• Korean Journal of Environmental Agriculture
• /
• v.25 no.1
• /
• pp.25-33
• /
• 2006

Objectives of this experiment were to select of the optimum water plant and to investigate the removal efficiency of the major nutrients in the constructed wetland by natural purification method for municipal sewage treatment in the farming and fishing village. For the experiment we used "constructed wetland" which was consisted of aerobic (vertical filter system) and anaerobic systems (horizontal filter system). Both systems were filled with gravel and filter media, and grew water plants on top of them. And then, we investigated several items such as sewage treatment efficiency, growth status of water plants and the absorbed amount of inorganic element in water plants with periodical periods. In aerobic area, removal efficiencies of BOD, COD, T-N and T-P were over 92%, 74%, 25% and 57%, respectively, and then when the water is passed through anaerobic area, the efficiency was over 96%, 84%, 44% and 71%, respectively, which was increased more treatment efficiency than that of aerobic area. Absorption amount of nitrogen and phosphorous in Miscanthus sacchariflorus BENTH were the highest in the water plants as 17.7 and 2.41 g/plant in the aerobic area, respectively. Absorption amounts of nitrogen and phosphorous in Scirpus tabernaemontani GMEL were the highest in the water plants as 8.7 and 1.13 g/plant in the anaerobic area, respectively. For the selection of optimum water plants in the constructed wetland by natural purification method for municipal sewage treatment, it were observed that there were Miscanthus sacchariflorus BENTH, Phragmites japonica STEUD and Phragmites communis TRINIUS in the aerobic area and were Zizania latifolia TURCZ, Scirpus tabernaemontani GMEL, Typha orientalis PRESL, Iris pseudoacorus L and Cares dispalata BOOTT in the anaerobic area.

• Journal of the Korean Chemical Society
• /
• v.5 no.1
• /
• pp.33-37
• /
• 1961

We have shown that carboxy-peptidase destroys the biological activity of angiotensin octa-and deca-peptides. Since Proline occurs as the seventh amino acid from the amino end of the chain and since carboxypeptidase does not cleave proline from a peptid chain, it is evident that the heptapeptid H.asp-arg-val-tyr-ileu-his-pro.OH is formed by this hydrolysis. This peptide must then be biologically inactive. In order to determine whether the phenyl group of the C-terminal amino acid was the necessary requirement for biological activity of the octapeptide, $ala^8$ angiotensin octapeptide(amino acids of peptides numbered from amino end) was synthesized. For this synthesis the four dipeptides were prepared: carbobenzoxy-L-prolyl-L-alanine-P-nitrobenzyl-ester, m.p. $134-135^{\circ}C,$ carbobenzoxy-L-isoleucyl-imidazole benzyl-L-histidine methyl ester, m.p. $114-116^{\circ}C,$ carbobenzoxy-L-valyl-L-tyrosine hydrazide and carbobenzoxy B-benzyl-L-aspartyl-nitro-L-arginine. The first three dipeptides were obtained as crystalline compounds. Imidazole-benzyl-L-histidine was used in the hope that it would block the histidine imidazole against side reactions in steps subsequent to the formation of the C-terminal tetrapeptide. Also, it was through that the imidazole benzylated peptides would be easier to crystallize. This, however, was not the case. The tetrapeptide, carbobenzoxy-L-isoleucyl-L-im, benzyl-histidyl, L-prolyl-L-alanine-nitrobenzyl ester was not obtained in a crystalline form. Neither could the mono-or dihydrobromide of the tetrapeptide free base be induced to crystallize. Carbobenzoxy-L-valyl-L-tyrosine azide was condensed with the tetrapeptide free base to yield the protected hexapeptide; carbobenzoxy-L-valyl-L-tyrosyl-L-isoleucyl-L-im, benzyl, histidyl-L-Prolyl-L-alanine-nitrobenzyl ester. Upon removal of the carbobenzoxy group with hydrogen bromide in acetic acid an amorphous free base hexapeptide ester was obtained. This compound gave the correct C, H, N analysis and contained the six amino acids in the correct ratio. The octapeptide was obtained by condensing this hexapeptide with carbobenzoxy-B-benzyl-L-aspartyl-nitro, L-arginine using the mixed anhydride method of condensation. This amorphous product was proven to be homogenous by chromatography in two solvent systems and upon hydrolysis yielded the eight amino acids in correct ratio. The five protecting groups were removed from the octapeptide by hydrogenolysis over palladium black catalyst. Biological assay of the free peptide indicated that it possessed less than 0.1 per cent of both pressor and oxytocic activity of the phenylalanine8 angiotensin. This suggests that the phenyl group is a point of attachment between angiotensin and its biological receptor site.

• Journal of Embryo Transfer
• /
• v.21 no.3
• /
• pp.191-198
• /
• 2006

The objectives of this study was to produce valuable offsprings of Hanwoo and Jeju black cattle using in vivo embryo production and embryo transfer techniques during 5 years ($2001{\sim}2005$) in Jeju. Two hundred and eighty six Hanwoo and sixty nine Jeju black cattles, at random stages of the estrous cycle, received a CIDR. Seven days later, the animals were superovulated with a total of 400 mg pFSH ($Folltropin^{(R)}-V$) administered twice daily in constant doses (each 50 mg) over 4 days. On the 6th administration of FSH, CIDR was withdrawn and 25 mg $PGF_{2{\alpha}}$ was administered. Cows were artificially inseminated thrice after estrous detection at 12 hr intervals. The cows received $250{\mu}g$ GnRH at the time of 2nd insemination. Embryos were recovered 7 or 8 days after the 1st insemination. Recipients were synchronized with donors by insertion of a CIDR for 7 days and administration of 25 mg $PGF_{2{\alpha}}$ at the time of CIDR removal. The collected embryos were transferred to 1,219 recipients by 6 transfer persons. The mean numbers of total ova and transferable embryos from Hanwoo and Jeju black cattle donors were 7.4 and 4.7, respectively The number of transferable embryos differed between Hanwoo (5.0) and Jeju black cattle (3.5, p<0.05), while that of total ova did not differ. Repeated superovulation treatments decreased (p<0.05) the ratio of numbers of the flushed animals vs. superovulated animals in Jeju black cattle, and the numbers of total ova and transferable embryos in Hanwoo. More transferable embryos were collected at summer (5.6) than winter (2.9, p<0.01). The mean pregnancy rate was 40%. The pregnancy.ate was affected by transfer year (2001<2004, p<0.05) and transfer person ($33.0{\sim}41.9%$, p<0.01), while not by donor (embryo) breed. These results showed that in vivo embryo preduction and embryo transfer techniques using CIDR for Hanwoo and Jeju black cattle donors as well as recipient, regardless of their estrous cycle, may enable a stable embryo production and recipient preparation.

• Tuberculosis and Respiratory Diseases
• /
• v.53 no.4
• /
• pp.431-438
• /
• 2002

Background : In order to investigate the usefulness and safety of Natural Stent, we performed this study in a canine model of tracheal stenosis induced using Nd-YAG laser. Materials and Methods : After tracheal stenosis was induced in 12 Mongrel dogs using Nd-YAG laser, either Dumon (n=6) or Natural (n=6) stent was inserted into the trachea. To assess the degree of stent migration and mucostasis, bronchoscopy was performed every week for 4 weeks, after which all stents were removed. One week after stent removal, tracheal stenosis was evaluated by bronchoscopy. Results : The degree of stent migration was not different between the dogs with Dumon stent ($3.0{\pm}0.8$) and those with Natural ($2.0{\pm}1.0$), nor was the degree of mucostasis, at Dumon ($1.7{\pm}0.5$) and Natural Stent ($1.5{\pm}0.6$), respectively. One week after stent removal, the degree of tracheal stenosis was not different between the Dumon ($1.5{\pm}0.5$) and the Natural group ($1.0{\pm}0.4$). In addition, there was no death and the degree of tracheal stenosis remained always within the safe limit (less than 2.0) in all animals. Conclusion : In a canine model of tracheal stenosis induced using Nd-YAG laser, the usefulness and safety of Natural Stent were similar to those of Dumon Stent. A clinical trial is necessary to document the usefulness and safety of Natural Stent in patients with tracheal stenosis.

• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.2
• /
• pp.317-324
• /
• 2012

To reduce non-point source pollutants in Juam Lake eco-wetlands, purification efficiencies of pollutants were investigated at three different systems. The constructed wetlands (CWs) consisted of A system, B system and C system. A system consisted of $1^{st}$ free water surface (FWS) CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ subsurface flow (SSF) CW and $5^{th}$ SSF CW. B system consisted of $1^{st}$ FWS CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ FWS CW and $5^{th}$ SSF CW. C system consisted of $1^{st}$ FWS CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ FWS CW and $5^{th}$ SSF CW. The concentrations of BOD, COD, SS, T-N and T-P in inflow ranged 0.20 ~ 0.91, 1.24 ~ 8.00, 0.60 ~ 8.60, 0.04 ~ 2.50 and $0.001{\sim}0.685mg\;L^{-1}$ from March to October in 2011, respectively. Removal rates of BOD, SS, T-N and T-P were high in Autumn, Spring, Spring and Summer, respectively. In A system, $1^{st}$ FWS CW, $2^{nd}$ FWS CW and $3^{rd}$ FWS CW were dominated by Leersia oryzoides. In B system, $1^{st}$ FWS CW, $3^{rd}$ FWS CW and $4^{th}$ FWS CW were dominated by Leersia oryzoides. In C system, $2^{nd}$ FWS CW and $3^{rd}$ FWS CW were dominated by Nymphaea teragona.

• Journal of Korean Society of Water and Wastewater
• /
• v.26 no.5
• /
• pp.619-627
• /
• 2012

In order to determine reduction of greenhouse gas emissions (GHGs) when the submerged membrane bioreactor with granular sulfur (MBR-GS) is used in wastewater treatment plant (WTP), the amount of GHGs was compared and analyzed in the advanced treatment process of P wastewater treatment plant (WTP). The amount of GHGs was estimated by classifying as construction and operation phase in WTP. The amount of GHGs in construction phase was evaluated from multiplying raw materials by using carbon emission factors. Also the amount of GHGs in operating phase was calculated by using total electricity consumption and carbon emission factor. The construction of anoxic tank and secondary settling tank is unnecessary, because the MBR-GS conducts simultaneously the nitrification and denitrification in aeration tank and filtration by hollow fiber membrane. The amount of $CO_2$, $CH_4$, and $N_2O$ emitted by constructing the MBR-GS was 6.44E+06 kg, 8.16E+03 kg and 1.38E+01 kg, respectively. The result shows that the GHGs was reduced about 47 % as compared with the construction in the MLE process. In operating the MBR-GS, the electricity is not required in the biological reactor and secondary setting tank. Thus, the amount of $CO_2$, $CH_4$, and $N_2O$ emitted by operating in the MBR-GS was 7.39E+05 kg/yr, 5.80E+02 kg/yr and 2.44E+00 kg/yr, respectively. The result shows that the GHGs were reduced about 37 % as compared with the operation in the MLE process. Also, $LCCO_2$(Life Cycle $CO_2$) was compared and analyzed between MLE process and MBR-GS. The amount of $LCCO_2 $emitted from the MLE process and MBR-GS was 3.56E+04 ton $CO_2$ and 2.12E+04 ton $CO_2$, respectively. The result shows that the GHGs in MBR-GS were reduced to about 40 % as compared in the MLE process during life cycle. As a result, sulfur-utilizing autotrophic denitrification process (SADP) is expected to be utilized as the cost-effective advanced treatment process, owing to not only high nitrogen removal efficiency but also the GHGs reduction in construction and operation stage.

• Korean Journal of Ecology and Environment
• /
• v.47 no.spc
• /
• pp.48-56
• /
• 2014

Weekly changes of water environments and phytoplankton community with the salinity gradients were investigated at Gyoungpo Lake from April to November in 1998 and 2012. Underwater crossam in Gyoungpo Lake was removed in 2004. Thereafter, average salinity of Gyoungpo lake increased from 7.5 ppt in 1998 to 20 ppt in 2012. A total of 99 and 80 species of phytoplankton was observed from the sampled in 1998 and 2012, respectively. The number of common species during the 2 separate years was 40. Transparency, SS, $NO_3-N$ concentration and N/P ratio in 2012 were lower than those in 1998. During the period of water shortage (April, May) of 2012 transparency decreased due to decreased salinity and increased SS and Chl. a. Correlation coefficients between species and community scores of DCA ordination based on data matrix of the phytoplankton revealed larger variation among sampling seasons in 1998 than in 2012. The increase of seawater influx and conversion rates following the removal of the underwater crossbeam might explain such a differential variation. Gymnodium sp., Peridinium sp., Prorocentrum sp., Nitzschia longissima, Schroederia setigera, Lyngbya sp., Asterococcus limneticus, Asterococcus superbus and Cyclotella meneghiniana were found to well adapt at the high salinities in 2012. Comparatively, Asterrionella formosa, Nitzschia frustulum, Chlorella ellipsoidea, Scenedesmus bijuga and Scenedesmus ellipsoideus were observed at lower salinities in 1998. Two quite contrasting phytoplankton communities were found in the two seasons of a year, spring with limited precipitation and summer, the flood season.

• Journal of the Korean GEO-environmental Society
• /
• v.13 no.5
• /
• pp.5-12
• /
• 2012

Antibiotics have been considered emerging compounds due to their continuous input and persistence in environment. Due to the limited biodegradability and widespread use of these antibiotics, an incomplete removal is attained in conventional wastewater treatment plants and relative large quantities are released into the environment. In this study, it was determined the adsorption and photocatalysis kinetics of antibiotics (Sulfamethoxazole, SMX) with various catalyst (Titanium dioxide; $TiO_2$, Hydroxyapatite; HAP) conditions under UV/$TiO_2$/HAP system. In addition, the statistical analysis of response surface methods (RSM) was used to determine the effects of operating parameters on UV/$TiO_2$/HAP system. $TiO_2$/HAP adsorbent were found to follow the pseudo second order reaction in the adsorption. In the result of applied intrapaticle diffusion model, the constants of reaction rate were $TiO_2$=$0.064min^{-1}$, HAP=$0.2866min^{-1}$ and $TiO_2$/HAP=$0.3708min^{-1}$, respectively.The result of RSM, term of regression analysis in analysis of variance (ANOVA) showed significantly p-value (p<0.05) and high coefficients for determination values($R^2$=96.2%, $R^2_{Adj}$=89.3%) that allowed satisfactory prediction of second order regression model. And the estimated optimal conditions for Y(Sulfamethoxazole removal efficiency, %) were $x_1$(initial concentration of Sulfamethoxazole)=-0.7828, $x_2$(amount of catalyst)=0.9974 and $x_3$(reation time)=0.5738 by coded parameters, respectively. According to the result of intraparticle diffusion model and photocatalysis experiments, it was shown that the $TiO_2$/HAP was more effective system than conventional AOPs(advanced oxidation processes, UV/$TiO_2$ system).

• Korean Journal of Environmental Agriculture
• /
• v.30 no.3
• /
• pp.304-309
• /
• 2011

BACKGROUND: Generally, water plants may play an important role in nutrients(N, P) removal in constructed wetlands(CWs). Previous studies considered nutrients uptake by water plants in various CWs for treating point source pollution. On the other hand, few studies considered nutrients uptake by water plants in free water surface(FWS) CWs for treating non-point source pollution. METHODS AND RESULTS: To investigate characteristics of nutrient uptake by water plants in FWS CWs, dry weights, nutrients content and nutrients uptake by water plants were investigated from April, 2008 to October, 2008. Dominance plants were Phragmites japonica STEUD (PHRJA), Nymphaea tetragona ANGUSTA(NTMTE), Typha orientalis PRESL(TYHOR), Phragmites communis TRINIUS(PHRCO) and Zizanis latifolia TURCZ(ZIZLA) in FWS CWs. The dry weights of water plants in August were higher in the order of TYHOR(54.27 g/plant) > PHRJA(44.30 g/plant) ${\geqq}$ PHRCO(39.60 g/plant) ${\geqq}$ ZIZLA(37.80 g/plant) ${\fallingdotseq}$ NTMTE(36.75 g/plant). The T-N and T-P contents by water plants were not significantly differences regardless of cultivation period. The maximum amount of T-N uptake by water plants in August were 773 mg/plant for PHRJA, 625 mg/plant for NTMTE, 1206 mg/plant for TYHOR, 754 mg/plant for PHRCO and 768 mg/plant for ZIZLA. The maximum amounts of T-P uptake by PHRJA, NTMTE, TYHOR, PHRCO and ZIZLA were 397, 177, 411, 261 and 229 mg/plant in August, respectively. CONCLUSION(s): The results of this study suggest that optimum water plant was Typha orientalis PRESL in free water surface constructed wetlands.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.20 no.1
• /
• pp.61-70
• /
• 2012

Feasibility of co-digestion was investigated by a series of anaerobic batch experiments using sewage sludge, swine waste, and food waste leachate as substrates. The organic solid wastes were collected from M city, where the daily productions of sewage sludge, swine waste, and food waste leachate were 178 ton/d, 150 ton/d, and 8 ton/d, respectively. Both swine waste and food waste leachate showed superior methane yields, methane productivities, and organic pollutant removal efficiencies compared to sewage sludge. Co-digestion of the total amounts of organic solid wastes would enhance methane production by 5.60 times $(530\;m^{3}\;CH_{4}/d\;{\rightarrow}\;2,968\;m^{3}\;CH_{4}/d)$. However, it also increase the amount of digestate by 1.88 times with 3.79 to 4.92 times higher pollutants (chemical oxygen demands total nitrogen, and total phosphorus) loading rates. Co-digestion of organic solid wastes is a valid strategy to enhance the performance of an anaerobic sludge digester and the energy independence of a wastewater treatment plant. Anyhow,the increment of digestate with higher pollutant loading would need a careful counterplan in the operation of the main stream of the treatment plant.