Dongheon Lee;Hye-Jin Kim;Azfar Ismail;Sung-Su Kim;Dong-Gyun Yim;Cheorun Jo
Animal Bioscience
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v.36
no.7
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pp.1101-1119
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2023
Objective: This study aimed to evaluate the physicochemical, metabolomic, and sensory qualities of Chikso and Hanwoo beef during 28 days of wet aging. Methods: Rump and loins from Hanwoo and Chikso were obtained and wet-aged for 28 days at 4℃. The samples were collected at 7-day interval (n = 3 for each period). Physicochemical qualities including pH, meat color, shear force value, and myofibrillar fragmentation index, metabolomic profiles, and sensory attributes (volatile organic compounds and relative taste intensities) were measured. Results: Chikso showed a significantly higher shear force value than Hanwoo on day 0; however, no differences between breeds were found after day 14, regardless of the cuts. Overall, Chikso had more abundant metabolites than Hanwoo, especially L-carnitine and tyrosine. Among the volatiles, the ketone ratio was higher in the Chikso rump than the Hanwoo rump; however, Chikso had fewer alcohols and aldehydes than Hanwoo. Chikso rump showed higher taste intensities than the Hanwoo rump on day 0, and sourness decreased in Chikso, but increased in the Hanwoo rump on day 14. Wet aging for 14 days intensified the taste of Chikso loin but reduced the umami intensity of Hanwoo loin. Conclusion: Chikso had different metabolomic and sensory characteristics compared to Hanwoo cattle, and 14 days of wet aging could improve its tenderness and flavor traits.
Objectives: In this preliminary study, we investigated the clinical characteristics of patients who were first diagnosed with psychotic disorder and explored the impact of the adherence to antipsychotics on long-term medical use. Methods: All national health insurance claims related to psychotic disorders including gender, age, income, and drug compliance, from January 1, 2008 to December 31, 2015, were examined. With trend test using Medication Possession Ratio (MPR), we compared the medical use between the compliant group (MRP≥0.8) and the comparative non-compliant group (0.2≤MPR<0.8). Results: Among 28,095 participants in total, 16,239 patients (57.8%) were diagnosed as schizophrenia; the 30s were the most common (n=7,151, 25.5%). Drug compliance was generally low regardless of the diagnosis and was the lowest among 20s with the 40-60% range of income. The compliant group showed lower psychiatric and medical use than the comparative group in the following years (p<0.0001). Conclusion: These findings suggest that patients in the 20s and 30s with the 40-60% range of income, who are diagnosed with schizophrenia at the first psychiatric visit, may need more clinical and political attention. The results also emphasize the importance of initial drug adherence to antipsychotics in reducing long-term psychiatric costs.
N. Nithiyanandam;C. Mahesh;S.P. Raja;S. Jeyapriyanga;T. Selva Banu Priya
KSII Transactions on Internet and Information Systems (TIIS)
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v.17
no.6
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pp.1706-1727
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2023
Under Water Sensor Networks (UWSN) has gained attraction among various communities for its potential applications like acoustic monitoring, 3D mapping, tsunami detection, oil spill monitoring, and target tracking. Unlike terrestrial sensor networks, it performs an acoustic mode of communication to carry out collaborative tasks. Typically, surface sink nodes are deployed for aggregating acoustic phenomena collected from the underwater sensors through the multi-hop path. In this context, UWSN is constrained by factors such as lower bandwidth, high propagation delay, and limited battery power. Also, the vulnerabilities to compromise the aquatic environment are in growing numbers. The paper proposes an Energy-Efficient standalone Intrusion Detection System (EEIDS) to entail the acoustic environment against malicious attacks and improve the network lifetime. In EEIDS, attributes such as node ID, residual energy, and depth value are verified for forwarding the data packets in a secured path and stabilizing the nodes' energy levels. Initially, for each node, three agents are modeled to perform the assigned responsibilities. For instance, ID agent verifies the node's authentication of the node, EN agent checks for the residual energy of the node, and D agent substantiates the depth value of each node. Next, the classification of normal and malevolent nodes is performed by determining the score for each node. Furthermore, the proposed system utilizes the sheep-flock heredity algorithm to validate the input attributes using the optimized probability values stored in the training dataset. This assists in finding out the best-fit motes in the UWSN. Significantly, the proposed system detects and isolates the malicious nodes with tampered credentials and nodes with lower residual energy in minimal time. The parameters such as the time taken for malicious node detection, network lifetime, energy consumption, and delivery ratio are investigated using simulation tools. Comparison results show that the proposed EEIDS outperforms the existing acoustic security systems.
Background: This study analyzed the physiological adaptations of a woody plant, Vitex rotundifolia, in Goraebul coastal sand dunes from May to September 2022. Environmental factors and physiological of plants growing under field and controlled (pot) conditions were compared. Results: Photosynthesis in plants growing in the coastal sand dunes and pots was the highest in June 2022 and July 2022, respectively. Chlorophyll fluorescence indicated the presence of stress in the coastal sand dune environment. The net photosynthesis rate (PN) and Y(II) were highest in June in the coastal sand dune environment and July in the pot environment. In August and September, Y(NPQ) increased in the plants in the coastal sand dune environment, showing their photoprotective mechanism. Chlorophyll a and b contents in the pot plant leaves were higher than those in the coastal sand dune plant leaves; however, chlorophyll-a/b ratio was higher in the coastal sand dune plant leaves than in the pot plant leaves, suggesting a relatively high photosynthetic efficiency. Carotenoid content in the coastal sand dune plant leaves was higher in August and September 2022 than that in the pot plant leaves. Leaf water and soluble carbohydrate contents of the coastal sand dune plant leaves decreased in September 2022, leading to rapid leaf abscission. Diurnal variations in photosynthesis and chlorophyll fluorescence in both environments showed peak activity at 12:00 hour; however, the coastal sand dune plants had lower growth rates and Y(II) than the pot plants. Plants in the coastal sand dunes had higher leaf water and ion contents, indicating that they adapted to water stress through osmotic adjustments. However, plants growing in the coastal sand dunes exhibited reduced photosynthetic activity and accelerated decline due to seasonal temperature decreases. These findings demonstrate the adaptation mechanisms of V. rotundifolia to water stress, poor soils, and high temperature conditions in coastal sand dunes. Conclusions: The observed variations indicate the responses of the V. rotundifolia to environmental stress, and may reveal its survival strategies and adaptation mechanisms to stress. The results provide insights into the ecophysiological characteristics of V. rotundifolia and a basis for the conservation and restoration of damaged coastal sand dunes.
Alzheimer's disease (AD) is a neurodegenerative disease characterized by neuronal cell death and memory impairment. Corticosterone (CORT) is a glucocorticoid hormone produced by the hypothalamic-pituitary-adrenal axis in response to a stressful condition. Excessive stress and high CORT levels are known to cause neurotoxicity and aggravate various diseases, whereas mild stress and low CORT levels exert beneficial actions under pathophysiological conditions. However, the effects of mild stress on AD have not been clearly elucidated yet. In this study, the effects of low (3 and 30 nM) CORT concentration on Aβ25-35-induced neurotoxicity in SH-SY5Y cells and underlying molecular mechanisms have been investigated. Cytotoxicity caused by Aβ25-35 was significantly inhibited by the low concentration of CORT treatment in the cells. Furthermore, CORT pretreatment significantly reduced Aβ25-35-mediated pro-apoptotic signals, such as increased Bim/Bcl-2 ratio and caspase-3 cleavage. Moreover, low concentration of CORT treatment inhibited the Aβ25-35-induced cyclooxygenase-2 and pro-inflammatory cytokine expressions, including tumor necrosis factor-α and interleukin-1β. Aβ25-35 resulted in intracellular accumulation of reactive oxygen species and lipid peroxidation, which were effectively reduced by the low CORT concentration. As a molecular mechanism, low CORT concentration activated the nuclear factor-erythroid 2-related factor 2, a redox-sensitive transcription factor mediating cellular defense and upregulating the expression of antioxidant enzymes, such as NAD(P)H:quinone oxidoreductase, glutamylcysteine synthetase, and manganese superoxide dismutase. These findings suggest that low CORT concentration exerts protective actions against Aβ25-35-induced neurotoxicity and might be used to treat and/or prevent AD.
The study aimed to investigate a novel approach by utilizing liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) to separate, identify and characterize very nominal quantities of degradation products (DPs) of balsalazide along with its process related impurities without isolation from their reaction mixtures. The impurities along with balsalazide were resolved on spherisorb ODS2 (250×4.6 mm, 5.0 ㎛) column at room temperature using 0.2 M sodium acetate solution at pH 4.5 and methanol in the ratio of 55:45 (v/v) as mobile phase pumped isocratically at 1.0 mL/min as mobile phase and UV detection at 255 nm. The method shows sensitive detection limit of 0.003 ㎍/mL, 0.015 ㎍/mL and 0.009 ㎍/mL respectively for impurity 1, 2 and 3 with calibration curve liner in the range of 50-300 ㎍/mL for balsalazide and 0.05-0.30 for its impurities. The balsalazide pure compound was subjected to stress studies and a total of four degradation products (DPs) were formed during the stress study and all the DPs were characterized with the help of their fragmentation pattern and the masses obtained upon LC-MS/MS. The DPs were identified as 3-({4-[(E)-(4-hydroxyphenyl) diazenyl]benzoyl}amino)propanoic acid (DP 1), 4-[(E)-(4-hydroxyphenyl)diazenyl] benzamide (DP 2), 5-[(E)-(4-carbamoylphenyl)diazenyl]-2-hydroxybenzoic acid (DP 3) and 3-({4-[(E)-phenyldiazenyl]benzoyl}amino)propanoic acid (DP 4). Based on findings, it was concluded that, the proposed method was successfully applicable for routine analysis of balsalazide and its process related impurities in pure drug and formulations and also applicable for identification of known and unknown impurities of balsalazide.
Background: Ischemia reperfusion injury is known to contribute to the major causes of the early graft failure in lung transplantation. Triiodothyronine (T3) has been suggested to ameliorate ischemia reperfusion injury from both in vivo and in vitro experiments of various organs. Prospecting its beneficial effect for pulmonary allograft preservation, we made a new solution by adding T3 into the extracellular type dextran solution. Material and Method: Twelve adult mongrel dogs underwent left lung allotransplantation. Six donor dogs were flushed with the new solution(Group 1, n=6), and the remaining six were flushed with Euro-Collins solution to serve as controls(Group 2, n=6). Allografts were stored in each preservation solution for 20 hours at 4$^{\circ}C$. Left single lung transplantations were performed. The right pulmonary artery and the right main bronchus were clamped at 15 minutes after the reperfusion and maintained throughout the experiment to evaluate the transplanted left lung function. Result: Arterial carbon dioxide tension was better in group 1 than in group 2 throughout the experiment period and the difference was statistically significant at 2 hours after reperfusion(28.0${\pm}$3.0 mmHg and 53.1${\pm}$17.4 mmHg, p<0.05). The differences of arterial oxygen partial pressure, peak airway pressure and pulmonary vascular resistance showed no statistical significance. The malondialdehyde(MDA) level, measured from tissue obtained at 120 minutes after reperfusion showed no statistically significant difference. The tissue wet/dry ratio of group 1(649${\pm}$27 %) was significantly lower than that of group 2(686${\pm}$71 %, p<0.05). The microscopic examination revealed varying degrees of injury represented mainly by findings such as perivascular neutrophil infiltration, capillary hemorrhage and interstitial congestion. These findings were less severe in group 1 than those in group 2. Conclusion: The new solution demonstrated superior allograft preservation after 20 hour ischemia compared to Euro-Collins solution in canine single left lung transplantation model, these results suggest that T3 might be a promising agent for pulmonary allograft preservation.
A pot experiment was conducted to find out the effects of application of slacked lime and fused super-phosphate on the lead uptake of upland crops in a lead added soil. Lead concentration of the soils were adjusted to 0, 150, 300mg/kg respectively. The slacked lime was applied at the equivalent amount of lime requirement with extra 150kg/10a, and 2 times for the fused superphosphate. The results obtained were as follows: 1. Lead contents in crops increased in the order: sesame > maize > potato > sweet potato > soybean > green perilla > peanut > red bean. 2. Lead contents in parts of crops were increased in the order; root > stem > leaf > grain. 3. Increasing lead concentration in soils, lead content in the plant was increased and crops yield were decreased. 4. Lead contents in soybean and green perlilla were decreased in slacked lime application treatment. 5. The lead contents in leaf and grain of soybean and green perllila decreased with decreasing in the ratio of Pb/Ca+Mg equivalent in soil. 6. Grain yield were increased in slacked lime, but were decreased in fused superphosphate application treatment. 7. With increasing the soil Pb contents, calcium and phosphate contents were increased in leaf and stem, but calcium was decreased in roots. 8. $1N-NH_4$ OAC soluble Pb contents in soil were 26-50 ppm and 42-70 ppm, respectively, for 150mg/kg and 300mg/kg lead treatments. 9. The soil pH was increased in the order of slacked lime, fused superphosphate and nontreatment.
Jeong, Do Hyeon;Shin, Hyeon Ho;Jung, Seung Won;Lim, Dhong Il
Korean Journal of Environmental Biology
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v.31
no.1
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pp.19-36
/
2013
Physiochemical characters of sea waters during summer flood- and winter dry-seasons and their spatial variations were investigated along the coastal area off the eastern South Sea, Korea. Using the hierarchical clustering method, in this study, we present comprehensive analyses of coastal waters masses and their seasonal variations. The results revealed that the coastal water of the study area was classified into six water masses (A to F). During summer season, the surface water was mainly occupied by the coastal pseudo-estuarine water (water mass B) with low salinity and high nutrients and the river-dominated coastal water (water mass C) with low nutrients, respectively. The bottom water was dominated by cold water (water mass D) with very low temperature, high salinity and high nutrients, compared to masses of surface water. Notably, the water mass B, with high concentrations of nutrients (silicate and nitrogen) and low salinity, which is strongly controlled by the water quality of river freshwater, seems to play an important role in controlling the water quality and further regulating physical processes on ecosystem in the eastern coastal area of South Sea. The water mass D (bottom cold water) coupled with a strong thermocline, which exists in near-bottom layer along the western margin of Korea Strait, has a low temperature, pH and DO, but abundant nutrients. This water mass disappears in winter owing to strong vertical mixing, and subsequently may act as a pool for nutrients during winter dry-season. On the other hand, vertically well-mixed water column during the winter season was typically occupied by the Tsushima (water mass E) and the coastal water (water mass F) with a development of coastal front formed in a transition zone between them. These winter water masses were characterized by low nutrient concentration and balance in N/P ratio, compared with summer season with high nutrient concentrations and strong N-limitation. Accordingly, the analysis of water masses will help one to better chemical and biological processes in coastal area. In most of the study area, characteristically, the growth of phytoplankton community is limited by nitrogen, which is clearly different with coastal environment of West Sea of Korea, with a relative lack of phosphorus. It showed the western and the southern coasts in Korea are substantially different from each other in environmental and ecological characteristics.
This study was carried out to estimate the effect of NPK fertilization on tree seedling growth and chemical properties of soil. Seedlings of Betula platyphylla var. japonica and Fraxinus rhynchophylla were planted in May 1998 in the nursery at Gariwang mountain, Kangwondo, and seven NPK fertilizer plots(4, 12, 28, 60, 124, 252, and $508g/m^2$) and one non-fertilized plot were used to meet the study objectives. Seedlings of Pinus koraiensis and Acer mono were planted in April 1998 in plastic pots of nursery at Chungnam National University, and seven NPK fertilizer plots(0.5, 1.5, 3.5, 7.5, 15.5, 33.5 and 63.5g) and one non-fertilized plot were used to meet the study objectives. To estimate the relative growth ratios of the seedlings, height and root collar diameter of the seedlings were measured after planting and also in October 1998. Soil samples were collected in October 1998 from the nursery of Gariwang mountain and the plastic pot of the nursery of Chungnam National University. The relative growth ratios of height and root collar diameter in Betula platyphylla var. japonica, Fraxinus rhynchophylla and Acer mono were greater in all fertilized plot than non-fertilized plot, but there was no difference in relative growth ratio of height and root collar diameter of Pinus koraiensis between the fertilized and non-fertilized plots. NPK fertilization increased soil P and K, decreased exchangeable Ca and Mg, and base saturation, and pH. Fertilization resulted in no difference in total soil N between the fertilized and non-fertilized plots of nursery of Gariwang mountain, but increased in total soil N in plastic pots. Fertilization increased the growth of hardwood species and had changed soil chemical properties of this fertilizer trials.
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