• Asian-Australasian Journal of Animal Sciences
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• 제32권4호
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• pp.592-598
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• 2019

Objective: Autophagy is a bulk degradation system for intracellular proteins which contributes to skeletal muscle homeostasis, according to previous studies in humans and rodents. However, there is a lack of information on the physiological role of autophagy in the skeletal muscle of meat animals. This study was planned as a pilot study to investigate changes in expression of two major autophagy-related genes, microtubule-associated protein 1 light chain $3{\beta}$ (MAP1LC3B) and autophagy related 7 (ATG7) in fattening beef cattle, and to compare them with skeletal muscle growth. Methods: Six castrated Japanese Black cattle (initial body weight: $503{\pm}20kg$) were enrolled in this study and fattened for 7 months. Three skeletal muscles, M. longissimus, M. gluteus medius, and M. semimembranosus, were collected by needle biopsy three times during the observation period, and mRNA levels of MAP1LC3B and ATG7 were determined by quantitative reverse-transcription polymerase chain reaction. The expression levels of genes associated with the ubiquitin-proteasome system, another proteolytic mechanism, were also analyzed for comparison with autophagy-related genes. In addition, ultrasonic scanning was repeatedly performed to measure M. longissimus area as an index of muscle growth. Results: Our results showed that both MAP1LC3B and ATG7 expression increased over the observation period in all three skeletal muscles. Interestingly, the increase in expression of these two genes in M. longissimus was highly correlated with ultrasonic M. longissimus area and body weight. On the other hand, the expression of genes associated with the ubiquitin-proteasome system was unchanged during the same period. Conclusion: These findings suggest that autophagy plays an important role in the growth of skeletal muscle of fattening beef cattle and imply that autophagic activity affects meat productivity.

• 대한한방소아과학회지
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• 제28권2호
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• pp.1-22
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• 2014

Objectives The purpose of this study is to investigate the relationship between weak children and growth indices. Methods One hundred twenty-six of the first and the second grade elementary school students who didn't have to develop yet their secondary sexual characteristics answered the questionnaire. Their bone age and body composition were measured. Results 1. According to the Weak Children questionnaire analysis, respiratory problem was one of the biggest problems in the weak children group (35.7%), followed by digestive problems (23.0%), psycho-neurological problems (22.2%), neuromotor problems (9.5%), and urogenital problems (8.7%). 2. From the comparison between growth indices of weak children and that of normal children are as follows: 1) The group of children who had problem in their digestive system had lower growth indices than the normal average children group. The growth indices includes mean height, weight, total body water, protein mass, mineral mass, body fat, skeletal muscle mass, body fat percentage, body mass index (BMI), and basal metabolic rate (BMR). 2) The children who had urogenital weakness had lower mean height, RH (Recent Height), RH - MPH {Recent Height(%) - Mid-Parental Height(%)}, RUS (Radius, Ulna and Short bone) score, weight, protein mass, body fat, BMI, and visceral fat than the normal children group. 3. The results of the multiple comparisons of growth indices and weak children groups are as follows: 1) Digestive weak children were the lowest in total body water, protein mass, mineral mass, skeletal muscle mass, and basal metabolic rate. 2) Urogenital weak children were the lowest in height, RH, RH - MPH, RUS score, and weight. Conclusion These results showed that children's growth is strongly correlated to their own growth problems, especially to those children who have digestive and urogenital problems. Therefore, this may be an effective way for children growth treatment in Korean medicine to treat weak symptoms.

• 생명과학회지
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• 제29권11호
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• pp.1235-1240
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• 2019

본 연구에서는 발효어분이 넙치의 증체율에 미친 조직학적 영향을 규명하기 위하여 수입어분에 대한 발효어분의 대체비율 0, 25, 50, 75 및 100%로 사육한 넙치로부터 간 및 근육을 적출하여 광학현미경을 이용한 미세구조적 분석을 실시하였다. 그 결과, 25% 대체군에서 간중량지수가 가장 높고 간 지방함유율이 가장 낮은 점으로부터 25% 대체군의 간의 우수한 건강성이 시사되었다. 또한 근섬유의 단면적도 25% 대체군이 가장 넓게 나타나, 운동성 또한 우수한 것으로 추측되었다. 즉 간 및 근육의 우수한 건강 상태가 높은 증체율을 나타내는데 영향을 미쳤음을 알 수 있었다. 한편, 증체율이 낮은 75% 및 100% 대체군에서는 간건강 및 근섬유의 발달이 좋지 못했는데, 이는 발효어분이 일정 비율 이상 포함될 경우 넙치의 건강에 좋지 않은 영향을 미칠 수 있음이 시사되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.562-566
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• 2005

This study was conducted to investigate the developmental relationship between fatty acid composition in different lipid fractions and stearoyl-CoA desaturase (SCD) gene expression in steer muscles during growth. Twenty Hanwoo steers were used at 6, 12, 18, 24 and 30 months of age. Fatty acid composition and SCD mRNA level were analyzed. In the total lipid fraction, developmental profiles of C18:1, as the product of SCD enzyme, and SCD mRNA level were significantly increased between 6 months and 12 months of age. During this period, the percentage of C18:1 increased from 31.9% to 49.5% in the total lipid. The increased C18:1 level was maintained until 30 months of age within the range of 44.8- 49.9%. In contrast, the C18:0 composition decreased with age and this decrease was compensated by the increase of the C18:1. However, the sum of C18:0 and C18:1 was changed before and after 12-month old by a 20% increase. Unlike the C18 fatty acids, the C16 fatty acids such as C16:0 and C16:1 did not show a consistent change with age in steers' muscle. On the other hand, C18:2 proportion as a major polyunsaturated fatty acid in muscle was significantly reduced from 21.1% at 6 months of age to 4.4% at 12-months old and then this reduced level was maintained until 30 months within the range of 7.4-11.4%. As in the C18:1 composition during early stages, a 2-fold significant increase was observed in the $\Delta^9$-desaturase index of C18 fatty acid as a measure of SCD activity, but not in that of C16 fatty acid. Also, the steady-state level of SCD mRNA reached a peak at 12 months of age. Thus, the positive relationship between the C18:1 composition and the $\Delta^9$-desaturase (SCD enzyme) index of C18 fatty acid or SCD mRNA level was demonstrated during growth, but the negative relationship between the C18:2 composition and the above three indices was demonstrated at the same time, indicating that the sharp induction of SCD mRNA may be closely related to the dramatic reduction of C18:2, which is known as a suppressor of SCD gene expression during growth.

• 수산해양교육연구
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• 제27권2호
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• pp.390-398
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• 2015

A feeding experiment was conducted to determine the use of distillers dried grain (DDG) as a partial replacement for fish meal in the diet for juvenile rockfish, Sebastes schlegeli. Four iso-nitrogenous (50% crude protein) and iso-caloric (4.3 kcal/g) diets (designated as DDG0, DDG7, DDG14, and DDG21) were formulated to contain 0, 7, 14, and 21% DDG. Triplicate groups of juvenile rockfish (initial body weight, $10.2{\pm}0.2g$) were fed one of the experimental diets to visual satiety twice a day (09:00 and 17:00) for 8 weeks. At the end of the feeding trial, survival of rockfish was above 97% and not affected by dietary DDG levels (P>0.05). Weight gain, feed efficiency and daily feed intake of juvenile rockfish were significantly decreased with increase of dietary DDG levels (P<0.05). Condition factor, hepatosomatic index and visceralsomatic index of juvenile rockfish were not significantly affected by dietary DDG levels (P>0.05). No significant differences were observed in the contents of moisture, crude protein, crude lipid and ash of the whole body and dorsal muscle in juvenile rockfish fed the experimental diets (P>0.05). Therefore dietary inclusion of DDG as a replacement for fish meal could depress the growth of juvenile rockfish.

• Journal of Nutrition and Health
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• 제30권1호
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• pp.3-11
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• 1997

This study was conducted to investigate the effects of protein levels and casein/whey ratios on organ growth and protein metabolism in early weaned rats. Premature rats weaned by the 17th day were fed six semipurified synthetic, isocaloric and gel diets that contained three levels (low, medium and high) and two different combinations(casein/whey ; 80 : 20 or 20 : 80) of milk protein for 8 days. On the 25th day postpartum, frest weigth and DNA, RNA and milk protein contents in brain, liver, kidney and muscle were determined to ascertain organ and cellular growth. Futher, with a view to ascertain protein metabolism and renal functions, serum total protein, $\alpha$-amino N, urea N, and creatinine and creatinine and urinary urea N, creatinine and hydroxproline were determined. Total DNA contents of brain, liver and kidney, which may represent as an index of cell numbers in those organs were significantly decreased in the rats fed diets containing low level protein regardless of casein/whey ratio. However, as fat as the rats fed high protein diets were concerned, their fresh weight, protein contents and GFR of kidney were significantly increased. Furthermore, nitrogen components, $\alpha$-amino N, urea N and creatinie in serum and urine were also increassed. Another observation was that high casein/whey ratio significantly facilitated accumulation of porteins in muscle and kidney and urinary hydorxyproline excretion, not affecting the DNA content of those organs. This study showed that low(8%) or high(32%) contents of protein had less desirable effects either on protein metabolism or on organ cellular growth in prematurely weaned rats, whereas there were no effects on general growth and bone strength.

• 한국응용과학기술학회지
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• 제37권6호
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• pp.1646-1658
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• 2020

이 연구의 목적은 운동 후 수중회복방법의 차이가 성장호르몬, 테스토스테론, 혈중젖산농도, 심부담도, 통증의 변화를 알아보는 것이다. 저항운동 경력 6개월 이상인 남자 7명을 대상으로 집중 웨이트 트레이닝과 서킷 웨이트트레이닝을 1RM의 60%강도로 실시한 다음 회복방법으로 수중걷기회복과 수중앉기 회복을 진행하였다. 성장호르몬은 모든 운동형태와 회복방법에서 운동 후, 회복 후, 안정 시 순으로 높게 나타났으며, 테스토스테론은 운동 후, 회복 후, 안정 시 순으로 높았다. 혈중젖산농도는 모든 운동형태와 회복방법에서 운동 후, 회복 후, 안정 시 순으로 높았으며 집중저항운동 후 동적회복이 정적회복보다 낮았다. 심부담도는 모든 운동형태와 회복방법에서 운동 후, 회복 후, 안정 시 순으로 높았다. 근통증은 모든 운동형태와 회복방법에서 운동직후, 회복 후, 24시간, 48시간, 72시간 순으로 감소하였다. 물속 환경에서도 동적회복이 정적회복보다 근피로 개선에 효과가 있는 것으로 사료된다.

• 한국수산과학회지
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• 제45권2호
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• pp.97-103
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• 2012

This study compared the composition and texture of muscle and levels of detoxification and antioxidant enzymes in the livers of stocked and cultivated rockfish Sebastes schlegeli released after the primary culture stage in Tongyoung, South Korea. The crude lipid content of muscle was significantly higher ($P$<0.05) in cultivated rockfish than stocked rockfish, while the texture did not differ significantly ($P$>0.05). The condition factor (CF) and hepatosomatic index (HSI) did not differ significantly and the growth of stocked and cultivated rockfish was similar. The levels of the detoxification enzymes cytochrome P450 (CYP) and Ethoxyresorufin-O-deethylase (EROD) were significantly lower in the livers of stocked rockfish, perhaps because of their reduced exposure to xenobiotic compounds. In addition, stocked rockfish had a significantly ($P$<0.05) lower CAT and higher GR than cultivated rockfish, but similar levels of tGPx, SOD, GSH, and GSSG. The present study shows that the growth rates of stocked and cultivated rockfish are similar and that stocked rockfish are exposed to fewer xenobiotic compounds and less oxidative stress.

• 대한인간공학회:학술대회논문집
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• 대한인간공학회 1997년도 추계학술대회논문집
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• pp.406-413
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• 1997

근전도(EMG:Electromyogram)를 사용하여 국부 근육 피로(Localized Muscle Fatigue)를 정량화으로 분석 하기 위해 널리 이용되고 있는 AR(Autoregressive)모델의 1차 계수, RMS(Root Mean Square), ZCR(Zero Crossing Rate), MPF(Mean Power Frequency), MF(Median Frequency)를 선택하여, 근육이 발휘하는 힘과 시간의 흐름에 따라 근육 피로의 정도를 민감하게 나타내는 매개변수를 규명하였다. 피실험자 10명의 좌우 척추세움근(Erector Spinae Muscle)을 대상으로 등장수축(Sustained Isometric Contraction)조건에서 허리의 신전(Extension)운동을 실시하였다. 이때 발휘해야 하는 힘의 수준은 15%, 30%, 45%, 60%, 75% MVC 로 정하였고 각 수준마다 20초 동안 근전도를 측정하 였다. 데이터 분석은 총20초 구간의 근전도를 0.5초 간격으로 나누어 매개변수들을 각각 구하고 분석을 실시하였다. 시간의 흐름에 대한 피로도 분석 결과, AR 모델의 1차 계수와 MPF가 유의한 차이를 보였으며, 낮은 수준의 %MVC에서는 AR 계수가, 높은 수준에서는 MPF가 민감한 반응 결과를 나타냈다. 그리고 근육이 발휘하는 힘의 정도를 분석하기 위해 주로 사용되고 있는 RMS 보다는 더 AR 계수가 모든 수준에서 뚜렷하게 차이를 보인 것이 확인되었다. 따라서 AR 모델의 1차 계수가 근육의 피로 정도와 힘의 수준을 다른 매개변수에 비해 더욱 민감하게 구별함이 입증되었다. 이러한 결과는 다른 분야에서도 근육 피로를 정량적으로 측정하는데 사용될 수 있을 것으로 생각되며, 개인적 변이도를 고려한 확률 기법을 사용한다면 보다 정확한 근전도 분석이 이루어질 것으로 기대된다.있음을 알 수 있었다. 사료된다.의 결과는 자전거 에르고노미터의 결과가 트레드밀의 결과에 87.60%정도 나타났다.음을 관찰하였다. 특히 vitamin C와 E의 병용투여는 상승적으로 적용하여 간세포손상을 더욱 억제시킴을 알 수 있었다.mance and on TFP(Total Factor Productivity) growth which is a pure measure of firm performance. To utilize the advantage of panel data, FEM(Fixed Effect Model) and REM(Random Effect Model) were used. The empirical result shows that the entropy index as a measurement of inter-business relatedness is not significant but technological relatedness index is significant. OLS estimates on pooled data were considerably different from FEM or REM estimates on panel data. By introducing interaction effect among the three variables for business portfolio properties, we obtained three findings. First, only VI (Vertical integration) has a significant positive correlation with ROS. Second, when using TFP growth as an dependent variable, both TR(Technological Relatedness) and f[ are signif

• Journal of Animal Science and Technology
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• 제49권1호
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• pp.51-58
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• 2007

본 연구는 fish oil의 첨가가 한우거세우의 증체, 배최장근의 지방산 조성 및 도체등급에 미치는 영향을 구명하고자 평균체중 440.8±32.4kg의 한우거세우 20두를 공시하여 실시하였다. 시험구 배치는 fish oil을 첨가하지 않는 FO-0구와 fish oil을 3% 첨가하는 FO-3구의 2처리로 하였다. 총 증체량과 일당증체량은 fish oil의 첨가에 관계없이 비슷한 결과를 보였다. Fish oil의 첨가는 배최장근의 단백질, 지방 및 회분 함량에 영향을 미치지 않았다. 배최장근의 isoleucine 및 glycine 함량은 fish oil의 첨가로 감소되었으나(p<0.05), cystein 함량은 증가되었다(p<0.05). Fish oil의 첨가는 배최장근의 myristic acid 및 eicosenoic acid 함량은 감소시켰으나(p<0.05), oleic acid 및 arachidonic acid 함량은 증가시켰다(p<0.05). 또한 배최장근의 EPA 및 DHA 함량은 fish oil의 첨가로 증가되었다(p<0.05). 도체중, 등지방두께, 배최장근단면적, 육량지수 및 육량등급은 fish oil의 첨가에 관계없이 두 처리구간에 유사한 경향을 보였다. 육색은 fish oil의 첨가로 개선되었으며 (p<0.05), 육질 1등급 이상 출현두수는 fish oil의 첨가로 증가하는 결과를 보였다. 따라서 본 연구의 결과에서 한우거세우에 대한 fish oil의 첨가는 쇠고기의 지방산 중에서 풍미 및 기호성에 관여하는 oleic acid 함량, 인간의 건강과 관련이 있는 EPA 및 DHA 함량 및 육질 1등급 이상 출현두수를 증가시키는데 효과적인 것으로 나타났다.