• Title/Summary/Keyword: N-fixation

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Can bone marrow aspirate improve mandibular fracture repair in camels (Camelus dromedarius)? A preliminary study

  • Al-Sobayil, Fahd;Sadan, Madeh A.;El-Shafaey, El-Sayed;Ahmed, Ahmed F.
    • Journal of Veterinary Science
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    • v.21 no.6
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    • pp.90.1-90.11
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    • 2020
  • Background: Mandibular fractures are common in camels, leading to considerable economic losses. This study explored methods of improving mandibular fractures repair, adjuvant with interdental wire, or bone plate fixation. Autologous bone marrow (BM) injection enhances osteogenesis and rapid healing. Objectives: To investigate the effect of autologous BM aspirate as an adjuvant treatment for repairing mandibular fractures in camels with interdental wire, or bone plate fixation. Methods: Thirty dromedary camels aged 5-8 years and of both sexes were randomly divided into 4 treatment groups: group 1 (n = 10) treated with stainless steel wire fixation and BM injection at the fracture line, group 2 (n = 10) treated with plate fixation and BM injection at the fracture line, group 3 (n = 5) treated with stainless steel bone wire fixation and placebo saline injection at the fracture line, and group 4 (n = 5) treated with plate fixation and placebo injection at the fracture line. The mandibular fractures were followed weekly for 12 weeks postoperatively to assess improvement and healing based on clinical evaluation, radiographic union scale, and bone turnover markers (i.e., bone alkaline phosphatase, osteocalcin, pyridinoline, and deoxypyridinoline). Results: Compared to other groups, elevated bone turnover markers in group 1 were demonstrated (p < 0.05) on the seventh postoperative day. Likewise, compared to other groups, both clinical findings and radiographic union scale significantly improved (p < 0.05) in group 1 on the 56th postoperative day. Conclusions: BM aspirate has a promising beneficial osteogenic effect on mandibular fracture repair in camels, most notably when combined with interdental wire fixation.

Anterior Screw Fixation using Herbert Screw for Type II Odontoid Process Fractures

  • Sung, Joo-Kyung
    • Journal of Korean Neurosurgical Society
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    • v.37 no.5
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    • pp.345-349
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    • 2005
  • Objective: Anterior screw fixation provides the best anatomical and functional results for type II odontoid process fracture (type II-A, II-N, and II-P) with intact transverse ligament. The purpose of this study is to evaluate the usefulness of the 4.5mm diameter, cannulated Herbert screw in anterior screw fixation. Methods: From Jan. 2003 to Feb. 2004, consecutive 10cases of type II odontoid process fractures were treated with anterior screw fixation using a Herbert screw. The Herbert screw has double threads, with different pitches on the distal and proximal ends. It has no head, so it can be inserted through articular cartilage and buried below bone surface. It was originally developed for treating scaphoid fractures. Results: There were 8male and 2female patients whose ages ranged from 15 to 67years (mean 42.1years). The fracture type was type II-A in 4patients, II-N in 3 patients, and II-P in 3 patients. The fracture line was oblique downward and backward in 6cases, oblique downward and forward in 1 case, and horizontal in 3cases. The range of follow-up was 5 to 18months (mean 12months). Bone fusions were achieved in all cases without any instrumental failures or postoperative complications. Conclusion: The Herbert screw is very useful in anterior fixation for type II odontoid process fracture. This series showed successful results also in type II-A odontoid fracture when treated with the Herbert screw, but further more studies are required.

Comparison between Intramedullary Nailing and Percutaneous K-Wire Fixation for Fractures in the Distal Third of the Metacarpal Bone

  • Moon, Sung Jun;Yang, Jae-Won;Roh, Si Young;Lee, Dong Chul;Kim, Jin Soo
    • Archives of Plastic Surgery
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    • v.41 no.6
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    • pp.768-772
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    • 2014
  • Background To compare clinical and radiographic outcomes between intramedullary nail fixation and percutaneous K-wire fixation for fractures in the distal third portion of the metacarpal bone. Methods A single-institutional retrospective review identified 41 consecutive cases of metacarpal fractures between September 2009 and August 2013. Each of the cases met the inclusion criteria for closed, extra-articular fractures of the distal third of the metacarpal bone. The patients were divided by the method of fixation (intramedullary nailing or K-wire). Outcomes were compared for mean and median total active motion of the digit, radiographic parameters, and period until return to work. Complications and symptoms were determined by a questionnaire. Results During the period under review, 41 patients met the inclusion criteria, and the fractures were managed with either intramedullary nailing (n=19) or percutaneous K-wire fixation (n=22). The mean and median total active range of motion and radiographic healing showed no statistically significant difference between the two groups. No union failures were observed in either group. The mean operation time was shorter by an average of 14 minutes for the percutaneous K-wire fixation group. However, the intramedullary nailing group returned to work earlier by an average of 2.3 weeks. Complications were reported only in the K-wire fixation group. Conclusions Intramedullary nailing fixation is advisable for fractures in the distal third of the metacarpal bone. It provides early recovery of the range of motion, an earlier return to work, and lower complication rates, despite potentially requiring a wire removal procedure at the patient's request.

The Posterior Cruciate Ligament (PCL) Reconstruction by the Transtibial Tunnel Method using Cadaveric Achilles Tendon Grafts - Evaluation of the Initial Lengthening and the Slippage Ratio due to the Interference Screw Fixation and Double Cross-Pin Fixation - (사체 아킬레스건을 이용한 경골관통터널방식의 후방십자인대 재건술 - 간섭나사 및 이중고정핀 방식에 따른 초기연신 및 활주율 평가 -)

  • Kim, Cheol-Woong;Bae, Ji-Hoon;Oh, Dong-Joon
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.33 no.4
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    • pp.430-439
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    • 2009
  • Posterior Cruciate Ligament (PCL) plays an important role in knee extension. Rotational instability due to injured PCL can be restored by various PCL reconstruction methods. In this study, the initial lengthening affected by fixation device and location was demonstrated, and furthermore, the slippage and the relationship between lengthening ratio and slippage ratio in the calcaneus and soft tissue fixation methods was newly suggested. Eight specimens of proximal tibia and Achilles tendon grafts were harvested from four cadavers and divided into four groups in regard to the four different types of transtibial fixation techniques. The cyclic load ranged from 50 N to 250 N applied to each graft fixed to proximal tibia in 55 degrees. The initial lengthening ratio to the total elongation has been approximately constant regardless of the fixation methods. The soft tissue fixation method with an interference screw showed about 56.4% slippage ratio to the total elongation and the same method with a double cross-pin presented about 45.4% slippage ratio. The soft tissue fixation method with an interference screw demonstrated approximately 2 mm less total elongation and about 13% more slippage than lengthening because of poor fixation compared to the same method with a double cross-pin.

Comparison between Two Kirschner Wire Fixation and Three Wire Fixation, in Treating of Metacarpal Neck Fracture Using Multiple Retrograde Kirschner Wire Fixation (다발성 역행성 K 강선을 이용한 중수골 경부 골절의 치료에서 2개의 강선과 3개의 강선 사용군의 비교)

  • Kwak, Sang-Ho;Lee, Young Ho;Seo, Gil Joon;Baek, Goo Hyun
    • Journal of Trauma and Injury
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    • v.28 no.2
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    • pp.55-59
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    • 2015
  • Purpose: To compare clinical and radiographic outcomes of between two and three Kirschner wire(K-wire) intramedullary fixation for fractures in the neck of the metacarpal bone. Methods: A single institutional retrospective review identified 28 cases of metacarpal fractures between March 2010 and August 2014. Each of the cases met the inclusion criteria for closed, extra-articular fractures of the neck of the metacarpal bone. The patient groups were divided by the number of K-wire. Outcomes were compared for range of motion of the metacarpophalangeal joint, radiographic parameters, and period until union. Results: The fractures were treated with either 2 Kirschner wire fixation (n=10) or 3 Kirschner wire fixation (n=18). The active range of motion of metacarpophalangeal joint and radiographic result showed no statistically significant difference between the two groups. The mean union period was 5.9 weeks. However, four cases suffered distal head perforation in 2 K-wire fixation group and one case in 3 K-wire fixation group. Conclusion: Multiple retrograde intramedullary Kirschner wire fixation is a good treatment of choice for fractures in the neck of the metacarpal bone. To prevent metacarpal head perforation, it is preferred to use three K-wires than two K-wires.

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Studies on Biological Nitrogen Fixation -III. Influences of organic matter sources, kinds and amount of fertilizer nitrogen on the changes of biological N2-fixation and kjeldahl nitrogen under dark and light condition in submerged paddy soil (생물학적(生物學的) 질소고정(窒素固定)에 관(關)한 연구(硏究) -III. 담수토양(湛水土壤)에서 유기물종류(有機物種類), 질소비종(窒素肥種)과 시비량(施肥量)을 달리했을때 광합성(光合成) 및 타양성질소고정력(他養性窒素固定力)에 미치는 영향(影響))

  • Lee, Sang-Kyu;Lee, Myeong-Gu
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.3
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    • pp.261-268
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    • 1987
  • A green house experiment was conducted to find out the differences in the amount of biologically fixed nitrogen and kjeldahl nitrogen on the different soil texture, kinds and amounts of fertilizer nitrogen under light (photosynthetic $N_2$-fixation) and dark (heterotrophic $N_2$-fixation) condition in submerged paddy soil. The reults obtained were summarized as follows; 1. The amount of biologically fixed nitrogen per mg carbon from different organic matter was obtained as 0.13 mg in glucose, 0.09 mg in rice straw, and 0.07 mg in refused mushroom compost and barley straw under 60 days of incubation. 2. Nitrogen fixing activities were decreased with increase of fertilizer nitrogen and those tendency was pronounced more in sandy soil with application of urea than that of ammonium sulfate. 3. The application of ammonium sulfate in sandy soil under light condition was increased the photosynthetic $N_2$-fixation and the applied urea was remarkably reduced the heterotrophic $N_2$-fixation in sandy soil. The proportion of biologically fixed total nitrogen after experiment in sandy soil was obtained as 25% for dark(heterotrophic $N_2$-fixation) and 75% for light (photosynthetic $N_2$-fixation) condition. On the other hand, very similar biological $N_2$-fixing tendency was obtained between kinds of nitrogen fertilizer and two light condition in clayey soil. 4. The kjeldahl nitrogen was remarkably decreased after experiment under dark condition with application of urea than that of light condition with ammonium sulfate, and no remarkable decreasing tendency was obtained in clayey soil between two kinds of fertilizer nitrogen. 5. The high significant positive correlationship was obtained between calculated biological nitrogen fixation by acetylene reducing activity and kjeldahl nitrogen after experiment under light (y=0.8488X-5.9632, $r=0.9928^{**}$, n=21) and dark (y=0.8795X-7.1056, $r=0.9782^{**}$, n=21) condition. In this experiment condition, conversion factors of 6:1 was obtained from biological nitrogen fixation to soil nitrogen.

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Comparison between Accurate Anatomical Reduction and Unsuccessful Reduction with a Remaining Gap after Open Reduction and Plate Fixation of Midshaft Clavicle Fracture

  • Kim, Joon Yub;Choe, Jung Soo;Chung, Seok Won
    • Clinics in Shoulder and Elbow
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    • v.19 no.1
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    • pp.2-7
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    • 2016
  • Background: The purpose of this study is to compare the radiological and clinical outcomes after open reduction and plate fixation of midshaft clavicle fractures between patients who achieved successful anatomical reduction and those who had a remaining fracture gap even after open reduction and plate fixation, and were thus treated with additional demineralized bone matrix (DBM). Methods: This retrospective analysis was conducted on 56 consecutive patients who underwent open reduction and internal fixation using a locking compression plate for acute displaced midshaft clavicle fractures, and who underwent radiographic and clinical outcome evaluations at least 6 months postoperatively. The outcomes between those who achieved perfect anatomical reduction without remnant gap (n=32) and those who had a remaining fracture gap even after open reduction and plate fixation treated with additional DBM (n=24) were evaluated. Results: There were no differences in the use of lag screws or wiring and operation time (all p>0.05) between those with and without remnant gap. No difference in the average radiological union time and clinical outcomes (satisfaction and Constant score) was observed between the two groups (all p>0.05). However, significantly faster union time was observed for AO type A fracture compared with other types (p=0.012), and traffic accident showed association with worse clinical outcomes compared with other causes of injury. Conclusions: Surgical outcome of midshaft clavicle fracture was more affected by initial fracture type and event, and re-reduction and re-fixation of the fracture to obtain a perfect anatomical reduction spending time appears to be unnecessary if rigid fixation is achieved.

Effect of Organic Fertilizer Levels on Dry Matter Yield, Nitrogen Fixation and Transfer on the Barley and Pea Mixtures (보리.완두 포장에서 유기질비료 시용 수준이 건물수량과 질소 고정 및 이의 이동에 미치는 영향)

  • Lee, Hyo-Won
    • Korean Journal of Organic Agriculture
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    • v.21 no.3
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    • pp.381-389
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    • 2013
  • In order to study the effect of organic fertilizer on dry matter (DM) yield, nitrogen fixation and transfer from pea to barley, an experiment was carried out from May to June in 2008 in Incheon. A completely randomized block design with three replications was used for the experiment and one reference plot assigned each treatment for nitrogen fixation evaluation. Seeding mixture was 40kg barley and 80kg pea per ha. N rate of 40, 80 and 120kg/ha as organic fertilizer was applied at seeding. The equivalent of 1kg per ha as $(15NH_4)_2SO_4$ Solution at 99.8 atom N was applied to the plots ($30{\times}20cm$) at mid April. Forage was harvested from each plot in ripening stage at ground level and separated into barley and pea. Nitrogen fixation was 32.4%, 23.4% and 0% at three different organic N levels. Transfer rate were from 47.6% to 21.8% in difference method and 24.6% and 21.4% on $^{15}N$ dilution method. N Transfer amount were from 92.8kg/ha to 41.9kg/ha on difference method and 47.3kg in the 40kg N plots and 49.7kg in the 80kg N plots on $^{15}N$ dilution method but there was no N transfer in 120kg N organic fertilizer plots. Benefit from increased organic fertilizer was not clear in terms of nitrogen fixation and transfer from pea to barley in barley and pea mixtures.

Short Segment Fixation for Thoracolumbar Burst Fracture Accompanying Osteopenia : A Comparative Study

  • Kim, Hyeun Sung;Kim, Seok Won;Ju, Chang Il;Lee, Sung Myung;Shin, Ho
    • Journal of Korean Neurosurgical Society
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    • v.53 no.1
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    • pp.26-30
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    • 2013
  • Objective : The purpose of this study was to compare the results of three types of short segment screw fixation for thoracolumbar burst fracture accompanying osteopenia. Methods : The records of 70 patients who underwent short segment screw fixation for a thoracolumbar burst fracture accompanying osteopenia (-2.5< mean T score by bone mineral densitometry <-1.0) from January 2005 to January 2008 were reviewed. Patients were divided into three groups based on whether or not bone fusion and bone cement augmentation procedure 1) Group I (n=26) : short segment fixation with posterolateral bone fusion; 2) Group II (n=23) : bone cement augmented short segment fixation with posterolateral bone fusion; 3) Group III (n=21) : bone cement augmented, short segment percutaneous screw fixation without bone fusion. Clinical outcomes were assessed using a visual analogue scale and modified MacNab's criteria. Radiological findings, including kyphotic angle and vertebral height, and procedure-related complications, such as screw loosening or pull-out, were analyzed. Results : No significant difference in radiographic or clinical outcomes was noted between patients managed using the three different techniques at last follow up. However, Group I showed more correction loss of kyphotic deformities and vertebral height loss at final follow-up, and Group I had higher screw loosening and implant failure rates than Group II or III. Conclusion : Bone cement augmented procedure can be an efficient and safe surgical techniques in terms of achieving better outcomes with minimal complications for thoracolumbar burst fracture accompanying osteopenia.

Effects of $NO_3^-$ Gradients on Nitrogen Fixation, Nitrate Reduction and Ureide Content of Soybean (대두의 공소개정, 공산환원 및 Ureide함량에 미치는$NO_3^-$의 영향)

  • 추연식
    • Journal of Plant Biology
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    • v.30 no.3
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    • pp.205-213
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    • 1987
  • Soybean, inoculated with effective Rhizobium japonicum 110, were grown by sand culture with nutrient solution containing either of 0, 1, 3, 10 or 30mM NO3-/l, and analyzed growth characteristics, NR activity, N2-fixation activity, and changes of ureide contents during the growing period. The amount of nodule formation decreased abruptly by nitrate treatment, the maximum nodule dry weight was 1.59, 1.05, 0.78, 0.09 and 0.008 g plant-1, respectively for each treatment on the 98th day. Specfic activity of N2-fixation showed the maximum rates of 140, 101, 37, 5 and 2.2 nM dw.mg-1.hr-1, respectively for each treatment in the earlier growth period. The maximum acetylene reduction activity on the 98th day after sowing was 81.5, 35.3, 14.3, 0.1 and 0.0045 $\mu$M C2H4 plant-1.hr-1, respectively for 0, 1, 3, 10 and 30 mM of NO3- gradients. Nitrate reduction activity increased along with nitrate gradients, and decreased abruptly with age. Relative abundance of ureides in plant organs was high in reproductive growth, and showed the maximum value in fully symbiotic dependent plant. Relative abundance of ureides in stem is a useful indication for the evaluation of nitrogen fixation in nodules of symbiotic plant.

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