• 한국토양비료학회지
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• 제39권2호
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• pp.102-108
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• 2006

비닐피복작물 재배시 시비효율 증대 및 생력시비기술 확립을 목적으로 토중시비기를 개발하였고 2000년 부터 2001년까지 2년 동안 이를 이용하여 양파를 재배한 후 토양개량, 양분이용률 및 수량성에 미치는 영향을 평가하였으며 그 결과는 다음과 같다. 시험 전 토양보다 모든 처리에서 치환성칼륨 함량은 증가되었으나 유효인산 함량은 감소되었다. 생육시기별 토양 중 질산태질소 함량은 모든 처리에서 생육초기에 가장 높았으며 특히 관행시비에 비하여 토중시비에서 $30-60mg\;kg^{-1}$ 높았고 3회 추비에서 생육후기까지 높게 발현되었다. 양파의 생장속도는 토중시비에서 높았고 생육중기 이후 차이가 크게 나타났으나 토중시비 추비횟수 사이에는 유의성이 없었다. 시비질소 흡수량은 관행시비($78kg\;ha^{-1}$)에 비하여 토중시비에서 59-69% 많았고 질소이용률은 추비 2회>추비 1회>추비 3회 순으로 18-20% 높았다. 시비칼리 흡수량은 관생시비($63kg\;ha^{-1}$)에 비하여 토중시비에서 52-71% 많았으며 칼리이용률은 13-17% 높았다. 시비방법별 시비질소 잔존량은 관행시비에 비해 토중시비에서 8-14% 많았고 시비질소 손실량은 관행($154kg\;ha^{-1}$)에 비해 토중시비에서 26-34% 적은 반면에 시비칼리 잔존량은 13-18% 많았고 시비칼리 손실량은 29-31% 적었다. 양파 생육 및 수량은 토중시비에서 엽초경과 구경이 굵고 구중이 무거워 관행시비($45.91Mg\;ha^{-1}$) 대비 7-13% 증수되었으며 질소시비효율이 22-42% 증대되었다.

• 한국초지조사료학회지
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• 제10권2호
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• pp.84-88
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• 1990

This experiment was conducted to evaluate the effect of cutting frequency and nitrogen fertilization on $(NO_3$-N leaching losses under 12 years mixture permanent meadow, The results of this study were summarized as follows: 1. There was no difference in the $(NO_3$-N content of soil water within 1 m soil depth during the whole experimental period. It means that the content of $(NO_3$-N leaching losses was not influenced by botanical composition, cutting frequency and nitrogen fertilization in this experiment. 2. The level of $(NO_3$-N content during the whole experimental period was not in excess of 0.5 ppm level.

• 한국토양비료학회지
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• 제47권5호
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• pp.332-339
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• 2014

This research was performed to test the hypothesis that the optimal fertilization rate for red pepper is changed by soil moisture condition. The experiment was conducted in rainfall-intercepted fields in Suwon, South Korea from 2002 to 2003. Soil was irrigated at 30, 50, or 80 kPa of soil moisture tension at 20 cm soil depth in 2002 and 30, 50, 100, or 150 kPa in 2003. For both years, fertilization was performed with four levels: none, 0.5, 1, and 1.5 times of the recommended N, P, and K fertilization rate. The irrigation amount was the greatest at 30 kPa irrigation while the water use efficiency increased with decrease of irrigation amount. The Irrigation amount was 508 mm at 30 kPa irrigation and ranged from 355 mm to 435 mm at 50 kPa irrigation. The maximum yield was found at 30 kPa irrigation and 1.5 times of the recommend fertilization rate in 2002 and 2003. The yield index of red pepper increased linearly with the fertilization rate at 30 kPa which implied that excess irrigation induced nutrient leaching and reduced nutrient availability. The maximum yield in 50 kPa and 80 kPa was found at the recommend fertilization rate while the yield decreased by fertilization at 100 kPa and 150 kPa irrigation. It implies that reduction of fertilization is the feasible practice to mitigate drought stress in fields without stable irrigation resources.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.491-497
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• 1998

Four types of serum supplements viz. estrus cow serum (ECS), estrus buffalo serum (EBS), pro-estrus buffalo serum (PrBS) and post-estrus buffalo serum (PtBS), added to TCM-199, were evaluated for in vitro maturation and fertilization of buffalo follicular oocytes. The oocytes were recovered from buffalo ovaries after slaughter, using either aspiration or scoring (multiple incisions) method. The recovered oocytes were categorized as A, B and C based on their cumulus investment and ooplasm homogeneity and cultured in four media. The in vitro matured oocytes were inseminated with $1{\times}10^6$ spermatozoa washed in 2.9% sodium citrate solution. The scoring method yielded greater number of morphologically good oocytes than the aspiration method (3.85 vs 1.76 per ovary, p < 0.01). The maturation rates of three categories of oocytes did not differ from one another. The maturation rates of 80.00, 82.08, 78.77 and 66.23%, while the fertilization rates of 54.54, 55.38, 52.80 and 36.76% were recorded for media containing ECS, EBS, PrBS, and PtBS, respectively. The medium containing PtBS gave lower maturation, as well as fertilization, rates than the other three media (p < 0.05). Thus, the scoring method was better than the aspiration method for the recovery of follicular oocytes. The oocytes categorized A, B and C had similar maturation capabilities. The TCM-199 containing buffalo/cow serum collected at pro-estrus or estrus appeared better for in vitro maturation and fertilization of buffalo follicular oocytes than that containing serum collected at post estrus.

• 한국농공학회논문집
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• 제52권3호
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• pp.65-71
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• 2010

This study was conducted to investigate the dynamics of nutrients such as total nitrogen (TN), nitrate nitrogen ($NO_3$-N) total phosphorous (TP), and phosphate phosphorous ($PO_4$-P) in outflow from a cabbage farmland in a mixed land-use watershed. The TN concentrations in groundwater showed twice peaks in late July 2006 and late March 2007 (3.8, 4.7 mg/L, respectively), when it rained shortly after fertilizer application, indicating that nitrogen leaching is greatly influenced by fertilization and rainfall. The mean concentrations of TN and $NO_3$-N in surface water were not significantly higher than those in groundwater, while the mean concentrations of TP and $PO_4$-P in surface water were significantly (p < 0.05) were higher than those in groundwater. The TN concentrations in groundwater were generally higher than those in surface water during fertilization and early growing season due to the effect of fertilization, but vice versa in the other periods. In contrast, the TP concentrations in groundwater were always lower than those in surface water due to the sorption of particulate phosphorous by soil. The ratio of TN load in baseflow to that in total TN load (39 %) was much greater than the TP ratio (7 %), suggesting that baseflow contribute to nitrogen export. Therefore, proper fertilization management should be taken to reduce nitrogen load through baseflow.

• 한국유기농업학회지
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• 제19권spc호
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• pp.182-185
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• 2011

Soil $NH_4$-N content became higher in proportion to the increase in the urea application rate, while in livestock faeces compost (LFC) plots, it became lower than in urea plots and had no significant difference statistically among LFC plots. There was a close relationship between phosphate fertilization rate and the increment of soil available phosphate content after experiment resulting y=0.1788x-6.169 ($R^2=0.9425$) when applied fused superphosphate fertilizer, and y=0.0662x-2.689 ($R^2=0.9315$) when applied LFCs by the same amounts of phosphate (x: phosphate application, kg $ha^{-1}$, y: increment in soil available phosphate content, mg $kg^{-1}$. Plant height, number of stems, nutrients uptake by rice and rice yield showed higher levels in N 100, 150% application plots of chemical fertilizers, while every LFC plots exhibited lower values and no significant difference among them. Relative nitrogen fertilization efficiencies of LFCs compared to urea was 12.3% for cattle faeces compost (CaFC), 8.8 for swine faeces compost (SwFC) and 24.6 for chicken faeces compost (ChFC), respectively.

• 한국토양비료학회지
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• 제27권3호
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• pp.209-214
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• 1994

독활(獨活)에 대(對)한 3요소(要素) 시비량(施肥量) 차이(差異)가 생육(生育) 및 수량반응(收量反應)과 양분흡수(養分吸收)에 미치는 영향(影響)을 구명(究明)하고 적정(適正) 시비량(施肥量)을 얻고자 축행(逐行)과 시험결과(試驗結果)는 다음과 같다. 1. 시비량(施肥量) 증가(增加)에 따라 파종후(播種後) 1년차(年次)와 2년차(年次) 공(共)히 지상부(地上部) 생육(生育)과 뿌리수량(收量)이 증가(增加)되었고 회귀식(回歸式)에 의한 2년차(年次) 3요소(要素) 적정시비량(適正施肥量)턴은 10a당(當) 질소(窒素) 38kg, 인산(燐酸) 21kg, 가리(加里) 18kg이었다. 2. 독활(獨活)의 뿌리수량(收量)에 대(對)한 제형질(諸形質)의 기여도(寄與度) 값을 비교(比較)해 본 결과(結果) 초장(草長), 근경(根徑), 묘두수(苗頭數), 경대(莖大), 근수(根數) 순(順)으로 나타났다. 3. 3요소별(要素別) 상대수량(相對收量) 지수(指數)는 질소(窒素), 가리(加里), 인산(燐酸) 순(順)으로 낮았고, 시비효율(施肥效率)은 가리(加里), 인산(燐酸), 질소(窒素) 순(順)으로 높았다. 4. 독활(獨活) 잎의 무기성분(無機成分) 분석결과(分析結果) 석회함량(石灰含量)이 가장 많은 반면(反面), 인산함량(燐酸含量)이 적게 함유(含有)되어 있고 질소(窒素)와 가리(加里) 시비량(施肥量)이 증가(增加)함에 따라 잎의 질소(窒素)와 가리함량(加里含量)도 증가(增加) 경향이었으며 특히 질소함량(窒素含量)이 뿌리수량(收量)과 고도(高度)의 유의성(有意性) 있는 정의 상관(相關)을 보였다. 5. 무기질비료(無機質肥料)와 유기질비료(有機質肥料) 처리간(處理間)의 생약(生藥) 일반성분함량(一般成分含量) 즉 이물함량(異物含量), 회분(灰分), 에탄올 추출 엑기스를 비교(比較) 분석(分析)한 결과 두 처리(處理)간에 뚜렷한 성분함량(成分含量) 차이(差異)를 인정(認定)할 수 없었다.

• 한국토양비료학회지
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• 제49권2호
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• pp.194-199
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• 2016

In an experiment conducted at the research field of the National Institute of Agricultural Science, we investigated the effects of mineral fertilizer and rice straw compost on exchangeable potassium and K balances, and rice grain yield under a rice single system. The treatments were no fertilization (No fert.), inorganic fertilization (N), inorganic fertilizer (N, P, K) plus rice straw compost at rates of 7.5, 15.0, 22.5, and $30.0ton\;ha^{-1}$ (NPKC7.5, NPKC15.0, NPKC22.5, and NPKC30.0, respectively). The inorganic fertilizers(N, P, K) were added with standard fertilizer application rate in which nitrogen (N), phosphate ($P_2O_5$), and potassium ($K_2O$) were applied with $75{\sim}150kg\;ha^{-1}$, $70{\sim}86kg\;ha^{-1}$, $75{\sim}86kg\;ha^{-1}$, respectively. Exchangeable potassium for NPKC15.0 NPKC22.5, and NPK30.0 treatments was higher by $0.05{\sim}0.19cmol_c\;kg^{-1}$ than that of NPKC7.5 treatment. Increasing levels of rice straw compost resulted in an increase in the K balance from - $19.9kg\;ha^{-1}yr^{-1}$ (No fert.) to $41.9kg\;ha^{-1}yr^{-1}$ at NPKC22.5 treatment and $62.9kg\;ha^{-1}$ at NPKC30.0 treatment. Continuous application of rice straw compost with NPK fertilizers affected significantly the rice grain yields. The result of the study imply that the application of more than $22.5ton\;ha^{-1}$ of rice straw compost with NPK fertilizers are recommended as the best fertilization practice for enhancement of crop production and K supplying power of soil in the continuous rice cropping system.

• 한국양식학회지
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• 제8권3호
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• pp.159-170
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• 1995

참전복의 3배체 유도를 위하여 수정 12분후 및 32분 후에 저온$(0^{\circ}C\;,\;3^{\circ}C)$해수 혹은 고온$(35^{\circ}C\;,\;40^{\circ}C)$ 해수 속에서 여러 처리 지속 시간으로 3배체 유도 실험을 한 결과, 수정율은 실험군 사이에 유의한 차가 없었다. (P>0.05). 그러나 부화율과 정상발생율은 3배체구간 2배체 대조구보다 유의하게 낮았다. (P<0.05). $40^{\circ}C$의 고온처리구에서 수정율은 매우 낮았고$(0\~2.7\%)$ 부화되는 유생은 없었다. 3배체 유도율은 염색체수를 직접 계수해서 구했는데 가장 높은 3배체 유도율은 수정 12분 후에 $3^{\circ}C$ 해수에 15분 동안 처리했을 때 $(84.0\%)$였다. 참전복 2배체의 염색체 수는 2n=36이었고 3배체의 염색체 수는 3n=54이었다.

• Clinical and Experimental Reproductive Medicine
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• 제42권1호
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• pp.22-29
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• 2015

Objective: Sperm must be properly prepared in in vitro fertilization (IVF)-embryo transfer (ET) programs in order to control the fertilization rate and ensure that embryos are of high quality and have appropriate developmental abilities. The objective of this study was to determine the most optimal sperm preparation method for IVF. Methods: Patients less than 40 years of age who participated in a fresh IVF-ET cycle from November 2012 to March 2013 were included in this study. Poor responders with less than three mature oocytes were excluded. Ham's F-10 medium or sperm-washing medium (SWM) was used in combination with the density-gradient centrifugation/swim-up (DGC-SUP) or SUP methods for sperm preparation. A total of 429 fresh IVF-ET cycles were grouped according to the media and methods used for sperm preparation and retrospectively analyzed (DGC-SUP/Ham's F-10, n=82; DGC-SUP/SWM, n=43; SUP/Ham's F-10, n=181; SUP/SWM, n=123). Results: There were no significant differences among these four groups with respect to the mean age of the female partners, duration of infertility, number of previous IVF cycles, and retrieved oocytes. We determined that both the DGC-SUP and SUP methods for sperm preparation from whole semen, using either Ham's F-10 or SWM media, result in comparable clinical outcomes, including fertilization and pregnancy rates. Conclusion: We suggest that both media and both methods for sperm preparation can be used for selecting high-quality sperm for assistive reproductive technology programs.