Anatomical Study of Chordodes koreensis in the Parasitic Phase Using Electron Microscopy

개의 구토물에서 분리한 오디흑연가시(철선충)의 전자현미경을 이용한 해부학적 소견

  • Son, Hwa-Young (Research Institute of Veterinary Medicine.College of Veterinary Medicine, Chungnam National University) ;
  • Chae, Joon-Seok (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Nam-Soo (Research Institute of Veterinary Medicine.College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Hyeon-Cheol (School of Veterinary Medicine, Kangwon National University) ;
  • Cho, Jeong-Gon (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Park, Bae-Keun (Research Institute of Veterinary Medicine.College of Veterinary Medicine, Chungnam National University)
  • 손화영 (충남대학교 수의과대학.동물의과학연구소) ;
  • 채준석 (서울대학교 수의과대학) ;
  • 김남수 (충남대학교 수의과대학.동물의과학연구소) ;
  • 김현철 (강원대학교 수의과대학) ;
  • 조정곤 (전북대학교 수의과대학) ;
  • 박배근 (충남대학교 수의과대학.동물의과학연구소)
  • Published : 2009.12.31

Abstract

Horsehair worms (Chordodes koreensis) develop as parasites in the bodies of grasshoppers, crickets, cockroaches, and some beetles. Chordodes koreensis is an accidental parasite of humans, livestock, or pets and poses no public health threat. The male of Chordodes koreensis in the later larval stage from canine vomitus was investigated by the scanning and transmission electron microscopy. In cross sections, the body wall is composed of four components namely epicuticle, cuticle, epidermis, and muscle layers. The parenchymal tissue fills the rest of the body and surrounds the visceral organs such as intestine, and ventral nerve cord but testes were not found. The epicuticle is a thin superficial layer whose surface shows rows of polygonal elevations called areoles. The cuticle has 17 layers of collagenous fibers spirally wound about the long axis of the worm. The section through the cuticle reveals the layers of large fibers cut obliquely lengthwise, alternating with layers of fibers sectioned obliquely crosswise. The layers of large fiber formed a double helix about longitudinal axis of the worm. The epidermis is a single layer. The muscles were interrupted by the nervous lamella in the only midventral portion. The medulla of muscle plate is composed of lightly stained cytoplasm, mitochondria, weakly developed endoplasmic reticulum, and glycogen granules. Between the medulla of a cell and the plasmalemma lies a broad cortical zone of myofilaments. The circular muscles are absent. The characteristic feature of the cytoplasm is that there was no content in peripheral mesenchyme, but was an abundance of large clear vacuoles which give the cytosome a foamy appearance. The nucleus of mesenchyme is not easily identified in our specimens.

철선충은 주로 사마귀와 같은 곤충에 기생하는 선충류로 사람이나 동물은 우연히 감염될 수 있는 것으로 알려져 왔다. 철선충의 생활사는 크게 곤충에 기생하는 기생생활기, 그리고 발육이 완료된 후에 숙주를 죽이고 외계로 나와 자유생활을 하는 자유생활기로 구분할 수 있다. 본 연구는 개의 구토물에서 오디흑연가시 수컷을 발견하여 이의 횡단면을 주사 및 투과 전자현미경으로 관찰한 것이다. 전자현미경하에서 표피는 4층(epicuticle, cuticle, epidermis, muscle)으로 이루어졌고, 내강에는 장, 배신경절이 발견되었다. 그러나 고환은 발견되지 않았다. 표피의 최상층에서 여러 형태의 areoles이 관찰되었으며, cuticle은 17열의 섬유로 이루어져 있었다. Epidermis는 1열로 구성되어 있었으며, muscle층은 여러 개의 근세포가 종으로 일정하게 배열되어 있었다. 그리고 내강에 배신경절이 충체의 정중앙 배측에서만 존재하는 것으로 확인되었다. 이러한 결과로 본 철선충은 기생생활기를 끝내지 못한 미성숙충으로 판단된다.

Keywords

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