• Journal of Korean Society of Forest Science
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• v.79 no.3
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• pp.302-308
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• 1990

This study was conducted to determine the effects of mycorrhizal inoculation and ortet ages on the rooting of Quercus acutissima cuttings. The cuttings taken from 12-week-, 2-year-, 4-year-, and 20-year-old plants were rooted in mid summer in the rooting medium (vermiculite 2 : peatmoss 1 by volume) with or without Pisolithus tinctorius (Pt) and Glomus sp. inocula and IBA under intermittent misting system in an open shed. The average percentages of rooting were about 82%, 49%, 29%, and 13% for cuttings taken from 12-week-, 2-year-. 4-year-, and 20-year-old seedlings, respectively. Pt inoculation, enhanced rooting of cuttings at all age classes, except 12-week-old seedlings, with the highest enhancement(22%) observed in cuttings taken from 20-year-old trees. The highest percentage of rooting in each age group eras 88.9% in 12-week-old seedlings treated with Pt plus 3.000ppm IBA, 75% in 2-Year-old plants with 1,000ppm IBA, 58.3% in 4-year-old plants with 3.000ppm IBA and 22% in 20-year-old plants. The addition of Glomus sp. fungus inoculum failed to enhance rooting. Pt mycorrhizal inoculation enhanced root dry weight, length, and diamter of adventitious roots at cuttings taken from 12-week- and 20-year-old trees, except the cuttings taken from 4-year-old seedlings. Rooted cuttings had more total nitrogen content in the leaves than unrooted cuttings, and the greater rooting response was associated with the higher phosphorus content in the leaves.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.68-75
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• 1999

This study was conducted to examine the effects of Arbuscular Mycorrhizae inoculation and phosphorus application on early growth of hot pepper. Gigaspora margarita and Acaulospora spinosa were chosen for this investigation and inoculated into soils of different P levels by varying inoculation time and density. After treatment, some relevant growth responses of hot pepper were measured. Regardless of soil P levels, hot peppers treated with arbuscular mycorrhizal fungi had 5~34% more fresh weight than those untreated, but the effect of inoculation time and density was not different between two species. With decreased P levels, the infection rate and dependency of hot peppers increased. The content of P and K of AMF-inoculated hot peppers increased with increasing P levels, but the shoot to root ratio of those elements decreased. The results of this study showed that inoculation of AMF would be effective in promoting growth of hot pepper seedlings and increase transplant adaptation due in part to the resulted higher root development.

• The Korean Journal of Mycology
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• v.36 no.2
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• pp.148-152
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• 2008

We inoculated hypal suspension of Tricholoma matsutake and T. magnivelare were examined on Pinus densiflora seedlings grown in a granite soil substrate with 1/2 PDMP (12 g/l potato dextrose broth, 1.5 g/l malt extract, and 0.5 g/l peptone) medium. Four months after inoculation, the pine seedlings were examined for infection rate, matsutake aroma, and Hartig-net formation. The roots of pine seedling formed ectomycorrhizal roots in the 9 isolates from 12 isolates of T. matsutake and T. magnivelare. However, the seedlings showed different ectomycorrhizae forming rates among the 9 isolates. While matsutake aroma was confirmed from the ectomycorrhizal seedlings, the pine seedling contaminated by bacteria or fungi did not form matsutake ectomycorrhizae with sickening smell. Thus, the aroma was chosen as a good way for the verification of mycorrhizal infection. At the early stage, the mycorrhizal roots showed unramified and branched types without root hair. They also showed thin mantle layers, Hartig-nets, and turned into black color at later stage. Among the examined strains, that of Yecheon isolated in 1995 showed the best infection rate, which indicated that we need to pay attention to the selection of isolates for better result.

• Journal of Korean Society of Forest Science
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• v.85 no.1
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• pp.96-106
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• 1996

Red alder(Alnus rubra Bong.) seedlings inoculated with Frankia only or both Frankia and spores of Alpova diplophloeus(Zeller & Dodge) Trappe & Smith were grown in a greenhouse for ten weeks. The ten-week-old seedlings were fertilized with six nitrogen(N) and phosphorus(P) fertility regimes (no fertilization, 10mM $NH_4NO_3$, 50mM $NH_4NO_3$, 5mM $KH_2PO_4$, 10mM $NH_4NO_3+5mM$ $KH_2PO_4$, and 50mM $NH_4NO_3+5mM$ $KH_2PO_4$) three times a week for ten weeks. The higher N-fertilization significantly increased mycorrhiza formation by greenhouse contaminant mycorrhizal fungi, but decreased N-fixation and P concentration in nodule tissues. P-fertilization significantly increased nodule and shoot dry weight, and P concentration in plant tissues. When N was highly fertilized, however, the P-fertilization effect disappeared in nodule P concentration but doubled in leaf P concentration. A. diplophloeus inoculation significantly increased diameter growth and $CO_2$ exchange rate, but decreased leaf dry weight. Our results suggest that the higher N- or P-fertilization affect nitrogenase activity and mycorrhizal development but the effects are changed by their interactions.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.2
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• pp.100-106
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• 2014

The present study investigated variations in soil microbial communities by fatty acid methyl ester (FAME) and the chemical properties at 24 sites of upland soils in Gyeongnam Province. The electrical conductivity of the soil under potato cultivation was significantly higher than those of the red pepper and soybean soils (p < 0.05). The gram-negative bacteria community in potato soil was significantly lower than those in the garlic and soybean soils (p < 0.05). The communities of actinomycetes and arbuscular mycorrhizal fungi in the red pepper soil were significantly higher than those in the potato soil (p < 0.05). In addition, the cy17:0 to 16:$1{\omega}7c$ ratio was significantly lower in red pepper, soybean, and garlic soils compared with potato soil, indicating that microbial stress decreased. Consequently, differences in soil microbial community were highly associated with cultivated crop species, and this might be resulted from the difference in soil chemical properties.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.221-227
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• 2011

Applications of green manures generally improve the soil quality in rice paddy in part through restructuring of soil microbial communities. To determine how different green manures affect soil microbial communities during the early stages of rice growth, fatty acid methyl ester (FAME) profiles were used to the effects of different management practices: 1) conventional farming (CF), 2) no-treatment (NT), 3) Chinese milk vetch (CMV), 4) green barley (GB), and 5) triticale in paddy field. With applications of green manures, soil organic matter was significantly higher than CF, while soil Na concentration was significantly lower compared with CF (p<0.05). Total soil microbial biomass of CMV was higher (p<0.05) than NF by approximately 31%. The highest ratio of monounsaturated fatty acid to saturated fatty acid was found in the GB plot, followed by CMV and triticale compared with CF (p<0.05), possibly indicating that microbial stress was less in GB and CMV plots. Populations of Gram-negative bacteria and arbuscular mycorrhizal fungi also were significantly higher green manures than CF (p<0.05). Our findings suggest that GB should be considered as optimum green manure for enhancing soil microbial community at an early growing stage in paddy field.

• Journal of Forest and Environmental Science
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• v.34 no.6
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• pp.481-489
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• 2018

In this study, the succession and stand dimension attributes related to the disaster prevention function of Pinus thunbergii coastal forests were examined after damage from Diplodia tip blight. In 2015, 101 years after the Taisho eruption, field investigations were performed on the vegetation, soil thickness, and pH of surface soil of P. thunbergii coastal forests in western Sakurajima (Hakamagoshi plot) and Taisho lava flows in southeastern Sakurajima (Seto plot). The Hakamagoshi plot had more woody plant species with larger basal areas than that in the Seto plot. The mean age and height, maximal age and height of plant species, and H/D ratio were all larger in the Hakamagoshi plot than in the Seto plot. These results may be explained by the relatively smaller effect of volcanic ash and gas on forests in the Hakamagoshi plot compared to the Seto plot, resulting in a more suitable environment for many plant species. Although P. thunbergii coastal forests in Sakurajima are currently recovering from damages owing to Diplodia tip blight, there has not yet been a sufficient recovery compared to the results from a 1997 study. Furthermore, the results of assessment based on the H/D ratio and abundance of trees in P. thunbergii forests indicate that both regions are not yet effective in disaster prevention. Thus, it is necessary to establish Pinus trees, which can adjust to harsh environments like coastal areas and are resistant to volcanic ash and gas, to enhance the disaster prevention function of P. thunbergii coastal forests in volcanic regions. It may also be helpful to establish coastal forests with ectotrophic mycorrhizal fungi and organic matter coverage. Additionally, it is necessary to ensure the continuous maintenance of stand density and soil quality, and further develop efforts to prevent Diplodia tip blight and promote forest recovery.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.164-170
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• 2011

Amanita hemibapha is a kind of mycorrhizal mushroom which has a relation with host plants and environmental factors. For the purpose of studying the relationship between environmental factors and mushrooms, in this study we conducted to investigate the soil physicochemical properties, the distribution of plants and the diversity of mushrooms in Gwangyang, Chungdo and Daegu. Soil texture in habitate of Amanita hemibapha was Sandy Loam and Sandy Clay Loam which contains a high rate of sand. Soil pH was approximately 5.0 in all study sites. Total nitrogen contents, C/N ratio and available phosphate($P_2O_5$) were the highest at the Chungdo. However, Gwangyang and Chungdo represent similar Cation Exchange Capacity(C.E.C) and Daegu has much less than other areas. Exchangeable cations, $Ca^{2+}$ content was higher at Gwangyang and Chungdo than Daegu and $Na^+$ content was the highest at Chungdo among three sites. Daegu has much $Mg^{2+}$ contents followed by Chungdo and Gwangyang. Interestingly, $K^+$ content listed in reverse order of $Mg^{2+}$ at Gwangyang, Chungdo and Daegu. The main woody plants in study sites are confirmed as Carpinus laxiflora, Quercus mongolica, Q. serrata and Pinus densiflora. Quercus species are found as a common species in three study sites. Lastly, 8 family and 12 species of mushrooms are emerged in Gwangyang, also 8 family and 12 species and 5 family and 10 species are found in Chungdo and Daegu, respectively. Amanita pantherina, Boletus edulis, Tylopilus felleus and Marasmius maximus, which found in study sites, are also kind of mycorrhizal mushroom same as A. hemibaph. By using correspondence analysis, Q. mongolica, Q. serrata, Q. variabilis and C. laxiflora are expected to as a host plant of A. hemibapha.

• Horticultural Science & Technology
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• v.17 no.5
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• pp.578-580
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• 1999

The effects of the orchid mycorrhizal fungi isolated from Cymbidium goeringii were investigated on the growth of orchid plants. The plants, a hybrid of Cymbidium kanran Jeju${\times}$C. kanran Nangoku and Phalaenopsis were inoculated with the fungal isolates of Rhizoctonia repens (P1), R. endophytica (P2) and R. repens (P3; different from P1) on the oatmeal agars for two months. Then the orchid plants were cultivated in the greenhouse for eighteen months. The difference of plant growth after cocultivation for two months was not found among the treatments. After cultivation for four months, the growth of hybrid plants of Cymbidium kanran Jeju${\times}$C. kanran Nangoku was observed to be distinguished in the Rhizoctonia repens (P1). After cultivation in the greenhouse for eighteen months, the plant height, the number of shoots, the number of leaves, the number of roots and plant fresh weight of the hybrid of Cymbidium kanran Jeju${\times}$C. kanran Nangoku, inoculated with Rhizoctonia repens (P1), were significantly increased compared to those of the control. The plants inoculated with Rhizoctonia repens (P3) were not different and the plants inoculated with R. endophytica were poor compared to the control. The plant height and fresh weight of the Phalaenopsis, inoculated with Rhizoctonia repens (P1), increased significantly compared to those of the control. Overall, Rhizoctonia repens (P1), was the most effective for the growth of a hybrid of Cymbidium kanran Jeju${\times}$C. kanran Nangoku.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.261-269
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• 2012

Soil microbial community and soil physiological parameters were investigated by analyzing phospholipid fatty acids extracted from the soils amended with chitosan powder and solution in a cucumber greenhouse. The soils were sampled at 90, 160, 200 days after treatment. Identified fatty acids were analyzed with principal component (PC) analysis. Chitosan powder soils and chitosan solution soils were separated from non-treated control soils by PC1 and PC2 90 days after treatment, respectively. And chitosan powder soils were separated from non-treated control soils by PC2 160 days after treatment. The ratio of fungi to bacteria increased significantly in chitosan solution-amended soils compared with the control soils 90 days after treatment. Microbial groups and physiological parameters were investigated 160 days after treatment: vesicular-arbuscular mycorrhizal fungi (VAM) significantly increased in soils amended with chitosan powder compared with other soils, the ratio of gram negative bacteria to gram positive bacteria and cyclo-fatty acids to precursors were significantly higher and lower in soils amended with chitosan solution and chitosan powder compared with control soils, respectively, and the ratio of fungi to bacteria were significantly lower in control soils compared with chitosan-treated soils. The chitosan powder increased the ratio of aerobic to anaerobic bacteria and lowered the ratio of saturated to unsaturated fatty acids compared with chitosan solution 200 days after soil application. In conclusion, chitosan powder changed the soil microbial community and the effects maintained up to 160 days after soil application. The effect of physiological parameters on the soil microbial community started to appear 160 days after and continued up to 200 days after soil application of chitosan.