• Title/Summary/Keyword: Mutagensisi of protoplasts

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Isolation of Auxotrophic Mutants from Protoplasts of Pleurotus ostreatus and Pleurotus florida (자외선(紫外線) 조사(照射)에 의한 느타리버섯과 사철느타리버섯 원형질체(原形質體)의 영양요구성(營養要求性) 균주(菌株) 선발(選拔)에 관한 연구(硏究))

  • Yoo, Young-Bok;Peberdy, John F.;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.13 no.2
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    • pp.75-78
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    • 1985

  • Mutagenesis of protoplast could serve a great potential tool for improvement of strains and genetics in higher fungi. For the isolation of auxotrophic mutants from protoplasts of Pleurotus ostreatus and Pleurotus florida, viability levels of ultraviolet lights were determined. Seven auxotrophs were obtained from protoplasts irradiating UV to give $0.83{\sim}15%$ survival. The mutants showed a single requirement for each of Arg, Ribo-l, Ribo-2 or Phen for growth. Some of them showed two or three kinds of requirements, Gly Ser, Ade Hypo or Ala Om Tryp for growth.

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