• Mycobiology
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• v.37 no.1
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• pp.72-76
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• 2009

To investigate the growth response of various crop species to mycorrhizal inoculation, arbuscular mycorrhizal fungi were applied to Glycine max, Vigna angularis, Senna tora, Hordeum vulgare var. hexastichon. Zea mays, Sorghum bicolor, Allium tuberosum, Solanum melongena, and Capsicum annuum. The biomass of the inoculated crops was measured every two weeks for the 12-week growth period. By measuring biomass, we calculated the mycorrhizal responsiveness of the nine crop species. Among the nine crop species, four species showed a significant response to mycorrhizal inoculation. The shoot biomasses of V. angularis, C. annuum, A. tuberosum, and S. tora significantly increased with mycorrhizal inoculation.

• Mycobiology
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• v.32 no.2
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• pp.79-87
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• 2004

An experiment was carried out to investigate the effects of pre-inoculation with the mycorrhizal fungus Glomus clarum and foliar application of kinetin on the growth of mungbean plant irrigated wht different dilution of seawater. Arbuscular-mycorrhizal(AM) infection significantly increased dry weight, height, chlorophyll, sugar and protein content, nitrogen and phosphorus-use efficiencies, leaf conductivity, transpiration rate, nitrogenase, acid and alkaline phosphates activities of all salinized mungbean plants in comparison with control and non-mycorrhizal plants irrespective of the presence or absence of kinetin. Mycorrrhizal plants showed higher concentrations of N, P, K, Ca and Mg and lower Na/N, Na/P, Na/K, Na/Ca and Na/Mg ratios than non-mycorrhizal plants when irrigated with certain dilution of seawater. Mungbean plants showed 597% and 770% dependency on AM fungus G. clarum in absence and presence of kinetin, respectively, for biomass production under a level of 30% of seawater. The average value of tolerance index for mycorrhizal plants accounted 267% and 364% in absence and presence kinetin respectively. This study provides evidence for the benefits of kinetin which are actually known for mycorrhizal than non-mycorrhzal plants. AM fungus and kinetin protected the host plants against the detrimental effects of salt. However, mycorrhizal infection was much more effective than kinetin applications. Thus management applications of this arbuscular mycorrhizal endophyte(G. clarum) with kinetin could be of importance in using seawater in certain dilution for irrigation in agriculture.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.1
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• pp.54-63
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• 1994

Runner-derived(Expt.1) and tissue culture-derived strawbeery plantlets(Expt. 2) were grown in pots under greenhouse condition and inoculated with inocula of the vesicular-arbuscular mycorrhizal(VAM) fungi isolated from a field strawberry plants. Total biomass of mycorrhizal strawberry plants was significantly increased. There was a similar tendency in the number of cluster and flower at 20 weeks after inoculation, and VAM fungi inoculation positively influenced the leaf number, leaf length, leaf width and petiole length of strawberry plants in all investigated times. However, no difference was in the flowering time of strawberry plants. Leaf margin of non-inoculated strawberry plantlets turned into raddish brown(7.5R 4/8) from around 4 weeks after habituation. Inoculation of VAM fungi at the time of habituation was much more effective in stimulating plant growth. VA mycorrhizal dependency were 162.7 % in the runner-derived strawberry plants, Dependency with pre-and post-habituated incoulation in tissue culture-derived plants was respective 116.4% and 106.0%. The levels of mycorrhizal colonization were increased with plant growth and infection rates by endophytes at harvest time were 47.5% in Expt. 1, 56.4% in Expt. 2, respectively. Contents of phosphorus, potassium and calcium in mycorrhizal strawberry plants at harvest time were higher than non-mycorrhizal ones however, magnesium concentration was decreased. These experiments demonstrated that VAM fungi could be introduced into nursery stages of strawberry plantlets including the temporary planting period to improve growth and plant nutrients uptake by mycorrhizal plants.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.6
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• pp.482-492
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• 2000

Vesicular arbuscular mycorrhizal (VAM) fungi are known to increase plant growth as well as to enhance salt tolerance of plants where plant roots are colonized by VAM. In pot experiment, pepper was grown in soil containing 0, 200, 400, and $600P\;kg\;ha^{-1}$ with and without mycorrhizal inoculum. Pots were irrigated with saline water containing 0.5, 2.0, and $6.0dS\;m^{-1}$. At 0, 200, and $400P\;kg\;ha^{-1}$ of three EC treatments, plant hight in mycorrhizal treatments was significantly different compared to nonmycorrhizal treatments. However, plant hight at $600P\;kg\;ha^{-1}$ was not different between mycorrhizal and nomycorrhizal treatments. Leaf area at $0P\;kg\;ha^{-1}$ of three EC treatments, and $200P\;kg\;ha^{-1}$ of $6.0dS\;m^{-1}$ in mycorrhizal treatments significantly increased compared to nonmycorrhizal treatments. However, these increase were not discovered in high salinity and P level. Level of EC affected dry weight, and especially, interection of P and EC, or P and VA inoculation highly affected root dry weight. R/S ratio generally decreased in mycorrhizal treatments. Significantly decreased R/S ratio was shown at 0, 400, and $600P\;kg\;ha^{-1}$ of $6.0dS\;m^{-1}$. Chlorophyll content generally increased with decreased salinity and P level where mycorrhizal treatments showed higher chlorophyll content compared to nonmycorrhizal treatments. The benefits of VAM inoculation on fruit production was discovered at only low P level and salinity. Mycorrhizal dependency on dry weight basis was generally shown in $0P\;kg\;ha^{-1}$ of three EC treatments and 0.5, $2.0dS\;m^{-1}$ of $200P\;kg\;ha^{-1}$ level. Colonization rate ranged 3.3 to 43.3% and number of spores was 47.7 to 198.3 $100g^{-1}$ soil. Colonization rate and number of spores increased with decreased P level and salinity where there was high correlation ($r=0.858^{**}$) between both. Also improved uptake of mineral nutrients was discovered at mycorrhizal treatments in decreased P level and salinity.

• Proceedings of the Ginseng society Conference
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• 1998.06a
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• pp.90-95
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• 1998

Symbiotic associations between land plants and fungi have been known for more than one hundred years. Vesiculararbuscular mycorrhizas (VAM) are the most common symbiosis in flowering plants and can be recognized in almost all plant families. These fungal associations play a very important role in the growth and survival of plant species. However, with respect to the importance and intensity of the VAM, there is great variation among host species. Our knowledge of the VAM fungus-plant association in Araliaceae is very limited. After the first reports of the occurence of VAM in lateral roots of Panax species, mycorrhizal structures are now described as special structures representing the so-called Paris type. In this type, the development of new spores and vesicles is extremely low. This and the type of colonization of the fungus in Panax roots indicates on, one hand, the high intensity of the VAM and, on the other hand, a remarkable dependency for VAM in members of the Panax species. Therefore, it can be easily understood that cultivated Panax plants exhibit a significant uptake of nutrients and this leads to an extremely depleted soil at harvest. Further, the soil is nearly free of the spores of VAM fungi as they germinate each year on the newly developing Panax roots.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.466-475
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• 1997

I examined arbuscular mycorrhizal(AM) fungus inoculation effects on the seedling growth of Korean ash tree(Fraxinus rhynchophylla Hance), which distributes in fertile mesic soils, under a seven-day watering cycle of water stress and compost-added fertile conditions. Three Korea-native AM fungi were inoculated : an unidentified Glomus species, Gigaspora margarita Becker & Hall and Scutellospora heterogama(Nicol. & Gerd) Walker & Sanders from disturbed forest soils. The effect of AM fungus inoculation on the seedling varied depending upon fungal species and soil conditions. AM formation was 27 to 65% by the Glomus without forming spores, 47 to 74% with about 10 spores per 20g soil by G. margarita and about 65% with 35 spores by S. heterogama. The soil conditions did not affect either AM or spore formation. The Glomus inoculation increased shoot N and P concentrations, but did not affect seedling growth. G. margarita increased shoot N and P, irrespective of soil conditions, in general, but S. heterogama increased N under water stress and Pin the control soil only. These two fungi significantly increased seedling growth in both control and water stress soils. Compost addition increased the growth of non-mycorrhizal seedlings and offset AM fungus inoculation effects. The relative field mycorrhizal dependency(RFMD) of the seedlings was significant only in control and water stress soils by over 40% in G. margarita or S. heterogama AM plants. Under water stress RFMD was the most evident in S. heterogama AM plants. I conclude that some AM fungi such as G, margarita and S. heterogama can broaden the niche of Korean ash seedlings to a water stress or nutrient poor site but less likely to more fertile sites.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.68-75
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• 1999

This study was conducted to examine the effects of Arbuscular Mycorrhizae inoculation and phosphorus application on early growth of hot pepper. Gigaspora margarita and Acaulospora spinosa were chosen for this investigation and inoculated into soils of different P levels by varying inoculation time and density. After treatment, some relevant growth responses of hot pepper were measured. Regardless of soil P levels, hot peppers treated with arbuscular mycorrhizal fungi had 5~34% more fresh weight than those untreated, but the effect of inoculation time and density was not different between two species. With decreased P levels, the infection rate and dependency of hot peppers increased. The content of P and K of AMF-inoculated hot peppers increased with increasing P levels, but the shoot to root ratio of those elements decreased. The results of this study showed that inoculation of AMF would be effective in promoting growth of hot pepper seedlings and increase transplant adaptation due in part to the resulted higher root development.