
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    • pp.161-168
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    • 2015
  • This article aims to apply a friction stir spot welding for producing a lap joint of AA1100 aluminum alloy and SGACD zinc coated steel. The experiment was designed by MINITAB and then investigated the relation among the friction spot joint parameters. The experimental results are as follows. The friction spot joining could successively produce the lap joint of AA1100 aluminum alloy and SGACD zinc coated steel. Interaction between the rotate speed, the hold time and the tool insert speed affected to vary the tensile shear strength of the lap joint. The prediction of the optimized welding parameters that indicated the tensile shear strength of 1966 N was the rotated speed of 4000 rpm, the pin hold time of 6 sec, the pin insert rate of 6 mm/min with the S/N ratio of 66.56 that was higher than that of the total mean S/N ratio. The practical experiment of the predicted welding parameters indicated the tensile shear strength of 2165 N and had the S/N ratio of 66.70 that was higher than the predicted tensile shear strength.

Optimization of Environmental Parameters for Extracellular Chitinase Production by Trichoderma harzianum SJG-99721 in Bioreactor (Trichoderma harzianum SJG-99721의 체외 분비 chitinase 생산에 미치는 생물 반응기에서의 반응 최적화 연구)

  • 이호용
    • Korean Journal of Environmental Biology
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    • v.22 no.1
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    • pp.167-170
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    • 2004
  • A self-directing optimization procedure was applied to determine the best environmental factors in operating the bioreactor. The self-directing optimization process was employed to determine the best conditional combination of multi parameters, pH, temperature, aeration rate and mixing rate toy maximal production of chitinase by Trichoderma harzianum SJG-99721 in batch mode fermentation. Among these factors, the parameters of pH and aeration rate were found to be particularly important on mycellial growth and chitinase activity. pH 4.89, an aeration rate of 3.22 ι per minute and an agitation rate of 225 rpm was found to be the best combination. By the optimization, chitinase activity was dramatically increased from an initial value of 4.221 U under basic conditions to n final value of 16.825 U.

SLURRY UTILIZATION SYSTEM IN THE PADDY FIELD

  • I. H. Oh;Kim, K.D.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2000.11c
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    • pp.784-791
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    • 2000
  • To improve the uniformity of slurry spreading and to reduce the odor problem, a hose slurry spreader was developed, which spread the slurry near to the surface of the soil. First, the uniformity of slurry spreading was investigated. The best result of 8.1 % CV was obtained at the rotor speed of 250 rpm than any other speeds for the swine slurry, which contains 13.6% of dry matter. In case of dairy cattle slurry, which contains 8.2% of dry matter, the uniformity has the best result of CV 7.2% by high rotor speed of 330 rpm. A high speed of rotor has built a sufficient pressure in the distributor and discharged a uniform quantity of slurry through the hoses. Therefore, in practical use one should work with rotor speed over 300 rpm to maximize the uniformity of slurry spreading. Second, odor test was done with the swine slurry. While the conventional spreader shows ∑ value 440, the hose slurry spreader and its combination of disk harrow show ∑ value 258 and 184 respectively. With the air dilution sensual test and a 3-point odor bag, the conventional spreader shows 66.9 or 35.4 point and by the hose slurry spreader is 9.7 or 11.1 point. So, the developed spreader was found to have a greater effect on the reduction of odor problem. Finally, it is recommended to spread certain amount of the slurry for paddy field equivalent to the chemical fertilizer based on the N-content. It means 22 tons of swine slurry per ha. Since most of the livestock farms possess less arable land, a system of linking farms is necessary to utilize the slurry crossover the farms. The cost of slurry utilization including filling, transport spreading and brokerage is 3200-6800 Won/㎥ in accordance with the transport distance.

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Target motion analysis of the respiratory gated guided radiotherapy in liver cancer patients using 4D-CT (4D-CT와 호흡동조시스템을 이용한 간암 환자의 방사선치료 표적 움직임 분석)

  • Dong, Kyung-Rae;Park, Byung-Soo;Kim, Sae-Sark;Kweon, Dae-Cheol;Goo, Eun-Hoe;Chung, Woon-Kwan
    • Journal of Radiation Protection and Research
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    • v.35 no.2
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    • pp.63-68
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    • 2010
  • The ultimate goal of radiation treatment is to use enough radiation dosage in order to examine a tumor while protecting normal tissue. Respiratory guided radiotherapy is being clinically implemented to examine a given stabilized area in order to compensate for the problems of patient breathing. This study investigates the effects of breathing movements on 40 patients with liver cancer through the actual radiation therapy plan using 4D-CT and respiratory guided radiotherapy using RPM. Using a commercial RPM respiratory gating system 4D-CT, we acquired 4D CT on multislice helical CT scanners that use different approaches to 4D CT image reconstruction. The results from analyzing forty patients according to age and direction showed no relationship between gender and transition change. The mean left-right, anteroposterior, and craniocaudal total movements were $3.19{\pm}1.29$, $5.44{\pm}2.07$, and $12.54{\pm}4.70$ mm, respectively. Changes were the largest with CC directions and as patients advanced in age, movements were larger. Therefore, as changes occur in treatment areas because of movements caused from breathing, respiratory gating system is put into operation to revise movement and can increase the radiotherapeutics effects in treating liver cancer.

MOCVD growth of GaN and InGaN in a rotating-disk reactor

  • 문용태;김동준;김준형
    • Proceedings of the Korean Vacuum Society Conference
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    • 1998.02a
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    • pp.109-109
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    • 1998
  • 최근 들어 MOCVD 법으로 성장시킨 GaN, InGaN, AIGaN를 이용한 광소자 ( (LED, LD)와 전자소자(FET, MODFET)에 대한 관심이 고조되면서, MOCVD 법 을 이용한 GaN 중심의 질화물 반도체 성장에 관심이 집중되고 있다. 금번 실험에 사용된 MOCVD 장비는 수직형 MOCVD 장비이다. 특히, wafer c carner를 1$\alpha$)() rpm이상의 고속으로 회전시킬 수 있는 장치로서 원료 가스의 반웅 기 내에서의 흐름을 균일하게 하여 uniformity가 높은 질화물 반도체를 성장시킬 수 있다 .. GaN 에피충은 c-plane 사파이어를 기판으로 하여 11 00 "C 이상의 고온 에서 수소를 이용하여 기판을 cleaning하고, 500 "C 부근에서 핵생성충올 성장시 킨 후 1050 "C에서 trimethylgallium(TMGa)과 NI-h를 이용하여 성장시켰다. n n -GaN를 성장시키기 위해서는 SiH4을 사용하였으며, InGaN의 경우는 t trimethylindium(TMIn)을 In원 료 가스로 하여 635 - 725 "C 범 위 에 서 성 장시 켰 다. 성 장된 undoped GaN, n-GaN, InGaN는 X -ray di잔raction(XRD), H떠l m measurement, Photoluminescence(PU동올 이용하여 결정성과 전기적 및 광학적 특성올 고찰하였다 .. 2ttm 두께로 성장된 undoped G값V박막의 경우 Hall 측정결과 6 6 X lOI6/e며 정도의 낮은 도핑 농도를 보였으며, V!lII ratio(2500 - 5000)증가에 따라 결정성이 향상됨을 GaN (102)면의 X -ray e -rocking분석올 통하여 확인하 였다 .. n-GaN의 경우 SiH4양올 3 - 13 sccm으로 증가시킴에 따라 n -type 도명농 도가 선형적으로 증가하였고, 1017/c며 범위 내로 도평이 된 경우 상온에서 300 e마 N Ns 이상의 high mobility를 얻올 수 있었다 .. PL 관측 결과로부터 Si 도핑으로 인 하여 GaN bandedge emission이 강화됨을 알 수 있었다 .. InGaN 박막의 경우 성 장온도를 낮춤에 따라서 m의 양을 증가시킬 수 있었다. 또한 유량비(TMIn I T TMGa)가 1에 가까운 경 우에서도 온도를 635 "C 정도로 낮훈 경우 410 nm정도에 서 PL bandedge peak올 얻을 수 있었으며, 이 때의 반치폭은 50 meV정도의 낮 은 값을 보였다. 반치폭은 50 meV정도의 낮 은 값을 보였다.

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Study on the Activation of Microbial Products by Using the Leachate (침출수를 이용한 미생물 제제의 활성에 관한 연구)

  • 이장훈;정준오;남명흔
    • Journal of Environmental Health Sciences
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    • v.24 no.3
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    • pp.70-76
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    • 1998
  • Activation bacteria, identified from commercial microbial products, were applied to leachate treatment. Total seven strains of bacteria Enterobacteriaceae spp. (5), Bacillus sp. (1), Aeromonas sp. (1) were seeded in the leachate and cultured in the shaking incubator at 25$^{\circ}$C and 250 rpm. While cultured, they were sampled in given time intervals and the removal rates of SS, COD, BOD, T-N.and T-P were measured an indicators of leachate treatment. Through the screening test, four of 7 strains of bacteria were considered to be effective and they were named as "effective group". The capability of leachate treatment was observed on three different groups of bacteria single, effctive, and total mixed. The result showed that the removal rates of COD and SS for the total mixed group were 64 and 71% respectively. BOD removal rate was reached nearly 99% by seeding of effective griup and removal rates of T-P and T-N were 83 and 82% respectively. However seeding of single strain was less effective than that of any mixed group in leachate treatment.

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