• Mycobiology
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• v.47 no.1
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• pp.97-104
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• 2019

Mushroom cultivation has gained increased attention in recent years. Currently, only four types of spawn, including sawdust spawn, grain spawn, liquid spawn, and stick spawn, are commonly available for mushroom cultivation. This limited spawn diversity has led to difficulty in selecting suitable inoculum materials in some cultivation. In this study, three small blocks of lignocellulosic agro-wastes and one block of a synthetic matrix were prepared as support for growing Pleurotus ostreatus in liquid medium. Mycelium-adsorbed blocks were then evaluated for their potential as block spawn for fructification. Our results indicated that the edible fungus was adsorbed and abundantly grew internally and externally on loofah sponge and synthetic polyurethane foam (PUF) supports and also has the ability to attach and grow on the surface of sugarcane bagasse and corncob supports. The mycelia of P. ostreatus adhered on corncob exhibited the highest metabolic activity, while those on the PUF showed the least activity. Mycelial extension rates of block spawns made of agro-waste materials were comparable to that of sawdust spawn, but the block spawn of PUF showed a significantly lower rate. No significant differences in cropping time and yield were observed among cultivations between experimental block spawns and sawdust spawns. Moreover, the corncob block spawn maintained its fruiting potential during an examined period of 6-month storage. The developed block spawn could be practically applied in mushroom cultivation.

• The Korean Journal of Mycology
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• v.37 no.2
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• pp.144-149
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• 2009

The occurrences of the major diseases and the densities of air-born microbes were surveyed in the cultivation facilities for oyster mushroom (Pleurotus ostreatus), king oyster mushroom (Pleurotus eryngii), and enoki mushroom (Flammulina velutipes) in different areas of Korea. Green mold disease was most often developed in oyster mushroom bed cultivation with the disease incidence rate of approximate 10% while the disease incidences from bottle and plastic envelop cultivation were less than 1~2%. In the bed cultivation, the major air-born microbes in the growth room were Aspergillus, Penicillium, Trichoderma, and Curvularia with the total fungal population density of 567~1,297 CFU/$m^3$ . However, only Trichoderma and Penicillium were detected in the growth rooms and innoculation rooms of bottle and plastic envelop cultivation with the densities of 350~700 CFU/$m^3$ and 160~260 CFU/$m^3$, respectively. The bacterial diseases become evident in the growth rooms of bottle and plastic envelop cultivation with the approximate incidence rate of 10%. The identified bacterial species were Brevibacillus levelkil, Rhizobium radiobacter, Brevundimonas vesicularis, Pseudomonas mosselii, Microbacterium testaceum. Sphingomonas panmi, Sphingomonas yabuuchiae, Paracocus dinitrificans, Curtobacterium flaccumfaciens pv. flaccumfaciens and some unidentified bacteria with the densities of 40~6,359 CFU/$m^3$ in the growth rooms and 9 CFU/$m^3$ in the inoculation room. This study indicated that the green mold disease by fungal strains was the major mushroom disease in the bed cultivation and suggested that the contamination of bacteria and fungi together in the growth media could result in severe production loss. The plastic envelope and bottle cultivation were evidenced to be less susceptible to such contaminations.

• Journal of Mushroom
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• v.18 no.1
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• pp.91-94
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• 2020

This study was carried out to develop a method for cultivation of Lentinula edodes that would reduce both labor and waste. Cultural characteristics were studied, and mushroom yields were estimated to identify the most suitable method to culture L. edodes, using bottles and boxes. Among these, box cultivation with 7 kg of substrate had 40 days of browning period and the maximum yield of 945 g fruiting body; its biological efficiency was 32.7%. In contrast, box cultivation after pre-culture, and the bottle cultivation were found to be unsuitable for the production of L. edodes, due to long periods of browning, and low yields with poor quality, respectively. Further studies on box cultivation of L. edodes are necessary for commercial application of this method.

• Mycobiology
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• v.31 no.1
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• pp.50-53
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• 2003

Perforated vinyl mulching technique was performed on oyster mushroom beds for controlling mushroom diseases. Mycelium under vinyl sheets were safely protected from outside undesirable microorganisms. One of two mushroom farms showed 75% of disease incidence, the other 40% and National Institute of Agricultural Science and Technology(NIAST) 13% in the conventional growing method, whereas 12%, 14%, and 5% in the vinyl mulching cultivation method. Waterlogging caused mushroom bed worse, and Trichoderma spp. were infested on the conventional mushroom bed. Disease incidence investigated in other case was 25% to 30% in the conventional growing method, whereas 5 to 9% in the vinyl mulching cultivation method. Yields in conventional method were 6.5 to 7.2 $kg/m^2$ and those in vinyl mulching method were 7.6 to 8.1 $kg/m^2$. So it was suggested that vinyl mulching technique was good for prevention from disease and elevation of productivity.

• Journal of Mushroom
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• v.19 no.1
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• pp.66-70
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• 2021

We monitored the occurrence of fungal species on bed-logs inoculated with oak mushroom (Lentinula edodes) and the environmental conditions of temperature and humidity in the cultivation houses during monsoon. Six fungal species, viz., Cladosporium sp., C. cladosporioides, C. anthropophilum, Pleosporales sp., Trichoderma harzianum, and Acremonium sp., wereidentified from the cultivation houses located in Jangheung, Jeonnam province. This identification was confirmed by performing nucleotide sequence analysis of the internal transcribed spacer and 28S rDNA regions. Our study presents significant findings that can help in preventing fungal damage induced by inappropriate temperature and humidity in oak mushroom cultivation houses.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.41 no.3
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• pp.71-75
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• 2009

Cotton waste is usually used for cultivating agaric mushroom after outdoor fermentation for a few months. Electron beam was used to break down the polymer chaims of cotton waste for increasing low molecular weight soluble sugars, which may enhance the agaric mushroom cultivation. By increasing electron beam radiation, alpha cellulose content of the cotton waste was decreased while beta cellulose content and hot water solubles were increased. Electron beam radiation over 240 kGy on cotton waste caused significant increase of mushroom yield without lowering mushroom quality.

• Journal of Korean Society of Forest Science
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• v.63 no.1
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• pp.61-68
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• 1984

The oak-mushroom is one of the special minor products utilizing hardwoods, such as oaks in the mountaineous villages, as a food for health and export in Korea. However, oak-mushroom farmers have been faced the problems such as small cultivation scale, unskilled technique of production, management of cultivation, and low productivity. It is now a turning point presenting the optimum and reasonable management techniques to increase the productivity with consideration of balanced income and expenditure. This report analyzed the investment effectiveness of cultivation. The financial rate of return (FRR) of oak-mushroom cultivation is as high as 10.3 percent compared with that of other farmings. Moreover, the FRR remarkably increased with increased cultivation scales. The FRR could be highly improved if the price of both mushroom-logs and dried mushroom products, is stable and the cultivation techniques for higher productivity are developed. The policy providing the constant price of raw materials and mushroom products, and new advanced cultivation techniques enable the enhancement of investment for the oak-mushroom cultivation.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.13-13
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• 2014

Two kinds of mushrooms, Gumji (金芝; Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; B.C 57~A.D 668; written by Bu Sik Kim in 1145) in Korea-dynasty. Many kinds of mushrooms were also described in more than 17 kinds of old books during Chosun-dynasty (1392~1910) in Korea. Nowadays, mushroom cultivation has been increased through out the world last decade years. Production of mushrooms has also been increased 10-20% and many varieties have been cultivated. Similar trends were also observed in Korea. Approximately two hundred commercial strains of 37 species in mushrooms were developed and distributed to cultivators. Somatic hybrid variety of oyster mushroom 'Wonhyeong-neutari' were developed by protoplast fusion, and distributed to grower in 1989. The fruiting body yield index of somatic hybrids of Pleurotus ranged between 27 and 155 compared to parental values of 100 and 138. In addition, more diverse mushroom varieties such as Phellinus baumi, Auricularia spp., Pleurotus ferulae, Hericium erinaceus, Hypsizigus marmoreus, Grifola frondosa, Agrocybe aegerita and Pleurotus cornucopiae have been attempted to cultivate in small scale cultivation. Production of mushrooms as food was 190,111 metric tons valued at 800 billion Korean Won (one trillion won if include mushroom factory products; 1dollar = 1,040 Won) in 2011. Major cultivated species are Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Ganoderma lucidum, which cover 90% of total production. Since mushroom export was initiated from 1960 to 1980, the export and import of mushrooms have been increased in Korea. Technology developed for liquid spawn production and automatic cultivation systems lead to the reduction of the production cost resulting in the increasement of mushroom export. However some species were imported because of high production cost for these mushrooms requiring the effective cultivation methods. Developing of effective post-harvest system will be also directly related to mushroom export. In academic area, RDA scientists have been conducting mushroom genome projects. One of the main results is the whole genome sequencing of Flammulina velutipes for molecular breeding. An electrophoretic karyotype of of F. velutipes was obtained using CHEF with 7 chromosomes, with a total genome size of approximately 26.7 Mb. The mususcript of the genome of F. velutipes was published in PLOS ONE this year. For medicinal mushrooms, we have been conducting the genome research on Cordyceps and its related species for developing functional foods using this mushroom. In 2013, Korea Food and Drug Administraion (KFDA) approved Cordyceps mushroom for its value as an immune booster.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.125-129
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• 2010

This study was carried out to elucidate suitable spawn culture for an artificial cultivation of Neolentinus lepideus. The optimum culture conditions of the liquid spawn were deffated soy flour for main material, 12 days for culture period and 0.9 vvm for aeration volume, respectively. Sawdust spawn was appropriate for douglas fir sawdust and deffated corn flour (95 : 5, v/v) for mycelia growth and fruiting body formation. In case of liquid spawn, cultivation period was two shorter than sawdust spawn and mushroom yield was 111.9 g per 850ml cultivation bottle. In conclusion, the suitable spawn for fruiting body production was found to be liquid spawn than sawdust spawn with considering cultivation period and mushroom productivity.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.142-145
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• 2010

In this study, we attempted to find substitute materials, swelling rice hull, cocopeat, corncob and coconut sawdust, for sawdust in bottle cultivation of Pleurotus ostreatus. Chemical characters of mixture substrates with four substitute materials for pine sawdust were not different significantly. By comparison in mycelial growth and yield of fruitbody, mixture substrate of cocopeat were showed the same level in P. ostreatus. Therefore, it is suggested that cocopeat was substituted for sawdust for cultivation of P. ostreatus.