• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1451-1456
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• 2005

To study the acute effect of dietary docosahexaenoic acid (DHA, $C_{22:6}$) on the expression of adipocyte determination and differentiation-dependent factor 1 (ADD1) mRNA in pig tissues, weaned, crossbred pigs (28 d of age) were fed with either 10% (on as-fed basis) tallow (high stearic acid), soybean oil (high linoleic acid), or high DHA algal oil for 2 d. The plasma and liver DHA reflected the composition of the diet. The adipose tissue and skeletal muscle DHA did not reflect the diet in the short term feeding. The results also showed that the diet containing 10% algal DHA oil significantly decreased the total plasma cholesterol (39%) and triacylglycerol (TG; 46%) in the pigs. Soybean oil significantly decreased plasma TG (13.7%; p<0.05), but did not have an effect on plasma cholesterol. The data indicate that different dietary fatty acid compositions have different effects on plasma lipids. The ADD1 mRNA was decreased (p<0.05) in the liver of DHA oil-treated pigs compared with the tallow-treated pigs. The diets did not have significant effect on the ADD1 mRNA in adipose tissue. Addition of algal DHA oil in the diet increased acyl CoA oxidase (ACO) mRNA concentration in the liver, suggesting that dietary DHA treatment increases peroxisomal fatty acid oxidation in the liver. However, dietary soybean oil supplementation did not affect mRNA concentrations of ADD1 or ACO in the tissues of pigs. Because ADD1 increases the expression of genes associated with lipogenesis, and ACO is able to promote fatty acid oxidation, feeding DHA oil may change the utilization of fatty acids through changing the expression of ADD1 and ACO. Therefore, feeding pigs with high DHA may lead to lower body fat deposition.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1294-1298
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• 2005

The objective of the present study was to investigate the effects on growth performance, meat quality parameters and fatty acids of meat by restricted diet amount on finishing Berkshires. A total of 180 pigs (Berkshire, initially 52 kg BW) at 100 days of age were allotted in arrangement in a completely randomized design (10 pigs per pen), blocked arrangement of treatments with 3 replications. The variables were market ages (180, 200 and 220 days) and in which was also included sex (gilts and barrow). All the pigs were restrictively fed so that day could be marked at 103 kg. Pork quality was evaluated from 4 pigs of each treatment. Average daily gain (ADG) and average daily feed intake (ADFI) were decreased (p<0.05) with age increase. The ratio of feed to gain was increased (p<0.05) at 200 and 220 days compared to that of 180 days. Moisture and crude protein of longissimus dorsi muscle (LM) at 180 days were increased (p<0.05) compared to 200 and 220 days. Crude fat was increased (p<0.05) by age and crude ash was lower (p<0.05) at 180 days than at 220 days. Red to green meat color scale (CIE a$^{*}$) increased (p<0.05) at 200 and 220 days more than at 180 days. Regarding fatty acid composition in meat, saturated fatty acids (SFA) was increased more (p<0.05) at 220 days than at 180 and 200 days. The results indicate that even with a restricted diet of low nutrient supplement, there was an improvement in Berkshire meat quality parameters.

• Asian-Australasian Journal of Animal Sciences
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• 제12권8호
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• pp.1246-1250
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• 1999

Rabbits (48) of Soviet chinchilla (24) and White giant (24) were fed from 6 weeks to 12 weeks of age intensively on either of four isonitrogenous - isocaloric diets containing 0 ($D_1$), 5($D_2$), 10($D_3$) and 20($D_4$) percent raw neem seed kernel cake (NSKC), respectively as per NRC (1977) requirements in a Randomized block design and slaughtered at the end to find out differences in their carcass traits due to NSKC feeding. Dietary treatment had no significant effect on weight of edibles and inedibles and their percentages and dressing percentage in terms of carcass, carcass with pluck and carcass with pluck and head. Similarly, the meat-bone ratio of various primal cuts and overall carcass, yield of edibles per unit of inedibles and eye muscle area were not influenced due to the dietary variations. Chemical composition of fresh meat, and organoleptic evaluation of cooked meat with and without salt did not vary significantly due to incorporation of NSKC in the diets. The rabbits fed 20% NSKC ($D_4$) though consumed more (p<0.05) DM and DE per kg meat production, the intake of crude protein and total digestible nutrients was similar with other dietary treatments. Feed cost per unit meat production was, however, lower on 5 and 10% NSKC containing diets by 7.75 and 12.56%, respectively, as compared to deoiled ground nut cake containing control diet. It appears that NSKC could be used as a wholesome vegetable protein supplement upto 10% in diet of rabbits without any adverse effect on commercial carcass traits.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.113-118
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• 1999

The insulin-like growth factor (IGF) is found in all vertebrates and its type-II molecule is regarded as a fundamental embryonic growth factor during development. We have firstly identified, in this study, a cDNA clone corresponding to IGF-II (flIGF-II) from the adult brain of the teleost, Paralichthys olivaceus. We also examined the tissue expression of flIGF-II in several adult tissues by RT-PCR. The flIGF-II cDNA contained a complete ORF consisting of 215 amino acids and one stop codon. Its molecular characteristics appear to be similar to the previously identified IGF-II molecules, in which a common primary structure exhibiting B, C, A, D, and E domains is evidently observed. This cDNA clone seems to be cleaved at $Ala_{52}$ for the $NH_2$-end signal peptide and appears to produce a 98 amino acid-long E-peptide from the $Arg^{118}$. The functional B-D domain regions, therefore, include 65 amino acids and is able to encode a 7.4-kDa protein. The most prominent structural difference between IGF-I and IGF-II was that the D domain of IGF-II exhibits a two-codon-deleted pattern compared to the 8 amino acid-containing IGF-I. The insulin family signature in the A domain and six cysteins forming three disulfide bridges between the B and A domains were evolutionary-conserved from teleosts to mammalian IGF-II. Interestingly, the E-peptide region appears to provide a distinct hallmark between teleosts in amino acid composition. The flIGF-II shows 85.1% of sequence identity to salmon and trout, 90.6% to tilapia, and 98.4% to perch in amino acid level. In tissue expressions of IGF-II, it is very likely that flIGF-II has a significant expression in the adult brain. However, liver seems to be the main source for IGF-II production, and relatively low signals were observed in the adult muscle and kidney. Taken together, it would be concluded that the functional region for IGF-II mRNA is highly similar in phylogeny and is evolutionary, conserved as a mediator for the growth of vertebrates.

• 대한영양사협회학술지
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• 제7권4호
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• pp.349-360
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• 2001

The purpose of this study was to investigate the nutritional status of school lunch supported students and to provide fundamental data for improving their health. In order to do that, we collected the data for 59 school lunch supported students and 71 not supported students in an elementary school in the Taegu Metropolitan city area. The survey was conducted from February 10 to April 30, 2000. The results are as follows; In relation to their body composition(BMI, Fatmass, Percent Body Fat, Mid-upper Arm Muscle Circumference, Mid-upper Arm Circumference), the school lunch supported students' value was lower than that of the not supported students. In relation to food intake according to the food groups, the school lunch supported students' intake of animal food(p<0.05) and total food(p<0.01)was significantly lower than that of the other students. The average intake of energy for both school lunch supported students and not supported students was below the RDA. Also, it was significantly lower than that of the school lunch supported students in relation to the other students(p<0.05). For protein, calcium, phosphorous, and riboflavin, the school lunch supported students' intake was significantly lower than that of the students who were not supported(p<0.05, p<0.05, p<0.05, p<0.05). There are the classification in relation to the nutrient intake in relation to the RDA standards for that of the school lunch supported students. The criteria used for this study was calorie intake, protein, and niacin. The classifications are for groups below 75%, between 75%～100%, and 100%. The nutrients for which the students were deficient( below 75%) of the RDA were iron, calcium, riboflavin, vitamin C, thiamin, calorie, niacin, vitamin A, protein, and phosphorous in the above order.

• 한국지역사회생활과학회지
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• 제27권3호
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• pp.477-493
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• 2016

This research was conducted to investigate the performance of a weight control program in university students in Daejeon during 3 months from November 2014 to February 2015. This program measured body measurement and composition analysis, nutritional education, and counseling every month. The status of students' weight control was surveyed before and after the program. The participants were 17 males (24.5 years old) and 15 females (20.8 years old). Their weights before the program were 78.2 kg (male) and 57.2 kg (female), whereas after the program, weights were 77.6 kg (male) and 56.2 kg (female). Weight reduction in students was 53.1%, and weight increase was 40.6% by the weight control program. Body mass index (BMI) tended to decrease after the program. Body fat % and muscle masses were not significantly different by program practice. Body image decision of females showed mainly 'normal' status while that of male was 'over fatty constitution', Before and after the program, the most prevalent method of weight control was exercise for all students. The most selected exercise was 'walking and jogging' during the program. For the most effective weight control method, female acknowledged both 'reducing amount of meals' and 'increasing exercise' while males selected 'increasing exercise.' On the side effects of weight control, over 40% of all students chose 'no experience' and 'loss of volition'. The degree of student's goal achievement was largely 20 ~-20% compared with their goal weights. Accordingly, this program didn't show significant effects. For effective weight control, it is recommended to conduct nutritional education. Students can exercise regularly and control diet to sustain a healthy and satisfactory body status.

• 한국축산식품학회지
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• 제34권4호
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• pp.457-463
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• 2014

The effects of feeding of diets based on roughage supplemented with concentrate on the carcass and meat quality attributes of Korean black goats were investigated. The 40 male goats at 5 mon age were divided into four treatment groups; T1 (1.5%), T2 (2.0%), T3 (2.5%) and T4 (ad libitum) feeding of concentrate with ad libitum rice straw. Forty bucks at the end of the feeding were fasted for 12 h before slaughter and were then slaughtered. For carcass measurement, cold buck carcasses were examined after 24 h of chilling at $5^{\circ}C$. The carcasses were chilled in refrigerator at $2{\pm}2^{\circ}C$ for 24 h, then the longissimus dorsi muscle was removed from the carcasses. The samples were separately vacuum packaged, frozen and stored at $-20^{\circ}C$ for up to 1 mon, prior to physicochemical evaluations. Carcass yields were greatly affected by rate of concentrate feeding. T4 showed the heaviest live and carcass weights and the highest dressing and fat percentages (p<0.05). The moisture contents were lower in T4 compared with other treatments, while the crude protein and fat contents were higher (p<0.05). mono-unsaturated fatty acid in T4 was significantly higher than others, which showed the highest percentages of oleic acid (C18:1). For the sensory test, T4 was tenderer and produced better flavor scores than others. Increasing the level of concentrate in the diet resulted in an improvement in growth performance, and carcass and physicochemical characteristics of goat meats.

• 한국축산식품학회지
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• 제35권1호
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• pp.101-107
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• 2015

The aim of this study was to investigate the effects of various marination processes on the quality characteristics of chicken breast prepared with chicken feet gelatin and wheat fiber. The chicken feet gelatin was swollen with hydrochloric solution (0.1 N HCl, pH $1.31{\pm}0.02$) and dehydrated by freeze-drying. The composition (w/w) of the marinade was water (10%), soy sauce (12%), phosphate (0.3%), wheat fiber (1.5%), and chicken feet gelatin (1.5%). Three samples of chicken breast were manufactured with Tumbler (only tumbler), Tenderizer (tenderizer and tumbler), and Injector (injector and tumbler). The water content of the Injector sample was significantly higher than those of the Tumbler and Tenderizer samples (p<0.05). During heating, the lightness of all chicken breasts increased and the redness decreased. The tumbling and cooking yield of the Injector sample were significantly higher than those of the Tumbler and Tenderizer samples (p<0.05). The shear force of the Tenderizer sample was significantly lower than that of the Tumbler and Injector samples (p<0.05). No significant differences, except for color, were observed in the sensory analysis of the samples. Thus, the proper selection of mechanical processing is important to improve the quality characteristics of marinated chicken breast, considering the types of final products.

• 한국보건간호학회지
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• 제18권2호
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• pp.299-311
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• 2004

Purpose: Somatotype drawing developed by Sorensen et al.(1983) has been evaluated as a simple instrument of obesity level without real somatic measuring as height and weight. This study was designed to validate somatotype drawings for obesity assessment by bioelectrical impedance body component analysis. Method: At first questionnaire of somatotype drawing was done. Subjects were measured body component by bioelectrical impedance analysis as weight, BMI(body mass index), WHR(waist-hip ratio), body water, protein mass, mineral mass, body fat mass, skeletal muscle mass, soft lean mass, fat free mass and percent body fat. We evaluated correlations between these data and somatotype drawings and tried to grouping of somatotype drawings with the means of major body component value. Result: The data were collected from 205 college women whose height and weight were $161.2\pm4.8,\;55\pm8.3$. Spearman's correlation coefficients of somatotype drawing were 0.74 with BMI, 0.68 with weight 0.69 with body fat mass, 0.65 with WHR. 0.64 with percent body fat after adiusted age. The grade of somatotype drawings were grouped as 1-2, 3-4, 5-6. 7-9 by BMI, body fat mass, weight, 1, 2-4, 5-6, 7-9 by WHR and 1-2, 3-4, 5-9 by percent body fat(ANOVA and Duncan's method). Conclusion: So quick instrument using somatotype drawings were useful tools for evaluation of obesity level and is applicable to screen degree of body fat in self-administered questionnaire survey.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.1047-1053
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• 2006

The current study was designed to determine the effects of dietary docosahexaenoic acid (DHA) on fatty acid deposition in egg yolk and various tissues of laying Tsaiya ducks, and on the mRNA concentrations of hepatic lipogenesis-related transcription factors. Thirty laying ducks were randomly assigned to three treatments with diets based on corn-soybean meal (ME: 2803 kcal/kg; CP: 17.1%; Ca: 3.4%) supplemented with 0% (control diet), 0.5% or 2% algal DHA oil. The DHA content in egg yolks of the ducks was elevated significantly (p<0.01) with the supplementation of dietary DHA. The DHA percentage of the total fatty acids in the egg yolk of laying ducks was 0.5%, 1.3% and 3.4% for 0%, 0.5% and 2% algal DHA oil treatments, respectively, for the $1^{st}$ week, and 0.5%, 1.5% and 3.3% for the $2^{nd}$ week. Therefore, algal DHA oil can be utilized by laying Tsaiya ducks to enhance the egg-yolk DHA content. The concentrations of triacylglycerol (TG) and cholesterol in plasma of laying Tsaiya ducks were not affected by dietary DHA treatments (p>0.05). The DHA concentration in plasma, liver, and skeletal muscle was increased with the addition of dietary algal DHA oil (p<0.05). The mRNA abundance of sterol regulatory element binding protein 1 (SREBP1) and SREBP2 in the livers of laying Tsaiya ducks was not affected by dietary DHA, suggesting that the expression of these transcription factors is tightly controlled and not sensitive to DHA treatments.