• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1369-1373
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• 2004

The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at $4^{\circ}C$ with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. However, the sperm motility rapidly declined after 4 day of preservation during incubation. The normal acrosome steadily declined from 93.3% at 0.5 h incubation to 73.8% at 6 h incubation at 1 day of preservation. However, the normal acrosome rapidly declined after 3 day of preservation during incubation. The rates of sperm penetration and polyspermy were higher in 5 and $10{\times}10^6$ sperm/ml than in 0.2 and $1{\times}10^6$ sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in $10{\times}10^6$ sperm/ml compared with other sperm concentrations. The rates of blastocysts from the cleaved oocytes (2-4 cell stage) were highest in $1{\times}10^6$sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at $4^{\circ}C$ could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend $1{\times}10^6$sperm/ml concentration for in vitro fertilization of pig oocytes.

• Clinical and Experimental Reproductive Medicine
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• 제27권2호
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• pp.201-209
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• 2000

Objective: This study has been carried out to evaluation the effect of fertilization promoting peptide (FPP) on the kinematic parameters, capacitation and acrosome reaction of the frozen-thawed human spermatozoa. Methods: After FPP treatment, we examined kinematic parameters, capacitation and acrosome reaction, using the methods of computer-aided sperm analysis (CASA) and chlortetracycline (CTC) fluorescence analysis. Results: We have obtained the evidence that FPP can promote the capacitation and inhibit the spontaneous acrosome reaction of frozen-thawed human spermatozoa in vitro. FPP ($25{\sim}100$ nM) induced a significant increase in the proportion of B-pattem capacitated spermatozoa, and a significant decrease in the proportion of F-pattem uncapacitated ones without significant stimulation of acrosomal exocytosis. In the kinematic parameters treatment, FPP treated groups maint3ined higher LIN, BCF and STR than those of control. The VAP, VSL, VCL and ALH were not different. Therefore it is suggested that FPP in human seminal plasma may play a positive role in promoting human sperm function.

• 한국토양비료학회지
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• 제46권3호
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• pp.173-181
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• 2013

Livestock wastes are considered as major environmental pollutants because they contain high concentration of organic materials. In 2001, The Environmental Department reported that stock farmers were increasing as 5.1%/year, which resulted in a gradual increase in livestock wastes generation. The direct disposal of livestock wastes create several environmental problems. Thus, several countries banned the disposal of livestock wastes in environment including aquatic systems. Recently, aeration-based liquid fertilization was considered as potential way for the disposal of livestock wastes. In this study, next generation sequencing (NGS) analysis was used to understand the microbial community changes during liquid fertilization of livestock wastes. Microbial community was compared with liquid fertilizer physicochemical analysis such as $BOD_5$, $COD_{Mn}$ pH, N (Nitrogen), P (Phosphorus), K (Potassium) etc. The physicochemical parameters and bacterial community results pave the way for producing effective livestock-based fertilizer. By comparing the physical characteristics of the manure with microbial community changes, it is possible to optimize the conditions for producing effective fertilizer.

• 한국토양비료학회지
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• 제48권5호
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• pp.464-468
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• 2015

Our government has performed to support the nation-wide application of customized fertilizer based on soil-testing results and crop nutrient balance in order to promote the environment-friendly agriculture and to respond the global environment guide-line since 2010. This study was performed at the selected local paddy fields (Hwaseong-si, Uiseong-gun and Miryang-si) with different soil chemical properties in 2012. The contents of amino acids measured showed an increasing trend with fertilization, and glutamic acid was the most abundant amino acid followed by aspartic acid, leucine and alanine. However, valine, isoleusine, tyrosine and lysine were not significantly affected by fertilization. The significant differences in grain N, expressed as a crude protein, and amino acids dose was observed between experimental sites (p<0.001), treatments (p<0.01 to 0.001) and interaction of both factors (p<0.01 to 0.001). In our experiment the following order of carbon skeleton backbones to produce amino acids was observed irrespective of experiment sites and fertilization: ${\alpha}$-ketoglutarate > oxalate > pyruvate > 3-phosphoglycerate > phosphoenolpyruvate. In conclusion, customized fertilizer had no difference in amino acids compared to the conventional-NPK practice which was higher than in no fertilization, and also the normal paddy represented slightly higher amino acids compared to the reclaimed. Further study based on the present results is required to investigate what is main factor to amino acids between genetic and environmental factors.

• 한국연초학회지
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• 제17권2호
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• pp.109-113
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• 1995

Nicotiana repanda has resistance to many important tobacco diseases but no tobacco cultivars are currently available that carry risistance genes derived from this species. Numerous attempts to hybridize N, repanda with commercial tobacco cultivars have been largely unsuccessful because of cross incompatibility or the uncovering of lethal genes. In vitro pollination of placenta attached ovules was useful in by passing prezygotic barriers for interspecific hybrid combination between N.tabacum cv. NC82 and N. repanda. Six days after in vitro pollination of N. tabacum cv. NC82×N. repanda, enlarged ovules on plancenta were removed and transferred into ovule culture medium of kitsch and kitsch (1969). Within 15 days of ovule culture, germination occurred. Most of the hybrid seedlings obtained had poor root system and finally died, while few of them had good root system and grew well.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.83-83
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• 2002

This study was to compare the characteristics of parthenogenetic mES (P-mES) cells and in vitro fertilization mES cells. Mouse oocytes were recovered from superovulated 4wks hybrid F1 (C57BL/6xCBA/N) female mice. The oocytes were treated with 7% ethanol for 5 min and 5 ㎍/㎖ cytochalasin-B for 4 h. For IVF, the oocytes were inseminated with epididymal sperm of hybrid Fl male mice (1×10/sup 6//㎖). IVF and parthenogenetic embryos were cultured in M16 medium for 4 days. Cell number count in blastocysts was carried out differential labelling using propidium iodide (red) and bisbenzimide(blue). (omitted)

• 한국연초학회지
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• 제14권2호
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• pp.144-150
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• 1992

This study was carried out to investigate the effect of yield and quality on the harvesting time and methods of stalk cutting in Burley Tobacco.3 harvesting methods and 4 harvesting times of stalk cutting were compared to priming. The yield and quality were high when cut the stalk after second priming in stalk curing. It was also desirable that cut the stalk after second priming in stalk curing. It was also desirable that cut the stalk on 30 days after topping for standard fertilization(N-P2O5-K2O= 17.5-17.5-35.0kg/10a) plot, and 30-35 days after topping for 30% increased fertilization.

• Asian-Australasian Journal of Animal Sciences
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• 제14권9호
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• pp.1342-1351
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• 2001

Over the years, the embryo transfer industry has grown from the simple collection & transfer of embryos into an advanced field of embryo biotechnology. Currently a large demand exists for bovine oocytes and early embryos in both research and commercial settings. Bovine embryos can now be produced in-vitro. Primary oocytes collected from antral follicles of abattoir - obtained ovaries can be induced to undergo the maturation process. In-vitor maturation system, however must ensure that the resulting oocyte is capable of undergoing normal fertilization and yields a zygote competent of developing to term after embryo transfer. Sperm preparation for IVF has improved with the use of heparine. The use of co-culture system has proved beneficial in circumventing the developmental block in IVM/IVF bovine embryos.

• Clinical and Experimental Reproductive Medicine
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• 제23권3호
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• pp.327-331
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• 1996

Normal fertilization and pregnancy by round spermatid was achieved from nonobstructive azoospermia patient to be believed untreatable. Therefore, it is suggested that application of round spermatid in human ART program seems to be new treatment of male infertility. Also, it will be needed further research for evaluated fertilization mechanism by round spermatid injection.

• 한국작물학회지
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• 제46권3호
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• pp.253-259
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• 2001

나지와 작물재배조건의 밭토양에서 질소 공급원으로 질소질 비료 및 완숙 회비를 시비함에 따른 작물체의 생육 및 양분 흡수와 토양내 양분의 이동, 지하수로의 용탈 가능성을 추정하여 시비양분의 행방을 추정하기 위하여 lysimeter에 무비방 임구, 무비무재배 제초구, 무비재배구, 질소 토양처리구(16, 32, 64kg/10a), 질소+퇴비 토양처리구(16+800, 32+1600, 64+3200 kg/10a), 질소 수용액처리구(16, 32kg/10a), 질소보비(32kg/10a)소식 및 밀식구 13 처리로 하여 전작물 옥수수와 후작물 감자의 생육 및 양분의 흡수, 용탈정도를 조사하였다. lysimeter를 통하여 용탈된 물의 양은 나지구(T1, T2)가 작물 재배구들보다 현저하게 많았다. 시비량을 증가시킬수록 전작물(옥수수)과 후작물(감자)의 식물체내 질소함량은 증가하는 경향이었다. 작물체의 생육은 수용액시비구가 토양시비구에 비해 유의하게 양호하였고, 질소흡수율도 수용액시비구가 상대적으로 높았다. 옥수수 및 감자의 건물수량은 질소.퇴비보비구와 증량구가 큰 차이가 없었으나, 식물체내 N, Ca Na, K, Mg의 함량은 시비량이 많을수록 높아지는 것으로 나타났다. 옥수수, 감자의 총질소 함량과 다른 무기성분의 흡수량간에는 Ca, K, Mg과 각각 0.89$^{**}$ , 0.94$^{**}$ , 0.87$^{**}$ 로 매우 높은 상관성을 유지하고 있어 질소의 흡수가 다른 무기성분의 흡수에 영향을 미치고 있음을 보여주었다. 식물체의 실제적인 질소흡수량은 시비량을 증가시킬수록 증가하였으나, 비료로 시용된 질소의 흡수율은 소비구(T4)가 54.9%로 가장 높은 반면, 증량구에서는 31.0-34.0%로 낮아졌다. 토양잔존율은 시비량이 많을수록 높아져 다비구일수록 용탈 가능성이 커지는 것으로 나타났다.