• Title/Summary/Keyword: Mycerial

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Studies on Rhizina Root Rot Disease of Pinus densiflora : Physiological Characteristics and Pathogenicity of Rhizina undulata (소나무 리지나뿌리썩음병(病)에 관(關)한 연구(硏究) : Rhizina undulata의 생리적(生理的) 특성(特性) 및 병원성(病原性))

  • Lee, Sang Yong;Kim, Wan Kyu
    • Journal of Korean Society of Forest Science
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    • v.79 no.3
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    • pp.322-329
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    • 1990
  • A group of Pinus densiflora trees attacked by Rhizina root rot were observed at Kangnung. Diseased roots are characterized by rot patches, radial rot traces or formation of adhesive soil masses. The damage has proceeded about 6m per annum, and the pathogen in the infected soil was detected by trap logs. Ascospores of Rhizina undulata was germinated by heat shock at $37^{\circ}C$ for 24 hours or at $40^{\circ}C$ for 17 hours. The mycerial growth was optimum on PDA medium at $25-30^{\circ}C$and pH 5.6-6.3. Coniferous trees were more susceptible than non-coniferous trees in inoculation test in vitro.

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Identification and Characterization of Eurotium rubrum Isolated from Mejuin Korea

  • Yun, Yeo-Hong;Hyun, Min-Woo;Suh, Dong-Yeon;Kim, Yong-Min;Kim, Seong-Hwan
    • Mycobiology
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    • v.37 no.4
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    • pp.251-257
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    • 2009
  • We isolated and identified a strain of Eurotium rubrum from Meju that has not been reported in Korea. This fungus is yellowish brown; reverse dark brown on CYA and PDA while yellow on 2% MEA at $25{^{\circ}C}$. Cleistothecia are first bright yellow and gradually turned brown. Mycerial growth on CYA attained a diameter of 30 mm at $20{^{\circ}C}$, 37 mm at $25{^{\circ}C}$ and 32 mm at $30{^{\circ}C}$ after 15 days. The isolate grew slower on 2% MEA ($<$ 20 mm 15 days at $25{^{\circ}C}$) compared to CYA and PDA ($<$ 40 mm 15 days at $25{^{\circ}C}$). Cleistothecia are superficial, yellow to light brown, globose to subglobose, 40~75 ${\mu}m$ in diameter. Asci are 8-spored and globose to subglobose 8~11 ${\mu}m$. Ascospores are disciform, 4.0~5.0 ${\mu}m$ in length and 4.2~4.5 ${\mu}m$ in width. Conidia are ovate or bacillar, finely roughened to densely spinulose, 4.6~6.0 ${\mu}m$ in length and 3.0~4.3 ${\mu}m$ in width. Compared to known Eurotium rubrum, the Korean isolate showed 99% sequence similarity in ITS rDNA (554 bp) and calmodulin (750 bp) gene and 100% in $\beta$-tubulin (1016 bp) gene. The E. rubrum isolate also had weak $\beta$-glucosidase and protease activities.

Cultural characteristics of oyster mushroom (Pleurotus ostreatus) on addition rate of Cudrania tricuspidata (기능성자원 구지뽕나무(Cudrania tricuspidata) 톱밥의 첨가량에 따른 느타리버섯의 재배적 특성)

  • Lee, Chan-Jung;Jhune, Chang-Sung;Cheong, Jong-Chun;Kong, Won-Sik;Suh, Jang-Sun;Park, Gi-Chun;Park, Chun-Geon;Shin, Yu-Su
    • Journal of Mushroom
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    • v.10 no.3
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    • pp.129-135
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    • 2012
  • This study was carried out to investigated optimum mixing ratio of Korean natural Cudrania tricuspidata for production of functional oyster mushroom. Total nitrogen and carbon source of Cudrania tricuspidata was 0.27% and 40.9%, respectively and C/N ratio was 152. Total nitrogen source and pH of substrates mixed with Cudrania tricuspidata were 2.7~2.8 and 4.9~5.1, respectively. The contents of $P_2O_5$, CaO, MgO and $Na_2O$ were no significant difference. Mycerial growth was faster at Cudrania tricuspidata 10% than that of control, but the other treatment was slower. Yields of fruiting body was the highest at Cudrania tricuspidata 20%, and diameter and thick of pileus were no significant difference to increase of Cudrania tricuspidata addition ratio. The L value of pileus was the highest at the Cudrania tricuspidata 20% during mushroom harvest, but there was no significant difference in the a-value and the b-value. Chemical contents of fruiting body were the highest at the Cudrania tricuspidata 30%.

Growth characteristics and productivity of oyster mushroom according to adding of Rhus verniciflua sawdust (옻나무톱밥 첨가에 따른 느타리버섯의 생육 특성 및 수량성)

  • Lee, Chan-Jung;Yoo, Young-Mi;Han, Ju-Yeon;Cheong, Jong-Chun;Jhune, Chang-Sung;Moon, Ji-Won;Kong, Won-Sik;Suh, Jang-Sun
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.232-235
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    • 2014
  • This study was carried out to investigated optimum mixing ratio of Korean natural Rhus verniciflua for production of functional oyster mushroom. Mycerial growth was some fast at addition of R. verniciflua sawdust, and not significant difference by increase of R. verniciflua substrate. Yields of fruiting body show the highest to 150.2 g/850 ml of medium which are addition 20% of R. verniciflua sawdust, and diameter of pileus and thick of stipes were the highest at addition 10% of R. verniciflua sawdust. The L value of stipes were the highest at addition 10% of R. verniciflua sawdust and the L value of pileus were decreased by increase of R. verniciflua substrate, but there was no significant difference in the a-value and the b-value.

Optimal additive ratio of barley flour for substitution of rice bran at cultivation of winter mushrooms (팽이버섯 재배에서 미강 대체용 보리가루의 적정 첨가 비율)

  • Lee, Chan-Jung;Moon, Ji-Won;Yoo, Young-Mi;Han, Ju-Yeon;Cheong, Jong-Chun;Kong, Won-Sik;Lee, Byung-eui
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.266-269
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    • 2015
  • This study was carried out to investigated optimum additive ratio of barley flour for substitution of rice bran at cultivation of winter mushrooms. Mycerial growth was faster according to increase of barley flour ratio than those of controls, but only some slow at the addition of 10% barley flour. Yields of fruiting body show the highest to 165.4 g/850 ml of medium which are the addition 30% of barley flour and not significant difference of yields up to the addition of 70% barley flour. Diameter of pileus was the highest at the addition of 30% barley flour. Hardness of pileus and stipes were the highest at the addition of 10% barley flour. The L value of stipes were the hight at the addition of barley flour, but the L value of pileus were decreased at the addition of barley flour, but there was no significant difference in the a-value and the b-value.

Growth characteristics and productivity of oyster mushroom according to adding of Salvia miltiorrhiza (단삼 첨가에 따른 느타리버섯의 생육 특성 및 수량성)

  • Lee, Chan-Jung;Moon, Ji-Won;Yoo, Young-Mi;Han, Ju-Yeon;Cheong, Jong-Chun;Kong, Won-Sik;Kim, Young Guk;Yoo, Young-Jin
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.262-265
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    • 2015
  • This study was carried out to investigated optimum mixing ratio of Korean cultivation Salvia miltiorrhiza for production of functional oyster mushroom. Mycerial growth was slow at addition of Salvia miltiorrhiza, and significant difference by increase of Salvia miltiorrhiza. Yields of fruiting body show the highest to 139.5 g/850 ml of medium which are addition 5 g/bottle of Salvia miltiorrhiza but rapid decrease by increase of Salvia miltiorrhiza. Diameter of pileus and thick of stipes were higher at addition Salvia miltiorrhiza than those of the controls. Thick of pileus and length of stipes were the highest at addition 30g, and 20 g and 30 g of Salvia miltiorrhiza. The L value of stipes were the highest at addition 20 g and 50 g of Salvia miltiorrhiza and the L value of pileus were the highest at addition 30 g of Salvia miltiorrhiza, but there was no significant difference in the a-value and the b-value.

Optimum mixture ratio of functional Lindera glauca for culture of oyster mushroom (Pleurotus ostreatus) (느타리버섯 재배를 위한 기능성자원 감태나무(Lindera glauca) 톱밥의 적정 혼합비율)

  • Lee, Chan-Jung;Jhune, Chang-Sung;Cheong, Jong-Chun;Kong, Won-Sik;Park, Gi-Chun;Lee, Jeang-Hun;Shin, Yu-Su
    • Journal of Mushroom
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    • v.10 no.1
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    • pp.9-14
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    • 2012
  • This study was carried out to investigated optimum mixing ratio of Korean natural Lindera glauca for production of functional oyster mushroom. Total nitrogen and carbon source of Lindera glauca was 0.16% and 40.9%, respectively and C/N ratio was 215. Total nitrogen source and pH of substrate mixed with Lindera glauca was 2.8~3.0 and 4.8~5.0, respectively. The contents of $P_2O_5$, CaO, MgO and $Na_2O$ were increased by increasing Lindera glauca, but there was no significant difference in $K_2O$ content. Mycerial growth was faster at Lindera glauca treatments than that of control. Yields of fruiting body was the highest at Lindera glauca 20%, and dimeter and thick of pileus were increased according to increase of Lindera glauca addition ratio. The L value of pileus was the highest at the Lindera glauca 10% during mushroom harvest, but there was no significant difference in the a-value and the b-value.

Effect of Temperature on Pathogen Growth and Damage Analysis of Leaf Spot Disease on Grapevine Caused by Paeudocercospora vitis in Korea (포도나무 갈색무늬병균(Pseudocercospora vitis (Lếv.) Speg.)의 생육에 미치는 온도의 영향과 갈색무늬병 발생 포도나무의 피해해석)

  • Park Jong-Han;Han Kyeong-Suk;Lee Jung-Sup;Seo Sang-Tae;Jang Han-Ik;Kim Heung-Tae
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.10-14
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    • 2006
  • This study was carried out to know damages of the grapevine trees by the disease to the leaf spot from 2000 to 2003. The isolates collected from different varieties and locations were identified as Pseudocercospora vitis ($(L\acute{e}v)$.) Speg. based on the morphological and cultural characteristics. According to the in vitro test, the range of temperatures for the mycerial growth and the conidical germination of the fungus were from $10^{\circ}C\;to\;35^{\circ}C$ and optimum temperature was $25^{\circ}C$. There were remarkably different features between a low infection trees group and high infection trees group in terms of number of leaves per fruit branch, length and diameter of internode and leaf area.

Studies on the optimal conditions for the high yield in the production of Ganoderma lucidum (영지버섯 다수확 생산을 위한 최적조건에 관한 연구)

  • Kim, Hong-Kyu;Kim, Yong-Kyun;Seo, Gwan-Seuk;Oh, Se-Hyeon;Kim, Hong-Gi
    • Journal of Mushroom
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    • v.3 no.4
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    • pp.154-158
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    • 2005
  • The results were as follows; 3 additives(rice bran, wheat bran and soybean bran) to the Quercus dentata sawdust were applied at 10, 20 and 30%. High yield was recorded at 20% of rice bran and wheat bran and 30% of soybean bran. Among various sawdust media, the cultivation with the Quercus dentata sawdust alone showed the most favored growth index, such as 22 days of full mycelial growth, 11 days of pinheading from spawning and 23.5g per $1,200m{\ell}$ bottle. In the size of bottles, the lowest infection ratio was 5 % at $1,200m{\ell}$ bottle. In the growth characteristics with the bottle size, Reishi at $1,600m{\ell}$ bottle showed best result when it is observed for 13~15 days in pinheading from spawing, 36~37 days in fruit bodies growing and 26.7~29.3g yield per bottle. The highest yield per area was 6.4kg at horizontal bed with 218 bottles per $3.3m^2$. The Reishi produced in $1,600m{\ell}$ bottle with horizontal cultivation had less number of fruit bodies and best yield, thus it is determined to be the most economic method.

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Occurrence of Botrytis cinerea Resistant to Dicarboximide Fungicides on Strawberries in Greenhouses (시설재배(施設栽培) 딸기 포장(圃場)에서 Dicarboximide 계(系) 약제저항성(藥劑抵抗性) 잿빛곰팡이병균(病菌)의 발생(發生))

  • Yu, Seung Hun;Park, Jeong Hun
    • Korean Journal of Agricultural Science
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    • v.19 no.2
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    • pp.170-178
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    • 1992
  • Isolates of Botrytis cinerea resistant to dicarboximide fungicides were collected from strawberry fields in greenhouses in spring and early summer of 1990. Five out of 9 isolates of B. cinera were resistant, which showed mycerial growth on PDA containing dicarboximide fungicides(procymidone and vinclozolin) with concentrations of 100, 400 and $1,600{\mu}g/ml$. The minimal inhibitory concentration(MIC) values of the dicaboximide-resistant isolates was more than $6,400{\mu}g/ml$, while that of the sensitive isolates was less than $6.25{\mu}g/ml$. The germination ratio of conidia of the resistant isolates on PDA containing procymidone and vincolozolin was more than 95%, while that of the sensitive was less than 15%. The procymidone-resistant isolates were also resistant to vinclozolin, showing cross-resistant between the fungicides, but cross-resistant was not observed between the dicarboximides and dichlofluanid. Resistance to benomyl was also found in all the dicarboximide resistant isolates. Occurrence frequency of dicarboximide-resistant isolates out of 223 isolates was about 40%. The resistant isolates were widely distributed throughout Korea.

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