• Journal of Chest Surgery
• /
• 제54권1호
• /
• pp.9-16
• /
• 2021

Background: The deposition of monomeric C-reactive protein (mCRP) in the myocardium aggravates ischemia-reperfusion injury (IRI) and myocardial infarction. Ischemic preconditioning (IPC) is known to protect the myocardium against IRI. Methods: We evaluated the effects of IPC on myocardium upon which mCRP had been deposited due to IRI in a rat model. Myocardial IRI was induced via ligation of the coronary artery. Direct IPC was applied prior to IRI using multiple short direct occlusions of the coronary artery. CRP was infused intravenously after IRI. The study included sham (n=3), IRI-only (n=5), IRI+CRP (n=9), and IPC+IRI+CRP (n=6) groups. The infarcted area and the area at risk were assessed using Evans blue and 2,3,5-triphenyltetrazolium staining. Additionally, mCRP immunostaining and interleukin-6 (IL-6) mRNA reverse transcription-polymerase chain reaction were performed. Results: In the IRI+CRP group, the infarcted area and the area of mCRP deposition were greater, and the level of IL-6 mRNA expression was higher, than in the IRI-only group. However, in the IPC+IRI+CRP group relative to the IRI+CRP group, the relative areas of infarction (20% vs. 34%, respectively; p=0.079) and mCRP myocardial deposition (21% vs. 44%, respectively; p=0.026) were lower and IL-6 mRNA expression was higher (fold change: 407 vs. 326, respectively; p=0.376), although the difference in IL-6 mRNA expression was not statistically significant. Conclusion: IPC was associated with significantly decreased deposition of mCRP and with increased expression of IL-6 in myocardium damaged by IRI. The net cardioprotective effect of decreased mCRP deposition and increased IL-6 levels should be clarified in a further study.

• The Korean Journal of Physiology and Pharmacology
• /
• 제22권1호
• /
• pp.17-21
• /
• 2018

Our previous studies have confirmed that morroniside has neuroprotective effects. However, the effects of morroniside on cardiac myocardium remain unknown. Rats were anaesthetized with 10% chloral hydrate (0.35~0.4 mL/kg) and an acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD). Following AMI, morroniside was administered intragastrically for 3 consecutive days at doses of 45, 90 and 180 mg/kg, respectively. Lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) activities in AMI rats in the serum were detected with commercial kits. The expression of IL-6, $IL-1{\beta}$ and $TNF-{\alpha}$ in myocardium was detected by Western blotting analysis. We observed a significant decline in the Q(q) wave amplitude in morroniside-treated rats after 72 h. Additionally, treatment of morroniside decreased the levels of LDH and cTnT in AMI rats. We also observed that morroniside reduced the expression of IL-6, $IL-1{\beta}$ and $TNF-{\alpha}$ in myocardium. Taken together, our findings demonstrate that morroniside had effective anti-inflammatory properties in AMI rats.

• 대한자기공명의과학회:학술대회논문집
• /
• 대한자기공명의과학회 2003년도 제8차 학술대회 초록집
• /
• pp.43-43
• /
• 2003

Viable myocardium can be distinguished from the infarcted myocardium by contrast-enhanced magnetic resonance imaging (ceMRI). In this study, contrast-enhancement with cine magnetic resonance imaging (cecineMRI) was performed for direct correlation of transmural extent of hyperenhancement and that of contractility.

• 대한자기공명의과학회:학술대회논문집
• /
• 대한자기공명의과학회 2003년도 제8차 학술대회 초록집
• /
• pp.89-90
• /
• 2003

Viable myocardium can be distinguished from the infarcted myocardium by contrast-enhanced magnetic resonance imaging (ceMRI). In this study, contrast-enhancement with cine magnetic resonance imaging (cecineMRI) was performed for direct correlation of transmural extent of hyperenhancement and that of contractility.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.95-97
• /
• 2003

본 연구에서는 손상된 심근의 재생을 위하여 골수단핵세포를 SD 래트에 이식하였고 5주 후에 심근의 재생, 신생혈관의 형성과 더불어 심장의 기능이 향상되었음을 확인할 수 있었다. 골수단핵세포를 손상된 심근경색 부위에 넣어주는 것은 추가 보완 실험을 통하여 심근경색의 치료법으로서 이용될 수 있을 것이다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XIII)
• /
• pp.164-166
• /
• 2003

본 연구에서는 손상된 심근의 재생을 위하여 골수단핵세포를 피브린 고분자와 함께 SD 래트에 이식하였고 8주 후에 신생혈관의 형성과 더불어 심장의 기능이 향상되었음을 확인할 수 있었다. 이 연구는 세포이식을 통한 조직 재생시 세포 이식용 매트릭스의 중요성을 보여준다. 환자 자신의 골수세포를 사용한다면 면역 문제가 없어서, 이 방법이 심부전 환자의 치료에 쉽게 이용될 수 있을 것이다.

• Korean Journal of Radiology
• /
• 제2권1호
• /
• pp.21-27
• /
• 2001

Objective: To identify and evaluate the lateral border zone by comparing the size and distribution of the abnormal signal area demonstrated by MR imaging with the infarct area revealed by pathological examination in a reperfused myocardial infarction cat model. Materials and Methods: In eight cats, the left anterior descending coronary artery was occluded for 90 minutes, and this was followed by 90 minutes of reperfusion. ECG-triggered breath-hold turbo spin-echo T2-weighted MR images were initially obtained along the short axis of the heart before the administration of contrast media. After the injection of Gadomer-17 and Gadophrin-2, contrast-enhanced T1-weighted MR images were obtained for three hours. The size of the abnormal signal area seen on each image was compared with that of the infarct area after TTC staining. To assess ultrastructural changes in the myocardium at the infarct area, lateral border zone and normal myocardium, electron microscopic examination was performed. Results: The high signal area seen on T2-weighted images and the enhanced area seen on Gadomer-17-enhanced T1WI were larger than the enhanced area on Gadophrin-2-enhanced T1WI and the infarct area revealed by TTC staining; the difference was expressed as a percentage of the size of the total left ventricle mass (T2= 39.2 %; Gadomer-17 =37.25 % vs Gadophrin-2 = 29.6 %; TTC staining = 28.2 %; p < 0.05). The ultrastructural changes seen at the lateral border zone were compatible with reversible myocardial damage. Conclusion: In a reperfused myocardial infarction cat model, the presence and size of the lateral border zone can be determined by means of Gadomer-17- and Gadophrin-2-enhanced MR imaging.

• International Journal of Stem Cells
• /
• 제17권1호
• /
• pp.70-79
• /
• 2024

The efficacy of adipose-derived stem cells (ASCs) on myocardial infarction is limited due to poor survival and engraftment. Integrin-mediated cell adhesion is a prerequisite for its survival and homing. ASCs expressed insufficient integrin 𝛼₄, limiting their homing capacity. This study aims to characterize integrin 𝛼₄⁺ ASC subpopulation and investigate their therapeutic efficacy in myocardial infarction. We used fluorescence-activated cell sorting to harvest integrin 𝛼₄⁺ ASCs subpopulation, which were characterized in vitro and transplanted into myocardial infarction model. Positron emission tomography imaging were performed to measure infarction size. Cardiac cine magnetic resonance imaging was used to evaluate heart contractile function. Compared with the unfractionated ASCs, integrin 𝛼₄⁺ ASCs subpopulation secreted a higher level of angiogenic growth factors, migrated more rapidly, and exhibited a stronger anti-apoptotic capacity. Vascular cell adhesion molecule-1 was obviously up-regulated at 3 days after myocardial infarction, which interacted with integrin α₄ receptor on the surface of ASCs to enhance the survival and adhesion. Thus, we implanted unfractionated ASCs or integrin α₄⁺ ASCs subpopulation into the 3-day infarcted myocardium. Integrin α₄⁺ ASCs subpopulation exhibited more robust engraftment into the infarcted myocardium. Integrin α₄⁺ ASCs subpopulation more effectively decreased infarct size and strengthen cardiac function recovery than did the unfractionated ASCs. Integrin α₄⁺ ASCs subpopulation is superior to unfractionated ASCs in ameliorating ischemic myocardial damage in animal model. Mechanistically, their more robust engraftment into the infarct area, higher migratory capacity and their increased release of paracrine factors contribute to enhanced tissue repair.

• Nuclear Medicine and Molecular Imaging
• /
• 제42권6호
• /
• pp.478-481
• /
• 2008

Reverse redistribution is frequently observed after revascularization in acute myocardial infarction, and usually regarded as a predictor of viable myocardium on stress/rest and 2- to 4-hour redistribution $^{201}Tl$ SPECT. However, there is not enough report of reverse redistribution in case of 24-hour delayed SPECT, which is commonly used for viability assessment. In this report, a case of reverse redistribution on rest and 24-hour delayed $^{201}Tl$ SPECT is reported with use of automatic segmental quantitative analysis. The myocardium of reverse redistribution was dysfunctional on gated SPECT, and diagnosed as non-viable on $^{18}F-FDG$ PET.

• Investigative Magnetic Resonance Imaging
• /
• 제7권2호
• /
• pp.132-136
• /
• 2003

목적 : 심근경색을 가진 환자에서 호흡정지 T2강조 자기공명영상의 유용성을 알아보고자 하였다. 대상 및 방법 : 심근 생존능을 평가하기 위하여 자기공명영상이 시행된 환자에서 지연 조영증강을 보였던 11명의 환자를 대상으로 하였다. 지연 조영증강을 보이는 심근부위와 비교하여 T2강 조영상에서 일치하는 부위를 고신호강도, 동등신호강도, 저신호강도로 나누어 분석하였으며, 정상 심근과 경색 부위의 신호강도 세기 및 통벽성 범위(transmural extent)을 측정하였다. 환자의 임상정보를 토대로 심근경색의 시기와 T2 강조영상과의 상관관계를 분석하였다. 결과 : T2강조영상에서 5명의 환자의 12분절에서 정상심근에 비하여 경색부위에 고신호강도를 보였으며, 6명의 환자의 12분절에서 저신호강도를 보였다. 경색부위의 고신호강도는 정상심근에 비하여 $175{\pm}9\%$ 이었으며, 저신호강도는 $73{\pm}5\%$ 이었다 (p < 0.05). 통벽성 범위(transmural extent)의 평가에 있어, T2강조영상에서 고신호강도 부위는 지연 조영증강을 보인 부위보다 컸으나 $(100\%\;vs.\;49{\pm}17\%)$, 저신호강도 부위는 일치하였다. T2 강조영상에서 고신호강도는 심근경색후 11일 이내에 보였고, 저신호강도는 7개월 이후에 보였다. 결론 : 호흡정지 T2 강조영상은 부종 및 섬유화 반흔 범위의 평가와 함께 심근경색의 시기를 예측하는 데에 있어 유용할 것으로 사료된다.