• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.2
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• pp.104-110
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• 1992

This experiment was carried out to determine the effects of nitrogen(N) fertilization levels(0. 120, 240 and 360 kg/ha/y) on seasonal nitrate nitrogen($NO_3$-N) concentration and water soluble carbohydrate (WSC) content of grasses grown under shading condition. The plants were sampled on 7. 14, 21, 28 and 35 days after harvesting in spring(May), summer(July) and autumn(September), 1988. respectively. Shading degree was controlled artificially ca. 45-50%, and each annual level of N was distributed 5 times equally. The $NO_3$-N concentration of grasses was very low(0.064 %) in spring, high in summer(O.l98 %) and autumn(0.234%), and NO,-N was slightly decreased with growing of grasses. Also the NO,-N concentration of grasses was increased significantly with increasing level of N. In spring, the $NO_3$-N of grasses was very safety(less than 0.1 %), regardless of plant height and N level. In summer and autumn, however, plant heights of below 40 cm(summer) and 44 cm(autumn), and N levels of over 210 kg(summer) and 140 kg(autumn) were over toxic level to animals, respectively. The WSC content of grasses was slightly decreased with grass growth. and significantly decreased with high level of N. From the above results, it is suggested that 200 kg/ha of N in this experiment is effective for forage quality. Also a little more amount of N(50-70kgltimes) in spring. and low level of N(20-30 kgltimes) in summer and autumn season may be desirable on woodland posture.

• Clinical and Experimental Reproductive Medicine
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• v.44 no.2
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• pp.63-72
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• 2017

Objective: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry. Methods: For profiling of the total proteome and glycoproteome, pooled protein samples from natural and stimulated hFF samples were selectively isolated using hydrazide chemistry to obtain the total proteomes and glycoproteomes. N-GPs were validated by the consensus sequence N-X-S/T (92.2% specificity for the N-glycomotif at p<0.05). All data were compared between natural versus hyperstimulated hFF samples. Results: We detected 41 and 44 N-GPs in the natural and stimulated hFF samples, respectively. Importantly, we identified 11 N-GPs with greater than two-fold upregulation in stimulated hFF samples compared to natural hFF samples. We also validated the novel N-GPs thyroxine-binding globulin, vitamin D-binding protein, and complement proteins C3 and C9. Conclusion: We identified and classified N-GPs in hFF to improve our understanding of follicular physiology in patients requiring assisted reproduction. Our results provided important insights into the prevention of hyperstimulation side effects, such as OHSS.

• Journal of Korean Society of Forest Science
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• v.106 no.1
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• pp.19-25
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• 2017

This study was conducted to investigate the effects of nitrogen fertilization on physiological characteristics and growth of Populus sibirica seedlings in a semi-arid area, Mongolia. 2-year-old P. sibirica seedlings were planted in May, 2015 with applications of urea 5 g (N1), 15 g (N2), 30 g (N3) and ammonium sulfate 33 g (NS; same nitrogen amount with N2) to each seedling. Chlorophyll contents were significantly different among treatments in August, but not in June and July. The lower chlorophyll contents in August than those in June and July might be related to leaf senescence. In June and July, net photosynthetic rate was higher in NS and N2 than in the control. Unlike the tendency of photosynthesis, transpiration rate was highest in N2, but lower in NS than in any other nitrogen treatments. Relative growth rate of root collar diameter was significantly higher only in NS than in the control and it of height did not differ among treatments. Leaf area in nitrogen treatments was not significantly different from that in the control. Ammonium sulfate seemed to be more suitable fertilizer than urea for the early growth of P. sibirica seedlings in the study site. However, as the effects of urea and ammonium sulfate on soils and seedlings were different, further studies would be necessary to determine the optimal amount of ammonium sulfate.

• Journal of Soil and Groundwater Environment
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• v.6 no.3
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• pp.3-12
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• 2001

This study conducted to Investigate the movement of fertilized nutrients in a volcanic ash soil in Jeju using by the pressure-vacuum soil water sampler. The percolated water to measure the ion concentration of leachates was taken from a lysimeter at depths of 20, 40, 60, 80, 100 and 120 cm in the soil in where a corn and potato were cultivated as a preceding and succeeding crop, respectively. Fertilizers of N-$P_2$O$_{5}$-$K_2$O were applied at the rate of 36-30-30 kg $10a^{-1}$ for the corn and 28-22-24 kg $10a^{-1}$ for the potato prior to planting of both crops. The highest concentrations of Cl , $NO_3$-N, $Ca^{+2}$ and $K^+$ in percolates were showed at 20cm and 40cm in soil depth at one month after fertilizing, and then gradually moved and reduced into below soil depths. At 5.5 months after fertilization, the concentrations in all soil depths were similar with the value of before fertilization. At depth of 120cm, the concentration of NO$_3$-N and the other cations in leachate was highest 1 to 1.5 months after fertilization. pH in percolated water was negatively correlated with NO$_3$-N concentration while the concentration of $NO_3$-N showed positive correlation between Cl, $Ca^{+2}$ and $Mg^{+2}$ concentrations. This result indicated that those cations can be leached out by accompanied with $NO_3$-N.

• Korean Journal of Organic Agriculture
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• v.2 no.1
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• pp.2-15
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• 1993

Major vegetables and fruits in Korea are grouped in 3 Categories according to the Concentration of NO3- content in edible parts of the plant. NO3- content are high in order fruit

• Korean Journal of Environmental Agriculture
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• v.23 no.1
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• pp.7-14
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• 2004

This study was conducted to investigate the effect on agricultural environment and crop productivity by different amount of applied fertilizer in consideration of irrigation water quality. N, P and K contents of irrigation water used in this experiment were 6.16, 0.26 and 9.37 mg/L, respectively. N, P and K Concentrations of runoff water were lower than those of inflow water during rice cultivation. N, P and K Concentrations of ponded and percolated water were changed according to the amount and time of applied fertilization. During rice cultivation in paddy soil, nitrogen balance was closed to 0 in SFT 50% (50% level of soil testing fertilization), 0.14 kg/ha, but it was 95.3 kg/ha in CF (conventional fertilization) treatment In SW 50% and STF (soil testing fertilization) treatment yield of perfect rice was not greatly different as compared with CF treatment due to the superiority of ripening rate, 1,000 grains weight and milling characteristics. Mechanical paratability of rice was excellent in NF (non fertilization) treatment, STF 50% treatment showed higher in nutrient availability and fertilizers use efficiency than other treatments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.1
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• pp.8-14
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• 2013

The newly developed fertilizer is the slow-releasing fertilizer which can be used as a basal fertilizer without no additional fertilization at tillering stage. It has 30-4-6% of $N-P_2O_5-K_2O$ and was coated with mixture of LDPE (Low density polyethylene), EVA (Ethylene vinyl acetate), BDP (Bio degraded polymer), TALC and nonionic surfactant for the controlled release up to 50 days after application. Coating materials were designed to be decomposed naturally. This fertilizer can be applied directly to the seedling tray mechanically just before transplanting, resulting in significant labor saving effect. The developed slow-release fertilizer, which can replace both basal fertilization and top dressing at tillering stage by single application directly to seedling tray, showed the highest release at 14~21 days after transplanting. Considering the plant growth at different growth stages and yield, the optimal application rate of developed slow-release fertilizer was 300 g per rice nursery tray and the yield of rice at this application rate was 5.25 MT/ha. Rice quality in terms of head rice grain ratio, amylose content, whiteness, and taste value decreased as fertilization rate increased from 200 g to 500 g per nursery tray. Fertilization rate based on quantity of fertilizer ingredients (N, P, K) was reduced by 49.3% compared to the standard application rate and there was 49.2% reduction in labor input for fertilization.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.2
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• pp.134-140
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• 2021

This study was carried out to study the effect of supplementary nitrogen fertilization application time according to regrowth date on growth date on growth characteristics, feed value, and productivity of Italian ryegrass (Lolium multiflorum Lam.; IRG) from 2019 to 2021 in Cheonan region. In order to determine the regrowth time, IRG was cut from experimental plots 3cm above soil level and the time when 1cm grew was judged as the regrowth time. The regrowth dates were February 15^th and February 12th in 2020 and 2021 respectively. The experimental design was a randomized block design with three replications. The treatments were no fertilizer, immediately after the regrowth period, after 10 days of the regrowth period, and after 25 days of the regrowth period. Dry matter yield of IRG was significantly influenced by the supplementary N-fertilization application time. Dry matter yield was reduced for the delayed application time. The dry matter yield of immediately after the regrowth period was approximately 34.8 % higher than that no fertilizer application. In delaying the supplementary N-fertilization application time resulted in increased crude protein content. However, no significant statistical difference was neutral detergent fiber and acid detergent fiber(p>0.05). After wintering, productivity of IRG reduced for the delayed application time. Therefore, it is essential to application N-fertilizer immediately after the regrowth period for high productivity of IRG.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.136-141
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• 2013

Lowering tree height has been a key practice for efficient management of persimmon orchards. This experiment was conducted to assess whether fertilization rates could be reduced after lowering the tree height by heavy pruning. Twelve-year-old 'Uenishiwase' persimmon trees were lowered to 2 m from over 3 m by severe dormant pruning, and then conventional fertilization rate was applied to some trees in April (N 224 g, $P_2O_5$ 172 g, and $K_2O$ 172 g per tree), June (N 112 g and $K_2O$ 99 g per tree), and October (N 112 g and $K_2O$ 99 g per tree). At the same time, 1/3 or 2/3 of the conventional rate and none were applied to other trees, respectively. Non-application decreased shoot length and reduced number of unnecessary secondary shoots by 39% compared with the conventional rate, not affecting yield and weight, color, firmness, and soluble solids of fruits. No significant difference was also found in the yield and the fruit characteristics among the trees fertilized with different rates. Concentrations of soluble sugars, starch, N, and K of dormant shoots in March of the following year were not significantly changed by the different treatments of the previous year. There was no significant difference of shoot growth and yield among the treatments the following year when the same fertilization rate was supplied to all the trees. Results indicated that fertilization rate could be reduced to less than 1/3 of conventional rate to save the cost and stabilize shoot vigor when tree height is lowered by severe pruning.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.3
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• pp.169-178
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• 2003

This pot experiment was conducted to find out the effects of boron application($B_{0}$ ; control, $B_1$; 0.2, $B_2$; 2.0, B$_3$; 6.0, $B_4$; 15.0 boron me/pot) on the forage performance of pure and mixed cultures of orchardgrass and white clover. The third part was concerned with the changes in the contents and yields of nitrogen compounds(crude/pure protein and soluble N-compound) in forages. The results obtained are summarized as follows: 1. With no additional fertilization, especially nitrogen, in a pure culture, the $B_{0}$ and $B_4$ treatments on white clover decreased the amount of crude/pure protein, and showed nitrogen deficiency symptoms. However, the optimum boron application($B_2$) positively resulted in the increase of crude and pure protein, especially pure protein, and the content ratio of pure protein/soluble N-compounds. With additional fertilization, especially nitrogen, differences were not found among the boron treatments($B_{0}$, $B_2$, and $B_4$). 2. Owing to the decline of white clover as affected by the additional fertilization, especially nitrogen, in the grass-clover mixed cultures, the effects of B-application on these contents of white clover were different and relatively low, compared with the pure cultures. But the positive effect of $B_2$ treatment tended to be similar to the pure cultures. Also, it was recognized that the $B_2$ treatment resulted in the increase of their contents in orchardgrass, however, the effect was relatively minor compared with that of white clover. 3. The optimum B application(B$_2$) on white clover influenced relatively better on the yields of crude and pure protein than on the dry matter yields, especially with no additional fertilization. The effects of boron application on the contents and yields of crude and pure protein were different according to the forage species, whether it was a pure or mixed culture, and additional fertilization.