• Title/Summary/Keyword: Muscle characteristic

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Comparison of Pork Quality and Muscle Fiber Characteristics between Jeju Black Pig and Domesticated Pig Breeds (제주 개량 흑돼지와 국내에 도입된 주요 돼지 품종의 육질 특성 및 근섬유 특성 비교)

  • Ko, Kyong Bo;Kim, Gap-Don;Kang, Dong-Geun;Kim, Yeong-Hwa;Yang, Ik-Dong;Ryu, Youn-Chul
    • Journal of Animal Science and Technology
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    • v.55 no.5
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    • pp.467-473
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    • 2013
  • This study compared the pork quality and muscle fiber characteristics between the Jeju black pig (JBP) and domesticated pig breeds, including three-way crossbred (Landrace ${\times}$ Yorkshire ${\times}$ Duroc, LYD), Berkshire (B), Duroc (D), Landrace (L), Meishan (M), and Yorkshire (Y) pigs. JBP had the lowest carcass weight among the pig breeds (p<0.05) and its NPPC marbling score was lower than the other domesticated pig breeds (p<0.05), except for M. M had the highest value for backfat thickness among the pig breeds (p<0.05), whereas its NPPC marbling score was the lowest (p<0.05). The pH values were higher in JBP and B at 45 min and 24 h postmortem, respectively, but LYD and M had the lowest pH values at 45 min and 24 h postmortem, respectively (p<0.05). M had a higher lightness and lower redness than the other breeds (p<0.05). Moreover, M had the highest drip loss among the pig breeds (p<0.05), whereas D had the lowest drip loss and the highest redness (p<0.05). In terms of muscle fiber characteristics, JBP and LYD had the largest type IIB fibers (p<0.05). The fiber number and area composition of type I fibers were higher in JBP than the others (p<0.05) however type IIA fibers were lowest in JBP among the pig breeds (p<0.05). Overall, our results indicate that JBP provided better pork quality compared with M, but the carcass trait and pork quality of JBP were not better than LYD and D, respectively.

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

  • Ropka-Molik, Katarzyna;Bereta, Anna;Zukowski, Kacper;Tyra, Miroslaw;Piorkowska, Katarzyna;Zak, Grzegorz;Oczkowicz, Maria
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.10
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    • pp.1565-1574
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    • 2018
  • Objective: The aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants' effect on histological microstructure and production traits in a larger pig population. Methods: RNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits. Results: Mapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin 'eye' area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism. Conclusion: This study's results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

Physicochemical characteristics and fatty acid profiles of muscle tissues from Hanwoo steers fed a total mixed ration supplied with medicinal plant by-products

  • Lee, Shin Ja;Kim, Do Hyung;Yang, Han Sul;Nam, Ki Chang;Ahn, Seung Kyu;Park, Sung Kwon;Choi, Chang Weon;Lee, Sung Sill
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.10
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    • pp.1388-1395
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    • 2017
  • Objective: Using medicinal plant by-products (MPBP) as feed additives may be an eco-friendly option as substitutes for feedstuffs and may assist in reducing the improper disposal of MPBP. Therefore, this study was conducted to evaluate the influences of MPBP on the meat quality of Hanwoo steers fed a total mixed ration (TMR). Methods: Twenty seven steers (body weight = $573{\pm}57kg$) were randomly divided into three treatments with a control group and two tested groups as follows: control, 1,000 g/kg TMR; treatment 1 (MPBP30), 970 g/kg TMR and 30 g/kg MPBP; treatment 2 (MPBP50), 950 g/kg TMR and 50 g/kg MPBP. Results: Average daily gain, feed conversion ratio and the Commission Internationale de l'Eclairage $L^*$ of muscle were improved (p<0.05, respectively) by MPBP30. Stearic acid ($C_{18:0}$) was decreased (linear effect, p = 0.012), while oleic acid ($C_{18:1}$) was increased (linear effect, p = 0.055) by MPBP level. Saturated fatty acid (SFA) and polyunsaturated fatty acid (PUFA) were decreased for MPBP50 while unsaturated fatty acid (USFA) and monounsaturated fatty acid (MUFA) were increased for MPBP 50. USFA and SFA ratio was increased for MPBP50 as well. Conclusion: These results indicated that MPBP supplementation in Hanwoo steers fed a TMR increased feed efficiency and meat color (lightness) with altering fatty acid proportions. Therefore, MPBP may be successfully used in ruminant feeding.

Anatomical Study of Chordodes koreensis in the Parasitic Phase Using Electron Microscopy (개의 구토물에서 분리한 오디흑연가시(철선충)의 전자현미경을 이용한 해부학적 소견)

  • Son, Hwa-Young;Chae, Joon-Seok;Kim, Nam-Soo;Kim, Hyeon-Cheol;Cho, Jeong-Gon;Park, Bae-Keun
    • Journal of Veterinary Clinics
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    • v.26 no.6
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    • pp.586-590
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    • 2009
  • Horsehair worms (Chordodes koreensis) develop as parasites in the bodies of grasshoppers, crickets, cockroaches, and some beetles. Chordodes koreensis is an accidental parasite of humans, livestock, or pets and poses no public health threat. The male of Chordodes koreensis in the later larval stage from canine vomitus was investigated by the scanning and transmission electron microscopy. In cross sections, the body wall is composed of four components namely epicuticle, cuticle, epidermis, and muscle layers. The parenchymal tissue fills the rest of the body and surrounds the visceral organs such as intestine, and ventral nerve cord but testes were not found. The epicuticle is a thin superficial layer whose surface shows rows of polygonal elevations called areoles. The cuticle has 17 layers of collagenous fibers spirally wound about the long axis of the worm. The section through the cuticle reveals the layers of large fibers cut obliquely lengthwise, alternating with layers of fibers sectioned obliquely crosswise. The layers of large fiber formed a double helix about longitudinal axis of the worm. The epidermis is a single layer. The muscles were interrupted by the nervous lamella in the only midventral portion. The medulla of muscle plate is composed of lightly stained cytoplasm, mitochondria, weakly developed endoplasmic reticulum, and glycogen granules. Between the medulla of a cell and the plasmalemma lies a broad cortical zone of myofilaments. The circular muscles are absent. The characteristic feature of the cytoplasm is that there was no content in peripheral mesenchyme, but was an abundance of large clear vacuoles which give the cytosome a foamy appearance. The nucleus of mesenchyme is not easily identified in our specimens.

Influence of Spices on Histological Characteristic of Beef (향신료(香辛料)의 처리(處理)가 우육(牛肉)의 조직학적(組織學的) 특성(特性)에 미치는 영향(影響))

  • Chung, Byung-Sun;Lee, Yong-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.3
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    • pp.11-20
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    • 1987
  • The study was carried out to observe some fundamental effect of spices on tenderization of beef, particularly round muscle part. The study has been investigated analytically in terms of histological and sensory test to compare the tenderizing effect of the spices with respective effect of commercial meat tenderizer and mechanical tenderizer on beef. The results of formal titration assay using casein as a substrate were that garlic, radish and ginger were stronger in protein hydrolysis than the other spices. Beef with spice treatment produced partial degradation of muscle fiber and connective tissue. Connective tissues and muscle fiber were generally degraded conspicuously by the treatment of commercial meat tenderizer. A general disruption and severing of muscle fibers and severing of connective tissue were seen in the area of blade penetration. The results of sensory test on the texture were that F-value of 11.27 is significant at the 1% of the sample. Beef treated with spices was significantly tenderer than beef without treatment at 5% level.

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Interstitial Lung Disease Associated with Polymyositis: Response to Cyclophosphamide and Prednisolone Combination Treatment (Cyclophosphamide와 Prednisolone 병합요법에 치료반응을 보인 다발성근염에 동반된 간질성폐질환 1예)

  • Moon, Jong-Ho;Park, Jun-Young;Lee, Sang-Moo;Kim, Hyeon-Tae;Uh, Soo-Taek;Chung, Yeon-Tae;Kim, Yong-Hoon;Park, Choon-Sik;Lee, Kyung-Soo;Kang, Dae-Young
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.2
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    • pp.197-202
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    • 1993
  • Polymyositis (PM) is a inflammatory connective tissue disease involving predominantly skeletal muscles, characterized by symmetrical, proximal muscle weakness, inflammation, and frequently, degeneration. Interstitial lung disease in association with PM occurs in 5~10% of cases and carries an especially grave prognosis. Although the cause of lung involvement in PM is not known, the underlying pathologic process in the lung is an immune mediated inflammation of alveolar structures, alveolitis. It is of interest, therefore, that cyclophosphamide, an immune modulating agent, has been reported to be effective in the treatment of PM. We report a case of corticosteroid resistant PM associated with interstitial lung disease, successfully treated with cyclophosphamide. A 37-year-old female was presented with 8 months duration of cough, exertional dyspnea, and muscle weakness. She had typical symptoms, physical findings, and elevated muscle enzyme levels in serum with characteristic findings of muscle biopsy. She also had typical interstitial lung disease pattern on chest X-ray and high resolution CT with restrictive pattern on pulmonary function test. The findings of transbronchial lung biopsy was compatible with interstitial lung disease. She failed to respond to corticosteroid initially. Subsequently steroids and cyclophosphamide were given with excellent clinical improvement.

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Studies on the Bladder Worm, Cysticercus cellulosae -The Ulrastructure of C. cellulosae- (유구낭모충에 관한 연구 -유구낭모충의 미세구조-)

  • 이순형
    • Parasites, Hosts and Diseases
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    • v.21 no.1
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    • pp.75-82
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    • 1983
  • An electron microscopic study was performed to know the basic tegumental structure of Cysticercus cellulosae. The scolex and bladder portions of cysticerci (human and porcine strains) were prepared for transmission and scanning electron microscopy by conventional procedures. In general, the tegument of C. cellulosae showed the basic ultrastructure of cestode tegument on electron micrographs. The teguments of both scolex and bladder portions consisted of such components, i.e., an outer vesicular layer with numerous microtriches and inner filroug layer. Below the fibrous layer, there were layers of muscle bundles and tegumental cells. The microtriches which covered the surface of cysticercus revealed two distinctly different shapes. The characteristic bladder-like, elongated pyramid shaped "tetrahedrial form" was observed on the surface of the scolex portion, whereas the elongated cylindrical "filamentous form" was distributed on the stirface of bladder portion. In spite of the difference of isolated host and location, the cysticerci showed tole same result. But dimensional variations of the tegument according to topography of the worm were observed. The possibility of application in making differential diagnosis from other larval cestodes and possible functions of this larval tegument were discussed.

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Misunderstanding of Foot Drop in a Patient with Charcot-Marie-Tooth Disease and Lumbar Disk Herniation

  • Han, Youngmin;Kim, Kyoung-Tae;Cho, Dae-Chul;Sung, Joo-Kyung
    • Journal of Korean Neurosurgical Society
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    • v.57 no.4
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    • pp.295-297
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    • 2015
  • We report the case of 57-year-old woman diagnosed with Charcot-Marie-Tooth (CMT) disease and lumbar disk herniation (LDH). She had left leg weakness and foot numbness, foot deformity (muscle atrophy, high arch, and clawed toes). The lumbar spine MRI showed LDH at L4-5. Additionally, electrophysiology results were consistent with chronic peripheral motor-sensory polyneuropathy (axonopathy). In genetic testing, 17p11.2-p12 duplication/deletions characteristic of CMT disease were observed. We confirmed the patient's diagnosis as CMT disease and used conservative treatment.

Artifacts characteristic analysis of EEG (EEG의 잡파 특성 분석)

  • 양은주;조한범;김응수
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2002.05a
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    • pp.87-90
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    • 2002
  • 뇌파(Electroencephalogram, EEG)는 뇌 신경세포가 정보를 처리하는 과정에서 발생하는 전기적인 신호를 두피 표면에서 측정한 것이다. 이러한 뇌파는 비침습적인 방법으로 전기적인 신호를 측정하며 측정시 여러 잡파(artifact)가 섞이기 쉽다. 이러한 잡파는 뇌의 정보처리과정에 대한 유용한 정보를 담고 있는 뇌파를 분석하는데 방해가 되므로 이를 제거하기 위한 노력이 계속되어 왔다. 그러나 본 연구에서는 보다 적극적인 방향으로 잡파가 섞인 뇌파의 특성을 분석하여 이를 통해 제어 시스템 등과 같은 시스템에 적용할 수 있는 가능성을 알아보았다. 대표적인 잡파인 eye_blinking, eye_rolling, muscle 등이 각각 포함된 뇌파에 대해서 선형 및 비선형 분석을 실시함으로써 유의미한 특성 차이를 나타내었다.

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Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia in a Bengal Cat

  • Cho, Mun-Ju;Kim, Myung-Chul;Seo, Kyoung-Won
    • Journal of Veterinary Clinics
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    • v.34 no.6
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    • pp.481-483
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    • 2017
  • A 2-year-old neutered male Bengal cat presented with a 6-month history of weight loss and chronic vomiting. An abdominal ultrasound revealed increased thickness of the pylorus and ascending duodenum with concurrent enlargement of the mesenteric lymph nodes. Histologically, branching and anastomosing trabecular pattern of dense collagen was the characteristic feature, and large populations of spindle cells were also observed. These large spindle-shaped cells were positive for smooth muscle actin and vimentin on immunohistochemical examination. Based on these findings, the cat was diagnosed with feline gastrointestinal eosinophilic slcerosing fibroplasia (FGESF), and immunosuppressive therapy was initiated immediately. Unfortunately, the cat's condition deteriorated despite treatment; the cat died 56 days after initiation of therapy. This is the first report of FGESF in South Korea.